ISAmo1
- Family ISLre2
- Group
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_018024 | ND | Anaerobaculum mobile | Anaerobaculum mobile DSM 13181 |
DNA section
IS Length : 1572 bp
Ends
IR Length : 22/28
IRL : GATAGCGTAAAATATTTGTAGGAGTTTTTTAGGGAGGAAAAATAAAGGGG
IRR : GACCGCGTCAAGTTTTTGTAGGATTTTTAGGAACCACTCCCCGATGTATC
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| GCCAGGGATA | CTTACAGG | TTGGTCCAGG | 8 |
| TTGGCGCGGT | CGATAGATC | AATTTGCCTT | 9 |
| GTGTGTCTAT | CAAATCCTAC | 0 |
DNA sequence
GATAGCGTAAAATATTTGTAGGAGTTTTTTAGGGAGGAAAAATAAAGGGGCCGCTCCTTTTGGTAGAATCTTTGTAGCACACAAAAATTCATAAAGGAGG
GTCCCAACTTGAACAAGGTTATTTTACTACAAATTGTCTCAAACTTCATCTCCGAGATATTGAAGTTTTTTTGCAGTTCCCATGTCAGGACGCTGGCTGA
AATAGAAGATGAGCTTTTTCGCATGACGAAGGCCTTCATCCGGGAGATCGTAAAGGCTTACCTTGAGCTTGCAGACGAAGCCATATTAAAGGACAAAACG
TCTCGAAAGCAAAGAGGATTGGTCGTAGAAAGGCGTGATGACAAAAGAAGCGTTTATACCATATTTGGAGATATATCCTTTGACAGGACCTACTACTTTG
ATAAAAGTCATGATAAATACGTATACCCCTTAGACGAAGCCCTGGGGTTGGATAAATACGAAAGGATAAGCAAAACAGTGACGGTAAAGCTGGTAGAGAC
GGCAGGCCAGGTATCTTACGCTAAAAGTTCTTCAAACGTGACTAGTGGTGAGTTATCCAAACAAACCGTCAAAAACAAGATACACAGCCTAAACCTGGAG
GCCTTAAAGACAAAGATCCCCGAGAAAAGAAGCGCGCATGTATTGCACATAGATGCAGATGAAGACCACGTATCCTTACAGGAGGGCAGAAATGTGAATC
TTCCTTTGATTTGCATATACGAGGGCACCTTTAAAGACGGATCTAAAAATAGATGTATAAACCCCATATACATGAGCGGTTACGGAAAAGACGCAGACGA
GTTTTGGCTTGAGGTAACAGATAGGATATACGATCTTTACGACCCGGAGGACATAAAGGACATATACATCCATGGAGATGGTGCAAATTGGATAAGACAG
GGGATAAACTGGCTGCCCGAGTCAAAGTTGGTGCTCGATAAATTCCACCTAAATAAAGCGATCCTTGAATCCACGGCAAGGCAGCCTGAAAAAAGACGTT
ATATATACAGGGCCATAAATACAAATGACCTTAACTCCTTTAAAAAGATTAGCTTTGAGATGTTAAACGATGCCCTGGATGAAAAGGAAAGAAGGAGGAT
AAAAGATTTCAGGAGGTATATAACCAACAACTGGCAGTCTATAACGATTCGAAACGAAGAGGACTGTGGCAGCTCAAGCCCTGAAGGTCACGTAAGCCAC
GTACTTTCTTCTAGGTTAAGTTCCAGGCCTATGGCCTGGAGCAGGAAGGGCTTAAAGGCCATGTCTGCTTTAAGGGCTTACATCTGTAGCGGTGGCATGG
TGACGTCAGAACAGGTAAAGAAAAAAGACCAAGAAGGTGAGAATGCGGACAAAAGACACAAATTCACCTTGAACCTGGGGGATATCTTCGGTTCCGTTGC
AAGTGAGCTTGGATGCATAACGGTACTTAAAACGGGAAAAGTAACGCCTTTATACACTTCTTTAAAGGGCATATGTCATAGCGGGTTTGATTTTTAGCTG
ACTCGCAGACGTTACCCTTTAAGATACATCGGGGAGTGGTTCCTAAAAATCCTACAAAAACTTGACGCGGTC
GTCCCAACTTGAACAAGGTTATTTTACTACAAATTGTCTCAAACTTCATCTCCGAGATATTGAAGTTTTTTTGCAGTTCCCATGTCAGGACGCTGGCTGA
AATAGAAGATGAGCTTTTTCGCATGACGAAGGCCTTCATCCGGGAGATCGTAAAGGCTTACCTTGAGCTTGCAGACGAAGCCATATTAAAGGACAAAACG
TCTCGAAAGCAAAGAGGATTGGTCGTAGAAAGGCGTGATGACAAAAGAAGCGTTTATACCATATTTGGAGATATATCCTTTGACAGGACCTACTACTTTG
ATAAAAGTCATGATAAATACGTATACCCCTTAGACGAAGCCCTGGGGTTGGATAAATACGAAAGGATAAGCAAAACAGTGACGGTAAAGCTGGTAGAGAC
GGCAGGCCAGGTATCTTACGCTAAAAGTTCTTCAAACGTGACTAGTGGTGAGTTATCCAAACAAACCGTCAAAAACAAGATACACAGCCTAAACCTGGAG
GCCTTAAAGACAAAGATCCCCGAGAAAAGAAGCGCGCATGTATTGCACATAGATGCAGATGAAGACCACGTATCCTTACAGGAGGGCAGAAATGTGAATC
TTCCTTTGATTTGCATATACGAGGGCACCTTTAAAGACGGATCTAAAAATAGATGTATAAACCCCATATACATGAGCGGTTACGGAAAAGACGCAGACGA
GTTTTGGCTTGAGGTAACAGATAGGATATACGATCTTTACGACCCGGAGGACATAAAGGACATATACATCCATGGAGATGGTGCAAATTGGATAAGACAG
GGGATAAACTGGCTGCCCGAGTCAAAGTTGGTGCTCGATAAATTCCACCTAAATAAAGCGATCCTTGAATCCACGGCAAGGCAGCCTGAAAAAAGACGTT
ATATATACAGGGCCATAAATACAAATGACCTTAACTCCTTTAAAAAGATTAGCTTTGAGATGTTAAACGATGCCCTGGATGAAAAGGAAAGAAGGAGGAT
AAAAGATTTCAGGAGGTATATAACCAACAACTGGCAGTCTATAACGATTCGAAACGAAGAGGACTGTGGCAGCTCAAGCCCTGAAGGTCACGTAAGCCAC
GTACTTTCTTCTAGGTTAAGTTCCAGGCCTATGGCCTGGAGCAGGAAGGGCTTAAAGGCCATGTCTGCTTTAAGGGCTTACATCTGTAGCGGTGGCATGG
TGACGTCAGAACAGGTAAAGAAAAAAGACCAAGAAGGTGAGAATGCGGACAAAAGACACAAATTCACCTTGAACCTGGGGGATATCTTCGGTTCCGTTGC
AAGTGAGCTTGGATGCATAACGGTACTTAAAACGGGAAAAGTAACGCCTTTATACACTTCTTTAAAGGGCATATGTCATAGCGGGTTTGATTTTTAGCTG
ACTCGCAGACGTTACCCTTTAAGATACATCGGGGAGTGGTTCCTAAAAATCCTACAAAAACTTGACGCGGTC
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1389 bp | 462 aa | 109 | 1497 | + | No |
Chemistry : DDE
ORF sequence :
MNKVILLQIVSNFISEILKFFCSSHVRTLAEIEDELFRMTKAFIREIVKAYLELADEAILKDKTSRKQRGLVVERRDDKRSVYTIFGDISFDRTYYFDKS
HDKYVYPLDEALGLDKYERISKTVTVKLVETAGQVSYAKSSSNVTSGELSKQTVKNKIHSLNLEALKTKIPEKRSAHVLHIDADEDHVSLQEGRNVNLPL
ICIYEGTFKDGSKNRCINPIYMSGYGKDADEFWLEVTDRIYDLYDPEDIKDIYIHGDGANWIRQGINWLPESKLVLDKFHLNKAILESTARQPEKRRYIY
RAINTNDLNSFKKISFEMLNDALDEKERRRIKDFRRYITNNWQSITIRNEEDCGSSSPEGHVSHVLSSRLSSRPMAWSRKGLKAMSALRAYICSGGMVTS
EQVKKKDQEGENADKRHKFTLNLGDIFGSVASELGCITVLKTGKVTPLYTSLKGICHSGFDF
HDKYVYPLDEALGLDKYERISKTVTVKLVETAGQVSYAKSSSNVTSGELSKQTVKNKIHSLNLEALKTKIPEKRSAHVLHIDADEDHVSLQEGRNVNLPL
ICIYEGTFKDGSKNRCINPIYMSGYGKDADEFWLEVTDRIYDLYDPEDIKDIYIHGDGANWIRQGINWLPESKLVLDKFHLNKAILESTARQPEKRRYIY
RAINTNDLNSFKKISFEMLNDALDEKERRRIKDFRRYITNNWQSITIRNEEDCGSSSPEGHVSHVLSSRLSSRPMAWSRKGLKAMSALRAYICSGGMVTS
EQVKKKDQEGENADKRHKFTLNLGDIFGSVASELGCITVLKTGKVTPLYTSLKGICHSGFDF
Blast result :
Comments
ISAmo1 is 91% aa similar to ISAnhy1.
IS of ISLre2 family preferentially insert in intergenic region, 15 to 17 bp upstream the -35 box of sigmaA promoters. [2][3][4]
IS of ISLre2 family preferentially insert in intergenic region, 15 to 17 bp upstream the -35 box of sigmaA promoters. [2][3][4]
References
1] Romain Guérillot and Philippe Glaser (2013) Direct submission.
2] Brochet M, Da Cunha V, Couvé E, Rusniok C, Trieu-Cuot P, and Glaser P, P.(2009) Mol Microbiol. 71(4):948-59;
3] Guérillot R, Da Cunha V, Sauvage E, Bouchier C, Glaser P, (2013) Modular evolution of TnGBSs, a new family of ICEs associating IS transposition, plasmid replication and conjugation for their spreading (submitted)
4] Guérillot R, Glaser P, (2013) Diversity of bacterial prokaryotic mutator like transposase, association with conjugation conjugative elements and insertion specificity (on preparation).
2] Brochet M, Da Cunha V, Couvé E, Rusniok C, Trieu-Cuot P, and Glaser P, P.(2009) Mol Microbiol. 71(4):948-59;
3] Guérillot R, Da Cunha V, Sauvage E, Bouchier C, Glaser P, (2013) Modular evolution of TnGBSs, a new family of ICEs associating IS transposition, plasmid replication and conjugation for their spreading (submitted)
4] Guérillot R, Glaser P, (2013) Diversity of bacterial prokaryotic mutator like transposase, association with conjugation conjugative elements and insertion specificity (on preparation).