ISAmde3
- Family ISLre2
- Group
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_013385 | ND | Ammonifex degensii | Ammonifex degensii KC4 |
DNA section
IS Length : 1586 bp
Ends
IR Length : 18/23
IRL : GTTAGTGTAAAGTTACTGTAGGAATTTTGCAGGAGCTAAGGAAGGGGAGA
IRR : GATCGTGTCAACCTACTGTAGGAGTCTCGAGCCCCACCGTCCCCGGGCCA
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| CTTCTTGACC | TAGAGCCTC | CGCTTGCTTG | 9 |
| ACCTTTTTCC | CACAGGCGGC | 0 |
DNA sequence
GTTAGTGTAAAGTTACTGTAGGAATTTTGCAGGAGCTAAGGAAGGGGAGAAAGAAGAGAGACCTCACCGTCCTTGCAACGGACGAGTAGCTGCTCAAACA
ACTACTCAAGAGAGGTGAGGTCTCTATGCAGATTGTAAAACAGATTTGGGAAAAGTTCCAGAGGGTAGCGGAGTTAACGTTTGAGGTATTAGAGAACGGG
ATTGATTTTATAAGCTTTCAGGAGAAGCTCCGGCGGGAACTCGACAGCTTAGGGCAAGAGATCCTGAGGGAAGTTTTAGAGGCGAAAGACGCTTATTTAC
GGGAACATCGGGAAGAGCGGCCTGGCTGGCGAGTAGAGCGGCGAAACGACACTAAAGGGGTGCTCACCCTTTTCGGTCTGGTCAGCTACAAGCGGACCTA
TTACCGGCATAAAGAGACTGGGGAGCGGGCGTACTTAATAGACCGGTTGGTAGGTTACGGGCCGCACGCGCGGGTTGATCCTCTGGTTAAAGCACAAGCC
TTAGAAGAAGCGGTAGAGCTTTCCTATCGGAAGAGTGGCGAGAGGGTAGGGAGAGGCAATCGGGAGGTTGTGCTAAGTGGCGAAGTGGTGAAGAGGGTGA
TTCACAACTTTACACCGGAAGAACTGCCCGAACCACCCAAAGAGAAGCGCCGGGTAAAGGTTCTTTATGTAGAGGCGGATGAAGACCACGTAGCAGGTCA
GGACAGAAAGAAGTACTTACCCCGGCTGGTATACATCCACGAGGGGAAAGAGAAGGCGAGGAAAGACAGATACAGACTCAAGCGGCCTTATTACCTGGGA
GGGCTTTACTCAGATACGGATGAGCTGTGGTTCGAGGTGTTGAGCTACATCGAGGAGCAGTACGAATTATCTTCTGTGGAGCAGATTTATCTCTGCGGTG
ACGGAGACAGGTGGATAAAGAAAGGGCTGGAGTTTCTACCGAGGAGCGTTTTTGTCTTAGATCTTTTTCACCTGGACAAGTACCTGGTGGCTGCTTTGGG
GCGTGATAGTGATGGTTATAGAGAGATTTGGGCGGCCTTATGGGAGGGTGATGAGGTAAAGGTACAGGCGATTTTGCGGCGGGCAGGCAAAGCTGCGGCG
AGTCCTGCACGGAAGGAAGCGGTACGGGATTGCCGACGTTACATCCGGCAGAACTGGGAGGGGATAATGGCCTACCAGCTTTATCCCGGGGCGGAGTTGG
GGGTAAGTGCGGAGGCGCACGTAAGTCATCTCTTCTCGGCACGGTTATCATCCCGACCGATGGCTTGGAGTGCTTGTGGGGTAGACCGGATGGCCCGGTT
GCGGGTGGCGAAGGCGAATGGTGTTTCCTTGCGGGAGCAATATGTAGCCCGATGGAGGGAGGGCTTAAAGGCTTTAGAGATCGATAAGGCAGCCATTGAG
GAAGAGAGGCAGAGGCTAAGGAAAGTATCGGGTGAGGTGTTCGATAATCTTCCCGCTCTACGGGGTCCGGTAACATCATTAACCAGAGCCCTCAAAGCTT
TGAGCCGGAACATCGATCTTCTTTGGTAGTTCGTCCTGGCCCGGGGACGGTGGGGCTCGAGACTCCTACAGTAGGTTGACACGATC
ACTACTCAAGAGAGGTGAGGTCTCTATGCAGATTGTAAAACAGATTTGGGAAAAGTTCCAGAGGGTAGCGGAGTTAACGTTTGAGGTATTAGAGAACGGG
ATTGATTTTATAAGCTTTCAGGAGAAGCTCCGGCGGGAACTCGACAGCTTAGGGCAAGAGATCCTGAGGGAAGTTTTAGAGGCGAAAGACGCTTATTTAC
GGGAACATCGGGAAGAGCGGCCTGGCTGGCGAGTAGAGCGGCGAAACGACACTAAAGGGGTGCTCACCCTTTTCGGTCTGGTCAGCTACAAGCGGACCTA
TTACCGGCATAAAGAGACTGGGGAGCGGGCGTACTTAATAGACCGGTTGGTAGGTTACGGGCCGCACGCGCGGGTTGATCCTCTGGTTAAAGCACAAGCC
TTAGAAGAAGCGGTAGAGCTTTCCTATCGGAAGAGTGGCGAGAGGGTAGGGAGAGGCAATCGGGAGGTTGTGCTAAGTGGCGAAGTGGTGAAGAGGGTGA
TTCACAACTTTACACCGGAAGAACTGCCCGAACCACCCAAAGAGAAGCGCCGGGTAAAGGTTCTTTATGTAGAGGCGGATGAAGACCACGTAGCAGGTCA
GGACAGAAAGAAGTACTTACCCCGGCTGGTATACATCCACGAGGGGAAAGAGAAGGCGAGGAAAGACAGATACAGACTCAAGCGGCCTTATTACCTGGGA
GGGCTTTACTCAGATACGGATGAGCTGTGGTTCGAGGTGTTGAGCTACATCGAGGAGCAGTACGAATTATCTTCTGTGGAGCAGATTTATCTCTGCGGTG
ACGGAGACAGGTGGATAAAGAAAGGGCTGGAGTTTCTACCGAGGAGCGTTTTTGTCTTAGATCTTTTTCACCTGGACAAGTACCTGGTGGCTGCTTTGGG
GCGTGATAGTGATGGTTATAGAGAGATTTGGGCGGCCTTATGGGAGGGTGATGAGGTAAAGGTACAGGCGATTTTGCGGCGGGCAGGCAAAGCTGCGGCG
AGTCCTGCACGGAAGGAAGCGGTACGGGATTGCCGACGTTACATCCGGCAGAACTGGGAGGGGATAATGGCCTACCAGCTTTATCCCGGGGCGGAGTTGG
GGGTAAGTGCGGAGGCGCACGTAAGTCATCTCTTCTCGGCACGGTTATCATCCCGACCGATGGCTTGGAGTGCTTGTGGGGTAGACCGGATGGCCCGGTT
GCGGGTGGCGAAGGCGAATGGTGTTTCCTTGCGGGAGCAATATGTAGCCCGATGGAGGGAGGGCTTAAAGGCTTTAGAGATCGATAAGGCAGCCATTGAG
GAAGAGAGGCAGAGGCTAAGGAAAGTATCGGGTGAGGTGTTCGATAATCTTCCCGCTCTACGGGGTCCGGTAACATCATTAACCAGAGCCCTCAAAGCTT
TGAGCCGGAACATCGATCTTCTTTGGTAGTTCGTCCTGGCCCGGGGACGGTGGGGCTCGAGACTCCTACAGTAGGTTGACACGATC
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1404 bp | 467 aa | 126 | 1529 | + | No |
Chemistry : DDE
ORF sequence :
MQIVKQIWEKFQRVAELTFEVLENGIDFISFQEKLRRELDSLGQEILREVLEAKDAYLREHREERPGWRVERRNDTKGVLTLFGLVSYKRTYYRHKETGE
RAYLIDRLVGYGPHARVDPLVKAQALEEAVELSYRKSGERVGRGNREVVLSGEVVKRVIHNFTPEELPEPPKEKRRVKVLYVEADEDHVAGQDRKKYLPR
LVYIHEGKEKARKDRYRLKRPYYLGGLYSDTDELWFEVLSYIEEQYELSSVEQIYLCGDGDRWIKKGLEFLPRSVFVLDLFHLDKYLVAALGRDSDGYRE
IWAALWEGDEVKVQAILRRAGKAAASPARKEAVRDCRRYIRQNWEGIMAYQLYPGAELGVSAEAHVSHLFSARLSSRPMAWSACGVDRMARLRVAKANGV
SLREQYVARWREGLKALEIDKAAIEEERQRLRKVSGEVFDNLPALRGPVTSLTRALKALSRNIDLLW
RAYLIDRLVGYGPHARVDPLVKAQALEEAVELSYRKSGERVGRGNREVVLSGEVVKRVIHNFTPEELPEPPKEKRRVKVLYVEADEDHVAGQDRKKYLPR
LVYIHEGKEKARKDRYRLKRPYYLGGLYSDTDELWFEVLSYIEEQYELSSVEQIYLCGDGDRWIKKGLEFLPRSVFVLDLFHLDKYLVAALGRDSDGYRE
IWAALWEGDEVKVQAILRRAGKAAASPARKEAVRDCRRYIRQNWEGIMAYQLYPGAELGVSAEAHVSHLFSARLSSRPMAWSACGVDRMARLRVAKANGV
SLREQYVARWREGLKALEIDKAAIEEERQRLRKVSGEVFDNLPALRGPVTSLTRALKALSRNIDLLW
Blast result :
Comments
ISAmde3 is 91% aa similar to ISAmde2.
IS of ISLre2 family preferentially insert in intergenic region, 15 to 17 bp upstream the -35 box of sigmaA promoters. [2][3][4]
IS of ISLre2 family preferentially insert in intergenic region, 15 to 17 bp upstream the -35 box of sigmaA promoters. [2][3][4]
References
1] Romain Guérillot and Philippe Glaser (2013) Direct submission.
2] Brochet M, Da Cunha V, Couvé E, Rusniok C, Trieu-Cuot P, and Glaser P, P.(2009) Mol Microbiol. 71(4):948-59;
3] Guérillot R, Da Cunha V, Sauvage E, Bouchier C, Glaser P, (2013) Modular evolution of TnGBSs, a new family of ICEs associating IS transposition, plasmid replication and conjugation for their spreading (submitted)
4] Guérillot R, Glaser P, (2013) Diversity of bacterial prokaryotic mutator like transposase, association with conjugation conjugative elements and insertion specificity (on preparation).
2] Brochet M, Da Cunha V, Couvé E, Rusniok C, Trieu-Cuot P, and Glaser P, P.(2009) Mol Microbiol. 71(4):948-59;
3] Guérillot R, Da Cunha V, Sauvage E, Bouchier C, Glaser P, (2013) Modular evolution of TnGBSs, a new family of ICEs associating IS transposition, plasmid replication and conjugation for their spreading (submitted)
4] Guérillot R, Glaser P, (2013) Diversity of bacterial prokaryotic mutator like transposase, association with conjugation conjugative elements and insertion specificity (on preparation).