ISAmde4
- Family ISLre2
- Group
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_013385 | ND | Ammonifex degensii | Ammonifex degensii KC4 |
DNA section
IS Length : 1584 bp
Ends
IR Length : 21/25
IRL : GTTAGTGTAAAGTTACTGTAGGAGTTTTGCAGGAGCTGAGGAGGAAAAAA
IRR : GATCGTGTCAACCTACTGTAGGAGTCTCGATCCCCACCGTCCCTGAGCCA
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| GCAAGCGAAG | CGCGGGAAAA | 0 |
DNA sequence
GTTAGTGTAAAGTTACTGTAGGAGTTTTGCAGGAGCTGAGGAGGAAAAAACGAAGAGAGACCTCACCGTCCTTCGAAGGGACGAGCAGTTGACAATCCAA
CCACTCAGGAGGGTGAGGTCTCTATGCAGATTATAAAGCAGATTTGGGAGAAGTTCCAGAGGGTAGCGGAGCTAACGTTAAAGATTCTGGAGGAAGGGAT
CGACCTTTTAAGCTTCGAGGAGAAGCTCTGGCAGGAGCTTAAAAGCTTAGGAAAAGAGATCCTGAGGGAAGTTTTGGAGGCAAAAGACGCTTATTTACGG
GAGCACCGGGAAAAGCGGCCTGGCTGGCAAGTAGAGCGGAGGAATGATCCCAAGGAAGTGCTCACCCTTTTCGGTCTGGTCAGCTACAAGCGGACCTATT
ACCGGCACAAAGAGACTGGGGAGCGGGCATACTTAATAGACCGTTTGGTGGGTTACGGGCCGCATACGCGGGTTGACCCTCTGGTCAAGGCACAAGTTTT
AGAAGAAGCAGTAGAGCTTTCTTACCGGAAAAGTGGGGAAAGAGTAGGGAAAGGTAGCCAGGAAGTTGTACTGAGTGGCGAGGCTGTGGAGAAGGTAGTG
CACGACTTTATTCCTGAAGAGCTGCCCGAACCACCCAACGATAAGCGCCGGGTAAAGGTTCTTTATGTGGAGGCGGATGAAGACCACGTAGCAGGTCAGG
ACGGGAAGAAGTACTTACCCTGGCTGGTTTACATCCACGAGGGGAAAGAGAAGGTGGGGAAAGACAGATACAGACTCAAGCGGCCTTATTACTTGGGAGG
GCTTTACTCAGATACGGATGAGCTGTGGTTCGAGGTGTTGAGCTACATCGAGGGGCAGTACGAATTATCTCCTGTGGAGCGGACTTATCTCTGCGGTGAC
GGAGACAGGTGGATAAAGAAAGGGCTGGAGTTTCTACCGAGGAGCGTTTTTGTCTTAGATCTTTTTCACCTGGACAAGTACCTGGTGGCTGCTTTGGGGC
GTGATAGTGATGGTTATAGAGAGATTTGGGCGGCCTTATGGGATGGTGATGAGGTAAAGGTACAGGCGATTTTGCGGCGGGCAGGCAAAGCTGCGGCGAG
TCCTGCACGGAAGGGAGCAGTACGGGATTGCCGATGTTACATCCGGCAGAACTGGGAGGGGATAACGGCATACCGGCTTTACCCGGAGGCGCAGTTAGGG
GTGAGCGCGGAGGCGCACGTAAGCCACCTGTACTCGGCGCGGTTGTCGTCGCGGCCGATGGCCTGGAGTGCTTGTGGGGTAGACCGGATGACCCGGTTGC
GGGTGGCGAAGGCGAATGGTGTTTCCTTGCGGGAGCAATATGTAGCCCGATGGAGGGAGGGCTTAAAGGCTTTAGAGATCGATAAGGCAGCCATTGAGGA
AGAGAGGCAGAGGCTAAGGAAAGTATCGGGTGAGGTGTTCGATAATCTTCCCGCATTACGGGGTCCGGTAACATCATTAACCAGAGCCCTCAAAGCTTTG
AGCCGGAACATCGATCTTCTCTGGTAGTTCGTCCTGGCTCAGGGACGGTGGGGATCGAGACTCCTACAGTAGGTTGACACGATC
CCACTCAGGAGGGTGAGGTCTCTATGCAGATTATAAAGCAGATTTGGGAGAAGTTCCAGAGGGTAGCGGAGCTAACGTTAAAGATTCTGGAGGAAGGGAT
CGACCTTTTAAGCTTCGAGGAGAAGCTCTGGCAGGAGCTTAAAAGCTTAGGAAAAGAGATCCTGAGGGAAGTTTTGGAGGCAAAAGACGCTTATTTACGG
GAGCACCGGGAAAAGCGGCCTGGCTGGCAAGTAGAGCGGAGGAATGATCCCAAGGAAGTGCTCACCCTTTTCGGTCTGGTCAGCTACAAGCGGACCTATT
ACCGGCACAAAGAGACTGGGGAGCGGGCATACTTAATAGACCGTTTGGTGGGTTACGGGCCGCATACGCGGGTTGACCCTCTGGTCAAGGCACAAGTTTT
AGAAGAAGCAGTAGAGCTTTCTTACCGGAAAAGTGGGGAAAGAGTAGGGAAAGGTAGCCAGGAAGTTGTACTGAGTGGCGAGGCTGTGGAGAAGGTAGTG
CACGACTTTATTCCTGAAGAGCTGCCCGAACCACCCAACGATAAGCGCCGGGTAAAGGTTCTTTATGTGGAGGCGGATGAAGACCACGTAGCAGGTCAGG
ACGGGAAGAAGTACTTACCCTGGCTGGTTTACATCCACGAGGGGAAAGAGAAGGTGGGGAAAGACAGATACAGACTCAAGCGGCCTTATTACTTGGGAGG
GCTTTACTCAGATACGGATGAGCTGTGGTTCGAGGTGTTGAGCTACATCGAGGGGCAGTACGAATTATCTCCTGTGGAGCGGACTTATCTCTGCGGTGAC
GGAGACAGGTGGATAAAGAAAGGGCTGGAGTTTCTACCGAGGAGCGTTTTTGTCTTAGATCTTTTTCACCTGGACAAGTACCTGGTGGCTGCTTTGGGGC
GTGATAGTGATGGTTATAGAGAGATTTGGGCGGCCTTATGGGATGGTGATGAGGTAAAGGTACAGGCGATTTTGCGGCGGGCAGGCAAAGCTGCGGCGAG
TCCTGCACGGAAGGGAGCAGTACGGGATTGCCGATGTTACATCCGGCAGAACTGGGAGGGGATAACGGCATACCGGCTTTACCCGGAGGCGCAGTTAGGG
GTGAGCGCGGAGGCGCACGTAAGCCACCTGTACTCGGCGCGGTTGTCGTCGCGGCCGATGGCCTGGAGTGCTTGTGGGGTAGACCGGATGACCCGGTTGC
GGGTGGCGAAGGCGAATGGTGTTTCCTTGCGGGAGCAATATGTAGCCCGATGGAGGGAGGGCTTAAAGGCTTTAGAGATCGATAAGGCAGCCATTGAGGA
AGAGAGGCAGAGGCTAAGGAAAGTATCGGGTGAGGTGTTCGATAATCTTCCCGCATTACGGGGTCCGGTAACATCATTAACCAGAGCCCTCAAAGCTTTG
AGCCGGAACATCGATCTTCTCTGGTAGTTCGTCCTGGCTCAGGGACGGTGGGGATCGAGACTCCTACAGTAGGTTGACACGATC
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1404 bp | 467 aa | 124 | 1527 | + | No |
Chemistry : DDE
ORF sequence :
MQIIKQIWEKFQRVAELTLKILEEGIDLLSFEEKLWQELKSLGKEILREVLEAKDAYLREHREKRPGWQVERRNDPKEVLTLFGLVSYKRTYYRHKETGE
RAYLIDRLVGYGPHTRVDPLVKAQVLEEAVELSYRKSGERVGKGSQEVVLSGEAVEKVVHDFIPEELPEPPNDKRRVKVLYVEADEDHVAGQDGKKYLPW
LVYIHEGKEKVGKDRYRLKRPYYLGGLYSDTDELWFEVLSYIEGQYELSPVERTYLCGDGDRWIKKGLEFLPRSVFVLDLFHLDKYLVAALGRDSDGYRE
IWAALWDGDEVKVQAILRRAGKAAASPARKGAVRDCRCYIRQNWEGITAYRLYPEAQLGVSAEAHVSHLYSARLSSRPMAWSACGVDRMTRLRVAKANGV
SLREQYVARWREGLKALEIDKAAIEEERQRLRKVSGEVFDNLPALRGPVTSLTRALKALSRNIDLLW
RAYLIDRLVGYGPHTRVDPLVKAQVLEEAVELSYRKSGERVGKGSQEVVLSGEAVEKVVHDFIPEELPEPPNDKRRVKVLYVEADEDHVAGQDGKKYLPW
LVYIHEGKEKVGKDRYRLKRPYYLGGLYSDTDELWFEVLSYIEGQYELSPVERTYLCGDGDRWIKKGLEFLPRSVFVLDLFHLDKYLVAALGRDSDGYRE
IWAALWDGDEVKVQAILRRAGKAAASPARKGAVRDCRCYIRQNWEGITAYRLYPEAQLGVSAEAHVSHLYSARLSSRPMAWSACGVDRMTRLRVAKANGV
SLREQYVARWREGLKALEIDKAAIEEERQRLRKVSGEVFDNLPALRGPVTSLTRALKALSRNIDLLW
Blast result :
Comments
ISAmde4 is 94% aa similar to ISAmde3.
IS of ISLre2 family preferentially insert in intergenic region, 15 to 17 bp upstream the -35 box of sigmaA promoters. [2][3][4]
IS of ISLre2 family preferentially insert in intergenic region, 15 to 17 bp upstream the -35 box of sigmaA promoters. [2][3][4]
References
1] Romain Guérillot and Philippe Glaser (2013) Direct submission.
2] Brochet M, Da Cunha V, Couvé E, Rusniok C, Trieu-Cuot P, and Glaser P, P.(2009) Mol Microbiol. 71(4):948-59;
3] Guérillot R, Da Cunha V, Sauvage E, Bouchier C, Glaser P, (2013) Modular evolution of TnGBSs, a new family of ICEs associating IS transposition, plasmid replication and conjugation for their spreading (submitted)
4] Guérillot R, Glaser P, (2013) Diversity of bacterial prokaryotic mutator like transposase, association with conjugation conjugative elements and insertion specificity (on preparation).
2] Brochet M, Da Cunha V, Couvé E, Rusniok C, Trieu-Cuot P, and Glaser P, P.(2009) Mol Microbiol. 71(4):948-59;
3] Guérillot R, Da Cunha V, Sauvage E, Bouchier C, Glaser P, (2013) Modular evolution of TnGBSs, a new family of ICEs associating IS transposition, plasmid replication and conjugation for their spreading (submitted)
4] Guérillot R, Glaser P, (2013) Diversity of bacterial prokaryotic mutator like transposase, association with conjugation conjugative elements and insertion specificity (on preparation).