ISLke1

  • Family ISLre2
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
NC_015602 ND Lactobacillus kefiranofaciens
Lactobacillus kefiranofaciens ZW3
DNA section
IS Length : 1599 bp

Ends


IR Length : 19/24

IRL : CTAAGTGTATAATTTTTTGCGGTAAGTTGAATAATTAATTAAAAAAGCTC
IRR : GTAAGTGTCAATATTTTTGCGGTAGGCTATTAACCATTAGTCTGACCTTA

Insertion site


Left flankDirect repeatRight flankDR Length
AAAACAAGAACTGAAGGCCTCAGCTCTTG9
TTTTTGTTTGCTTTTAGGAATATAAAATG9

DNA sequence

CTAAGTGTATAATTTTTTGCGGTAAGTTGAATAATTAATTAAAAAAGCTCAAGTCCTTTGAATTTAGACTTGAGCTCAAACAAAAAGACCAGTAGTCTGA
TGTTAAGAACAAAAAACACATTAGAAGCTGGTCTTTTATGGAATCTATTATAACTGATATCGTGAAAATTATTAAGTCTGAAAATAATGTTATTGCTCGT
GAAAAGGCATTAATGTGCTACTTCTTTGGTTTGATTAGAGAATTAATAAAATTAGCACTAGAAGAAGTTGATGCCGGCTTAGTTGAAGAAACTAAAAAGC
AAGGCTATCAGATTGAAAAGAAAAATAAGCGCTCAGTTGTGACTGCCTTTGGTGAAATCAGCTATTGGCGCAGAAGATATGCTTGTCCTGGCAAAAAGGC
TAAATATCCACTGGATAAGCTGATGGGCTATGACAAATATAAGCGTTACAGTGTTTTGGCAGTAAAGGATATTTTGCAGGTTAGCGCCGTGGCAACTTAC
CGCAATACTGCTTTAGCTGTTAATACTCTCTCCTGTTTCAAGATTAGTCACAGCCAAGTTGGCAAGCTGATTGTGCAAGCAGGCAAACAGATTAAAGTAC
AGCAGCAGTCTGAGGAAAGATACGATGGTATTACTCAAAAGAAAAAGGTGCCAGTGCTTTATCTTGAAGGCGATGGCGTTGTGATCAAGGGCACCAAGAA
GCGGCTAGAGTTTCATCGCTATCAGGTCTGCGAGGATATTATTAATTTAAGCAAAACGCGCAGGAAAAGAGTACAGGCCAAAGAGTTTGTTTCTTTAAGC
CGCTTGGATGCTTTACAAGAAATTAAAGCTTATTTAGCCAATCCCTATGACTTAAGCAATACTTTAATCATCAGTAATGCCGATGGCGGTGCTGGTTATG
CCAAGAAAGACTTTGATGAAATAGTTGGCTATTGCCGGCAGCACGAACCCTTCTTGGATGTCTTTCACTTGAATAAAAAGATCAAAGATCGTCTAAGCTT
CATGCCGGCCATGCAAGGCAAATTGATCAGTGCAGTTGAGTTCAAGTATGATCGCCACTTGACTGACGTGATTTTAGACACGATTGAAAGCAATCTGGTT
GATGAGCTGAATACGCCAGAAAATCATGAAAACCTAAAGCGGCTGCGCAGCTATCTTCATCGTTGCTGGGTGGATATTAAGCCGTTTAAGATGCGTCATT
TGTCCGTAATTAAGGCAATTGGCTGCTGTGAGAGCAATCACCGTAAATATACTTATCGCGTCAAAGGGCAAGGAAAATACTGGTCAGAAGATGGTGCCGA
AGGAATTCTGCGTGTGCTGACTTGCATTAAAAACAAAGAGCTGGAATACTGGCTTAGCAGTGAATTTGCAGGTGGGCAGCTGAACATTGATGATCAAAAA
GACCTGAAAGGAGTGGTTCGTGCCAGCCTAAGAAAGACGCATGAAGCTCATCTGGGCATTCATCACGGCGCCATTGAAAGCTTAACAGCAGGTCATAGCT
ATTTGGATGATTTCGGCAGAAAATTAAGTCAAATAAATATTTAAGAAATTAAGGTCAGACTAATGGTTAATAGCCTACCGCAAAAATATTGACACTTAC
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
1407 bp468 aa1381544+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MESIITDIVKIIKSENNVIAREKALMCYFFGLIRELIKLALEEVDAGLVEETKKQGYQIEKKNKRSVVTAFGEISYWRRRYACPGKKAKYPLDKLMGYDK
YKRYSVLAVKDILQVSAVATYRNTALAVNTLSCFKISHSQVGKLIVQAGKQIKVQQQSEERYDGITQKKKVPVLYLEGDGVVIKGTKKRLEFHRYQVCED
IINLSKTRRKRVQAKEFVSLSRLDALQEIKAYLANPYDLSNTLIISNADGGAGYAKKDFDEIVGYCRQHEPFLDVFHLNKKIKDRLSFMPAMQGKLISAV
EFKYDRHLTDVILDTIESNLVDELNTPENHENLKRLRSYLHRCWVDIKPFKMRHLSVIKAIGCCESNHRKYTYRVKGQGKYWSEDGAEGILRVLTCIKNK
ELEYWLSSEFAGGQLNIDDQKDLKGVVRASLRKTHEAHLGIHHGAIESLTAGHSYLDDFGRKLSQINI

 

Blast result :
Comments
ISLke1 is 96% aa similar to ISLhe10.
ISs of ISLre2 family preferentially insert in intergenic region, 15 to 17 bp upstream the -35 box of sigmaA promoters. [2][3][4]
References
1] Romain Guérillot and Philippe Glaser (2013) Direct submission.
2] Brochet M, Da Cunha V, Couvé E, Rusniok C, Trieu-Cuot P, and Glaser P, P.(2009) Mol Microbiol. 71(4):948-59;
3] Guérillot R, Da Cunha V, Sauvage E, Bouchier C, Glaser P, (2013) Modular evolution of TnGBSs, a new family of ICEs associating IS transposition, plasmid replication and conjugation for their spreading (submitted)
4] Guérillot R, Glaser P, (2013) Diversity of bacterial prokaryotic mutator like transposase, association with conjugation conjugative elements and insertion specificity (on preparation).