ISHaha7

  • Family IS200/IS605
  • Group IS1341
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
HE717023 ND Halobacillus halophilus
Halobacillus halophilus DSM 2266
DNA section
IS Length : 1367 bp

Ends


Left end : ATCATTCATCGGCAGAAGACTCCCTCTTCAAGCGCCGTGTCGAAGACCGGTGACAAGGGGGAGATGAATGCCGTCTCCGAAAGGCGAATGTCTTTGAATA II struct. : Yes
Right end : GACGGGGCTAGCCTAATCATTTGAATGAACCGTTAGGTTCGTGCTCTTAGGAATCCCCCAACTTCAAGGAGTGTCAGCGAGTAAGTGGGGGGTAGTTCAA II struct. : Yes

Insertion site


Left flankLE cleavage siteRight flankRE cleavage site
CCTCCCCTTATTTTAAACGCTTCATCAA
ACTTGAATTTTTTTAAACTGAAACTCAA
ATACCGGTTGTTTTAAACTCTAAATCAA
CATATCTCGTTTTTGACCTCTATCTCAA
CCGCCGCTTGTTTTTCAGGCATCATCAA
ATGGGATTTGTTTTTCGTTCATTATCAA
TTGATCTTTTTTTTTTATACCTTATCAA
GCACGAATCCTTTTGCTGGAGTTGTCAA
GTCTCCTAATTTTTTAATTAAGGATCAA

DNA sequence

ATCATTCATCGGCAGAAGACTCCCTCTTCAAGCGCCGTGTCGAAGACCGGTGACAAGGGGGAGATGAATGCCGTCTCCGAAAGGCGAATGTCTTTGAATA
TTCTCCCTTCCGTGATATCCTAAAGCAAGAACGTATGTTCCTGATTGGGAGGTGGAAAGGTTGAAGCATAAAGCGTATAACTTTCGTATCTATCCAAATA
AAACCCAAGAATCGCTCATCGCTAAGACAATCGGATGCGCTCGTTTTGTCTTCAATCATTTCCTCTCGGAATGGAGCAGTGCCTACAGGGAAACCGGAAA
AGGTTTGAGTTACTCTACTTGTTCCAAACAACTACCCAAGCTTAAAAGAGAATTCCTTTGGTTAAAGGAAGTGGATAGCACGGCCATCCAAACTTCGGTC
AAACATCTTTCTGACGCTTATAAACGGTTTTTCAAAAAGCAAAACCAAGCTCCACGGTACAAATCAAAGAAGAACAGAGTCCAGTCCTACACCACAAAAT
GCACAAACGGAAATATTGCCATCCAGGATCACAAAATCAAGCTGCCTAAGCTGGGGCTTGTGAGATTTGCCAAGAGCCGTGAAGTGAAAGGTCGCATCTT
GAGTGCGACGGTTCGCCGCAATCCGACCGGAAAATATTCTGTATCCATTCTTGTTAAAACAGATATTCAGGAACTTCCTAAATCCAACACTTATGTTGGC
ATTGATCTCGGATTGAAAGACTTCGCTGCTCTCTCGAATGGCACCACCTATCAAAACCCACGATACCTACGTAAGACCGAAAAGAAACTAGCAAAAGCTC
AACGTATCCTTTCCCGAAGGGAAAAGGGATCTTCGAATTGGCATAAGCAACGCATTAAAGTAGCTAGACTTCACGAGAAGATAGCGAACTCCCGCAAAGA
TTATCTGCATAAGATTTCTACCGAAATCATCAAAAACCACGATGTGATCGGTGTAGAAAATCTCCAAATTTCCAGTATGCTCAAAAACCGAAAACGATCC
AAATCGATTAGTGATGTGAGCTGGTCGATGTTCACGTCTATGCTCGAATACAAAGCAAAGTGGTACGGGAAGCAGTTGGTAACCGTATCGAAAGTTTTTG
CGAGTTCACAGCTGTGTTCCAAGTGCGGATACCAAAACAGTGAGGTAAAGAATCTCGCTGTTCGAACGTGGGATTGCCCAGATTGCGGCTGCCACCACGA
CCGCGACCTAAATGCGAGCTTCAATATATTGAAGGAGATTCAAAGAATCCTTTACAACCGTCGGTGCGACGGGGCTAGCCTAATCATTTGAATGAACCGT
TAGGTTCGTGCTCTTAGGAATCCCCCAACTTCAAGGAGTGTCAGCGAGTAAGTGGGGGGTAGTTCAA
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
1140 bp379 aa1521291+No
ORF function : Accessory Gene
AG : TnpB

ORF sequence :

MERLKHKAYNFRIYPNKTQESLIAKTIGCARFVFNHFLSEWSSAYRETGKGLSYSTCSKQLPKLKREFLWLKEVDSTAIQTSVKHLSDAYKRFFKKQNQA
PRYKSKKNRVQSYTTKCTNGNIAIQDHKIKLPKLGLVRFAKSREVKGRILSATVRRNPTGKYSVSILVKTDIQELPKSNTYVGIDLGLKDFAALSNGTTY
QNPRYLRKTEKKLAKAQRILSRREKGSSNWHKQRIKVARLHEKIANSRKDYLHKISTEIIKNHDVIGVENLQISSMLKNRKRSKSISDVSWSMFTSMLEY
KAKWYGKQLVTVSKVFASSQLCSKCGYQNSEVKNLAVRTWDCPDCGCHHDRDLNASFNILKEIQRILYNRRCDGASLII

 

Blast result :
Comments
ISHaha7 is 85% (TnpB) aa similar to ISBth17.
References
1] Saum S.H., Pfeiffer F., Palm P., Rampp M., Schuster S.C., Müller V., and Oesterhelt D. (2013) Environ. Microbiol. 15 (5), 1619-1633.