ISAlac1
- Family ISLre2
- Group
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_017167 | ND | Alicyclobacillus acidocaldarius | Alicyclobacillus acidocaldarius subsp. acidocaldarius DSM 446 Alicyclobacillus acidocaldarius subsp. acidocaldarius Tc-4-1 |
DNA section
IS Length : 1608 bp
Ends
IR Length : 17/24
IRL : GAGCGTATAAAGTTTGAGTAGGGGTGTTCTCGGGAGGATTTTCCGGGGAC
IRR : GTCCGTGTCAAGTTGTCGTAGGGGAGGACCTCACCGCTTAGGTTGAGTCA
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| TGGCCGTTTG | TGGGAATTG | TTGAGGATTG | 9 |
| ACGGCTCGCA | TGGCGGGTT | ACCGCGGTTG | 9 |
| GAGTCGACGT | GTAGCGTAC | GCGTGCCTTG | 9 |
| AGTCGAACCG | TGTCCGAAAG | GCTGGCGTGG | 10 |
| GATGTCCCAA | ATGAGAAAT | TTTGTGCTTG | 9 |
| CGTGGCGCTC | GCAGCCGCG | CTCCGGCTTC | 9 |
DNA sequence
GAGCGTATAAAGTTTGAGTAGGGGTGTTCTCGGGAGGATTTTCCGGGGACAAAGAGAAGAGACCTCACTGAGCACAAAAACAACCCGCGTTCGGAGCGCG
GATGAAGAGGTGAGGTCTCTCATGTTGGATATTCGTGATGGCGCGGCGGGTTTCCTGCCGCTGTGGATGAATCTGTTGTGGTTGTCTGAGCATCCGTGCG
ACTTCGCGATTCTTGAAGAGAGGCTCATGAGGTCCTTCGGCGAATTCATGAGGGAAATGGTTCCGCGCTTGCTCGAAGCGTGGGACGAGAAGCTGGCGGC
GGAGCGTGACCGAAATGAGTGGGAACTTATTCAGCGTAAACCACGGACGGTCGTGTCCACCCTGGGAGAGATCACGTATCGTCGGCGTTACTACCGCAAT
CGAAAGACGGGCGAACGTCGCTTCCTGTTGGATGAGGTAGCAGGGTTCGAACCCCGTCGTCGATTGAGCGGGAAGTTAAGAGACCAAGCTGTGGCGTTGG
CTTTAGATGTGTCGTACCGGCGCGCGGCAGAGATTCTACAGACTTGGGTGCCGGACATCAGCGCGATGGCGATTTGGCAAGAAGTGCAAAGGCTCGGAGG
GGAGAAGCGCGTCCGAGCGGAGCTGGAGCGGGAGAAGGTGTTCGAACAAGGAGACGCTCCGGGAGGGAGACGCAAAGCCGAGACACTCTTCGTGGAAGCG
GATGGCGTGTATGTCCGAGGGCGCAGGGAGGAAGATGGCCAGTCACACTTCGAATTGAAGGTGGCCGTGGCGTATGAAGGCAAGCAGGAACAAGGGCGGG
AACGGCGCGCCCTGATGAATCGACAGGTGGTCGCGGGGACTGAGGAAGCTGAACGCTTCTGGGAGGAAGCGGTGTCCTCTTTTGGGCGCGTGTGGGATTG
GGCCAGCGTGAAACGATGTTGGCTCGGCGCGGACGGGGCGGAATGGGCGAAGAAGGGCGTCGGAATGCTCCCTGGGGCTCGATATCGATTGGATCCCTTT
CACCTGCGGCAGGCGATGGTTGCGGCCTTGGGACGGGAGACGCAGGCCTACCGGAAGGTATGGGATGCGGTGAGAACCGAGGAGTGGTCGCGGGTAGAAA
AGGTGCTGACGAAGATGGAGAAGCAAAGCGAAGGGGCGAAGAAGCAGCGTATCTCGAAGCTGAAATCATACATGGAGAAGAATTGGCAAGGCATTGTGGG
CGATGATGATGTGGTGAGTCTGGGTGCAATTGAGGGCCAGGTGTTCCACCACGTGGCGCGACGGATGAAGCGCCATGGGGCACGTTGGAGCAAGCCAGGG
GCGGATGCGTTAGTGCGGCTGATTGCAACGCGCGCCAATCACGAGCCTCTGCGGGCCAACACGCCTTCTCGTTCGGAAATGCAGGCGGAAGTGCCGGTGA
AGACAAAGCCAGTGAACGAGGCGGAAATCGCGAGACGGGTCGAAGAAGCGGCGACTTGGCTGGAAAAACACATGCCAGCATTGGTCGGTCCGCATGCGGA
CCGACCATGGGTGAAGTACGTGCTTCGTGAGCTCGCCAGAGTTTCAGCCACGGTTGCCTGACTCAACCTAAGCGGTGAGGTCCTCCCCTACGACAACTTG
ACACGGAC
GATGAAGAGGTGAGGTCTCTCATGTTGGATATTCGTGATGGCGCGGCGGGTTTCCTGCCGCTGTGGATGAATCTGTTGTGGTTGTCTGAGCATCCGTGCG
ACTTCGCGATTCTTGAAGAGAGGCTCATGAGGTCCTTCGGCGAATTCATGAGGGAAATGGTTCCGCGCTTGCTCGAAGCGTGGGACGAGAAGCTGGCGGC
GGAGCGTGACCGAAATGAGTGGGAACTTATTCAGCGTAAACCACGGACGGTCGTGTCCACCCTGGGAGAGATCACGTATCGTCGGCGTTACTACCGCAAT
CGAAAGACGGGCGAACGTCGCTTCCTGTTGGATGAGGTAGCAGGGTTCGAACCCCGTCGTCGATTGAGCGGGAAGTTAAGAGACCAAGCTGTGGCGTTGG
CTTTAGATGTGTCGTACCGGCGCGCGGCAGAGATTCTACAGACTTGGGTGCCGGACATCAGCGCGATGGCGATTTGGCAAGAAGTGCAAAGGCTCGGAGG
GGAGAAGCGCGTCCGAGCGGAGCTGGAGCGGGAGAAGGTGTTCGAACAAGGAGACGCTCCGGGAGGGAGACGCAAAGCCGAGACACTCTTCGTGGAAGCG
GATGGCGTGTATGTCCGAGGGCGCAGGGAGGAAGATGGCCAGTCACACTTCGAATTGAAGGTGGCCGTGGCGTATGAAGGCAAGCAGGAACAAGGGCGGG
AACGGCGCGCCCTGATGAATCGACAGGTGGTCGCGGGGACTGAGGAAGCTGAACGCTTCTGGGAGGAAGCGGTGTCCTCTTTTGGGCGCGTGTGGGATTG
GGCCAGCGTGAAACGATGTTGGCTCGGCGCGGACGGGGCGGAATGGGCGAAGAAGGGCGTCGGAATGCTCCCTGGGGCTCGATATCGATTGGATCCCTTT
CACCTGCGGCAGGCGATGGTTGCGGCCTTGGGACGGGAGACGCAGGCCTACCGGAAGGTATGGGATGCGGTGAGAACCGAGGAGTGGTCGCGGGTAGAAA
AGGTGCTGACGAAGATGGAGAAGCAAAGCGAAGGGGCGAAGAAGCAGCGTATCTCGAAGCTGAAATCATACATGGAGAAGAATTGGCAAGGCATTGTGGG
CGATGATGATGTGGTGAGTCTGGGTGCAATTGAGGGCCAGGTGTTCCACCACGTGGCGCGACGGATGAAGCGCCATGGGGCACGTTGGAGCAAGCCAGGG
GCGGATGCGTTAGTGCGGCTGATTGCAACGCGCGCCAATCACGAGCCTCTGCGGGCCAACACGCCTTCTCGTTCGGAAATGCAGGCGGAAGTGCCGGTGA
AGACAAAGCCAGTGAACGAGGCGGAAATCGCGAGACGGGTCGAAGAAGCGGCGACTTGGCTGGAAAAACACATGCCAGCATTGGTCGGTCCGCATGCGGA
CCGACCATGGGTGAAGTACGTGCTTCGTGAGCTCGCCAGAGTTTCAGCCACGGTTGCCTGACTCAACCTAAGCGGTGAGGTCCTCCCCTACGACAACTTG
ACACGGAC
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1437 bp | 479 aa | 122 | 1558 | + | No |
Chemistry : DDE
ORF sequence :
MLDIRDGAAGFLPLWMNLLWLSEHPCDFAILEERLMRSFGEFMREMVPRLLEAWDEKLAAERDRNEWELIQRKPRTVVSTLGEITYRRRYYRNRKTGERR
FLLDEVAGFEPRRRLSGKLRDQAVALALDVSYRRAAEILQTWVPDISAMAIWQEVQRLGGEKRVRAELEREKVFEQGDAPGGRRKAETLFVEADGVYVRG
RREEDGQSHFELKVAVAYEGKQEQGRERRALMNRQVVAGTEEAERFWEEAVSSFGRVWDWASVKRCWLGADGAEWAKKGVGMLPGARYRLDPFHLRQAMV
AALGRETQAYRKVWDAVRTEEWSRVEKVLTKMEKQSEGAKKQRISKLKSYMEKNWQGIVGDDDVVSLGAIEGQVFHHVARRMKRHGARWSKPGADALVRL
IATRANHEPLRANTPSRSEMQAEVPVKTKPVNEAEIARRVEEAATWLEKHMPALVGPHADRPWVKYVLRELARVSATVA
FLLDEVAGFEPRRRLSGKLRDQAVALALDVSYRRAAEILQTWVPDISAMAIWQEVQRLGGEKRVRAELEREKVFEQGDAPGGRRKAETLFVEADGVYVRG
RREEDGQSHFELKVAVAYEGKQEQGRERRALMNRQVVAGTEEAERFWEEAVSSFGRVWDWASVKRCWLGADGAEWAKKGVGMLPGARYRLDPFHLRQAMV
AALGRETQAYRKVWDAVRTEEWSRVEKVLTKMEKQSEGAKKQRISKLKSYMEKNWQGIVGDDDVVSLGAIEGQVFHHVARRMKRHGARWSKPGADALVRL
IATRANHEPLRANTPSRSEMQAEVPVKTKPVNEAEIARRVEEAATWLEKHMPALVGPHADRPWVKYVLRELARVSATVA
Blast result :
Comments
ISAlac1 is 58% aa similar to ISTph1.
IS of ISLre2 family preferentially insert in intergenic region, 15 to 17 bp upstream the -35 box of sigmaA promoters. [2][3][4]
IS of ISLre2 family preferentially insert in intergenic region, 15 to 17 bp upstream the -35 box of sigmaA promoters. [2][3][4]
References
1] Romain Guérillot and Philippe Glaser (2013) Direct submission.
2] Brochet M, Da Cunha V, Couvé E, Rusniok C, Trieu-Cuot P, and Glaser P, P.(2009) Mol Microbiol. 71(4):948-59;
3] Guérillot R, Da Cunha V, Sauvage E, Bouchier C, Glaser P, (2013) Modular evolution of TnGBSs, a new family of ICEs associating IS transposition, plasmid replication and conjugation for their spreading (submitted)
4] Guérillot R, Glaser P, (2013) Diversity of bacterial prokaryotic mutator like transposase, association with conjugation conjugative elements and insertion specificity (on preparation).
2] Brochet M, Da Cunha V, Couvé E, Rusniok C, Trieu-Cuot P, and Glaser P, P.(2009) Mol Microbiol. 71(4):948-59;
3] Guérillot R, Da Cunha V, Sauvage E, Bouchier C, Glaser P, (2013) Modular evolution of TnGBSs, a new family of ICEs associating IS transposition, plasmid replication and conjugation for their spreading (submitted)
4] Guérillot R, Glaser P, (2013) Diversity of bacterial prokaryotic mutator like transposase, association with conjugation conjugative elements and insertion specificity (on preparation).