ISKpn20
- Family IS3
- Group IS407
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| JX397875 | ND | Klebsiella pneumoniae | Klebsiella pneumoniae Kpn-1433 plasmid pKP1433 |
DNA section
IS Length : 1196 bp
Ends
IR Length : 12
IRL : GGTAATCCCCCCGAAAAACCAGTGCATGAATAAGTAGAATTTTCTCCTAA
IRR : GGTAATCCCCCCATTTTTAACCGGGCAGATAAGTAGAATCTTATAAGCGC
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| CGCTGGGGTA | GAGG | CAGCCCACTT | 4 |
DNA sequence
GGTAATCCCCCCGAAAAACCAGTGCATGAATAAGTAGAATTTTCTCCTAACCTGAATGCAGGGGGATTTCTATGAAACGATCACGTTTTACTGACAGTCA
GATCATCACCATTCTCAAACAGGCTGAGGCGGGTACGCCGGTTCCTGAACTGTGCCGGGAGCATGGCATCAGCAGTGCCAGTTTTTATAAATGGCGCTCA
AAGTTTGGCGGGATGGATGCTGCACTGATGAGCCGACTGAAAGAGCTTGAAGAAGAAAATCGCCGACTAAAAAAAATGTATGCTGAAGAGCGTCTGAAGG
CCGAAATTATTCAGGAGGCGATGGCAAAAAAGTGGTGAAGCCATCAGACCGGAAGCAGATGGCTCTGCATGCAGTTGAACACCGGGGGATCAGTATCCGT
CTGGCCTGTCAGCTCTTTGTCGTCAGCGAATGTTGCTACCGATACAGGCGCTTACTGAGTCAGGAAAATCAACGGATTGCTGACTGGCTGGTGCGCATCA
CGGACAGCCAGCGAAACTGGGGCTTCGGCCTGTGTTACCTGTATCTGCGTAACGTGAAAGGCTTTGCCTGGAACCACAAGAGAATTTACCGGATTTACTG
CGAACTGTCGCTGAATATGCGGATAAAACCTAAAAAACGGTTGAAACGTGAAAAGCCGGAACCACTGAGGGTCCCCACTGGCAGTAACGAGAGTTGGTCA
ATGGATTTTATGCATGATCAGTTGTCAGATGGTCGCTCCGTTCGTCTGCTGAATATTATTGATGATTTCAATCGCGAGGCCCTGGCAATAGAAGTCGATT
TTTCCCTTCCGGCCAGTCGGGTAGTCAGAACTCTCGAACAACTGATTGAATGGAAAGGGAAACCGGCATCGATTCGATGTGACAACGGACCAGAATATGC
AGGTAACACTCTGATAGTATGGGCTGAACGACAAAATATCATCCTCAATTTTATTCAGCCGGGGAAGCCACAACAGAATGCGTATATTGAGCGTTATAAC
CGGACAGTGCGTTATGACTGGCTGGGGCACTATCTGTTTTATTCGCTGAGTGAGTTACAGGAATACGCCACACAATGGCAATGGTTCTATAATCACGAAC
GACCGAATATGGCACTGAATGGCTTCACACCAATGCAGCATATTCAGCGCTTATAAGATTCTACTTATCTGCCCGGTTAAAAATGGGGGGATTACC
GATCATCACCATTCTCAAACAGGCTGAGGCGGGTACGCCGGTTCCTGAACTGTGCCGGGAGCATGGCATCAGCAGTGCCAGTTTTTATAAATGGCGCTCA
AAGTTTGGCGGGATGGATGCTGCACTGATGAGCCGACTGAAAGAGCTTGAAGAAGAAAATCGCCGACTAAAAAAAATGTATGCTGAAGAGCGTCTGAAGG
CCGAAATTATTCAGGAGGCGATGGCAAAAAAGTGGTGAAGCCATCAGACCGGAAGCAGATGGCTCTGCATGCAGTTGAACACCGGGGGATCAGTATCCGT
CTGGCCTGTCAGCTCTTTGTCGTCAGCGAATGTTGCTACCGATACAGGCGCTTACTGAGTCAGGAAAATCAACGGATTGCTGACTGGCTGGTGCGCATCA
CGGACAGCCAGCGAAACTGGGGCTTCGGCCTGTGTTACCTGTATCTGCGTAACGTGAAAGGCTTTGCCTGGAACCACAAGAGAATTTACCGGATTTACTG
CGAACTGTCGCTGAATATGCGGATAAAACCTAAAAAACGGTTGAAACGTGAAAAGCCGGAACCACTGAGGGTCCCCACTGGCAGTAACGAGAGTTGGTCA
ATGGATTTTATGCATGATCAGTTGTCAGATGGTCGCTCCGTTCGTCTGCTGAATATTATTGATGATTTCAATCGCGAGGCCCTGGCAATAGAAGTCGATT
TTTCCCTTCCGGCCAGTCGGGTAGTCAGAACTCTCGAACAACTGATTGAATGGAAAGGGAAACCGGCATCGATTCGATGTGACAACGGACCAGAATATGC
AGGTAACACTCTGATAGTATGGGCTGAACGACAAAATATCATCCTCAATTTTATTCAGCCGGGGAAGCCACAACAGAATGCGTATATTGAGCGTTATAAC
CGGACAGTGCGTTATGACTGGCTGGGGCACTATCTGTTTTATTCGCTGAGTGAGTTACAGGAATACGCCACACAATGGCAATGGTTCTATAATCACGAAC
GACCGAATATGGCACTGAATGGCTTCACACCAATGCAGCATATTCAGCGCTTATAAGATTCTACTTATCTGCCCGGTTAAAAATGGGGGGATTACC
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 267 bp | 88 aa | 72 | 338 | + | No |
Description : First part of the transposase
ORF sequence :
MKRSRFTDSQIITILKQAEAGTPVPELCREHGISSASFYKWRSKFGGMDAALMSRLKELEEENRRLKKMYAEERLKAEIIQEAMAKKW
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 870 bp | 289 aa | 287 | 1156 | + | No |
Description : Second part of the transposase
ORF sequence :
RASEGRNYSGGDGKKVVKPSDRKQMALHAVEHRGISIRLACQLFVVSECCYRYRRLLSQENQRIADWLVRITDSQRNWGFGLCYLYLRNVKGFAWNHKRI
YRIYCELSLNMRIKPKKRLKREKPEPLRVPTGSNESWSMDFMHDQLSDGRSVRLLNIIDDFNREALAIEVDFSLPASRVVRTLEQLIEWKGKPASIRCDN
GPEYAGNTLIVWAERQNIILNFIQPGKPQQNAYIERYNRTVRYDWLGHYLFYSLSELQEYATQWQWFYNHERPNMALNGFTPMQHIQRL
YRIYCELSLNMRIKPKKRLKREKPEPLRVPTGSNESWSMDFMHDQLSDGRSVRLLNIIDDFNREALAIEVDFSLPASRVVRTLEQLIEWKGKPASIRCDN
GPEYAGNTLIVWAERQNIILNFIQPGKPQQNAYIERYNRTVRYDWLGHYLFYSLSELQEYATQWQWFYNHERPNMALNGFTPMQHIQRL
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1085 bp | 361 aa | 72 | 1156 | + | Yes |
Chemistry : DDE
ORF sequence :
MKRSRFTDSQIITILKQAEAGTPVPELCREHGISSASFYKWRSKFGGMDAALMSRLKELEEENRRLKKMYAEERLKAEIIQEAMAKKVVKPSDRKQMALH
AVEHRGISIRLACQLFVVSECCYRYRRLLSQENQRIADWLVRITDSQRNWGFGLCYLYLRNVKGFAWNHKRIYRIYCELSLNMRIKPKKRLKREKPEPLR
VPTGSNESWSMDFMHDQLSDGRSVRLLNIIDDFNREALAIEVDFSLPASRVVRTLEQLIEWKGKPASIRCDNGPEYAGNTLIVWAERQNIILNFIQPGKP
QQNAYIERYNRTVRYDWLGHYLFYSLSELQEYATQWQWFYNHERPNMALNGFTPMQHIQRL
AVEHRGISIRLACQLFVVSECCYRYRRLLSQENQRIADWLVRITDSQRNWGFGLCYLYLRNVKGFAWNHKRIYRIYCELSLNMRIKPKKRLKREKPEPLR
VPTGSNESWSMDFMHDQLSDGRSVRLLNIIDDFNREALAIEVDFSLPASRVVRTLEQLIEWKGKPASIRCDNGPEYAGNTLIVWAERQNIILNFIQPGKP
QQNAYIERYNRTVRYDWLGHYLFYSLSELQEYATQWQWFYNHERPNMALNGFTPMQHIQRL
Blast result :
Comments
ISKpn20 is 80% aa similar to ISAca1.
The third ORF is a putative ORFAB transposase reconstructed in silico by a possible -1 frameshift.
The third ORF is a putative ORFAB transposase reconstructed in silico by a possible -1 frameshift.
References
1] Papagiannitsis C. (2013) Direct submission to ISfinder
2] Papagiannitsis,C.C., Miriagou,V., Giakkoupi,P., Tzouvelekis,L.S. and Vatopoulos,A.C. (2013) Antimicrob. Agents Chemother. In press
2] Papagiannitsis,C.C., Miriagou,V., Giakkoupi,P., Tzouvelekis,L.S. and Vatopoulos,A.C. (2013) Antimicrob. Agents Chemother. In press