ISNpu14
- Family ISKra4
- Group ISMich2
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_010628 | ND | Nostoc punctiforme | Nostoc punctiforme PCC 73102 Nostoc punctiforme ATCC 29133 |
DNA section
IS Length : 1169 bp
Ends
IR Length : 23/27
IRL : GGGAGCATCCACTTTTGGAAGATATACAAGTTCTGGAAGAATATAACAAC
IRR : GGGAGCATCCAGTTTTGGCAGAAAGACTCAAATAGCAAGTAAACCATTGA
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| ACAACTAACTGG | CATTGAAT | CTTGTCAGTG | 8 |
| TAACCGAAAAGT | ATTGGAAC | TGCCTCTACG | 8 |
DNA sequence
GGGAGCATCCACTTTTGGAAGATATACAAGTTCTGGAAGAATATAACAACAGATAAAACCTGTGGATGACTCACCATGAACCAGGAAAAACAAGAACGCA
TCAAAGCCTGTTTACAAGAATTGTCAACACTGCTGTATGAAGAAGCAGATAAAAGTAAGCTGACAGACCTCGAAAGTATAGAAAAAACAGTTCGGAGTCA
AATATTAGAACTAGTCAGCCCAGAAATAGCCCTTTTTTTATCGAACAAAAAACTGGAACAAAAGTAGGTAAAACCAGGAAGATTAAAAGCTTAGTGGGGG
AACTGAAATTAAAAGCGAAACAGATACACAGACTGGGTTTAAAGCCAAGAAGTCGTTTAAGTCCATTACTTCAAAAGTGCTGTTTGAGGCTATCAGCAAA
TGAATCATACCAAAAAGCAGAAATCGAGATTGAGGCATTAACAGGGGTGAAAGTTGGTCACACGACACAACAAAAACTAGTTCTCGAACAGGACTTTCAG
CTACCATTTGCAAAACAAGCTGTGTCAGAACTGAGTGTGGATGGTGGAAAAGTCCGGCTTACAGGTAAACCCAAAGTAGGCTGTCACTGGCGAGACTATA
AAACCGTTCGTTTGCAAGGGATTTACTATGGTGCGTTTTTTGATGACAACCAATCATTAATCGATTATGTCAATAGCCAATGTTTGGTGAATCCACTTGT
TTGCTTGGGAGATGGTCATGATGGAGTTTGGAATTTAGTTAAAGAGTTTGGAACTGAACAATTTGAACGTTGGGAAATTTCGGATTGGTATCACCTCAAG
GAAAATCTTTATAAAATTGGTGGTTCTCTAAAGCGTCTCAAAGCTGCTGAGAGTTTATTGTGGCAAGGTCAGATAGAGCAAATTCAGGCTTTATTTAATA
ATTGCCGAGGTAAACAAATTAAGAAGTTTATCGCTTATCTCGAAAAACATCGCTCTCGCATTATTAACTACAGCTATTACCAAGCTGAACAACTGTGTTC
TATTGGTTCTGGTTCTGTCGAGTCTGCTATTAAACAGATTGGAGCAAGGATTAAAATTTCCGGCGCACAGTGGAATGTTGAAAGTGTCAACCAAATCCTT
TCCCTTCGTTGTGCTTATCTCAATGGTTTACTTGCTATTTGAGTCTTTCTGCCAAAACTGGATGCTCCC
TCAAAGCCTGTTTACAAGAATTGTCAACACTGCTGTATGAAGAAGCAGATAAAAGTAAGCTGACAGACCTCGAAAGTATAGAAAAAACAGTTCGGAGTCA
AATATTAGAACTAGTCAGCCCAGAAATAGCCCTTTTTTTATCGAACAAAAAACTGGAACAAAAGTAGGTAAAACCAGGAAGATTAAAAGCTTAGTGGGGG
AACTGAAATTAAAAGCGAAACAGATACACAGACTGGGTTTAAAGCCAAGAAGTCGTTTAAGTCCATTACTTCAAAAGTGCTGTTTGAGGCTATCAGCAAA
TGAATCATACCAAAAAGCAGAAATCGAGATTGAGGCATTAACAGGGGTGAAAGTTGGTCACACGACACAACAAAAACTAGTTCTCGAACAGGACTTTCAG
CTACCATTTGCAAAACAAGCTGTGTCAGAACTGAGTGTGGATGGTGGAAAAGTCCGGCTTACAGGTAAACCCAAAGTAGGCTGTCACTGGCGAGACTATA
AAACCGTTCGTTTGCAAGGGATTTACTATGGTGCGTTTTTTGATGACAACCAATCATTAATCGATTATGTCAATAGCCAATGTTTGGTGAATCCACTTGT
TTGCTTGGGAGATGGTCATGATGGAGTTTGGAATTTAGTTAAAGAGTTTGGAACTGAACAATTTGAACGTTGGGAAATTTCGGATTGGTATCACCTCAAG
GAAAATCTTTATAAAATTGGTGGTTCTCTAAAGCGTCTCAAAGCTGCTGAGAGTTTATTGTGGCAAGGTCAGATAGAGCAAATTCAGGCTTTATTTAATA
ATTGCCGAGGTAAACAAATTAAGAAGTTTATCGCTTATCTCGAAAAACATCGCTCTCGCATTATTAACTACAGCTATTACCAAGCTGAACAACTGTGTTC
TATTGGTTCTGGTTCTGTCGAGTCTGCTATTAAACAGATTGGAGCAAGGATTAAAATTTCCGGCGCACAGTGGAATGTTGAAAGTGTCAACCAAATCCTT
TCCCTTCGTTGTGCTTATCTCAATGGTTTACTTGCTATTTGAGTCTTTCTGCCAAAACTGGATGCTCCC
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 192 bp | 63 aa | 76 | 267 | + | No |
Description : First part of the transposase
ORF sequence :
MNQEKQERIKACLQELSTLLYEEADKSKLTDLESIEKTVRSQILELVSPEIALFLSNKKLEQK
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 984 bp | 327 aa | 159 | 1142 | + | No |
Description : Second part of the transposase
ORF sequence :
ADRPRKYRKNSSESNIRTSQPRNSPFFIEQKTGTKVGKTRKIKSLVGELKLKAKQIHRLGLKPRSRLSPLLQKCCLRLSANESYQKAEIEIEALTGVKVG
HTTQQKLVLEQDFQLPFAKQAVSELSVDGGKVRLTGKPKVGCHWRDYKTVRLQGIYYGAFFDDNQSLIDYVNSQCLVNPLVCLGDGHDGVWNLVKEFGTE
QFERWEISDWYHLKENLYKIGGSLKRLKAAESLLWQGQIEQIQALFNNCRGKQIKKFIAYLEKHRSRIINYSYYQAEQLCSIGSGSVESAIKQIGARIKI
SGAQWNVESVNQILSLRCAYLNGLLAI
HTTQQKLVLEQDFQLPFAKQAVSELSVDGGKVRLTGKPKVGCHWRDYKTVRLQGIYYGAFFDDNQSLIDYVNSQCLVNPLVCLGDGHDGVWNLVKEFGTE
QFERWEISDWYHLKENLYKIGGSLKRLKAAESLLWQGQIEQIQALFNNCRGKQIKKFIAYLEKHRSRIINYSYYQAEQLCSIGSGSVESAIKQIGARIKI
SGAQWNVESVNQILSLRCAYLNGLLAI
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1067 bp | 355 aa | 76 | 1142 | + | Yes |
Chemistry : DDE
ORF sequence :
MNQEKQERIKACLQELSTLLYEEADKSKLTDLESIEKTVRSQILELVSPEIALFFIEQKTGTKVGKTRKIKSLVGELKLKAKQIHRLGLKPRSRLSPLLQ
KCCLRLSANESYQKAEIEIEALTGVKVGHTTQQKLVLEQDFQLPFAKQAVSELSVDGGKVRLTGKPKVGCHWRDYKTVRLQGIYYGAFFDDNQSLIDYVN
SQCLVNPLVCLGDGHDGVWNLVKEFGTEQFERWEISDWYHLKENLYKIGGSLKRLKAAESLLWQGQIEQIQALFNNCRGKQIKKFIAYLEKHRSRIINYS
YYQAEQLCSIGSGSVESAIKQIGARIKISGAQWNVESVNQILSLRCAYLNGLLAI
KCCLRLSANESYQKAEIEIEALTGVKVGHTTQQKLVLEQDFQLPFAKQAVSELSVDGGKVRLTGKPKVGCHWRDYKTVRLQGIYYGAFFDDNQSLIDYVN
SQCLVNPLVCLGDGHDGVWNLVKEFGTEQFERWEISDWYHLKENLYKIGGSLKRLKAAESLLWQGQIEQIQALFNNCRGKQIKKFIAYLEKHRSRIINYS
YYQAEQLCSIGSGSVESAIKQIGARIKISGAQWNVESVNQILSLRCAYLNGLLAI
Blast result :
Comments
ISNpu14 is 68% aa similar to ISMae42..
The third ORF is the putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
The third ORF is the putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
References
1] Guérillot R, Glaser P, (2013) Diversity of bacterial prokaryotic mutator like transposase, association with conjugation conjugative elements and insertion specificity (on preparation).
2] Copeland,A., Lucas,S., Lapidus,A., Glavina del Rio,T., Dalin,E., Tice,H., Pitluck,S., Chain,P., Malfatti,S., Shin,M., Vergez,L., Schmutz,J., Larimer,F., Land,M., Hauser,L., Kyrpides,N., Kim,E., Meeks,J.C., Elhai,J., Campbell,E.L., Thiel,T., Longmire,J., Potts,M. and Atlas,R. (2008) Direct submission GenBank.
2] Copeland,A., Lucas,S., Lapidus,A., Glavina del Rio,T., Dalin,E., Tice,H., Pitluck,S., Chain,P., Malfatti,S., Shin,M., Vergez,L., Schmutz,J., Larimer,F., Land,M., Hauser,L., Kyrpides,N., Kim,E., Meeks,J.C., Elhai,J., Campbell,E.L., Thiel,T., Longmire,J., Potts,M. and Atlas,R. (2008) Direct submission GenBank.