ISKra2
- Family ISKra4
- Group ISKra4
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NZ_ADVG01000001.1 | ND | Ktedonobacter racemifer | Ktedonobacter racemifer DSM 44963 |
DNA section
IS Length : 1665 bp
Ends
IR Length : 23/24
IRL : GGGTGAGCGTCATGTTTTTGCAATTGGGCTAGACAAAAGCCCCCTCTTCT
IRR : GGGTGAGCGTCATCTTTTTGCAATGGATTTGGGACGACAAGCCAACACTC
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| CTCCGCGAAG | GGAGGGTAC | CGAGAAATAG | 9 |
| CAAGAGCTAC | TCCCATGGG | GTACGACTCA | 9 |
| CATACCCCAG | TTGGCCTCC | CTGCGTCATC | 9 |
| CTGCCTCCCT | ATGCGCCCG | AGGCCCCTTG | 9 |
| GCACCCACTC | TTTACTCTG | ATATGGGTAG | 9 |
| TGGCTGTGTA | T/GCTGTGTAT | GGGTGTGGCA | 11 |
| CATTTTACTT | GAGGGAATG | CGCCCGCTTG | 9 |
DNA sequence
GGGTGAGCGTCATGTTTTTGCAATTGGGCTAGACAAAAGCCCCCTCTTCTGCGAAGATGTGTTCATTCTCAAGAGGGGAAGGGAAAAACATGAGCGAAGA
GACTGATCAAGGCTGGCAACAACTCAGCGCACAGATTCTCACCGATATCAAAGCCTGGCGGCGAGACCATCCCAAGGTGACCTTACGTGAGATTGAACAG
GAAGTGCATCAGCGCCTGAGTCGACTGGAAGCCCAAGTGATTCAAGATGCAGCTCAAGCAAGTGCGCAACGTGACTGGAGTGGTACCTCAGCCCAAGAGC
GCCCCACCTGTCCCGTGTGTGGCACCCCACTGCAAGCCCGTGGGAAACGACCCCGCCGTCTGCAAGGAGCAGGAGGACAAGAGATCACGCTCAGCAGAAC
GTACGGAACTTGCCCCACGTGTGGGACAGGGCTTTTTCCCCCTCGATGAGGAGTTAGCTCTCCCAAGAGGGAGGAACTTGACATCGTTGCAACACGAGCA
TCTGGTCCATCTTTCGAGCTGGATGCCTTTTGCGCGAGCTGCTGAGATGATGCAACGTCTGCTCGGGGTTCAGGTCAGTGAAGCGAGCGCGCGTCGACAG
AGTGAGCAGGCAGGTGCCTTGGCCCTGGCGGTGCAAACGGTTCTGGCCTCGGAACCTTTCGCCTCGTCACCATCTGTGAGCAAGCCTACCAAGCGCCTGG
CCATCAGCGCCGATGGGGCCTATGTGCCTTTGCTCAAAGGGCAGTGGGCAGAGGTTCGAACGGTGGCTTTTGGAGAAGTCGAACCCTCCACCAGTCCGGC
TGGAGCACAAGAGGTCACGGTCAGTCATCTCTCCTATTTTTCTCGCATGTGTGATGCTCAGACCTTTGCGACCTTGGCTGATGTGGAGATGCGAGAGCGA
GGGGTCCGGCAGGCTCAAGAAATCGGAGCAGTCACTGATGGTGCCGAGTGGTTGCAGGGCTTCCTCGATCTCCACTGTCCCCAAGCGTTGCGCATCTTGG
ATTTTCCTCATGCAGCAGAGCATCTCCATCTGCTCATCCAAGCGCTCCAACAAGCCGGCCTCGTTCTGCCTGACGACCTGCTGGAGCGTGCCTGCCATCG
ACTCAAACATCGAGGTCCCCGTTTGCTCATCTGGCTGCTTGCTCGTTTACCAGCTCAGATCGCCCAACAGGAAGGGGTGCGTGAACAGGTCGGCTATTTT
CTCAAACGCGAAGCCTTGATGCAGTATCCCCAGTTTCAAGCCGCAGGCTGGCCGATTGGGTCAGGGATGGTGGAAAGCGCCAATAAAGTGGTGGTGCAAG
CCCGCCTCAAAGGAGCAGGTATGCATTGGGAGCCCAGTCATGTCAATCCCATGCTCACCTTACGCAACGCCGTCTGTAATGAGCGATGGGATCTCTGCTG
GCAAGAGATTGAGCGGGAGCAACTCCGTCACCGGGAGCAGCGTCGCAACGAGCGAGCGACACCTCGTCTTGAGCAGATGGTCTCTTCCTTGATGCTCTCG
CTCCTTCGCTTTCGTTCCCCTACTCCCAAGCCACTTCCCCCTCCCCCTCGTCCAATGGCACCTGCTGCCACCTTACCTGGTTCCTCACGCCCTTCAACCC
ATCATCCATGGAAACGAGTGTTGGCTTGTCGTCCCAAATCCATTGCAAAAAGATGACGCTCACCC
GACTGATCAAGGCTGGCAACAACTCAGCGCACAGATTCTCACCGATATCAAAGCCTGGCGGCGAGACCATCCCAAGGTGACCTTACGTGAGATTGAACAG
GAAGTGCATCAGCGCCTGAGTCGACTGGAAGCCCAAGTGATTCAAGATGCAGCTCAAGCAAGTGCGCAACGTGACTGGAGTGGTACCTCAGCCCAAGAGC
GCCCCACCTGTCCCGTGTGTGGCACCCCACTGCAAGCCCGTGGGAAACGACCCCGCCGTCTGCAAGGAGCAGGAGGACAAGAGATCACGCTCAGCAGAAC
GTACGGAACTTGCCCCACGTGTGGGACAGGGCTTTTTCCCCCTCGATGAGGAGTTAGCTCTCCCAAGAGGGAGGAACTTGACATCGTTGCAACACGAGCA
TCTGGTCCATCTTTCGAGCTGGATGCCTTTTGCGCGAGCTGCTGAGATGATGCAACGTCTGCTCGGGGTTCAGGTCAGTGAAGCGAGCGCGCGTCGACAG
AGTGAGCAGGCAGGTGCCTTGGCCCTGGCGGTGCAAACGGTTCTGGCCTCGGAACCTTTCGCCTCGTCACCATCTGTGAGCAAGCCTACCAAGCGCCTGG
CCATCAGCGCCGATGGGGCCTATGTGCCTTTGCTCAAAGGGCAGTGGGCAGAGGTTCGAACGGTGGCTTTTGGAGAAGTCGAACCCTCCACCAGTCCGGC
TGGAGCACAAGAGGTCACGGTCAGTCATCTCTCCTATTTTTCTCGCATGTGTGATGCTCAGACCTTTGCGACCTTGGCTGATGTGGAGATGCGAGAGCGA
GGGGTCCGGCAGGCTCAAGAAATCGGAGCAGTCACTGATGGTGCCGAGTGGTTGCAGGGCTTCCTCGATCTCCACTGTCCCCAAGCGTTGCGCATCTTGG
ATTTTCCTCATGCAGCAGAGCATCTCCATCTGCTCATCCAAGCGCTCCAACAAGCCGGCCTCGTTCTGCCTGACGACCTGCTGGAGCGTGCCTGCCATCG
ACTCAAACATCGAGGTCCCCGTTTGCTCATCTGGCTGCTTGCTCGTTTACCAGCTCAGATCGCCCAACAGGAAGGGGTGCGTGAACAGGTCGGCTATTTT
CTCAAACGCGAAGCCTTGATGCAGTATCCCCAGTTTCAAGCCGCAGGCTGGCCGATTGGGTCAGGGATGGTGGAAAGCGCCAATAAAGTGGTGGTGCAAG
CCCGCCTCAAAGGAGCAGGTATGCATTGGGAGCCCAGTCATGTCAATCCCATGCTCACCTTACGCAACGCCGTCTGTAATGAGCGATGGGATCTCTGCTG
GCAAGAGATTGAGCGGGAGCAACTCCGTCACCGGGAGCAGCGTCGCAACGAGCGAGCGACACCTCGTCTTGAGCAGATGGTCTCTTCCTTGATGCTCTCG
CTCCTTCGCTTTCGTTCCCCTACTCCCAAGCCACTTCCCCCTCCCCCTCGTCCAATGGCACCTGCTGCCACCTTACCTGGTTCCTCACGCCCTTCAACCC
ATCATCCATGGAAACGAGTGTTGGCTTGTCGTCCCAAATCCATTGCAAAAAGATGACGCTCACCC
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1410 bp | 469 aa | 247 | 1656 | + | No |
Chemistry : DDE
ORF sequence :
MQLKQVRNVTGVVPQPKSAPPVPCVAPHCKPVGNDPAVCKEQEDKRSRSAERTELAPRVGQGFFPLDEELALPRGRNLTSLQHEHLVHLSSWMPFARAAE
MMQRLLGVQVSEASARRQSEQAGALALAVQTVLASEPFASSPSVSKPTKRLAISADGAYVPLLKGQWAEVRTVAFGEVEPSTSPAGAQEVTVSHLSYFSR
MCDAQTFATLADVEMRERGVRQAQEIGAVTDGAEWLQGFLDLHCPQALRILDFPHAAEHLHLLIQALQQAGLVLPDDLLERACHRLKHRGPRLLIWLLAR
LPAQIAQQEGVREQVGYFLKREALMQYPQFQAAGWPIGSGMVESANKVVVQARLKGAGMHWEPSHVNPMLTLRNAVCNERWDLCWQEIEREQLRHREQRR
NERATPRLEQMVSSLMLSLLRFRSPTPKPLPPPPRPMAPAATLPGSSRPSTHHPWKRVLACRPKSIAKR
MMQRLLGVQVSEASARRQSEQAGALALAVQTVLASEPFASSPSVSKPTKRLAISADGAYVPLLKGQWAEVRTVAFGEVEPSTSPAGAQEVTVSHLSYFSR
MCDAQTFATLADVEMRERGVRQAQEIGAVTDGAEWLQGFLDLHCPQALRILDFPHAAEHLHLLIQALQQAGLVLPDDLLERACHRLKHRGPRLLIWLLAR
LPAQIAQQEGVREQVGYFLKREALMQYPQFQAAGWPIGSGMVESANKVVVQARLKGAGMHWEPSHVNPMLTLRNAVCNERWDLCWQEIEREQLRHREQRR
NERATPRLEQMVSSLMLSLLRFRSPTPKPLPPPPRPMAPAATLPGSSRPSTHHPWKRVLACRPKSIAKR
Blast result :
Comments
ISKra2 is 44% aa similar to ISSiac4.
References
1] Guérillot R, Glaser P, (2013) Diversity of bacterial prokaryotic mutator like transposase, association with conjugation conjugative elements and insertion specificity (on preparation).
2] Lucas,S., Copeland,A., Lapidus,A., Glavina del Rio,T., Dalin,E., Tice,H., Bruce,D., Goodwin,L., Pitluck,S., Kyrpides,N., Mavromatis,K., Ivanova,N., Chertkov,O., Brettin,T., Detter,J.C., Han,C., Land,M., Hauser,L., Markowitz,V., Cheng,J.-F., Hugenholtz,P., Woyke,T., Wu,D., Jando,J., Schneider,S., Sikorski,J., Klenk,H.-P. and Eisen,J.A. (2010) Direct submission GenBank.
2] Lucas,S., Copeland,A., Lapidus,A., Glavina del Rio,T., Dalin,E., Tice,H., Bruce,D., Goodwin,L., Pitluck,S., Kyrpides,N., Mavromatis,K., Ivanova,N., Chertkov,O., Brettin,T., Detter,J.C., Han,C., Land,M., Hauser,L., Markowitz,V., Cheng,J.-F., Hugenholtz,P., Woyke,T., Wu,D., Jando,J., Schneider,S., Sikorski,J., Klenk,H.-P. and Eisen,J.A. (2010) Direct submission GenBank.