ISNpu15
- Family ISKra4
- Group ISMich2
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_010628 | ND | Nostoc punctiforme | Nostoc punctiforme PCC 73102 Nostoc punctiforme ATCC 29133 |
DNA section
IS Length : 1218 bp
Ends
IR Length : 20/21
IRL : GGGTGCATCCCAGTTTTTATTATTCCAAAACAAATAAATAACAGGGAAGG
IRR : GGGAGCATCCCAGTTTTTATTTAGTGAGCAAAGATTAATCCATTAAGATA
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| AGACCACCGA | TTCACTAAT | CCCCTTGTCG | 9 |
| TCCAAAGAGC | ATTAATAAG | AGCCTGAGAT | 9 |
| AGTTAAAGAA | ACGCTAGAG | TTTGTCAAAT | 9 |
| TAGAACTATT | GTAGAGACT | TAGCACTGCTA | 9 |
| AAGATAAATTAGTTTATTAT | GATACAGCGTAAGTATATTT | 0 | |
| GAAATGAGCTTGTATACAAA | ATGAAGTCTTCAGTCGAGAG | 0 | |
| TGATTGCACTTTTGTCAAGA | TGTCATAGGCGACACTAAAA | 0 | |
| CTAGAACTATTGTAGAGACT | AATTAATATTTGCTGAACGC | 0 |
DNA sequence
GGGTGCATCCCAGTTTTTATTATTCCAAAACAAATAAATAACAGGGAAGGATTGTTACAGAAAACGGAAGTCAGGAAGTAAGGGGATATCAGGAAGGGAT
AATAAAGTCATCAAGACTAGGTGTATCAATGACTCCTGAAGAAGAACAACAGATAAAAGAATATTCTCGTGCAATAGCAAAGATACTGTACAAAAGTACT
ACGGGTGAGCAACTCACAAGTTTGGCAAAAATAGAAGAAGTAGTACGCTCTCAAATGCGGAAGCATGTAATGCCAGAAGTAGGGTTTTTTTATCGAAAAT
GCCACAGGAGAAAGCAGAGGATACAAACGAAAAATAAAAAGTATTATTGGAGAACTGCCAATTACAAACTCTCAAGCACAAAAGCTAGAAATTAGACCCC
ATAATCAATTGAGTCCATATTTAGAAGCTTGTTGCTTACGAATCAGCGCGTCGGTATCGTATCAACGTGCGGCAGAAGATATTGAATATTTAACTGGTGT
AGAAGTATCAAAAAGTGTGCAGCAACGATTAGTGCATCGTCAAAATTTTGAATTACCGCAAGTAGAATCAACTGTGAAAGAATTGAGTGTGGATGGAGGA
AATATACGCATTCGTACAATCAAGGGACAAGTCTGTGATTGGAAGGGTTATAAAGCTACCTGCTTACATGAAAAACAAGCTATTGCTGCCTCATTTCAAG
AAAATAGTCTTGTAATTGATTGGGTCAAAAGCCAATCAATTGCTCCTATCTTGACCTGTCTTGGCGATGGCCATGACGGTATTTGGAATATTGTCCGCGA
TTTTGCTCCCGAACACCAGCGTCGGGAAGTACTTGATTGGTTTCATCTGATGGAAAACCTACACAAGATTGGCGGTTCTAATCAACGGCTCAATCAGGCT
AAAATCCTTCTCTGGCAAGGAAAAGTTGATGATGCTATCGCTGTCTTTGCTGACTGTCAGCTAAAACAAGCTTTTAATTTCTGTACTTATCTTGAGAAAC
ATCGACACCGGATTGTCAATTACCAATATTATCAAGCAGAACAAATCTGTTCCATTGGTTCTGGTACTATTGAGTCTACTGTCAAACAGATTGACCGTCG
AACCAAAATTTCTGGCGCACAATGGAAATCTGATAACGTTCCTCAAGTCTTAGCTCAACGCCAGAGCTTATCTTAATGGATTAATCTTTGCTCACTAAAT
AAAAACTGGGATGCTCCC
AATAAAGTCATCAAGACTAGGTGTATCAATGACTCCTGAAGAAGAACAACAGATAAAAGAATATTCTCGTGCAATAGCAAAGATACTGTACAAAAGTACT
ACGGGTGAGCAACTCACAAGTTTGGCAAAAATAGAAGAAGTAGTACGCTCTCAAATGCGGAAGCATGTAATGCCAGAAGTAGGGTTTTTTTATCGAAAAT
GCCACAGGAGAAAGCAGAGGATACAAACGAAAAATAAAAAGTATTATTGGAGAACTGCCAATTACAAACTCTCAAGCACAAAAGCTAGAAATTAGACCCC
ATAATCAATTGAGTCCATATTTAGAAGCTTGTTGCTTACGAATCAGCGCGTCGGTATCGTATCAACGTGCGGCAGAAGATATTGAATATTTAACTGGTGT
AGAAGTATCAAAAAGTGTGCAGCAACGATTAGTGCATCGTCAAAATTTTGAATTACCGCAAGTAGAATCAACTGTGAAAGAATTGAGTGTGGATGGAGGA
AATATACGCATTCGTACAATCAAGGGACAAGTCTGTGATTGGAAGGGTTATAAAGCTACCTGCTTACATGAAAAACAAGCTATTGCTGCCTCATTTCAAG
AAAATAGTCTTGTAATTGATTGGGTCAAAAGCCAATCAATTGCTCCTATCTTGACCTGTCTTGGCGATGGCCATGACGGTATTTGGAATATTGTCCGCGA
TTTTGCTCCCGAACACCAGCGTCGGGAAGTACTTGATTGGTTTCATCTGATGGAAAACCTACACAAGATTGGCGGTTCTAATCAACGGCTCAATCAGGCT
AAAATCCTTCTCTGGCAAGGAAAAGTTGATGATGCTATCGCTGTCTTTGCTGACTGTCAGCTAAAACAAGCTTTTAATTTCTGTACTTATCTTGAGAAAC
ATCGACACCGGATTGTCAATTACCAATATTATCAAGCAGAACAAATCTGTTCCATTGGTTCTGGTACTATTGAGTCTACTGTCAAACAGATTGACCGTCG
AACCAAAATTTCTGGCGCACAATGGAAATCTGATAACGTTCCTCAAGTCTTAGCTCAACGCCAGAGCTTATCTTAATGGATTAATCTTTGCTCACTAAAT
AAAAACTGGGATGCTCCC
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 267 bp | 88 aa | 129 | 395 | + | No |
Description : First part of the transposase
ORF sequence :
MTPEEEQQIKEYSRAIAKILYKSTTGEQLTSLAKIEEVVRSQMRKHVMPEVGFFYRKCHRRKQRIQTKNKKYYWRTANYKLSSTKARN
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 884 bp | 297 aa | 293 | 1176 | + | No |
Description : Second part of the transposase
ORF sequence :
GFFIENATGESRGYKRKIKSIIGELPITNSQAQKLEIRPHNQLSPYLEACCLRISASVSYQRAAEDIEYLTGVEVSKSVQQRLVHRQNFELPQVESTVKE
LSVDGGNIRIRTIKGQVCDWKGYKATCLHEKQAIAASFQENSLVIDWVKSQSIAPILTCLGDGHDGIWNIVRDFAPEHQRREVLDWFHLMENLHKIGGSN
QRLNQAKILLWQGKVDDAIAVFADCQLKQAFNFCTYLEKHRHRIVNYQYYQAEQICSIGSGTIESTVKQIDRRTKISGAQWKSDNVPQVLAQRQSLS
LSVDGGNIRIRTIKGQVCDWKGYKATCLHEKQAIAASFQENSLVIDWVKSQSIAPILTCLGDGHDGIWNIVRDFAPEHQRREVLDWFHLMENLHKIGGSN
QRLNQAKILLWQGKVDDAIAVFADCQLKQAFNFCTYLEKHRHRIVNYQYYQAEQICSIGSGTIESTVKQIDRRTKISGAQWKSDNVPQVLAQRQSLS
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1048 bp | 348 aa | 129 | 1176 | + | Yes |
Chemistry : DDE
ORF sequence :
MTPEEEQQIKEYSRAIAKILYKSTTGEQLTSLAKIEEVVRSQMRKHVMPEVGFFIENATGESRGYKRKIKSIIGELPITNSQAQKLEIRPHNQLSPYLEA
CCLRISASVSYQRAAEDIEYLTGVEVSKSVQQRLVHRQNFELPQVESTVKELSVDGGNIRIRTIKGQVCDWKGYKATCLHEKQAIAASFQENSLVIDWVK
SQSIAPILTCLGDGHDGIWNIVRDFAPEHQRREVLDWFHLMENLHKIGGSNQRLNQAKILLWQGKVDDAIAVFADCQLKQAFNFCTYLEKHRHRIVNYQY
YQAEQICSIGSGTIESTVKQIDRRTKISGAQWKSDNVPQVLAQRQSLS
CCLRISASVSYQRAAEDIEYLTGVEVSKSVQQRLVHRQNFELPQVESTVKELSVDGGNIRIRTIKGQVCDWKGYKATCLHEKQAIAASFQENSLVIDWVK
SQSIAPILTCLGDGHDGIWNIVRDFAPEHQRREVLDWFHLMENLHKIGGSNQRLNQAKILLWQGKVDDAIAVFADCQLKQAFNFCTYLEKHRHRIVNYQY
YQAEQICSIGSGTIESTVKQIDRRTKISGAQWKSDNVPQVLAQRQSLS
Blast result :
Comments
The third ORf is the putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
ISNpu15 is 70% aa similar to ISMae42.
ISNpu15 is 70% aa similar to ISMae42.
References
1] Guérillot R, Glaser P, (2013) Diversity of bacterial prokaryotic mutator like transposase, association with conjugation conjugative elements and insertion specificity (on preparation).
2] Copeland,A., Lucas,S., Lapidus,A., Glavina del Rio,T., Dalin,E., Tice,H., Pitluck,S., Chain,P., Malfatti,S., Shin,M., Vergez,L., Schmutz,J., Larimer,F., Land,M., Hauser,L., Kyrpides,N., Kim,E., Meeks,J.C., Elhai,J., Campbell,E.L., Thiel,T., Longmire,J., Potts,M. and Atlas,R. (2008) Direct submission GenBank.
2] Copeland,A., Lucas,S., Lapidus,A., Glavina del Rio,T., Dalin,E., Tice,H., Pitluck,S., Chain,P., Malfatti,S., Shin,M., Vergez,L., Schmutz,J., Larimer,F., Land,M., Hauser,L., Kyrpides,N., Kim,E., Meeks,J.C., Elhai,J., Campbell,E.L., Thiel,T., Longmire,J., Potts,M. and Atlas,R. (2008) Direct submission GenBank.