ISNpu16
- Family ISKra4
- Group ISMich2
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_010628 | ND | Nostoc punctiforme | Nostoc punctiforme PCC 73102 |
DNA section
IS Length : 1173 bp
Ends
IR Length : 20/22
IRL : GGGAGCATCCAGTTTTGGAAGATTTACCATTTCTGAGAAAATGTAGAAGG
IRR : GGGAGCATCCACTTTTGGCAGAAACACTCAAATAGCAAGTAAACCATTAA
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| GACACTGGCG | ATCGCGATC | GCTGCTACCC | 9 |
DNA sequence
GGGAGCATCCAGTTTTGGAAGATTTACCATTTCTGAGAAAATGTAGAAGGAAATAACGCTGTGCGATCGCTGACTATGAGCCAAGAGAAACAAGAACGTA
TCAAAGCCTGCTTAGAAGAACTGGCAACATTACTGTACTCGGAAGCTGATAAGAGTCAGTTAATAGACCTCGAAGGTATAGAAAAAACAGTTCGTAGCCA
AATATTGGAATATGTCGGTCCAGAAATAGCCCTTTTTTTATCGAACAAACAACCAAAACAAAAGTAGGTAAAACCAGGAAAGTCAAAAGCCTGGTAGGAG
AATTAAAATTAAAAGCCAAACAGTTACAGAAACTGGGTTTGAAGCCAAGAAGCCGTTTAAGTCCATTACTTCAAAAGTGCTGTTTGCGACTATCAGCAAA
TGTTTCATACCAAAAAGCAGAAATCGAAATTGAGGCATTAACAGGAGTGAAAGTTGGTCACACGACGCAACAAAAACTAGTTCTCGAACAAGATTTTCAA
CTGCCCGTAGCATTACAAGTCGTGTCAGAAGTCAATGTAGATGGAGGAAAAGTCCGACTTAGAGGTAAACCGAAAGCAGGCTGTCACTGGCGAGACTATA
AAACCGTTCGTCTACAAGGGATTTACTATGGTGCGTTTTTTGATGACAACCAATCATTAGTTGATTATGTCAATATTCAGCCTCTGGTTAACCCGTTAGT
CTGCTTAGGAGATGGTCATGATGGTGTGTGGAATCTTGTCAGAGAATTTGGTAAAAGTGAGAATTTTGAGCGTTGGGAAATTTTAGACTGGTATCACCTC
AAAGAAAATCTCTATAAAATTGGTGGTTCTTTAAAACGGCTTAAAGCTGCTGAAAGTTTTTTGTGGCAAGGTCAAATAGATAATACTCAGGCTTTGTTTA
TTCATTGCCGAGGTAAACAGGTGAAAAATTTCATTACTTATCTCAACAAACATCGTTCTCGTATTGTCAACTACAGCTATTACCAGGCTGAAGAACTCTG
TTCTATCGGTTCTGGTGCAGTCGAGTCGGCAATTAAACAGATTGGAGCCAGGATTAAAATTTCTGGTGCACAGTGGAATGTTGAGAGTGTCAACCAAATT
CTCTCTGAACGCTTAAGCTTATCTTAATGGTTTACTTGCTATTTGAGTGTTTCTGCCAAAAGTGGATGCTCCC
TCAAAGCCTGCTTAGAAGAACTGGCAACATTACTGTACTCGGAAGCTGATAAGAGTCAGTTAATAGACCTCGAAGGTATAGAAAAAACAGTTCGTAGCCA
AATATTGGAATATGTCGGTCCAGAAATAGCCCTTTTTTTATCGAACAAACAACCAAAACAAAAGTAGGTAAAACCAGGAAAGTCAAAAGCCTGGTAGGAG
AATTAAAATTAAAAGCCAAACAGTTACAGAAACTGGGTTTGAAGCCAAGAAGCCGTTTAAGTCCATTACTTCAAAAGTGCTGTTTGCGACTATCAGCAAA
TGTTTCATACCAAAAAGCAGAAATCGAAATTGAGGCATTAACAGGAGTGAAAGTTGGTCACACGACGCAACAAAAACTAGTTCTCGAACAAGATTTTCAA
CTGCCCGTAGCATTACAAGTCGTGTCAGAAGTCAATGTAGATGGAGGAAAAGTCCGACTTAGAGGTAAACCGAAAGCAGGCTGTCACTGGCGAGACTATA
AAACCGTTCGTCTACAAGGGATTTACTATGGTGCGTTTTTTGATGACAACCAATCATTAGTTGATTATGTCAATATTCAGCCTCTGGTTAACCCGTTAGT
CTGCTTAGGAGATGGTCATGATGGTGTGTGGAATCTTGTCAGAGAATTTGGTAAAAGTGAGAATTTTGAGCGTTGGGAAATTTTAGACTGGTATCACCTC
AAAGAAAATCTCTATAAAATTGGTGGTTCTTTAAAACGGCTTAAAGCTGCTGAAAGTTTTTTGTGGCAAGGTCAAATAGATAATACTCAGGCTTTGTTTA
TTCATTGCCGAGGTAAACAGGTGAAAAATTTCATTACTTATCTCAACAAACATCGTTCTCGTATTGTCAACTACAGCTATTACCAGGCTGAAGAACTCTG
TTCTATCGGTTCTGGTGCAGTCGAGTCGGCAATTAAACAGATTGGAGCCAGGATTAAAATTTCTGGTGCACAGTGGAATGTTGAGAGTGTCAACCAAATT
CTCTCTGAACGCTTAAGCTTATCTTAATGGTTTACTTGCTATTTGAGTGTTTCTGCCAAAAGTGGATGCTCCC
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 192 bp | 63 aa | 76 | 267 | + | No |
Description : First part of the transposase
ORF sequence :
MSQEKQERIKACLEELATLLYSEADKSQLIDLEGIEKTVRSQILEYVGPEIALFLSNKQPKQK
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 930 bp | 309 aa | 198 | 1127 | + | No |
Description : Second part of the transposase
ORF sequence :
PNIGICRSRNSPFFIEQTTKTKVGKTRKVKSLVGELKLKAKQLQKLGLKPRSRLSPLLQKCCLRLSANVSYQKAEIEIEALTGVKVGHTTQQKLVLEQDF
QLPVALQVVSEVNVDGGKVRLRGKPKAGCHWRDYKTVRLQGIYYGAFFDDNQSLVDYVNIQPLVNPLVCLGDGHDGVWNLVREFGKSENFERWEILDWYH
LKENLYKIGGSLKRLKAAESFLWQGQIDNTQALFIHCRGKQVKNFITYLNKHRSRIVNYSYYQAEELCSIGSGAVESAIKQIGARIKISGAQWNVESVNQ
ILSERLSLS
QLPVALQVVSEVNVDGGKVRLRGKPKAGCHWRDYKTVRLQGIYYGAFFDDNQSLVDYVNIQPLVNPLVCLGDGHDGVWNLVREFGKSENFERWEILDWYH
LKENLYKIGGSLKRLKAAESFLWQGQIDNTQALFIHCRGKQVKNFITYLNKHRSRIVNYSYYQAEELCSIGSGAVESAIKQIGARIKISGAQWNVESVNQ
ILSERLSLS
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1052 bp | 350 aa | 76 | 1127 | + | Yes |
Chemistry : DDE
ORF sequence :
MSQEKQERIKACLEELATLLYSEADKSQLIDLEGIEKTVRSQILEYVGPEIALFFIEQTTKTKVGKTRKVKSLVGELKLKAKQLQKLGLKPRSRLSPLLQ
KCCLRLSANVSYQKAEIEIEALTGVKVGHTTQQKLVLEQDFQLPVALQVVSEVNVDGGKVRLRGKPKAGCHWRDYKTVRLQGIYYGAFFDDNQSLVDYVN
IQPLVNPLVCLGDGHDGVWNLVREFGKSENFERWEILDWYHLKENLYKIGGSLKRLKAAESFLWQGQIDNTQALFIHCRGKQVKNFITYLNKHRSRIVNY
SYYQAEELCSIGSGAVESAIKQIGARIKISGAQWNVESVNQILSERLSLS
KCCLRLSANVSYQKAEIEIEALTGVKVGHTTQQKLVLEQDFQLPVALQVVSEVNVDGGKVRLRGKPKAGCHWRDYKTVRLQGIYYGAFFDDNQSLVDYVN
IQPLVNPLVCLGDGHDGVWNLVREFGKSENFERWEILDWYHLKENLYKIGGSLKRLKAAESFLWQGQIDNTQALFIHCRGKQVKNFITYLNKHRSRIVNY
SYYQAEELCSIGSGAVESAIKQIGARIKISGAQWNVESVNQILSERLSLS
Blast result :
Comments
ISNpu16 is 92% aa similar to ISNpu14.
The third ORF is the putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
The third ORF is the putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
References
1] ISfinder annotation (2013)
2] Copeland,A., Lucas,S., Lapidus,A., Glavina del Rio,T., Dalin,E., Tice,H., Pitluck,S., Chain,P., Malfatti,S., Shin,M., Vergez,L., Schmutz,J., Larimer,F., Land,M., Hauser,L., Kyrpides,N., Kim,E., Meeks,J.C., Elhai,J., Campbell,E.L., Thiel,T., Longmire,J., Potts,M. and Atlas,R. (2008) Direct submission GenBank.
2] Copeland,A., Lucas,S., Lapidus,A., Glavina del Rio,T., Dalin,E., Tice,H., Pitluck,S., Chain,P., Malfatti,S., Shin,M., Vergez,L., Schmutz,J., Larimer,F., Land,M., Hauser,L., Kyrpides,N., Kim,E., Meeks,J.C., Elhai,J., Campbell,E.L., Thiel,T., Longmire,J., Potts,M. and Atlas,R. (2008) Direct submission GenBank.