ISKra4
- Family ISKra4
- Group ISKra4
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NZ_ADVG01000001 | ND | Ktedonobacter racemifer | Ktedonobacter racemifer DSM 44963 |
DNA section
IS Length : 1657 bp
Ends
IR Length : 22
IRL : GGGTGCGGTTCATTTTTTTGCAAACTCAGCGATTGGGACGAAGAGGAAGG
IRR : GGGTGCGGTTCATTTTTTTGCATAGCGTCCATCTTGGATAGCTCGCTGGG
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| AGTCCTGACC | CCATTCCTG | GGGTGCGGTT | 9 |
| AGTCCTGACC | CCATTCCTG | GGGTGCGGTT | 9 |
| CAAGAAGGAA | AGATTCATT | GTATTGCCTG | 9 |
| AGGCCTGGTA | GGCTGCGAA | GGCTTCCTTG | 9 |
DNA sequence
GGGTGCGGTTCATTTTTTTGCAAACTCAGCGATTGGGACGAAGAGGAAGGGGAAATGATAAGATAAAAAAACAGGAAGAAGCCAGCAAGGAGGTTGCCGA
TGGAAAAATCCTCACTCGCCGAAGTCTGGAGACTGCTCAGCGAGAGCGTCTTTGAAGAAATGGAAGCGTGGCGCAAAGAGCACCCAACAGCGACATTCCA
AGAGATCGAAGACGAGCTGGATGCTCGGCTCTCAGGCATGCGGGCCCAGATGTTAGCTGACCTAGCTCAACAGAGTCCCAAACGGGATTGGAGTGGACAA
GAAGCAGAGAAGCGTCCCCGTTGCCCGCAGTGTGGAGCGGACTTACAGGCGCGAGGTAAGCACGAGCGAAGATTGGCAACGCAAGGGGGCCAAGAGGTCA
AACTCAGCCGACGGTATGGAACGTGCCCGCAGTGTGGAAGCGGGCTTTTTCCCCCTGGATGAAGAACTGAATCTGCCCTCGAGTGGACTGACGCCTCATG
CCCACCAGGGATTGGTCTTGTTGGGAAGCATGGTGCCATATGCCTGCGCAGCCACACACCTGGAAACCCTTTTGAAAGTCCAGGTCAGTAAGAGCACGGT
GCGCCGACTCACTGAACAGGCAGGAGCATGCTTGCAACAGTGGCAGGATCAATACGCTTGCCCAGGGCAAGGGAGTGAGCAGCCCAAACAGGCAGCGCCG
CGGCAGGCGATGGCAACAGATGGGGTGTTGATCCCCGTTTTGCCAAACGAGTGGGCGGAGGTCAAAATGGTCACAATTGGCGAGGTATGCCAAACGAAGT
CAGGTGAGCCGCATTGCGAGAAGCTGAGTTATTTTGCCCGTCTGGCTGATGCCGAAACCTTTGGAGATCTGGCGTCCTTCGAGATCCAGCGGCGACGAGT
CGATCAAGCCAGAGAGGTAGCCGCCATCAACGATGGAGCAGAATGGATTCAGGGATTTGTGCAAGGCCATCGTGCCGATGCAGTGCGTATTCTGGATTTT
GCGCATGCCGCTCAATACATCAGTGAGATCGCAACCCTGGCCCAAAACGCCGGAGTGCCATTGCCGCCGACATGGGTAAGCGAGCAGTGGCATAAGCTCA
AGGAGGATGGACCGACAAGCGTACTCAAGGAAGTGCGGCGGCTGAGCGCATCAACTCCAGGTGACGAGATGCTCGAGAAGGTGGCCTATCTGCACAAACG
GGAAGGGCAGATGCAGTATCAGCAGTATCGGGCAGGTGGCTGGCCTATTGGATCAGGGATCGCGGAAAGCGGCAACAAACTGGTGGTGCAAGCTCGCCTC
AAAGGAGCAGGGATGCATTGGGCACGTCCTCAGGTGAATGCGATGTTGGCCCTGCGAACCACCTTGTGCAGTAACCGATGGACCCAGGACTGGCATGTGG
TCAGGGCGAGTTGGCACACCAGCCGCCTCCAACGTTTTCGCGCACGCAGTCAAGCGGCTTTGGCCAAAGCCTCTCTTCGCTTGCAGCAAACTTTCTGGCG
CTTGCCTTTGCCTCTTATCCTGGCGTGTTTCCCCTCCCCTCCCCCAACCTCAATGGCTCCCAAAGGACGGACCGAGGGACAGAGGCGTTGGGGACGGCAG
ACCTTTTCCCAGCGAGCTATCCAAGATGGACGCTATGCAAAAAAATGAACCGCACCC
TGGAAAAATCCTCACTCGCCGAAGTCTGGAGACTGCTCAGCGAGAGCGTCTTTGAAGAAATGGAAGCGTGGCGCAAAGAGCACCCAACAGCGACATTCCA
AGAGATCGAAGACGAGCTGGATGCTCGGCTCTCAGGCATGCGGGCCCAGATGTTAGCTGACCTAGCTCAACAGAGTCCCAAACGGGATTGGAGTGGACAA
GAAGCAGAGAAGCGTCCCCGTTGCCCGCAGTGTGGAGCGGACTTACAGGCGCGAGGTAAGCACGAGCGAAGATTGGCAACGCAAGGGGGCCAAGAGGTCA
AACTCAGCCGACGGTATGGAACGTGCCCGCAGTGTGGAAGCGGGCTTTTTCCCCCTGGATGAAGAACTGAATCTGCCCTCGAGTGGACTGACGCCTCATG
CCCACCAGGGATTGGTCTTGTTGGGAAGCATGGTGCCATATGCCTGCGCAGCCACACACCTGGAAACCCTTTTGAAAGTCCAGGTCAGTAAGAGCACGGT
GCGCCGACTCACTGAACAGGCAGGAGCATGCTTGCAACAGTGGCAGGATCAATACGCTTGCCCAGGGCAAGGGAGTGAGCAGCCCAAACAGGCAGCGCCG
CGGCAGGCGATGGCAACAGATGGGGTGTTGATCCCCGTTTTGCCAAACGAGTGGGCGGAGGTCAAAATGGTCACAATTGGCGAGGTATGCCAAACGAAGT
CAGGTGAGCCGCATTGCGAGAAGCTGAGTTATTTTGCCCGTCTGGCTGATGCCGAAACCTTTGGAGATCTGGCGTCCTTCGAGATCCAGCGGCGACGAGT
CGATCAAGCCAGAGAGGTAGCCGCCATCAACGATGGAGCAGAATGGATTCAGGGATTTGTGCAAGGCCATCGTGCCGATGCAGTGCGTATTCTGGATTTT
GCGCATGCCGCTCAATACATCAGTGAGATCGCAACCCTGGCCCAAAACGCCGGAGTGCCATTGCCGCCGACATGGGTAAGCGAGCAGTGGCATAAGCTCA
AGGAGGATGGACCGACAAGCGTACTCAAGGAAGTGCGGCGGCTGAGCGCATCAACTCCAGGTGACGAGATGCTCGAGAAGGTGGCCTATCTGCACAAACG
GGAAGGGCAGATGCAGTATCAGCAGTATCGGGCAGGTGGCTGGCCTATTGGATCAGGGATCGCGGAAAGCGGCAACAAACTGGTGGTGCAAGCTCGCCTC
AAAGGAGCAGGGATGCATTGGGCACGTCCTCAGGTGAATGCGATGTTGGCCCTGCGAACCACCTTGTGCAGTAACCGATGGACCCAGGACTGGCATGTGG
TCAGGGCGAGTTGGCACACCAGCCGCCTCCAACGTTTTCGCGCACGCAGTCAAGCGGCTTTGGCCAAAGCCTCTCTTCGCTTGCAGCAAACTTTCTGGCG
CTTGCCTTTGCCTCTTATCCTGGCGTGTTTCCCCTCCCCTCCCCCAACCTCAATGGCTCCCAAAGGACGGACCGAGGGACAGAGGCGTTGGGGACGGCAG
ACCTTTTCCCAGCGAGCTATCCAAGATGGACGCTATGCAAAAAAATGAACCGCACCC
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 363 bp | 120 aa | 100 | 462 | + | No |
Description : First part of the transposase
ORF sequence :
MEKSSLAEVWRLLSESVFEEMEAWRKEHPTATFQEIEDELDARLSGMRAQMQLADLAQQSPKRDWSGEAEKRPRCPQCGADLQARGKHERRLATQGGQEV
KLSRRYGTCPQCGSGLFPPG
KLSRRYGTCPQCGSGLFPPG
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1383 bp | 460 aa | 266 | 1648 | + | No |
Description : Second part of the transposase
ORF sequence :
LNRVPNGIGVDKKQRSVPVARSVERTYRREVSTSEDWQRKGAKRSNSADGMERARSVEAGFFPLDEELNLPSSGLTPHAHQGLVLLGSMVPYACAATHLE
TLLKVQVSKSTVRRLTEQAGACLQQWQDQYACPGQGSEQPKQAAPRQAMATDGVLIPVLPNEWAEVKMVTIGEVCQTKSGEPHCEKLSYFARLADAETFG
DLASFEIQRRRVDQAREVAAINDGAEWIQGFVQGHRADAVRILDFAHAAQYISEIATLAQNAGVPLPPTWVSEQWHKLKEDGPTSVLKEVRRLSASTPGD
EMLEKVAYLHKREGQMQYQQYRAGGWPIGSGIAESGNKLVVQARLKGAGMHWARPQVNAMLALRTTLCSNRWTQDWHVVRASWHTSRLQRFRARSQAALA
KASLRLQQTFWRLPLPLILACFPSPPPTSMAPKGRTEGQRRWGRQTFSQRAIQDGRYAKK
TLLKVQVSKSTVRRLTEQAGACLQQWQDQYACPGQGSEQPKQAAPRQAMATDGVLIPVLPNEWAEVKMVTIGEVCQTKSGEPHCEKLSYFARLADAETFG
DLASFEIQRRRVDQAREVAAINDGAEWIQGFVQGHRADAVRILDFAHAAQYISEIATLAQNAGVPLPPTWVSEQWHKLKEDGPTSVLKEVRRLSASTPGD
EMLEKVAYLHKREGQMQYQQYRAGGWPIGSGIAESGNKLVVQARLKGAGMHWARPQVNAMLALRTTLCSNRWTQDWHVVRASWHTSRLQRFRARSQAALA
KASLRLQQTFWRLPLPLILACFPSPPPTSMAPKGRTEGQRRWGRQTFSQRAIQDGRYAKK
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1549 bp | 515 aa | 100 | 1648 | + | Yes |
Chemistry : DDE
ORF sequence :
MEKSSLAEVWRLLSESVFEEMEAWRKEHPTATFQEIEDELDARLSGMRAQMLADLAQQSPKRDWSGQEAEKRPRCPQCGADLQARGKHERRLATQGGQEV
KLSRRYGTCPQCGSGLFPLDEELNLPSSGLTPHAHQGLVLLGSMVPYACAATHLETLLKVQVSKSTVRRLTEQAGACLQQWQDQYACPGQGSEQPKQAAP
RQAMATDGVLIPVLPNEWAEVKMVTIGEVCQTKSGEPHCEKLSYFARLADAETFGDLASFEIQRRRVDQAREVAAINDGAEWIQGFVQGHRADAVRILDF
AHAAQYISEIATLAQNAGVPLPPTWVSEQWHKLKEDGPTSVLKEVRRLSASTPGDEMLEKVAYLHKREGQMQYQQYRAGGWPIGSGIAESGNKLVVQARL
KGAGMHWARPQVNAMLALRTTLCSNRWTQDWHVVRASWHTSRLQRFRARSQAALAKASLRLQQTFWRLPLPLILACFPSPPPTSMAPKGRTEGQRRWGRQ
TFSQRAIQDGRYAKK
KLSRRYGTCPQCGSGLFPLDEELNLPSSGLTPHAHQGLVLLGSMVPYACAATHLETLLKVQVSKSTVRRLTEQAGACLQQWQDQYACPGQGSEQPKQAAP
RQAMATDGVLIPVLPNEWAEVKMVTIGEVCQTKSGEPHCEKLSYFARLADAETFGDLASFEIQRRRVDQAREVAAINDGAEWIQGFVQGHRADAVRILDF
AHAAQYISEIATLAQNAGVPLPPTWVSEQWHKLKEDGPTSVLKEVRRLSASTPGDEMLEKVAYLHKREGQMQYQQYRAGGWPIGSGIAESGNKLVVQARL
KGAGMHWARPQVNAMLALRTTLCSNRWTQDWHVVRASWHTSRLQRFRARSQAALAKASLRLQQTFWRLPLPLILACFPSPPPTSMAPKGRTEGQRRWGRQ
TFSQRAIQDGRYAKK
Blast result :
Comments
ISKra4 is 55% aa similar to ISKra2.
The third ORF is the putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
The third ORF is the putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
References
1] ISfinder annotation (2013)
2] Lucas,S., Copeland,A., Lapidus,A., Glavina del Rio,T., Dalin,E., Tice,H., Bruce,D., Goodwin,L., Pitluck,S., Kyrpides,N., Mavromatis,K., Ivanova,N., Chertkov,O., Brettin,T., Detter,J.C., Han,C., Land,M., Hauser,L., Markowitz,V., Cheng,J.-F., Hugenholtz,P., Woyke,T., Wu,D., Jando,J., Schneider,S., Sikorski,J., Klenk,H.-P. and Eisen,J.A. (2010) Direct submission GenBank.
2] Lucas,S., Copeland,A., Lapidus,A., Glavina del Rio,T., Dalin,E., Tice,H., Bruce,D., Goodwin,L., Pitluck,S., Kyrpides,N., Mavromatis,K., Ivanova,N., Chertkov,O., Brettin,T., Detter,J.C., Han,C., Land,M., Hauser,L., Markowitz,V., Cheng,J.-F., Hugenholtz,P., Woyke,T., Wu,D., Jando,J., Schneider,S., Sikorski,J., Klenk,H.-P. and Eisen,J.A. (2010) Direct submission GenBank.