ISfinder

ISKpn21

Family IS1202
Group ISAba32

MGE type ISRelated element(s) :
Isoform Synonym(s) ISKpW

Accession number	Transposition	Origin	Host
AP012055	ND	Klebsiella pneumoniae	Enterobacter cloacae plasmid pEC-IMP Klebsiella pneumoniae subsp. pneumoniae KPX plasmid pKPX-1 Klebsiella pneumoniae ATCC BAA-2146 Klebsiella pneumoniae ST101 plasmid pKPN101-IT Klebsiella pneumoniae subsp. pneumoniae MGH 78578 Klebsiella pneumoniae subsp. pneumoniae MGH 78578 plasmid pKPN3 Klebsiella pneumoniae JHCK1 Klebsiella pneumoniae subsp. pneumoniae WGLW5 Klebsiella pneumoniae plasmid pNDM-MAR Klebsiella pneumoniae subsp. pneumoniae 12-3578 Klebsiella pneumoniae 1162281 Klebsiella oxytoca E718 Klebsiella oxytoca E718 plasmid pKOX_R1 Klebsiella pneumoniae TCKpnC Klebsiella sp. MS 92-3 Klebsiella sp. KTE92 Klebsiella sp. 4_1_44FAA Klebsiella sp. OBRC7 Citrobacter freundii GTC Citrobacter freundii plasmid pNDM-CIT Escherichia coli plasmid pNDM-1_Dok01 Escherichia coli KTE111 Escherichia coli MS 175-1 Salmonella enterica subsp. enterica serovar Worthington plasmid pB1016 Enterobacter cloacae plasmid pEC-IMPQ

DNA section

IS Length : 2278 bp

Ends

IR Length : 25

IRL : TGTAATGGCTCAATAGTAATGTCCGCCTGTGGCTCAATTCAGATGTCCGC
IRR : TGTCAAGGCTCAATTGAAATGTCCGTTATCCAGCTCAATTAAAATGACCA

Insertion site

Left flank	Direct repeat	Right flank	DR Length
TATGCGTACT	CATGT	TGTAGTAGCT	5
TATGCGCACC	TGTAT	TGTAATGGCT	5
TAAACGCACT	AATAC	TGTCAGCACA	5
TATACGAAAT	GCTTG	ATATTTCTCT	5
AGCCTTGACA	TATAAA	TGTAGTAGCA	6
TATACGCACT	TATAA	ATGTAATGGC	5
TATGCGCACC	TGTAT	TGTAACTCCA	5
TATGCGTACT	CATGT	TTGTAGTAGC	5
TATGCGCACT	AAGTC	TTTGATTTTA	5
TATACGAAAT	GCTTGA	TATTTCTCTT	6
TATGCGCACC	AAGAT	TGTAGGGGCT	5

DNA sequence

TGTAATGGCTCAATAGTAATGTCCGCCTGTGGCTCAATTCAGATGTCCGCGCTATGGTAAGCTTCACTGGTCCGTTTAAACTACCGGGAGGCATATCATG
AGCGCAGAAAGCTCAGGAGTGTTTACTTTGAAAGAGATCAACCGGATCAAGATTATACAGGACGTCATTGAACGTCGCATCACAACGCGCCGTGCGGCCG
AGCACCTCGGTATCAGCGACAGGCAATGCCGCAGACTTCTTGCCCGTTACCGTGAAGGCGGACCGCTTGGTATGGCCAGCAGACGATGTGGCATGCGTGG
TAACCGCCAGTTGCCACCCGGGCTCGCAGATCAGGCTCTGGAACTGATCAAGACGCGTTATGCTGATTTCGGTCCGACTCTGGCGCGTGAAAAGCTCGAA
GAACTCCACGGACTGTTTCTTGGCAAAGAAACTGTCCGGCGCATCATGGTGCGGGCTGGCTTATGGGTTCCCCGTAAACAACGTGCCGCAAGGATCCCTC
AACCACGGTACCGGCGTCCGTGTACTGGTGAGCTGATACAAATAGATGGCTGTGATCACGACTGGTTTGAAGGCCGTGGCCCGGCCTGCACCGCGCTGGT
CTATGTTGATGATGCAACCAGCAAACTGATGGAACTGTTGTTTGTTAAATCGGAGTCCACGTTTTCTTACTTCGAAGCCACGCGGCGCTATATCGATAAG
CATGGTAAACCGCTGGCACTGTACAGCGATAAAGCCGGTGTTTTTCGTGTTAACAATAAACACGCCACAGGCGGAGACGGGCATACTCAGTTTGGGCGAG
CCATGCATGAACTGAACATCCAGACTATCTGTGCAGAAACCAGTCCCGCCAAAGGGCGTGTAGAACGAGCTCACCTCACTTTACAGGATCGTCTGGTCAA
AGAGCTGCGGTTACAGGGCATTTGTTCAATGGAGGCTGCAAATGACTTCGCTGAGGCCTATATGGCTGACTATAACCGCCGTTTTGGCAAAGTACCGCGA
CATGATTTTGACGTACACCGTGCTGTAGAACATGATGAGGACCTGGGGCTTATTTTCACTGTTCGTGAAAAACGTAAAGTCTCAAAATCGTTGACGATAC
AATATGATAAAATGTTGTACCTGATTGAAGACAGCGAACTGAGTCGCCGTGCAATAGGTAAATATATCGATGTGTATCACTATCCTGATGGCAGAAAAGA
GCTGCGCCTGAACGGTACGCTACTTCCCTACTCTACCTACGACCGACTGTCAGAAATCGACCAGGGCGCGATTGTCGATAACAAGCGTCTTGGCCGAACC
CTGGAGTTTATCAGTCTGGTGCAGAGCAAGCGGGATAACACGCGCTCTCAGTCAATTCCCGCTGGAGATGGCCCTTCCCGACGACGGCCAAAGCAGGAAG
GGAAGAAATCCCAGCGCTCACTGGATAATGATGACATGCTCGAAGCACTCAAACAGCTTCAGTCACGTTCAGAGGACATTTTTGGTAAAAGAGCCCGCTG
ATGGTACTCACTGGCCGGACAGTGGTTGCTCACCAGTTGATATTGTCCGGCCAGACCCATGATGTATTATTCATTTCGTTCAGCATCTTTATGTCCTGCT
TTTCACGGGCATACTTACCCCATAATGAATGAAAAGAACGAACAATACTGATTAACTGCTTTTTTCTTTGCTCCGGAGATAGGCTGATAAAATAGCAGCA
CCTGCTGTCAATATTATCCTCAATGAAATTAAAATCAGAGTCTGATGCATATTTTACTGTTAGCATAATATCATTCGACGTTGTTCTCTCAGACTCTGTT
AATGCTGATTCTATCAGGTATTCAAAGGCATACATGACAGACAGCTGGTTTTTGTCTGTCTGAATATAACCCATTGAGTTTCTGAGTATTGAGGACTGTA
CCAGGACCAGAGGCCATAAAAGCTCAACACCATAAAGCTTATATGTATCATGTTGATCATACTGATGTTGCTCAACACGACGATTACCTTCCCGTGCTTT
ACGAATATCATAGGCAAGGGATAACATAAATCCGTCTTTAACTTTAATCAGTGGGCTTTCATCCACGATGTAATGAATGAGTTCATGTAATTCATTCAGG
GCTTCTGAATCTCCCCACAGTATAAAACCTGCATTGTTCGGCGTTAACTCGTATCGAAGCATGGGCTCAATCATCCTGTTGTTTCAGTCGGAGGAAAGTC
TATCAGTAAAGAGAATGAGAGATCAAAGTGGTCATTTTAATTGAGCTGGATAACGGACATTTCAATTGAGCCTTGACA

Protein section

ORF number : 2

ORF 1

Length		Begin	End	Strand	Fusion ORF
1404 bp	467 aa	98	1501	+	No

ORF function : Transposase
Chemistry : DDE

ORF sequence :

MSAESSGVFTLKEINRIKIIQDVIERRITTRRAAEHLGISDRQCRRLLARYREGGPLGMASRRCGMRGNRQLPPGLADQALELIKTRYADFGPTLAREKL
EELHGLFLGKETVRRIMVRAGLWVPRKQRAARIPQPRYRRPCTGELIQIDGCDHDWFEGRGPACTALVYVDDATSKLMELLFVKSESTFSYFEATRRYID
KHGKPLALYSDKAGVFRVNNKHATGGDGHTQFGRAMHELNIQTICAETSPAKGRVERAHLTLQDRLVKELRLQGICSMEAANDFAEAYMADYNRRFGKVP
RHDFDVHRAVEHDEDLGLIFTVREKRKVSKSLTIQYDKMLYLIEDSELSRRAIGKYIDVYHYPDGRKELRLNGTLLPYSTYDRLSEIDQGAIVDNKRLGR
TLEFISLVQSKRDNTRSQSIPAGDGPSRRRPKQEGKKSQRSLDNDDMLEALKQLQSRSEDIFGKRAR

Blast result :

ORF 2

Length		Begin	End	Strand	Fusion ORF
645 bp	214 aa	2174	1530	-	No

ORF function : Passenger Gene
Annotation : Hypothetical proteinDescription :

ORF sequence :

MIEPMLRYELTPNNAGFILWGDSEALNELHELIHYIVDESPLIKVKDGFMLSLAYDIRKAREGNRRVEQHQYDQHDTYKLYGVELLWPLVLVQSSILRNS
MGYIQTDKNQLSVMYAFEYLIESALTESERTTSNDIMLTVKYASDSDFNFIEDNIDSRCCYFISLSPEQRKKQLISIVRSFHSLWGKYAREKQDIKMLNE
MNNTSWVWPDNINW

Blast result :

Comments

ISKpn21 is 64% aa similar to ISMex6.
The second ORF is a passenger gene annotated as hypothetical protein.

References

1] Kelly Williams (2013) Direct submission
2] Huang,T.W., Chen,T.L., Chen,Y.T., Lauderdale,T.L., Liao,T.L., Lee,Y.T., Chen,C.P., Liu,Y.M., Lin,A.C., Chang,Y.H., Wu,K.M., Kirby,R., Lai,J.F., Tan,M.C., Siu,L.K., Chang,C.M., Fung,C.P. and Tsai,S.F. (2013) PLoS ONE 8 (4), E62774.