ISKpn24

  • Family IS66
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
NC_014312 ND Klebsiella pneumoniae
Klebsiella pneumoniae pK29
Klebsiella pneumoniae pKP048
Klebsiella pneumoniae HS11286
DNA section
IS Length : 2454 bp

Ends


IR Length : 15/22

IRL : GTAAGCGTCGTCTCAGCACCGTCTGGCAGATCCTGAAATTCCTGAGAGGA
IRR : GTAAGCGTGCAGCGAGAACCGTATTGACGGGGATGTGTTATTCAGTTGGC

Insertion site


Left flankDirect repeatRight flankDR Length
CGCTCTTTTTTGACAAAGTC0
TCCAGCGGCGCTTAAAGTAGAGATTGGT8
ATCATTAATGTCTGATGTAA0

DNA sequence

GTAAGCGTCGTCTCAGCACCGTCTGGCAGATCCTGAAATTCCTGAGAGGATAATGGACACCAAATATGGTGGACGCTATCCATGAAATCATTAACCGCAG
TGCGTAAAAAAAGCCCTAATTATCCCGTTGAGTTCAAAATCAAAATGGTTGAACTCTCGCATCGACCAGAGATCTCCGTAGCGCAACTCGCTCGTGAGCA
TGGGATCAACGATAATTTGCTGTTCAAGTGGCGCCAGTACTGGCGCGAAGGAAAACTACGTCCTCCTTCAACAACAGAAAACAACGTGCCTGAGCTGCTC
CCGATAACACTTGATGCCGAAGATGTTGTCCCTGCAACCTCCCCCCGGTCACAACCTGTAGCTGCTGCGGCACCTGAATCACTCAATATCAGCTGTGAAG
TGACGTTCCGGCACGGATCACTCCGTCTGAATGGTGCCATCAGCGAAAATATCCTGAACCTGCTGATACGGGAGCTCAAACGTTGATCCCATTACCATCA
GGGACAAAGATCTGGCTGGTCGCTGGCATCACCGATATGAGAAACGGCTTCAACGGCCTGGCGGCAAAGGTGCAGACGACGCTGAAAGACGATCCGATGT
CAGGTCACGTTTTTATCTTCCGTGGGCGTAATGGCAGTCAGGTAAAGCTCCTCTGGTCTACCGGCGATGGACTGTGTCTGCTGACCAAACGGCTGGAGCG
CGGCCGCTTCGCCTGGCCGTCAGCCCGGGATGGCAAAGTGTTCCTCACACCGGCACAGCTGGCGATGCTCCTTGAAGGTATCGACTGGCGGCAGCCTAAA
AGACTGCTTACGTCACTGACTATGTTGTAGGCCTCTTTATCCTGGTCGACGCTGAATGAGCCTGGTAATATACCCGGTATGAACAGCTTACTTCCTGACG
ATATCGATGAACTGAAACGTCTCCTTGCCGAACAGGAGGCGCTGAACCGTGCCCTTCTGGAAAAGCTGAACGAGCGTGAACGCGAAATAGATCACCTGCA
GGCGCAACTGGATAAGCTGCGCCGGATGAACTTCGGCAGCCGCTCCGAAAAAGTCTCCCGCCGTATCGCGAAGATGGAAGCCGACCTTAAGCAGTTGCAG
AAAGAAAGCGATACCCTTACCGGCCGGGTGGATGACCCGGCCGTGCAGCGCCCGCTGCGGCAGACCCGTACCCGCAAACCGTTCCCTGAATCACTTCCCC
GTGACGAAAAACGGCTGCTGCCGGCAGCGTCATGCTGCCCGGAATGTGGTGGTGCGCTGAGTTACCTGGGTGAAGATGCCGCCGAACAGCTGGAGCTGAT
GCGCAGCGCCTTCCGGGTTATCCGGACAGTACGTGAAAAGCATGCCTGTACTCAGTGCGATGCCATCGTGCAGGCCCCCGCGCCTTCACGGCCCATCGAG
CGGGGTATCGCAGGACCGGGGCTGCTGGCCCGCGTGCTGAGTTCAAAGTATGCAGAGCACACCCCGCTGTACCGCCAGTCTGAAATATACGGCCGCCAGG
GTGTGGAGCTGAGCCGCTCACTGCTGTCGGGCTGGGTGGATGCGTGTTGCCGGCTACTGTCACCGCTGGAAGGGGCGCTTCAGGACTATGTGCTGACTGA
CGGTAAGCTCCATGCTGATGACACGCCTGTCCCGGTGCTGTTGCCAGGTAATAAGAAAACGAAGACCGGGCGGTTGTGGACGTACGTTCGTGACGACCGT
AACGCCGGGTCAGAGCTGGCGCCGGCAGTGTGGTTCGCTTACAGCCCGGACAGAAAAGGTATCCACCCGCAGAGCCATCTCGCTGGCTTCAGCGGTGTTC
TGCAGGCGGATGCGTACGCCGGGTTCAACGAACTGTACCGCAATGGACAGATAACGGAAGCTGCCTGCTGGGCTCATGCCCGCCGCAAGATCCACGATGT
GCACGTTCGCACCCCGTCAGCACTGACGGAGGAAGCCCTGAAACGGATCGGTGAGTTATATGCCATCGAGGCGGAAATAAGGGGGATGCCGGCGAAGCGA
CGCCTTGCAGAACGTCAGCAAAAAGCTAAACCGCGGCTGAAATCCCTGGAAAGCTGGCTGCGTGAAAAGGTGAAAACGCTGTCGCGACACTCAGAACTGG
CGAAAGCGTTCACGTACGTACTGAACCAGTGGCCGGCGCTGGCTTACTATACTGACGACGGCTGGGCCGAGGCAGATAACAACATAGCTGAGAATGCGCT
ACGGATGGTCAGCCTGGGCCGCAAAAACTACCTGTTCTTCGGTTCGGATCATGGAGGAGAGCGGGGAGCGCTGCTGTACAGCCTGATCGGGACATGCAAA
CTGAACGGAGTGGAGCCAGAAAGCTACCTTCGCCATGTGCTTGATGTTATAGCTGACTGGCCGATAAACCGGGTCAGCGAACTGCTCCCCTGGCGCGTAG
CACTGCCAACTGAATAACACATCCCCGTCAATACGGTTCTCGCTGCACGCTTAC
Protein section
ORF number : 3

 

ORF 1
LengthBeginEndStrandFusion ORF
405 bp134 aa82486+No
ORF function : Accessory Gene
AG : IS66 TnpA

ORF sequence :

MKSLTAVRKKSPNYPVEFKIKMVELSHRPEISVAQLAREHGINDNLLFKWRQYWREGKLRPPSTTENNVPELLPITLDAEDVVPATSPRSQPVAAAAPES
LNISCEVTFRHGSLRLNGAISENILNLLIRELKR

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
348 bp115 aa483830+No
ORF function : Accessory Gene
AG : IS66 TnpB

ORF sequence :

MIPLPSGTKIWLVAGITDMRNGFNGLAAKVQTTLKDDPMSGHVFIFRGRNGSQVKLLWSTGDGLCLLTKRLERGRFAWPSARDGKVFLTPAQLAMLLEGI
DWRQPKRLLTSLTML

 

Blast result :
ORF 3
LengthBeginEndStrandFusion ORF
1539 bp512 aa8792417+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MNSLLPDDIDELKRLLAEQEALNRALLEKLNEREREIDHLQAQLDKLRRMNFGSRSEKVSRRIAKMEADLKQLQKESDTLTGRVDDPAVQRPLRQTRTRK
PFPESLPRDEKRLLPAASCCPECGGALSYLGEDAAEQLELMRSAFRVIRTVREKHACTQCDAIVQAPAPSRPIERGIAGPGLLARVLSSKYAEHTPLYRQ
SEIYGRQGVELSRSLLSGWVDACCRLLSPLEGALQDYVLTDGKLHADDTPVPVLLPGNKKTKTGRLWTYVRDDRNAGSELAPAVWFAYSPDRKGIHPQSH
LAGFSGVLQADAYAGFNELYRNGQITEAACWAHARRKIHDVHVRTPSALTEEALKRIGELYAIEAEIRGMPAKRRLAERQQKAKPRLKSLESWLREKVKT
LSRHSELAKAFTYVLNQWPALAYYTDDGWAEADNNIAENALRMVSLGRKNYLFFGSDHGGERGALLYSLIGTCKLNGVEPESYLRHVLDVIADWPINRVS
ELLPWRVALPTE

 

Blast result :
Comments
ISKpn24 is 96% aa similar to ISEc22.
References
1] ISfinder annotation (2014)
2] Jiang,Y., Yu,D., Wei,Z., Shen,P., Zhou,Z. and Yu,Y.(2010) Antimicrob. Agents Chemother.