ISfinder

ISKpn25

Family ISL3
Group

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
NC_009650	ND	Klebsiella pneumoniae	Klebsiella pneumoniae subsp. pneumoniae MGH 78578 plasmid pKPN4 Klebsiella pneumonia subsp. pneumoniae MGH 78578 Klebsiella pneumonia plasmid pKpQIL

DNA section

IS Length : 8154 bp

Ends

IR Length : 21/24

IRL : GGCTATTTGCATTCTGAGGTAGGATCGCAAATTTAGCCAAGAATTGAAAT
IRR : GGCTATTTGCATTCTAGGGTTGGAATTCATCCATTCTAACCCTTAATTCT

Insertion site

Left flank	Direct repeat	Right flank	DR Length
TCCATTTTTG	GAAATTAG	TTTGATTTAT	8

DNA sequence

GGCTATTTGCATTCTGAGGTAGGATCGCAAATTTAGCCAAGAATTGAAATCCATCCCCTTTTGTGCAAAATCTGGTTGACATTCGCAGATTGACTACAGT
ACCATTCTGTGCAAAAGGTGGTTGGAGGCCAGAATGAACGAATTTACGGGATCGGCAGCTTCACAGGCTGACTTTATTTGGAAGAATGCGGAAGACCTGT
GGGGTGACTTCAAGCATACGGACTTTGGCAAGATCATCTTGCCGTTTACCCTGCTACGCCGTCTGGAGTGTGCGCTGGAGCCGACCCGCGAGGCGGTGAG
AGAAGCGCATGACGCTTTCAAGGATGCCGATGTTGAGCTGGATACCATTCTGCGCTCAACCGCTGAATACCCCTTCTACAACACCTCTGAATACTCCCTC
GGCACCCTGGGTAGCACCAAGACGCGCCGCAATCTGGAAGACTACATCGCCCTGTTTTCGGATAACGCCCGCGCTATCTTCGAGGAGTTTGAGTTTGGCA
ATACGGTGATCCGGCTGGAGAAAGCGGGCTTGCTGTATAAGATTTGCCAGAACTTCGCCAAGATCGACCTGCACCCGGACGTGGTGCCGGATCGGGTGAT
GAGTAACATCTACGAACACCTGATCCGCCGCTTTGGTGCTGAGGTCAATGAAGGAGCCGAGGACTTCATGACGCCGCGTGACATCGTTCACCTGGCGACC
GCATTGCTGCTTGACCCGGATGATGCCCTGTTTGAGGCCAGCCCAGGTTTGATTCGTACCCTGTATGACCCGACCTGTGGCACGGGTGGCTTCCTCACCG
ATGCTATGAACCATGTGGGGGACTACGGTAACCGCGACAAGATCCCGCCAGTGCTGGTGCCGCACGGGCAGGAGTTGGAGCCGGAAACCCATGCGGTTTG
TGTGGCCGGTATGCTGATCCGCCGTTTGGAGTCGGACCCTGGCCGAGATCTGTCGAAGAACATTCGTCAGGGCAGCACGCTGTCCAACGACCAGTTTGCC
GGTGAGCGTTTCCACTACTGCCTGTCCAATCCGCCTTTCGGCAAGAAATGGGAGAAGGACAAAACCGCTGTCGAAGCGGAACACAAAAAAGGCGAGCTGG
GCCGGTTTGGTCCGGGCCTGCCGAAAATTAGCGATGGCTCCATGCTGTTTTTGATGCACCTGGCGAGCAAGCTAGAACTGCCGATCAACGGCGGTGGCCG
CGCCGCTATTGTGCTGTCCGGTTCGCCACTGTTTAACGGCGGTGCCGCGTCTGGCGAATCGGAAATCCGCCGTTGGTTGCTGGAAGACGACCTGATTGAA
GCCATTGTAGCTCTGCCAACGGATCTGTTCTTCCGCACCAATATCGCCACCTACCTGTGGATTCTGTCCAACAAGAAGCCTCAGGAGCGCAAAGGCAAGG
TGCAGTTGATCAACGCCACCGACCTGTGGACTTCAATCCGCAACGAGGGCAACAAGCGCCGTATTGTCAGCGATGAGCAGCGCCGCCAGATTCTGGACAT
CTACGCAGCAGGCGAAACCGGTGCGCTTTCCCGGATGCTGGACTACCGTACCTTTGGCTACCGCCGTATCAGGGTCCTGCGCCCGCTGCGCATGACCCTA
GAGCTGGATAAGGTGGGTATGGAGCGGTTGGAAGCCGAAGCTGCCTGGGAAAAGCTCTCTGACGCGCATCAGACATTCTGGCGCGAAGCCCTCAAGCCGC
TGATCGGGCAAACGCAGCCCTATAGCTGGGCAGAAACCTTTGTTAGCAACTCGATCAAGTCCGATGAAGCCAAGCAGCTCAAGGTCAAATCCAACAAGAC
ATTGATCACCGCGCTAATCAACGCCTTTGGTCACAAAGACCCGAAAGCCGAGCCAGTCACGGATTCAAACGGCGAGCTGGTGCCAGACACAGATTTAACT
GATTACGAGAACGTCCCCTATCTGGAGGACATCGACGACTACTTTGCCCGCGAAGTGCTTCCTCATGTGCCGGATGCCTGGCTGGATGAGAGCTTTACCG
ATGCCAGGGATGGTCAATTGGGCCGCGTTGGCTACGAGATCAACTTCAACCGCTTCTTCTACCAGTACCAGCCGCCGCGCAAGCTGCATGATATTGATGA
AGACCTGAAGCAGGTAGAAGCCGAGATTGCCGCGCTACTGGCGGAGGTGGCCAGCGAATGAGTCAGTACAAAGCGTATACCTCATACAAAGATTCAGGCG
TTGAGTGGATTGGGCAGGTGCCGGAGCATTGGGAGGTGAAAAGGTTACGTCATGTCGGGCGTTACTCTAATAGTGGCGTTGACAAGAAGAGCTACGAAGA
CCAGCAGACGGTTGAGCTTTGTAACTACACGGATGTCTATTACAACGAGTTCATCAGTGATGATATGCCTTTCATGCAGGCTACTGCCAGCGCCCACGAG
ATTGAGCAGTTCACTCTGAAAAAAGGCGATGTCATTATCACGAAGGATTCAGAAGACCCATCCGACATAGGGATTCCTGCCTTTGTTCCACATGATATGC
CCGGAGTGGTTTGTGGCTATCATCTCACTATGATTCGTGCCTTAAATGATAACTATGGAAGCTATATCCATCGCTCGATCCAGTCGGATCATACAAGAGC
TCACTTCTTTGTTGAGTCGCCAGGAATAACCCGATATGGCCTGAATCAAAATACTATTGGTAATGCCCCAGTAGCGCTCCCTCCACCAGAAGAACAAGCC
ACCATCGCGGCCACCCTCGACCGCGAAACCGCCCGTATTGATGCGCTTGTCGAGAAGAAAATCCGCTTTATCGAGCTACTGAAGGAAAAGCGCCAGGCGC
TGATTACCCATGCGGTCACCAAGGGCCTAGACCCCAACGTGAAGATGAAGGATTCCGGCGTTGAGTGGATTGGGCAGGTGCCGGAGCACTGGGAGGTTAA
ACCCTTCTTCGCGTTGGTGTCAGAACTGAACCGAAAGAATGTGGGCCTAGCTGAAACCAATATACTGTCCCTCAGCTACGGAAACATCATCCAGAAGCCT
GAAACCAGAAATATGGGATTAACACCAGAGTCTTATGAAACCTATCAAATTGTAGAGTCTGGCGAAGTTGTATTTAGGTTCACCGATCTGCAAAACGACA
AACGTAGCTTGCGTTCAGCCCAGGTTACGCAAAGAGGAATCATTACCTCTGCGTATATGGCCGTGAAACCGCACAGTATTGGTTCTACATATTTTGCATG
GTTGATGCGTTCATATGACCTTTGTAAAGTCTTTTATGCAATGGGTGGAGGATTGAGGCAGTCATTGAAGTTTGAAGACGTCAGAAGGCTTCCTGTACTT
ATCCCTCCAGTCGGTGAACAGAGTGAAATTACCAATACAATTAATGCAGGAACTGCTCGCATTGATGCTCTAGTCGAGAAAACAGAACAAAGTATCACCC
TGCTCAAAGAACGTCGCGCTGCCTTTATCACCGCCGCTGTCACCGGCCAGATTGATTTGCGAGGAAAACAATAAATGCAATCAGCAGCACATCAGGAAAA
ACACTTCCAGCAATACATCATCGACCGGTTGGTAGAGCAGGGCTGGAAGCTGGGTGATTCCAAGTTCTACGACACCGAGCGGGCGGTGTACCCCGAAGAC
CTGGAAAGCTGGATCAAAACCAGCGGCCAGCAGGAAAAATGGGACAAGCTGGAACGGCTCAACGGGGCCAAAACCCTTGAAGTGCTGATGGATCGCTTGG
ATAAAGCACTGGAGAAACAAGGCACCATGCAGGTGTTGCGCCAGGGTTTTTCCATTGCCGGTTGCGGCCTGATCGAGATGACCGAGGCTGCACCGGAAGA
CAAGCGCAATGCAGCAGTCATTGAGCGCTACCAGGCCAATATCCTGCGCGTGGTGCCGGAGCTGAAGTATCACCCGGCCCGTGAATTTGCCATTGATCTG
GTGCTGTTTATCAACGGTCTGCCGGTGGCAACCGTCGAACTGAAGACCGACTTCACCCAGTCCTGCGAAGCGGCCATGGATCAGTACCGCAATGATCGCC
TGCCGTATGACGCCAAGACCAAACGCCGGGAGCCATTGCTGACCTTCAAGCGTGGGGCGGTGGTGCATTTCGCCATGTCCGACTCTGAAATCATGATGGC
GACCAAGCTGGATGGAGAAAACACCTTTTTCCTGCCGTTCAATAAGGGGCGCAAGGACGAAAGTGGCACGGTACATGCGGGTAACCCGCCAGGTGAGATT
AAGGCCGATGGTACCCAGGAATACCCGGTAGCCTACTTCTGGGAGGCGGTCTGCCAGCCGGATGCCTGGCTGCGGATTTTCCATAGCTTTGTCTACGTCG
AGAAAAAGGACGTTGTGGATATTCAGGGCAACTGGTCGAAGAAAGAAACCCTGATTTTCCCGCGCTTCCACCAATGGACGGCAGTGAACCAGATGCTGAC
CGATGCCCGCGAAAACGGCGCAGGCATGACCTACCTGTGTGACCACAGCGCCGGTTCCGGCAAGACCAGCACCATTTCCTGGACGGCCCATGACTTGGTG
AAACTGCGCGAAGATAACGGCGATTCGGTATTTAACAGCGTGATCATTGTCACCGACCGCAATGTGCTGGACGGCCAGCTTCAGGACGCGGTGAAGCAGA
TTGACCATCAGTTTGGGGTGATCGCCGCCATTGACCGGCAGAAGTCATCCAAGTCCAAGAGCAAGCAACTCTCGGAGGCCTTGTTGTCTGGCACGCCGAT
TGTGGTGGTCACGATCCAGACCTTCCCCTACGCGATGGAAGCCATCATCACTGACAAGGCCCTGAAAGGGAAAAACTTTGCTGTGATCATTGATGAGGCG
CATAACTCGCAGACAGGCTCTACCGCCGCTAAGCTGCAAGCCGCGCTGGGGATGAGTGGGCAAGGCAAGATGTCCACCATGACGGTGGATGAATTGCTGG
AACAACTGCAAAAATCTCGGGCTCGCCCGGACAATATCAGCTATTTCGCCTTTACCGGTACGCCGAAGCACTCCACTTTGATGCTGTTCGGTCGCCCAAC
AGACCCGAGCCAGCCAGCCTCTAACGATAACCCTCCACAAGCCTTCCACCTGTACACCATGCGCCAGGCCATTGAGGAGAAATTCATTCTCGATGTGCTG
AAAGGCTATGTGCCCTACAAAACGGCCTTCAACCTTTCCAAGCAGCTTGAAGACAGCAAGCGGGTAAGCGGCAAGGCCGCCAAACGCGCCCTGGCACAGT
GGATGTCATTGCATCCCACCAATGTGACCCAGAAGGTACAGTTTATCGTTGAGCACTTCACCAAAAACGTCGCCCACCGGCTGGATGGCAAGGCCAAGGC
GATGGTCGTGACCAGCTCCCGCGCCGCTGCCATTCGCTACAAGAAGGCGTTTGACCGCTATATCGAGCAGCACAGCGAGTACGGCTTTATTCATTCACTG
GTGGCCTTCTCCGGCAAGATGACCGGTAAGCAGGTGATGCACCAGGATGACAGCGAGTTCAAGGATGATGTGTTTATCGTCGATGAGAACGAAGAGTTCA
CCGAGCAGAGCATGAACCCGGATGTCCAAGGGCAGGATTTGCGCTTTGCCTTTGACCGACCTGAATACCGGGTGATGTTGGTGGCTGACAAGTTCCAGAC
TGGCTTTGATCAACCCAAACTGGTCGCCATGTATGTAGACAAGAAAATCGCTAACCATGTGGAGATTGTGCAGACCTTCTCCCGTCTCAACCGGACGGCG
CCCGGTAAGGATGAGGTCTTTATCATCGACTTTGTGAACGACCCGGAGAACGTGCGTCAGGCCTTTACCACCTATGACAAGGGTGCTCACATCGACGAAG
TGCAAGACCTCAATGTGGTCTACGAGATCAAGGAGCGCTTGGACGAGCACGGCCTGTACGACGAGAAGGACTTGGCTGCGTTCAAGGAAGCACGCTTCAA
GACCATCCGCGACATCACCCATACCAAATCGCCACAGCACAAGGCGCTGTATGCCGCCACTGCTGGAGCGACGGCCTTGTATAACGACAAGATGAAGATG
CTGCGCGATGGCATGGCGACCTGGGAGGCGGCTTTTGAGAAGGCCCGCGCCAAGGGTGATGAGGCGGGCATGAAGTCTGCTGACCACCATCAGGATGAGT
ACGCCGAGCAAATCAAGGCACTGATTGGCTTCAAGTCGGATTTGGGGCGTTTTTGTCGTACCTACTCCTATATCGCCCAACTGATTGATTTTGGCGACCC
GGAGCTGGAGAACTTCGCCGCCTTCGCCAAGCTATTGCAAAAGCGCCTTCAGCACGAAGCACCTGAAACCGTGGACTTGACCGGCCTGGTGCTCACCGGT
TTTGACATCAATGCTCGTTCAGATAACGCGGAGGACGAAGGCGAAACCCCGGTACTGCAACCGGTAGGTGCAGGCGGTGGTGGTGTGGCGGGCGACAAGC
CGAAATTCGTCAAAGAGATTATTGAGCGACTCAACAGCCTGTTTGGTGAGGCAACGCCGATCCAGGATCAGGTGGCCTTCGTGAATCAGATCTTTTCAAT
CGCCGGCGAAAGCGATGTGGTGATGGCTCAGGTGGAAAGTAACACCCGCGAACAGGCCATGAAAGGCAATTTGCCCGGCGCTGTTCAGCAAGCGGTGGTT
CGTGCTTTGTCCAGTCATCAGAAGCTGGCAACCCAAGTGCTCAAGTCTGACCGTCAGGGCATGACCGCATTGGTTGATATGGTGTATGACCTGCTGCGCG
AAGGCAAAGACATCGATCTGGGTATGGATTAAGCCGATGCTTGACCTGCTGAATCTGCCAGGCATCAAGCCGGTCGATATGCGCCACGAAGGCAAGTGCC
TTGTGATAGTTGCTGAGCCGGTAACGGTCGAGGTGCCGTTATGTGGGGAGTGCAACATCCCTATGCACAGACACGGTACGCGCAAAAACAAGTTCATGGA
CACGCCGTTGTACATGGAGCCCGTCCGCCTTGAAGTTCAGCGCCCGCGCTTTCGCTGTGAGTCGTGCGGAAAAATGTCCATGCCGGAGTTGAGTTTTCTG
GATGACAAACGCCGAGCCACCAAACGGCTGGTCGATGTTATTCGGCAACAGTGCCTGGGAACCACCTTCCATGCTTTAGCCGAGCAAACCGGTGTGGCGG
TGAACACCGTCAAGAACATCGCTCGCGATCTCATTGAGGAACTCTCCCAAACCGTTCGTTACGAAACCCCGGTCATCATGGGTATTGACGAAGTGAACCT
AGCCGGAGGGTATCGCTGTGTAATCACCAATCTGGCAACCAACAACGTCTTTGATATGTTGGAGCATCGTACTCAAGAGCATTTGAAGCCCTTCTTCAAG
GATTTGCCGGATGCTGACAAGGTGGAATGGGTCTGTACGGATATGTGGCGACCCTTCAAACGTTCCTTTGCTCAGTATCTGCCCAACGCCAAACTGGTGA
TCGACAAGTTCCATGTCGTCAAGATGGCCTCTGAAGCCTTGGAAGCGGAGCGCAAGAACTATCAGGCACAGTTAAGCAAAGAAGATCGCATTTACGTCAA
AAAGTCCATTCGCTGGCTCACCCTGAAGCGACCCGGCAACCTAACGCCCGCAGAGCAAAAGGCGCTGGAGGTTGTAAAGCAGGCAATCCCAGCACTTGCC
ATGGCCTATGACTTCAAGGAAGCCTTCTTCTGCATCTACGACGAACCCGACAAGCAGAGCGCTCAGAACGCCTTTGAGGCTTGGGAGAACAGCCTGCCGC
CTTATGGCATGGAGCCTTTCAAAAAACTGGTAAAAACGGTACACAACCATTACGACGACATCTTTGCCTACTGGGATGCGCCGTTCTCAATCACCAATGG
CTATACCGAAGGGCTCAACGGCTTGATCAAGATGTCCAACCGGTTAGGTCGGGGTTATAGCTATGAGATCATCCGTGCAAAGACGTTGTACTCCAAAGAA
GCCCGCAAGGTTGGCAGTGGTATTCGAGCAGGACGAGGCAAGGTCGAGTATGGACCGCACATTCCGACATTGTTGAAGCAGGCAGAAGGTGGAGAGTTGG
ATTAACGCGAGAGAGGGCGCGTCTGAGAATCCAACCAATGTTAGATTTTCGCAGGGTGGTGAAAAGCAGAAAAATCCAACCAGAAGCAGCTTTTTGTCCG
ACTAAGAATTAAGGGTTAGAATGGATGAATTCCAACCCTAGAATGCAAATAGCC

Protein section

ORF number : 4

ORF 1

Length		Begin	End	Strand	Fusion ORF
2055 bp	684 aa	107	2161	+	No

ORF function : Passenger Gene
Annotation : Putative type I restriction-modification system DNA methylaseDescription :

ORF sequence :

MCKRWLEARMNEFTGSAASQADFIWKNAEDLWGDFKHTDFGKIILPFTLLRRLECALEPTREAVREAHDAFKDADVELDTILRSTAEYPFYNTSEYSLGT
LGSTKTRRNLEDYIALFSDNARAIFEEFEFGNTVIRLEKAGLLYKICQNFAKIDLHPDVVPDRVMSNIYEHLIRRFGAEVNEGAEDFMTPRDIVHLATAL
LLDPDDALFEASPGLIRTLYDPTCGTGGFLTDAMNHVGDYGNRDKIPPVLVPHGQELEPETHAVCVAGMLIRRLESDPGRDLSKNIRQGSTLSNDQFAGE
RFHYCLSNPPFGKKWEKDKTAVEAEHKKGELGRFGPGLPKISDGSMLFLMHLASKLELPINGGGRAAIVLSGSPLFNGGAASGESEIRRWLLEDDLIEAI
VALPTDLFFRTNIATYLWILSNKKPQERKGKVQLINATDLWTSIRNEGNKRRIVSDEQRRQILDIYAAGETGALSRMLDYRTFGYRRIRVLRPLRMTLEL
DKVGMERLEAEAAWEKLSDAHQTFWREALKPLIGQTQPYSWAETFVSNSIKSDEAKQLKVKSNKTLITALINAFGHKDPKAEPVTDSNGELVPDTDLTDY
ENVPYLEDIDDYFAREVLPHVPDAWLDESFTDARDGQLGRVGYEINFNRFFYQYQPPRKLHDIDEDLKQVEAEIAALLAEVASE

Blast result :

ORF 2

Length		Begin	End	Strand	Fusion ORF
1317 bp	438 aa	2158	3474	+	No

ORF function : Passenger Gene
Annotation : Putative restriction endonuclease S subunitDescription :

ORF sequence :

MSQYKAYTSYKDSGVEWIGQVPEHWEVKRLRHVGRYSNSGVDKKSYEDQQTVELCNYTDVYYNEFISDDMPFMQATASAHEIEQFTLKKGDVIITKDSED
PSDIGIPAFVPHDMPGVVCGYHLTMIRALNDNYGSYIHRSIQSDHTRAHFFVESPGITRYGLNQNTIGNAPVALPPPEEQATIAATLDRETARIDALVEK
KIRFIELLKEKRQALITHAVTKGLDPNVKMKDSGVEWIGQVPEHWEVKPFFALVSELNRKNVGLAETNILSLSYGNIIQKPETRNMGLTPESYETYQIVE
SGEVVFRFTDLQNDKRSLRSAQVTQRGIITSAYMAVKPHSIGSTYFAWLMRSYDLCKVFYAMGGGLRQSLKFEDVRRLPVLIPPVGEQSEITNTINAGTA
RIDALVEKTEQSITLLKERRAAFITAAVTGQIDLRGKQ

Blast result :

ORF 3

Length		Begin	End	Strand	Fusion ORF
3258 bp	1085 aa	3475	6732	+	No

ORF function : Passenger Gene
Annotation : Hypothetical proteinDescription :

ORF sequence :

MQSAAHQEKHFQQYIIDRLVEQGWKLGDSKFYDTERAVYPEDLESWIKTSGQQEKWDKLERLNGAKTLEVLMDRLDKALEKQGTMQVLRQGFSIAGCGLI
EMTEAAPEDKRNAAVIERYQANILRVVPELKYHPAREFAIDLVLFINGLPVATVELKTDFTQSCEAAMDQYRNDRLPYDAKTKRREPLLTFKRGAVVHFA
MSDSEIMMATKLDGENTFFLPFNKGRKDESGTVHAGNPPGEIKADGTQEYPVAYFWEAVCQPDAWLRIFHSFVYVEKKDVVDIQGNWSKKETLIFPRFHQ
WTAVNQMLTDARENGAGMTYLCDHSAGSGKTSTISWTAHDLVKLREDNGDSVFNSVIIVTDRNVLDGQLQDAVKQIDHQFGVIAAIDRQKSSKSKSKQLS
EALLSGTPIVVVTIQTFPYAMEAIITDKALKGKNFAVIIDEAHNSQTGSTAAKLQAALGMSGQGKMSTMTVDELLEQLQKSRARPDNISYFAFTGTPKHS
TLMLFGRPTDPSQPASNDNPPQAFHLYTMRQAIEEKFILDVLKGYVPYKTAFNLSKQLEDSKRVSGKAAKRALAQWMSLHPTNVTQKVQFIVEHFTKNVA
HRLDGKAKAMVVTSSRAAAIRYKKAFDRYIEQHSEYGFIHSLVAFSGKMTGKQVMHQDDSEFKDDVFIVDENEEFTEQSMNPDVQGQDLRFAFDRPEYRV
MLVADKFQTGFDQPKLVAMYVDKKIANHVEIVQTFSRLNRTAPGKDEVFIIDFVNDPENVRQAFTTYDKGAHIDEVQDLNVVYEIKERLDEHGLYDEKDL
AAFKEARFKTIRDITHTKSPQHKALYAATAGATALYNDKMKMLRDGMATWEAAFEKARAKGDEAGMKSADHHQDEYAEQIKALIGFKSDLGRFCRTYSYI
AQLIDFGDPELENFAAFAKLLQKRLQHEAPETVDLTGLVLTGFDINARSDNAEDEGETPVLQPVGAGGGGVAGDKPKFVKEIIERLNSLFGEATPIQDQV
AFVNQIFSIAGESDVVMAQVESNTREQAMKGNLPGAVQQAVVRALSSHQKLATQVLKSDRQGMTALVDMVYDLLREGKDIDLGMD

Blast result :

ORF 4

Length		Begin	End	Strand	Fusion ORF
1269 bp	422 aa	6737	8005	+	No

ORF function : Transposase
Chemistry : Unknow

ORF sequence :

MLDLLNLPGIKPVDMRHEGKCLVIVAEPVTVEVPLCGECNIPMHRHGTRKNKFMDTPLYMEPVRLEVQRPRFRCESCGKMSMPELSFLDDKRRATKRLVD
VIRQQCLGTTFHALAEQTGVAVNTVKNIARDLIEELSQTVRYETPVIMGIDEVNLAGGYRCVITNLATNNVFDMLEHRTQEHLKPFFKDLPDADKVEWVC
TDMWRPFKRSFAQYLPNAKLVIDKFHVVKMASEALEAERKNYQAQLSKEDRIYVKKSIRWLTLKRPGNLTPAEQKALEVVKQAIPALAMAYDFKEAFFCI
YDEPDKQSAQNAFEAWENSLPPYGMEPFKKLVKTVHNHYDDIFAYWDAPFSITNGYTEGLNGLIKMSNRLGRGYSYEIIRAKTLYSKEARKVGSGIRAGR
GKVEYGPHIPTLLKQAEGGELD

Blast result :

Comments

ISKpn25 is 82% (ORF4 : the transposase) aa similar to ISSm4. The three others ORF are passenger genes respectively annotated as putative type I restriction-modification system DNA methylase ( ORF1) , putative restriction endonuclease S subunit (ORF2) and hypothetical protein (ORF3).

References

1] ISfinder annotation (2014)
2] McClelland,M., Sanderson,E.K., Spieth,J., Clifton,W.S., Latreille,P., Sabo,A., Pepin,K., Bhonagiri,V., Porwollik,S., Ali,J. and Wilson,R.K. (2006) Direct submission GenBank.