ISHbo4

  • Family IS200/IS605
  • Group IS605
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
CP001690 ND Halogeometricum borinquense
Halogeometricum borinquense DSM 11551
DNA section
IS Length : 1835 bp

Ends


Left end : CTGAATTGAGGCGCTAAGCCCCCTTCCTCAGCGAGCGCCGACCGAACGGGAGGCGCGAGCAGGGAGGGGATACAGCGCCGCACAGTTCTCATACACGATT II struct. : No
Right end : GGATACGCCTGTATCTGCAAAGCGCGTCGTGGAAGCAGGAAGCCCTACCCTCAAGGAGCGAACGGCGTCAGCCGTGAGCGAGTAGGGTAGGGTAGTTCAC II struct. : No

Insertion site


Left flankLE cleavage siteRight flankRE cleavage site
GGTTGTACCCCGTTAGTACGAGCATCGG

DNA sequence

CTGAATTGAGGCGCTAAGCCCCCTTCCTCAGCGAGCGCCGACCGAACGGGAGGCGCGAGCAGGGAGGGGATACAGCGCCGCACAGTTCTCATACACGATT
CGGTGGCAGGCCCACAGGCCCACGCAATCATAACGTTTTTTCAGTAGGGTTGTATAGTATTGGCCGAACCCAATGGAGTACGACCTCGACTCGGGAGCGC
ACTCGACGTATTCCCTGCACTACCACCTGATACTCACCACGAAGTATCGGCGCGGAGTGCTAACCGAGGAGCGAACCCAATTCATTCACGAGGTCATCAG
CGGGTTCACGGACAACTACGGTGTCGAACTGACGAACTTCGACGGCGAGGACGACCACGTACACATCCTGTTCCGAGCGAAGCCAACCACAGACCTCGTG
AAGTTCATCAACACGGCCAAGGGCGCGACCGCCCGCCGTATCCGCAACGAGTACGCGGACGAACTCAAGACCGAACTGTGGGGCGACTCGTTCTGGAACG
ACTCGTATTGCCTCATCTCGACGGGGCAGGTGTCGCTGGATGTGCTGAAACAGTACGTAGAGGACCAACGCGAGTAGAATGTACTACGCCTACAAGTACC
GCCTCAAGCCGTCCGACGCCAACCGTGAGGAGTTGGACCGCCACCGAGACATTTGTAGGCAACTTTACAACCACACGCTCTACCGTCTCAACGAGTACCA
AGACGAACACAGAGAACTGCCGTCCATGACCTCCCTGCGGTCGGAACTCCCCGACCTCAAGGAGTGGTGGGACGACCTCTCAGACGTGTACTCGAAGGTT
CTCCAAACCGTCGTAGAACGGCTGTTCGACAACCTCAAAGGCCTCTCCAAGCTCAAGGAGAACGGCTACAGCGTCGGCCAACTCAAATGGAAGCCGCCAC
GAGAGTTCCGCAGTTTCACGTACAGCCAGTCTGGCTTCAAGCTCGACAAGAAGGGCGGTCAGACTGTGCTGTCACTCTCGAAACTCGCGGACATACCGAT
TCGGCTCCACCGCGCCATCCCCGACGACGCGAAGCTCAAACAGGTCACAGTCAAGAAGGAACAGACGGACGAGTGGTTCGCCACCTTCGGCGTCCAAATG
GACCGTGAACCACCCGAACCGCCTGAGAACCCCGAGAAGTGCGTCGGTATCGACGTTGGGATTCTCAAGTACGCCCACGACACCGACGGCGCGGCGGTCG
GGTCGCTCGACCTCTCAGACGAACGCGACCGCTTGGAGCGCGAGCAACGGAAGCTCTCGCGCAAACAGCACGGGTCGAACAACTACGAGAAGCAACGGCG
TCGGGTCGCGGAGTGTCACGCCGACCTCCGACGGAAGCGCCGCGACTTTCTCCACAAGCTCTCGGCGTACTACGCTCGGGAATACGACCTCGTAGCGGTC
GAAGACCTGAACGTGAAGGGGATGATGGAAAGTCCGTCGAACAGCCGCAACACTGCGTCTGCCGCGTGGCGAACGTTCCTCTCGTTGCTCGAATACAAGT
GCGAGCGTGAAGGAACGCACTTCGTGGCGGTCAACCCGAGAGGGACGACCAAAGAGTGCGCGTCGTGCGGCGTTTCGACCGACAAGCCGTTGTGGGTACG
TGAACACTCCTGTCCCGCCTGCGGGTTTGAGGCGGACAGGGACGCGAACGCGGCATGGAACATCCTTTCTCGCGGTCTACAAGATGTAGGAGTGGGACAC
TCCGAATCAACGCCTGTGGAGACTGCGCTCCCTGTGGATACGCCTGTATCTGCAAAGCGCGTCGTGGAAGCAGGAAGCCCTACCCTCAAGGAGCGAACGG
CGTCAGCCGTGAGCGAGTAGGGTAGGGTAGTTCAC
Protein section
ORF number : 2

 

ORF 1
LengthBeginEndStrandFusion ORF
405 bp134 aa173577+No
ORF function : Transposase
Chemistry : Y1

ORF sequence :

MEYDLDSGAHSTYSLHYHLILTTKYRRGVLTEERTQFIHEVISGFTDNYGVELTNFDGEDDHVHILFRAKPTTDLVKFINTAKGATARRIRNEYADELKT
ELWGDSFWNDSYCLISTGQVSLDVLKQYVEDQRE

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
1242 bp413 aa5791820+No
ORF function : Accessory Gene
AG : TnpB

ORF sequence :

MYYAYKYRLKPSDANREELDRHRDICRQLYNHTLYRLNEYQDEHRELPSMTSLRSELPDLKEWWDDLSDVYSKVLQTVVERLFDNLKGLSKLKENGYSVG
QLKWKPPREFRSFTYSQSGFKLDKKGGQTVLSLSKLADIPIRLHRAIPDDAKLKQVTVKKEQTDEWFATFGVQMDREPPEPPENPEKCVGIDVGILKYAH
DTDGAAVGSLDLSDERDRLEREQRKLSRKQHGSNNYEKQRRRVAECHADLRRKRRDFLHKLSAYYAREYDLVAVEDLNVKGMMESPSNSRNTASAAWRTF
LSLLEYKCEREGTHFVAVNPRGTTKECASCGVSTDKPLWVREHSCPACGFEADRDANAAWNILSRGLQDVGVGHSESTPVETALPVDTPVSAKRVVEAGS
PTLKERTASAVSE

 

Blast result :
Comments
ISHbo4 is 98% aa (ORFA : the transposase) similar to ISHmu6 and 97% aa ( ORFB) similar to ISHma12.
References
1] Pfeiffer F. (2013) Direct submission.
2] Lucas,S., Copeland,A., Lapidus,A., Glavina del Rio,T., Dalin,E., Tice,H., Bruce,D., Goodwin,L., Pitluck,S., Kyrpides,N., Mavromatis,K., Mikhailova,N., Anderson,I., Brettin,T., Detter,J.C., Han,C., Larimer,F., Land,M., Hauser,L., Markowitz,V., Cheng,J.-F., Hugenholtz,P., Woyke,T., Wu,D., Tindal,B., Klenk,H.-P. and Eisen,J.A. (2009) Direct submission GenBank.