ISfinder

ISKpn26

Family IS5
Group IS5

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
NC_016845	ND	Klebsiella pneumoniae	Klebsiella pneumoniae pK29 Klebsiella oxytoca E718 pKOX R1 Klebsiella pneumoniae subsp. pneumoniae MGH 78578 Klebsiella pneumoniae subsp. pneumoniae HS11286 Klebsiella pneumoniae subsp. pneumoniae MGH 78578 pKPN3

DNA section

IS Length : 1196 bp

Ends

IR Length : 17/24

IRL : GGAAGGTGCGAACAAGTTCCTGATATGAGATCATCATATTCATCCGGAGC
IRR : GGAAAGTGCGAATAAGCAGGTCATTTCTTCCCAAGCTGACTCGTTGATTA

Insertion site

Left flank	Direct repeat	Right flank	DR Length
ACTACAATTT	CTAA	TAGTTTCCTG	4
ATTGACTTAG		CTGTCATCGG	0
AATCATATAA	TTAG	CTATAATGAT	4
ACTGCATTAG		TTAACTCATT	0
CTTAATTAAA	TTAG	TCCGTTTCTC	4

DNA sequence

GGAAGGTGCGAACAAGTTCCTGATATGAGATCATCATATTCATCCGGAGCGCATCCCAGAGGGACATCATGAGCCATCAACTCACCTTCGCCGATAGTGA
ATTCAGCACTAAGCGCCGTCAGACCCGAAAAGAGATTTTCCTCTCCCGCATGGAGCAGATTCTGCCATGGCAGAATATGACCGCTGTCATCGAGCCGTTT
TATCCCAAGGCGGGCAATGGCCGACGGCCCTATCCGCTGGAGACCATGCTGCGTATTCACTGCATGCAGCATTGGTACAACCTGAGCGACGGTGCCATGG
AAGATGCCCTGTACGAAATCGCCTCCATGCGCCTGTTTGCCCGATTATCCCTGGATAGCGCCCTGCCGGATCGCACCACCATCATGAATTTCCGCCACCT
GCTCGAGCAGCATCAACTGGCCCGTCAATTGTTCAAGACCATCAATCGCTGGCTGGCCGAAGCAGGCGTCATGATGACCCAAGGCACTTTGGTGGATGCC
ACCATCATTGAGGCACCCAGCTCTACCAAGAACAAAGAGCAGCAACGCGATCCGGAGATGCATCAGACCAAGAAAGGCAATCAGTGGCACTTTGGCATGA
AGGCCCACATTGGTGTCGATGCCAAGAGTGGCCTGACCCACAGCCTGGTCACCACCGCGGCCAACGAGCATGACCTCAATCAGCTGGGTAATCTGCTGCA
TGGAGAGGAGCAATTTGTCTCAGCCGATGCCGGCTACCAAGGAGCGCCACAGCGCGAGGAGCTGGCCGAGGTGGATGTGGACTGGCTGATCGCCGAGCGT
CCCGGCAAGGTAAAAACCTTGAAGCAGCATCCGCGCAAGAACAAAACGGCCATCAACATCGAATACATGAAAGCCAGCATCCGTGCCAGGGTGGAGCACC
CGTTTCGCATCATCAAGCGGCAGTTCGGCTTCGTGAAAGCCAGATACAAGGGGCTGCTGAAAAACGATAACCAACTGGCGATGTTATTCACCCTGGCCAA
CCTGTTTCGGGTGGACCAAATGATACGTCAGTGGGAGAGATCTCAGTAAAAACCGGAAATAACGCCAGAAATGGTGGAAAAAATAGCCTAAATAGGCTGA
TTCGATGTGTTTGCGGGAAAAAAATCGGCCCAGATCCGCGAAATTTTAATCAACGAGTCAGCTTGGGAAGAAATGACCTGCTTATTCGCACTTTCC

Protein section

ORF number : 1

ORF 1

Length		Begin	End	Strand	Fusion ORF
981 bp	326 aa	69	1049	+	No

ORF function : Transposase
Chemistry : DDE

ORF sequence :

MSHQLTFADSEFSTKRRQTRKEIFLSRMEQILPWQNMTAVIEPFYPKAGNGRRPYPLETMLRIHCMQHWYNLSDGAMEDALYEIASMRLFARLSLDSALP
DRTTIMNFRHLLEQHQLARQLFKTINRWLAEAGVMMTQGTLVDATIIEAPSSTKNKEQQRDPEMHQTKKGNQWHFGMKAHIGVDAKSGLTHSLVTTAANE
HDLNQLGNLLHGEEQFVSADAGYQGAPQREELAEVDVDWLIAERPGKVKTLKQHPRKNKTAINIEYMKASIRARVEHPFRIIKRQFGFVKARYKGLLKND
NQLAMLFTLANLFRVDQMIRQWERSQ

Blast result :

Comments

ISKpn26 is 99% aa similar to IS5 but only 91% at DNA level.

References

1] ISfinder annotation (2014)
2] Liu,P., Li,P., Jiang,X., Bi,D., Xie,Y., Tai,C., Deng,Z.,
Rajakumar,K. and Ou,H.Y. (2012) J. Bacteriol. 194 (7), 1841-1842.