ISKpn27

  • Family IS481
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
NC_016846 ND Klebsiella pneumoniae
Klebsiella pneumoniae pKP048
Klebsiella pneumoniae subsp. pneumoniae HS11286 pKPHS2
DNA section
IS Length : 1080 bp

Ends


IR Length : 17/29

IRL : TGTCTGGACTCGTGGGATCATGTACCCATGCGTAGCTGGCCGCTCTTCAA
IRR : TGTCAAGACCCGGCTGGTTATACACGCGTTTCCTGAACAATTCAGGGCGT

Insertion site


Left flankDirect repeatRight flankDR Length
CGCCTATTTTTATAGGTTAATGTCAT6

DNA sequence

TGTCTGGACTCGTGGGATCATGTACCCATGCGTAGCTGGCCGCTCTTCAAGGCCAGACACGTTTTGCGTACACTGTTCCAATCCGACTCTTCACTGGCAA
CTCGATGACCCAGGCACTGCACAGCCAAGCCCGTACTACCCACCTGATCCGTGAGGAAATCAGGAACTCGACGCTCCCGCAGGCCGAACTGGCCAGGATG
TACAACGTCACCCGCCAAACCATCCGAAAGTGGCAAAACCGCGAGTCTCCTGAAGACAAGTCGCATGCGCCGAACAAGATGTACACGACGCTCACGCCCG
AGCAGGAGCTCATCGTGGTGGAGCTGCGCAAGACGTTGCTGCTGCCCACGGACGACCTGCTGGCGGTCACGCGCGAGTTCATCAATCCAGCCGTCTCGCG
TGCCGGCCTGGGACGTTGCCTGCGCCGCCACGGTGTCTCGGATCTACGTAACCTGGTCGAGCAGGAAGGCACTGCGCCCGCCACGAAAAAGACCTTCAAG
GACTACGAGCCGGGCTTTGTGCACATCGACATCAAGTACCTGCCGCAGATGCCCGACGAGACGGCAAGGCGCTATCTCTTCGTTGCCATCGACCGTGCTA
CGCGCTGGGTCTTCATCGAGCTCTATGCCGACCAGACCGATGGCAGCAGTGGCGACTTCCTCAACAAAGTCCAGCAAGCCTGTCCCGTCAAGATCGTCAA
GCTTCTGACCGACAACGGCAGCCAGTTCACCGACCGCTTCACGGCTGGCGGCAAGAAGAAGGAACCCAGCGGCACACACGTGTTCGACCGCCTGTGCAAG
CAGCTCGGCATCGAGCACCGGCTCATCCCGCCTCGTCATCCGCAGACCAACGGCATGGTGGAGCGCTTCAACGGTCGTATCAGCGACATCGTCAACCAGA
CCCGTTTTGGTTCAGCTGCCGAACTGGAATCGACGCTGCGCAATTACGTCAAGATCTACAACCACAGCATTCCGCAACGCGCGCTCCAACACAAAACACC
CGTTCAGGCGCTCAAGGAATGGCATGAAAAACGCCCTGAATTGTTCAGGAAACGCGTGTATAACCAGCCGGGTCTTGACA
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
981 bp326 aa1051085+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MTQALHSQARTTHLIREEIRNSTLPQAELARMYNVTRQTIRKWQNRESPEDKSHAPNKMYTTLTPEQELIVVELRKTLLLPTDDLLAVTREFINPAVSRA
GLGRCLRRHGVSDLRNLVEQEGTAPATKKTFKDYEPGFVHIDIKYLPQMPDETARRYLFVAIDRATRWVFIELYADQTDGSSGDFLNKVQQACPVKIVKL
LTDNGSQFTDRFTAGGKKKEPSGTHVFDRLCKQLGIEHRLIPPRHPQTNGMVERFNGRISDIVNQTRFGSAAELESTLRNYVKIYNHSIPQRALQHKTPV
QALKEWHEKRPELFRKRVYNQPGLDI

 

Blast result :
Comments
ISKpn27 is 73% aa similar to ISAisp3. The STOP codon of the transposase is done by the target.
References
1] ISfinder annotation (2014)
2] Ou,H.-Y., Jiang,X., Liu,P. and Li,P. (2011) Direct submission GenBank.