ISAhy2

  • Family IS630
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
FM877486 ND Aeromonas hydrophila
Aeromonas media WS
Aeromonas sp. GK7
Aeromonas hydrophila F181
Aeromonas punctata HGB5 plasmid pFBAOT6
Aeromonas caviae HGB5 plasmid pFBAOT6
DNA section
IS Length : 1158 bp

Ends


IR Length : 16/19

IRL : AATAGCTGTTCCGAATAGTAGATAACCGGAGGAAGGGAACTCAGTCCAGT
IRR : AATAACTGATCCCAATAGTGCTACACCTTCGCTGTGCCATAGCCATTCCC

Insertion site


Left flankDirect repeatRight flankDR Length
TTTTGGCCTCTAGCCCTTGCGT2
CGCTGAGGTCTAGGCTATGGAG2

DNA sequence

AATAGCTGTTCCGAATAGTAGATAACCGGAGGAAGGGAACTCAGTCCAGTTTGTGCGATCTGATTAGTCGCTAAACAAAACAAGCCACAGCCAAAGGACT
GAGTTGATGCCTATCCTACCACCTCTGCCACGACCACAACGGCGCCGCATTCACAAAATCATCCATGCCACCCGTGATAAAGGACATGCTCGTCGCTTGA
TGGCCATCTTGCTGTTACATGAAGGACGCACGATCACCGATGTTCATCACCTTACCGGCGCTGCGCGCTCGACCATCGGTCGTTGGCTTCGGTGGTATCG
AGATGAAGGCATAGATGCGCTAGAAGCCTTACCGGCTGGCCGTGCCCCAGTCTTACCTATCGCCAAGATTGTCACCTTACTGGTGCTGCTCATGCAGTTT
TCTCCGCAAGATTTTGGTTATCAGCGCAGCCGTTGGTGTACTGAACTATTGGCTATAAAAATCAATCGGTTAACAGGGCTCAATGAGGCAGCCTCCACGC
TCCGCCGATGGTTACCTCGTATGGGCATTGTCTGGCGCAGGCCCGTGCCGACCTTGCGGATAAAAGACCCAGCCTATCAGGAAAAAATGGCGAATATCGA
CGCCGCGCTGGCCCGCTGTGATGCCGATAATCCGGTGTTTTATGAGGATGAAGTCGATATAGACCTCAATCCCAAATTAGGAGCCGACTGGATGTTCAGA
GCGCAGCAAAAGCGGGTTGTCACACCGGGGCAGAATGCCAAGCATTATCTGGCAGGCGTTCTCCACGCAGGCAACGGAAGGGTACTTTACGTCAGCGGGA
TCAAGAAAAACTCATCGCTGTTTATTGCCATGCTGGAAAAGCTGCGCCACCACTACCGACGAGCCAAAACCATCGCGTTGATCCTCGATAACTACATCAT
TCACAAAAGCAAACAGACCGAGCGATGGTTGAAGAAGAATCCCAAGTTCTGCCTGGTGTTCCAGCCAGTTTACAGCCCTTGGGTTAATCACATAGAACGG
CTATGGCACAAGTTACATGAAACCATCACGCGCAATCATCAATGCAGAGGGATGGCAAACCTGCTGGCCCGAGTGAAACACTTCATGGATACCGTGTCTC
CTTTCCCGGGGAATGGCTATGGCACAGCGAAGGTGTAGCACTATTGGGATCAGTTATT
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
1032 bp343 aa1071138+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MPILPPLPRPQRRRIHKIIHATRDKGHARRLMAILLLHEGRTITDVHHLTGAARSTIGRWLRWYRDEGIDALEALPAGRAPVLPIAKIVTLLVLLMQFSP
QDFGYQRSRWCTELLAIKINRLTGLNEAASTLRRWLPRMGIVWRRPVPTLRIKDPAYQEKMANIDAALARCDADNPVFYEDEVDIDLNPKLGADWMFRAQ
QKRVVTPGQNAKHYLAGVLHAGNGRVLYVSGIKKNSSLFIAMLEKLRHHYRRAKTIALILDNYIIHKSKQTERWLKKNPKFCLVFQPVYSPWVNHIERLW
HKLHETITRNHQCRGMANLLARVKHFMDTVSPFPGNGYGTAKV

 

Blast result :
Comments
ISAhy2 is 76% aa similar to ISSod16.
This IS disrupts a coding sequence itself located within a gene cassette of a class 1 integron
References
1] Thomas Jové (2014) Direct submission
2] Kadlec,K., von Czapiewski,E., Kaspar,H., Steinacker,U., Wallmann,J. and Schwarz,S. (2008) Direct submission GenBank.