ISAs6

  • Family IS3
  • Group IS3
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
CP000644 ND Aeromonas salmonicida
Aeromonas salmonicida subsp. salmonicida A449
DNA section
IS Length : 1239 bp

Ends


IR Length : 21/26

IRL : TGTAGTGGTCTAATCATCCCGGACACCATTTTAGGTGGTAGAGTCGCCAC
IRR : TGTAGTGGTCAACTAAATCCGGACACTTGATTAGGCCGATCCTCGGCCTC

Insertion site


Left flankDirect repeatRight flankDR Length
CCACCTGAACCTCGCACCGA0
TCGCTCAGGGCGGTGTGTGG0
CCCGGCTGTTATTGTCGCCG0

DNA sequence

TGTAGTGGTCTAATCATCCCGGACACCATTTTAGGTGGTAGAGTCGCCACCATAATCTGAGGTGTTCAATGACAAGATTGCGTCGCTCATTTACTGCCGA
GTTCAAACTGGAAGCCGCATCCCTGGTGGTCGACCAGGGGTATTCAGTCCCCGAAGCCAGCCGTTCGCTGGATGTCGGTGAAACCGTACTTCGCCGCTGG
GTTCAGCAGCTTCAATCCGAACGCTGTGGCACTACCCCAATCAGCAAAGCGCTGACTCCGGAGCAGCAGAAAATCCAGGAGCTGGAAGCCCGTATCAACC
GGCTCGAGCGCGAAAAGGACATACTAAAAAAGGCTACTGCTCTCTTGATGGCGGACGAGTTCACACGTACGCGCTGATAGACCGATTAAGGGAGCAAGCC
CCCATCACCTTGGTCTGCTGCGCTTTTGATGTTCCCAAGTCCTGTTTTTACGACTATTTAGCCCGCAAGCGGACGATTAACCGTGAGCGGATGAAACAAC
GAAGCGAGTTGCGCCGTTTGTTCAAAGAGAGCCGGGATTCCGCAGGCAGCCGCGCCTTGATGTCGATGATGCGAGAGCTGGGGTATCAGATTGGCCGATT
CAAAGTCCGTAACCTGATGAAGGAAGCTGGGCTGGCGTCCAAACAGCCGGGCGCACACCGTTATAAGGTTGCATGCTCTGAACGACCGGATATTCCGAAT
CTGCTGGCCCGCGAATTTGATGTTCCGCAACCCAATCAGGTGTGGTGTGGCGATATCACCTACGTCTGGACAAGTGCTCGTTGGCATTACTTAGCAGTGG
TGCTTGACCTTCATACCCGGCGAGTCGTGGGCTGGGCGATGTCTGACAAGCCTGATGCAGAGCTGGCAATCAAAGCACTGGAGATGGCCTATCAGCAGCG
GGGTTGCCCATCCGGTGTGCTGTTTCACTCCGACCAGGGCAGTCAATATGGCAGCCGGGCATTTCGGCAACGGTTGTGGCGCTATCGCATGACCCAGAGT
ATGAGCCGGCGTGGAAACTGCTGGGATAACGCCCCGATGGAGCGGTTGTTCAGAAGCCTGAAGTCAGAGTGGCTTCCTGCGACGGGATACGTGAGCCTGC
GGGAAGCGAAGCGGGATATCAGTTATTACCTGATGGATTACTACAACTGGCGGCGTCCACATCAACACAATGACGGGATACCGCCAGCAGAGGCCGAGGA
TCGGCCTAATCAAGTGTCCGGATTTAGTTGACCACTACA
Recoding section
  • Recoding by frameshift
  • Frame
  • Type
  • Experimentally demonstrated

Stimulators :

  • Shine-Dalgarno sequence :
  • Secondary structure :

Recoding motif :

Protein section
ORF number : 3

 

ORF 1
LengthBeginEndStrandFusion ORF
309 bp102 aa69377+No
ORF function : Transposase
Description : First part of the transposase

ORF sequence :

MTRLRRSFTAEFKLEAASLVVDQGYSVPEASRSLDVGETVLRRWVQQLQSERCGTTPISKALTPEQQKIQELEARINRLEREKDILKKATALLMADEFTR
TR

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
1050 bp349 aa1821231+No
ORF function : Transposase
Description : Second part of the transposase

ORF sequence :

NRTSPLGSAASIRTLWHYPNQQSADSGAAENPGAGSPYQPARARKGHTKKGYCSLDGGRVHTYALIDRLREQAPITLVCCAFDVPKSCFYDYLARKRTIN
RERMKQRSELRRLFKESRDSAGSRALMSMMRELGYQIGRFKVRNLMKEAGLASKQPGAHRYKVACSERPDIPNLLAREFDVPQPNQVWCGDITYVWTSAR
WHYLAVVLDLHTRRVVGWAMSDKPDAELAIKALEMAYQQRGCPSGVLFHSDQGSQYGSRAFRQRLWRYRMTQSMSRRGNCWDNAPMERLFRSLKSEWLPA
TGYVSLREAKRDISYYLMDYYNWRRPHQHNDGIPPAEAEDRPNQVSGFS

 

Blast result :
ORF 3
LengthBeginEndStrandFusion ORF
1163 bp387 aa691231+Yes
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MTRLRRSFTAEFKLEAASLVVDQGYSVPEASRSLDVGETVLRRWVQQLQSERCGTTPISKALTPEQQKIQELEARINRLEREKDILKKGYCSLDGGRVHT
YALIDRLREQAPITLVCCAFDVPKSCFYDYLARKRTINRERMKQRSELRRLFKESRDSAGSRALMSMMRELGYQIGRFKVRNLMKEAGLASKQPGAHRYK
VACSERPDIPNLLAREFDVPQPNQVWCGDITYVWTSARWHYLAVVLDLHTRRVVGWAMSDKPDAELAIKALEMAYQQRGCPSGVLFHSDQGSQYGSRAFR
QRLWRYRMTQSMSRRGNCWDNAPMERLFRSLKSEWLPATGYVSLREAKRDISYYLMDYYNWRRPHQHNDGIPPAEAEDRPNQVSGFS

 

Blast result :
Comments
The third ORF is a putative ORFAB transposase reconstructed in silico by a possible -1 frameshift.
ISAs6 is 81% aa similar to IS222.
References
1] Reith,M.E., Singh,R.K., Curtis,B., Boyd,J., Bouevitch,A., Kimball,J., Munholland,J., Murphy,C., Sarty,D., Williams,J., Nash,J., Johnson,S. and Brown,L. (2007) Direct submission to GenBank