ISNamo21

  • Family IS200/IS605
  • Group IS1341
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
HF582854 ND Natronomonas moolapensis
Natronomonas moolapensis 8.8.11
DNA section
IS Length : 1736 bp

Ends


Left end : AGCGGTAGCGAGGCGAAAGCCCACGGCTTTAGCCGTGGGATGAAGCCGACAACAGGGAAGCTAACCACGTCCGATGGCCGGCGTGGATTTCCCACCGCCT II struct. : Yes
Right end : TGGTATCAGCCCCGCTACGACGTGCTCATCTCCAGTGAATGGGCAGCACCGAACACGTACTATCCGGGGTTCGACCTCGACGACGTCGAGGCCGGCAACT II struct. : Yes

Insertion site


Left flankLE cleavage siteRight flankRE cleavage site
GATGAACTACGATTACTGGTATCAGCCCTCAA

DNA sequence

AGCGGTAGCGAGGCGAAAGCCCACGGCTTTAGCCGTGGGATGAAGCCGACAACAGGGAAGCTAACCACGTCCGATGGCCGGCGTGGATTTCCCACCGCCT
ACACCGCGTCGTCACCTTCCATTCGCCGCGCTCCCTGCAATAGCAGTCCCTTCCATGTCAACCCGTGTTTCTTCTTGATCCGCTTCAACTCCTCGTACTG
CTCGTCGTCGTCCATCTCGACATATATCTTATTCGTCTCACCCATACTTAACCCATACATACCCAACTTATATATAGGTTCAGGAGCTACACGTTGGTAC
AGCGATGAAGCGCGTCAACGAGTTCGAGATACGCCCACGCACCCCCGAACAGCGTGAGGCACTCCTCGAACTCCTTGACGCGTCAGCCGCACTCTGGAAC
GAACTCACCTACAACCGCAGACAAGCCTTCTTCGACGACAACTCCGTGTGGAACGCCACCAACCCCAGCGACCGCTACAAAAACGTACTCGGGAGCGCGA
CCGCCCAACAAATCCCGCGCAAGAACAGCGAGGCGTGGCGGTCGTTCTTCAGCCTCAACGAAAAATACTACAGCGGCAAGCGCGACGAGAAACCCAGCCC
GCCCGGCTACTGGGGCAACGAGGAGGATGGCCGAGAGTTGCGGACGTACATCCGCAACGACCAATACACCCTCCAGTGGGGCGACCGCAGCCGCCTCGAA
ATCCCCGTCGGGAAGGCGTTGAAAGACGAGTACGCCATCACCGGCAGACTCCGCCTCGAAGCCTGCGGCGACCCGAAGTGGGATGGGGAACAGGGTCGGT
TGGAACTGGTGTACGACAAGACTTGCGACACGTTCAGGGCACTTCAACCCGTCACCGTGCCTGATTCTCGACGGGATTCACGGAAAATCGAAGATTTTCC
TGAAGTCGAAAACGCGCAGCGTTTTCGGAATTCACCACTGGCCTCTCACGAGGCCGCCTTGGACGTGGGCGCGAACAACCTCGTCGCCTGCACCACCACA
ACCGGCCAGCAGTACCTCTACGAGGGCCGCGAACTGTTCGCCCATTTCCGTGACACTACCGAAGAGATTGCCCACTACCAGTCGATGCTTGATAATCAAC
GACGAACAAGCAAGCGCATCGACAGTTTGTACCGCCAACGCACTGAACGACGAAATCACGCGCAGGACGCGCTGGTGCGCGATCTCATCGAACGATTGCA
CGACGAAGGTGTTTGTCGGGTGTACGTCGGCGATCTCGAAGACGTGCTGGAGACGCATTGGAAATGCGTCGTGAACGAGAAGACGCACAACTTCTGGGCG
TTCTCGCAGTTCATCGACCGCCTCAAGTGTGTCTGCGAGGAGTACGGTATTGAGGTGGTTGAGAAGTCGGAAGCGTTGACGACCCAAGAGTGTCCGGAGT
GCGGCGAGCGAGAGGAGACGCATCGGAACGGAGACGTATTCCGGTGCGGTGCGTGTGGGTTCGAGGGACACGCGGATTTGAAGGCGAGCAGGGTGTTCCT
TGACCGTGAGGCCGGTGGTGAAGTTGGGCCGATGGCCCGGCCCGTGCGCCTCGAGTGGGACAATCACGAGTGGCGGCCTGCCACAACCGCCCCGCCTCTT
CGGCGGGCAAATCCCAAAGAGGATCGCACAGACCGGAGTACCTGCCGCGAGGTGGGGAAAGTTGCCTCCGTGGGATCGTCATAGCCGAAATCCCCACCGG
AGGAATCCCACCCCTTCAGGGGTGGGAGGAAGTCAATGGTATCAGCCCCGCTACGACGTGCTCATCTCCAGTGAATGGGCAGCACCGAACACGTACTATC
CGGGGTTCGACCTCGACGACGTCGAGGCCGGCAACT
Protein section
ORF number : 2

 

ORF 1
LengthBeginEndStrandFusion ORF
147 bp48 aa24599-No
ORF function : Passenger Gene
Annotation : Hypothetical proteinDescription :

ORF sequence :

MGETNKIYVEMDDDEQYEELKRIKKKHGLTWKGLLLQGARRMEGDDAV

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
1380 bp459 aa3051684+No
ORF function : Accessory Gene
AG : TnpB

ORF sequence :

MKRVNEFEIRPRTPEQREALLELLDASAALWNELTYNRRQAFFDDNSVWNATNPSDRYKNVLGSATAQQIPRKNSEAWRSFFSLNEKYYSGKRDEKPSPP
GYWGNEEDGRELRTYIRNDQYTLQWGDRSRLEIPVGKALKDEYAITGRLRLEACGDPKWDGEQGRLELVYDKTCDTFRALQPVTVPDSRRDSRKIEDFPE
VENAQRFRNSPLASHEAALDVGANNLVACTTTTGQQYLYEGRELFAHFRDTTEEIAHYQSMLDNQRRTSKRIDSLYRQRTERRNHAQDALVRDLIERLHD
EGVCRVYVGDLEDVLETHWKCVVNEKTHNFWAFSQFIDRLKCVCEEYGIEVVEKSEALTTQECPECGEREETHRNGDVFRCGACGFEGHADLKASRVFLD
REAGGEVGPMARPVRLEWDNHEWRPATTAPPLRRANPKEDRTDRSTCREVGKVASVGSS

 

Blast result :
Comments
ISNamo21 is 73% aa (TnpB) similar to ISHbo5.
The first ORF is a passenger gene annotated as hypothetical protein.
References
1] Pfeiffer F. (2013) Direct submission.
2] Dyall-Smith, M.L., Pfeiffer, F., Oberwinkler, T., Klee, K., Rampp, M., Palm, P., Gross, K., Schuster, S.C., Oesterhelt, D.(2013) Genome Announc. 1: e0009513