ISAjo2

  • Family IS1202
  • Group ISAba32
MGE type ISRelated element(s) :
Isoform Synonym(s) ISAba71
Accession numberTranspositionOriginHost
ND Acinetobacter johnsonii
Acinetobacter baumannii
Acinetobacter johnsonii
Acinetobacter baumannii S30 plasmid pS30-1
DNA section
IS Length : 1482 bp

Ends


IR Length : 20/24

IRL : TGTAACTCCAAAATAGAAATGTCCGGTTTCTCCAAAGTAAAAATGTCCGC
IRR : TGTTATTCCTAAGTAGAAATGTCCTACCTAATAACCAAATAGAAATGTCC

Insertion site


Left flankDirect repeatRight flankDR Length
TATACGAATCTAAAAATGGGAGCCTT6
GAAACGAGAGAAAAATGGGA0
TATACGAAACGAGAGAGTGTTAGAA5
TATACGAAGTACACTTGTATATTAA5
GCAATACACTAATCTTGTTA0
GAAGTAACCTGCATATAAAA0
TATGCGAAATTTGCCTGAATAGCCTT6

DNA sequence

TGTAACTCCAAAATAGAAATGTCCGGTTTCTCCAAAGTAAAAATGTCCGCTTTTAGAATATGCACTTTTGCAATTTTCTTAGCGGACGGTTTGATATGTT
GGTGTCTATGTCGGATAAAGAACTTAAACGATTGTCGGTCTTGCAAGAAATCTGTGATCAACGCATAACCCAGTCCCAAGCTGCTCAGCTACTTCATATT
TCAGAACGTCAGATCAGACGTTTATTGCAAAAATACAAAGCTCAAGGCCCAGCTGCATTAGCACATGCTGCACGTGGCCAAATCAGCAATTCCAGGCTTC
CTGAAGAGCTCAGACTCAAGTGCCTCAATATTGTTTCGGATCAACTGCATGGTTTCGGACCCACTTTAGCGCATGAAAAGCTCACCACTGTTCATGGATT
CGATATTTCAGTGGAAACACTGCGTTCCTGGATGATTGCAGCCGATCTGTGGATTCCTCGCGCCAAGCGCCTGAAACGCCCGTATCAGCCTCGTTATAAC
CGAGATTGTTATGGTGAACTGATTCAAATCGATGGCTCACACCATGACTGGTTTGAAGGACGCGCTGCTAAGTGCTGTCTGCTGGTATTTATCGATGATG
CCACAGGAAAATTACAGCATTTACGTTTCTGTGAGTCGGAATCAGCATTTGACTATATGATTTCAACACGTTTGTATGTTGAGCAGCATGGTAAGCCGTT
GGCGTTTTACAGTGATAAACATTCAGTCTTCAGGGTGAATCAAAGCAGCAAGAAAGACACCAAGATTACCCAGTTTGGACGCGTACTCAGTACCCTGAAT
ATCGATATCATCTTCGCCAATTCACCACAGGCCAAAGGCCGTGTGGAACGTGCCAACAGAACCCTTCAGGACCGTCTGATCAAGGAGATGCGTCTGGAAG
GTATCAGCTCGATTGAGCAAGCTAATGCCTGGCTACCCTGCTTCATTGAGCAGTTCAATCAGAAGTTCGCCAAGATGGCTTTTAATCCTAAGGATCTACA
TCGGCCTATCACTGAAACAGCCGAAGAATTAGATGATGTTTTTACCTGGCGTGAACCCCGCAGAGTCACGAACAGCCTGACGATTACTTATGATAAATGC
GTATATTTACTGGAAAATACCGAAGAAAATAAAAGGTTGATCGGCAAGTATCTTGAGTTTCTGGAATACCCGGATGGCACGGTAGCCATTGAACATCAAG
GAAGAAAAATCAATTACAGCATCTTCGATAAATTAAGTCAGCTCAACCAGCGAGAGGTTGTTGAGAATAAACGTTTAGGTGCTGTTCTCAATCATATTCA
ACAACAGCACGAAGAATTAGAACAGCAAAACAAACGCAATCGTTCTCAAAAGATGCCAAGCAGACGTGCACAGAAAATAGCAATCAAAGAACGAAATCTA
AATCCTGTGCTTGACTTGGAATTGTCCATATAGGACATTTCTATTTGGTTATTAGGTAGGACATTTCTACTTAGGAATAACA
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
1326 bp441 aa1081433+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MSDKELKRLSVLQEICDQRITQSQAAQLLHISERQIRRLLQKYKAQGPAALAHAARGQISNSRLPEELRLKCLNIVSDQLHGFGPTLAHEKLTTVHGFDI
SVETLRSWMIAADLWIPRAKRLKRPYQPRYNRDCYGELIQIDGSHHDWFEGRAAKCCLLVFIDDATGKLQHLRFCESESAFDYMISTRLYVEQHGKPLAF
YSDKHSVFRVNQSSKKDTKITQFGRVLSTLNIDIIFANSPQAKGRVERANRTLQDRLIKEMRLEGISSIEQANAWLPCFIEQFNQKFAKMAFNPKDLHRP
ITETAEELDDVFTWREPRRVTNSLTITYDKCVYLLENTEENKRLIGKYLEFLEYPDGTVAIEHQGRKINYSIFDKLSQLNQREVVENKRLGAVLNHIQQQ
HEELEQQNKRNRSQKMPSRRAQKIAIKERNLNPVLDLELSI

 

Blast result :
Comments
ISAjo2 is 64% aa similar to ISMex6.
References
1] Zhiyong ZONG (2015) Direct submission to ISfinder
2] Blackwell G. (2016) Direct submission to ISfinder