ISHti6

  • Family IS630
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
HF571521 ND Halorhabdus tiamatea
Halorhabdus tiamatea SARL4B plasmid pHTIA
DNA section
IS Length : 1154 bp

Ends


IR Length : 8/13

IRL : GTGGTTCACAGAAGATTTAGGTGTACTCTGAGTTGGTAGGGTAATGACGG
IRR : GTGACTAACGCAACTTCTGAATGAATCCGTCGAGGTGATTTTCGATCCAA

Insertion site


Left flankDirect repeatRight flankDR Length
CCCCGCCCTGTAAAGGGCGGGG2

DNA sequence

GTGGTTCACAGAAGATTTAGGTGTACTCTGAGTTGGTAGGGTAATGACGGGACGAGAGAAAGAGATCGTCCGTCACCTCAGTGAGGACGATCTCGACCGC
TTGCTCACCCAGACAGATGACGAGAAGGTGAGCAAGCGGCTCACCTTCATCAAACGGCTGTACAAGGGTGCAACACTCGAAGACGCAGCCGACGATGTCG
GGATGTCCCAATCAACCGGCAGTCGCTGGGCCAGACTCTGGAACAAGGGTGGGCTCGGACTTCTCACACCGAGCTTCGGGGGCGGTCGGCCCCCGAAGCT
CGACGAGGAACAACGAGAGCGTCTCTTAGACCTGCTCGAAGAGGGCGAACCCTGGAAGAAACAGGAGATACAGCACCTCATCAACGAAGAGTTCGATGTC
GAGTTCCATCCGAATTATCTGCCCCGACTTCTCGATGATCTCGGCCTCTCGTACGCGATTCCGCGTACGGAGAGGCCGGACCGACCAGAGAACGCCGACG
AAATCCTCGACGAACGCGTTGCCGACGCGTTCGCCGAGGATGATGCAGACGAGCCACACAATAAACAGCCAGAAGACGATAGTGACGAAGAATGGACGCT
CGACGATGATGTGCGAACGGATGGCGGAACGACGGTTGGTTTCTTCGACGTTTCGCACCCGCAGCCGTGGGACAATTCTCAGCGCCTCTACACAGTGACC
GAGCCGACGATCACCCGGCCGCTGGTGAAAATCGACACACCAGCGGCCGGGTTCTATGCACTCAACGGTGAAAGCCTCCTTCAGTTCCCACCGAATCAGG
AGAAAGAAGAGATCTGTGCGTGTCTCGAAGCGATCCGCGAGCAGAATCCGGGCGAGCGGATTCTGCTCGTCTTGGACAACTTCTCGTCACATATCTGTAC
GCACACGCGCAAGCGAGCCCATGAGATTGGGATTGACCTCGTCTTTCTCCCAGTGGGCTCACCACATCTCAACCCAATCGAGCCCGTCTGGAAGAGTCTC
AAATGGGAATCGTCGCCCCTGATCGTGGAGGGCGCGGCTGAATACCGCGCCCTCCTCGATGAGATCTTCACGGCATTGACCGACCAGCTGAGTTTCGCAG
GCTCTTGGATCGAAAATCACCTCGACGGATTCATTCAGAAGTTGCGTTAGTCAC
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
1107 bp368 aa441150+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MTGREKEIVRHLSEDDLDRLLTQTDDEKVSKRLTFIKRLYKGATLEDAADDVGMSQSTGSRWARLWNKGGLGLLTPSFGGGRPPKLDEEQRERLLDLLEE
GEPWKKQEIQHLINEEFDVEFHPNYLPRLLDDLGLSYAIPRTERPDRPENADEILDERVADAFAEDDADEPHNKQPEDDSDEEWTLDDDVRTDGGTTVGF
FDVSHPQPWDNSQRLYTVTEPTITRPLVKIDTPAAGFYALNGESLLQFPPNQEKEEICACLEAIREQNPGERILLVLDNFSSHICTHTRKRAHEIGIDLV
FLPVGSPHLNPIEPVWKSLKWESSPLIVEGAAEYRALLDEIFTALTDQLSFAGSWIENHLDGFIQKLR

 

Blast result :
Comments
ISHti6 is 86% aa similar to ISNpe13.
References
1] Friedhelm Pfeiffer (2015) Direct submission
2] Werner,J., Ferrer,M., Michel,G., Mann,A.J., Huang,S., Juarez,S., Ciordia,S., Albar,J.P., Alcaide,M., La Cono,V., Yakimov,M.M., Antunes,A., Taborda,M., da Costa,M.S., Hai,T., Glockner,F.O., Golyshina,O.V., Golyshin,P.N. and Teeling,H. (2014) Environ. Microbiol. In press