ISHaha9

  • Family IS200/IS605
  • Group IS1341
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
HE717023 ND Halobacillus halophilus
Halobacillus halophilus DSM 2266
DNA section
IS Length : 1417 bp

Ends


Left end : ACGAAATTAGCAAGAAGTCCCCTTCCTCAAAAATGCGTTAGCATTTTAGGTGGGGGATGAATTGTTTCTTTTTTTGTTTTTCTTTTGATAATAAAAGACT II struct. : Yes
Right end : TGGGGTTAGCTCGGCAAATTTGGTTGGCTACTAGAAGCAACTACGACCCGAGAAGCCCCCACTTCAAATTTCGTTAGTAATTAAGTGGTGGGTAGTTCAC II struct. : Yes

Insertion site


Left flankLE cleavage siteRight flankRE cleavage site
AGCCTACTTTTTTAAGGGTCACAGtcac
AATTATAAAATTTACAAAGCCTTTtcac
ATTTCCACTTTTTATAATGCTAGTtcac

DNA sequence

ACGAAATTAGCAAGAAGTCCCCTTCCTCAAAAATGCGTTAGCATTTTAGGTGGGGGATGAATTGTTTCTTTTTTTGTTTTTCTTTTGATAATAAAAGACT
TTCGAAGAATTTTTTGGTACTATTGAATTACGAACAAACATTCTGTTGAGAGGAGGTAATTTGATATGGTCAAAAAGAACAAAGCTTTCAAATTCAAAAC
AGAACCAAATTTTGAGCAACTAAACTTAATTAAAAGAACCTTCGGTTCTGCCCGCTTTATATTCAACAAAATGCTGGATGACCGCATTTTAGCTTATGAA
GATTACAAAAATGACCAAAGCGAACTGAAGAAAATGAAGTTTCCAACGCCAGCGAAATACAAAAAAGAATATCCTTTTTTGAAAGAAGTGGATAGTCTTG
CTTTGGCCAACACTCAAATGAACCTGGATAAAGCTTATAAGGCTTTTTTTACTGGTCAAAATAAATTCCCAAAACACAAAAGCCGTAAAGCTCGTAAGAG
CTATACGACAAATGTGGTTAACGGAAATATTATGGTCCAGGATGGCTATATCAAATTACCGAAACTCAAATGGGTGAAGTTGAAACAACATAGACAAATA
CCTGAAGGAAGCAAGTTGAAATCGGTCACGGTTTCAATGTCTGCGACAGGTAAAATTTATGTTTCTATTCTTACTGAATACGAAGTTGAACCTAAAGAGG
TTCAACCAGAAAATATTATTGGTCTAGATTATTCTATGAAGGAATTGTTTGTAGCAAACGAAGGCACGAGAGCCAAGTACCCTCGTTTCTACCGTCAATC
AGAGGATAGACTAGCCAAAGAGCAGCGCGTGTTATCACGCAGAAAGAAAGGCTCGAACCGTTGGTTCAAGCAGAAAAATAAAGTAGCGAAGCTACATGAA
AAAGTAGCAAACCAACGCAAAGATTTCTGCCACAAGTGGAGCCGTAATTTAGCTGATAACTTTGATGCCATAGCTATTGAAGACCTTAACATGAAAGATA
TGTCTAAGGCTCTTAAATTCGGCAAAAGCGTGCACGATAACGGATGGGGAATGTTCGCCTCATTTCTTTCTTACAAGCTCGAAGAGCTTGGAAAGAAGCT
TGTCAAGGTCGGCAAATGGTTCCCATCCTCTAAAACGTGCTCCAAATGCGGTGGTATTGATAAACAACTAACAATGAGCATACGAACTTACAAATGCGCT
TGTGGCCACGTTCAAGACCGCGATGTGAACGCGGCTATTAACATAAAAGTAGAAGGAAAAAGGCTCCTTTCGGAGCATCAGTAATCCTAATTTCCATAAT
ATAAACCGTTGGGACAATGGGGTTAGCTCGGCAAATTTGGTTGGCTACTAGAAGCAACTACGACCCGAGAAGCCCCCACTTCAAATTTCGTTAGTAATTA
AGTGGTGGGTAGTTCAC
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
1119 bp372 aa1661284+No
ORF function : Accessory Gene
AG : TnpB

ORF sequence :

MVKKNKAFKFKTEPNFEQLNLIKRTFGSARFIFNKMLDDRILAYEDYKNDQSELKKMKFPTPAKYKKEYPFLKEVDSLALANTQMNLDKAYKAFFTGQNK
FPKHKSRKARKSYTTNVVNGNIMVQDGYIKLPKLKWVKLKQHRQIPEGSKLKSVTVSMSATGKIYVSILTEYEVEPKEVQPENIIGLDYSMKELFVANEG
TRAKYPRFYRQSEDRLAKEQRVLSRRKKGSNRWFKQKNKVAKLHEKVANQRKDFCHKWSRNLADNFDAIAIEDLNMKDMSKALKFGKSVHDNGWGMFASF
LSYKLEELGKKLVKVGKWFPSSKTCSKCGGIDKQLTMSIRTYKCACGHVQDRDVNAAINIKVEGKRLLSEHQ

 

Blast result :
Comments
ISHaha9 is 78% (TnpB) aa similar to ISBth16.
References
1] Pfeiffer, F. (2012) Direct submission.
2] Saum S.H., Pfeiffer F., Palm P., Rampp M., Schuster S.C., Müller V., and Oesterhelt D. (2013) Environ. Microbiol. 15 (5), 1619-1633.