ISNph24

  • Family IS200/IS605
  • Group IS1341
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
CR936257 ND Natronomonas pharaonis
Natronomonas pharaonis DSM 2160
DNA section
IS Length : 1451 bp

Ends


Left end : AGTGTTGCGAGGCGAAAGCCCACGGCTTCAGCCGTGGGATGAAGCCGACAACAGGGAAACGTTCCACGCTCGGGCGCTCGGCTGGATATTCCAGCGTGCT II struct. : Yes
Right end : ACGCTGGCCACGCGAAAGCGTATTTGAGCCGCCGAGGAAACGCGCAACCCTAATATCCCCATCGGGGAATCCCACGGCTTCAGCCGTGGGAGGAGGTCAA II struct. : Yes

Insertion site


Left flankLE cleavage siteRight flankRE cleavage site
TATCGTCTATCATTACATTCTAACTAATtcaa

DNA sequence

AGTGTTGCGAGGCGAAAGCCCACGGCTTCAGCCGTGGGATGAAGCCGACAACAGGGAAACGTTCCACGCTCGGGCGCTCGGCTGGATATTCCAGCGTGCT
GAACCATCAACTTTTACAAATCTGCAACAGTACGTTACACGTGTAATGGTCGAGGTGAGTGTGGAAACCACGTTCCGCAATCCCAGCCGCTCGCGCCGCC
AAGAGTGGCAACGAGCCACCCACATCCTCCGCGAGTGCAAACAATGGCTCGTGGACGGCTGGGAGGACGACTCGCTCGCCTCGTCCGTGACGACTGGCGA
CATCGACAACCCACTCTACTCAGCACTCCAAAACCAAGCCATCCGAAACGCGAAAAGCGACTACGCCGACGAATCTATCGAGTACACGGGCGAACAGCCC
ATCGAGGTCAACAACCAGAACTGGGAAATCCACACGACTGAGAACGATTCTGTCGTCATCGGATTCCCGTGTATCAGTGATTGGTGGTACACGCCAATCC
ACGTCCACGAAAGTATTGAAGAACCCGTCCAAGCTCTGCTCGATGGTGACGCTGAGAAGACAAAACTCAGCGTGTGGCGAGAAGGCGGCGACTGGCATTG
CTCGTTCACAGTCGAATACGACGAGCAACAGGATGGCGAGGAAACACCCATTGGTGTTGACGTTGGTCACAACTACATTCTCGCGGCCACGCCAGACAAT
GCGAGTGCTGAGTCGTTCCTTGTGAGTGGCAAGGAACACAAGTTTGTGCGGCGGTACTACCGTTCCCTGCGTGACTCTCTACAGGAAACTGGGGCGCATC
GCGCCCGAACTCGCGTGGGTAACAAGGAGTATCGTCGCATCCAAGACATGAACCACACCCTCTCCAAACGACTCGTCGAATATGCCAGTCAGTTCGAGAA
TCCCGTTATCAAACTCGAAGACCTCGAAAACATCCGTGACGGTAGCGAGTGGAATGGCGTCCACTCGTGGCCGTTCTACGAACTCCAACAGTTCATCACG
TACAAAGCCGAGAAAGAAGGCGTTTGCGTCGAGAAAGTAGACCCCGAGAATACGAGTCAGGAGTGCAGTCAGTGCGATGAACTCGTGGGTCGTGACGGGA
GCAAGTTCGAGTGCCCGCACTGTGGGTATGTGCGGCACGCGGATTTGAACGCAGCTGAAAACATCAGTCAACGGGAGGGTGACCCATGCACGGCGTAACA
CTTCGGGTGACGCGAACCGTGCGGCCCAGCAGGATGAACAACCTGCCGACCACATCGTGGTCGCCTGCCACGTGCAGGTGGGAAGGCTCGTTTGACCGAG
CTATACGCGCTGGAAAGCACGCCCTTGGTCATAACTGGGCGGCGACCGTTGACGCTGGCCACGCGAAAGCGTATTTGAGCCGCCGAGGAAACGCGCAACC
CTAATATCCCCATCGGGGAATCCCACGGCTTCAGCCGTGGGAGGAGGTCAA
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
1053 bp350 aa1461198+No
ORF function : Accessory Gene
AG : TnpB

ORF sequence :

MVEVSVETTFRNPSRSRRQEWQRATHILRECKQWLVDGWEDDSLASSVTTGDIDNPLYSALQNQAIRNAKSDYADESIEYTGEQPIEVNNQNWEIHTTEN
DSVVIGFPCISDWWYTPIHVHESIEEPVQALLDGDAEKTKLSVWREGGDWHCSFTVEYDEQQDGEETPIGVDVGHNYILAATPDNASAESFLVSGKEHKF
VRRYYRSLRDSLQETGAHRARTRVGNKEYRRIQDMNHTLSKRLVEYASQFENPVIKLEDLENIRDGSEWNGVHSWPFYELQQFITYKAEKEGVCVEKVDP
ENTSQECSQCDELVGRDGSKFECPHCGYVRHADLNAAENISQREGDPCTA

 

Blast result :
Comments
ISNph24 is 74% (TnpB) aa similar to ISHbo7.
References
1] Pfeiffer F. (2013) Direct submission to ISfinder
2] Falb M., Pfeiffer F., Palm P., Rodewald K., Hickmann V., Tittor J., Oesterhelt D. (2005) Genome Res. 15: 1336-1343