ISHamu1

  • Family IS200/IS605
  • Group IS1341
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
AOLN01000006 ND Haloferax mucosum
Haloferax mucosum ATCC BAA-1512
DNA section
IS Length : 1475 bp

Ends


Left end : GGAAGTTGCAAGGCTAAAACCCCGCCCTTCAGGGCGGGGATACAGCCGACAACTCCTCCACTAGCCACGTTCGATGGCACGGTTGGATATTCCACGCCAA II struct. : Yes
Right end : TGAGGGTGTTTTCGCCCCACGAGAACAGGTTGCTAACCGCGAATCTTAATATCCCAACGCGGTTGGGAATCCTCGCCCTTCAGGGCGGGGAGGATGTCAA II struct. : Yes

Insertion site


Left flankLE cleavage siteRight flankRE cleavage site
GTCTGTGCGTAGTTATTCGCCGTCGCCCtcaa

DNA sequence

GGAAGTTGCAAGGCTAAAACCCCGCCCTTCAGGGCGGGGATACAGCCGACAACTCCTCCACTAGCCACGTTCGATGGCACGGTTGGATATTCCACGCCAA
CCGACACCATTAATAAAACGGCATTCATAACCAGTTATGACGCCAGAAAATGGGCCGAACCATCCGAACCTTCGAGGCCACCATTACGAACCAGCAACAG
GTTCGTGACGACCTTGACCAACTCGGATGGGCCGCCTCCAAACTCTGGAACGTCGGTCGCTACTACGCACAAGAACGGTGGGACGAAACGGGCGAGATTC
CCGACGACGGGGAACTCAAAACCGAACTCAAAGACCACGAACGCTACACGGACTTACATTCTCAATCCAGTCAGCGCGTTCTCGAAGAACTCGCTGAAGC
GTTCAACGGTTGGTTCAGTAAGCGTCGGAACGGCGACGACCGTGCCCGACCGCCCGGCTACCGCAAAAACGGAGACTCCCACCCGCGTTCAACCGTGTCG
TTCAAAGCGGCTGGCTTCAAGCACGACGCACAGTTCACCCGTGTCCGCCTCTCGAAAGGACGCAACCTCAAAGACCACCGGTCGGACTTCATCCTGTGTG
AGTACCAGACTCGCCCCGATGTTGACCTGACCGAGTGGGACATCCAACAGGTTCGCGCCGTCTACAAGCGCGACGAGTGGCGACTCCAGTTCGTCTGTCG
CACCACTATCGACCCGGAACCATCGGGCGATGAGGTGGCCGGTGTTGACCTCGGGATTTGCAACTTTGCCGCCGTCTCGTTCGGTGGTGAATCGGTGTTG
TACCCCGGTGGCGCACTCAAAGAGGACGAATACTACTTCACGAAACAGAAAGCCAAGTGCGACGATTCTTCGTCCCGAGAGGCCACTCGTCTTGACCGGA
AGCGTACTGGTCGCCGGACACACTTCTTACACGCACTCTCGAAAGCGATTGTTGAGGAGTGTACCGAACGAGGTGTCGGTACGCTTGTCGTTGGCGACCT
TGGCGGCATCCGAGAAGACGACGAGGACGGCAAACCTCGGAATTGGGGCGACCACGGCAATCTCGACTTGCACGGGTGGGCGTTCGACCGCTTCACGACG
CTTCTCGACTACAAGGCAGAAGCGGAAGGTATCGACGTGGCGTTGGTGTCGGAACGCGATACGTCAAAGTCGTGTTCGGCGTGCGGCCACACCGACGATA
GTCAGCGAGTTGAACGTGGCCTGTACGTGTGTGAAGCGTGCGATACGGTGGCGAACGCGGACGTGAATGGTGCGGAGAATATTCGACAAAAGGTACTCCC
GAGTCTCGCCACGGATGGCGGTGATAGGGATAACGGCTGGTTGGCACAGCCAGCGGTTCACCTGTTCGACCGTAGTGAGGGTGTTTTCGCCCCACGAGAA
CAGGTTGCTAACCGCGAATCTTAATATCCCAACGCGGTTGGGAATCCTCGCCCTTCAGGGCGGGGAGGATGTCAA
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
1275 bp424 aa1501424+No
ORF function : Accessory Gene
AG : TnpB

ORF sequence :

MGRTIRTFEATITNQQQVRDDLDQLGWAASKLWNVGRYYAQERWDETGEIPDDGELKTELKDHERYTDLHSQSSQRVLEELAEAFNGWFSKRRNGDDRAR
PPGYRKNGDSHPRSTVSFKAAGFKHDAQFTRVRLSKGRNLKDHRSDFILCEYQTRPDVDLTEWDIQQVRAVYKRDEWRLQFVCRTTIDPEPSGDEVAGVD
LGICNFAAVSFGGESVLYPGGALKEDEYYFTKQKAKCDDSSSREATRLDRKRTGRRTHFLHALSKAIVEECTERGVGTLVVGDLGGIREDDEDGKPRNWG
DHGNLDLHGWAFDRFTTLLDYKAEAEGIDVALVSERDTSKSCSACGHTDDSQRVERGLYVCEACDTVANADVNGAENIRQKVLPSLATDGGDRDNGWLAQ
PAVHLFDRSEGVFAPREQVANRES

 

Blast result :
Comments
ISHamu1 is 95% (TnpB) aa similar to ISHtu4.
References
1] Friedhelm Pfeiffer (2015à) Direct submission
2] Becker,E.A., Seitzer,P., Tritt,A., Larsen,D., Yao,A., Wu,D., Darling,A., Eisen,J.A. and Facciotti,M.T. (2012) Direct submission GenBank.