ISKpn31

  • Family ISAs1
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
GU595196 ND Klebsiella pneumoniae
Klebsiella pneumoniae 38544 plasmid p38544
Klebsiella pneumoniae plasmid pKpQIL
Klebsiella pneumoniae 32192 plasmid
DNA section
IS Length : 2328 bp

Ends


IR Length : 15/16

IRL : CAGGGCGAGAGCATGAGTTGATTTTTTGTTGTTTTTCATGATGAGATAGG
IRR : CAGGGCGAGAGCGTGAACATATTGTTAAAATTCACCCAATTTGTTGAGGC

Insertion site


Left flankDirect repeatRight flankDR Length
TTGACATCCACCCCGGCACAGTCCGCTGAA10

DNA sequence

CAGGGCGAGAGCATGAGTTGATTTTTTGTTGTTTTTCATGATGAGATAGGTGGCTCGGCCAATGCTGAACAAATTTGAGATCGCATTTTCAGCACACCCC
TGTACAAAATACGAGCAGAAAATGCAATTGCACCTTGGGATGACAGTGGTTAAATTTTTTTGGTTAACATGTCAGTAACTCATGCTCTCGCCCTGAGTTC
GCACCTTCCGCTACTTCGACAGTTGTGTAAAAAACCGTGCTCGTTGATAACCAGAAGGTGCGGTACTGAAAAACTATTGCTTGCAACAATTTGCTCAAAC
GTCACTATGGCTTTTCATGGTTTTTAACCCATTTTTGCATCTGGTCGATTTCTGGTTGCTGGGCTTTGATAATATCTTCTGCCAGCTTTCGCATTTCAGG
ATCCTTACCAAATTTAAGCTGCGTTTCAGACATTTGAATGGCACCTTGATGGTGGGCAATCATCCCTTTTGCAAAGGCAACATCTGGATCAGAGGACATC
ACTCCTGCCATCATGTCGTTATGCATACTTTTCATACCAGACATCAACTCTTGAGAAGCTGGAGACATGGAATGGTGATCGCCCATCGGCATGGTTTTGT
CAACCGCAAAAACAGCAACAGGAGACATTAATAGGGCGGTCAGTATCAATATTTTTTTGTTCATTTTACTACCTCTTAAAGAAAACTCGTTCGCTGAGAA
GTATTTACTTATCTCACTTCAAGTTACCTGACTATTGGACTTTGGCCTACAAGAAACTCGAATTATTCAGAGTGTAGATGGCGTTGTTTCCCAAATCAAC
CATCAAGCCACGACTGCCCCAGCCCCAAGTGACCGTTACACTCGGCGCTTTCTGACAGGCAGACCAAGGGGGTTCAGTTTGTTGATTGCACCAACATACG
CCATCACTTCCCCTACCTGACCGTTGTATGTTCGCAGACTGATTTTGCCCGTCATCAACTGCTTGAACCGATACATCGCCGTCTCTGCCTGCGAGCGACG
GTGATATCCCGATATCTTCTTCCAGTGCACCAGCCCTTCCTTGCGCATCACCAGCACCGCCTCATTTACGCCCCAGGGCAAGATCGCGAACGTTTTTTGA
CCAAGAAAAACAGGTTTGACCCTGCGATCCAGCTGTGATCTTATGCTCCTTTTTCAGGAGTGCCTTCATGTCTATTGATGCTGTTTTTGCTCAGTTTTTC
GATGATATTCCCGACCCTCGCCAGCATGCCAAGATTTATTATCCTTTCTACGATGTCCTGTTTCTCACCGTCTGCGCAGTGATAGGTGGCGCAGACGGGT
GGGAAGATATTGAAGATTTCGGTGAGGTTCATCTTCCTTGGTTTCAATCCAAGGGGCTGTTTAAAAATGGTATTCCTGTCCATGACACCATCGCCAGGAT
CATCTCCGGTATCAAACCAGAGCCGTTTCAGGCCGCGTTCGTACGTTGGACTCAAGCCATCAACCAGCACACCGAAGGCGCCTTGGTCGCTATCGATGGC
AAAACCCTGCGCAGTTCCTATAACCGTGAAGATCGAACGTCGACCATTCACATGGTAAGCGCCTATGCCGCCGCTAATAAACTGGTGCTCGGACAGATAA
AAACCGACGCCAAGTCCAATGAAATCACCGCCATTCCCGACTTGTTGGCGTTGCTCGACATCAAAGGCTGCCTGGTATCCATCGATGCGATGGGGTGCCA
AACCGAGATCGCCGAGACCATCCTCCAGCAAAGTGGCGATTACTTGTTAGCCGTCAAAGGCAACCAGCCCAAGTTGTTCGAGGCGGTCCGGCAAGCCCTG
GCCCCGCTGGCGGCTACCCCATTATGCGCAGCAACGATGACTCTTGATAAGGCTCATGGGCGCATCGATGGTCGTGAGTACCATATCATGGCGGCGGGTG
AGTTAGCCGCCCAGTTTCCTCATTGGGAACAGTTGCGCAGTATTGGCGTGGCCATCTCTTATCGCATTGAAAAAAGGGAAAAAATCACTATTGAACAGCG
TTATTACATCAGTTCCAAAGCACTGACGCGGGATGAGTTTGCACGATCGGTGCGTGGGCACTGGGCCATCGAAAATAGCCTGCACTGGGTGCTGGACGTC
ACGATGGGCGAGGACGATTGCCCAATTTATCGCGGTGATGCCGCAGAGATCCTTGCGTGCATCCGGCACATGGGGCTGAACATGCTCAGGGCTGAAACTT
CACGCAAAGCGAGCATCAGACGGAAGCAAAAGATTGCAGGAATGAGCAGTGAATACCTGGAAAAGGTACTCAATGCTGGCCTCAACAAATTGGGTGAATT
TTAACAATATGTTCACGCTCTCGCCCTG
Protein section
ORF number : 2

 

ORF 1
LengthBeginEndStrandFusion ORF
360 bp119 aa664305-No
ORF function : Passenger Gene
Annotation : Hypothetical proteinDescription :

ORF sequence :

MNKKILILTALLMSPVAVFAVDKTMPMGDHHSMSPASQELMSGMKSMHNDMMAGVMSSDPDVAFAKGMIAHHQGAIQMSETQLKFGKDPEMRKLAEDIIK
AQQPEIDQMQKWVKNHEKP

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
1137 bp378 aa11682304+No
ORF function : Transposase
Chemistry : Unknow

ORF sequence :

MSIDAVFAQFFDDIPDPRQHAKIYYPFYDVLFLTVCAVIGGADGWEDIEDFGEVHLPWFQSKGLFKNGIPVHDTIARIISGIKPEPFQAAFVRWTQAINQ
HTEGALVAIDGKTLRSSYNREDRTSTIHMVSAYAAANKLVLGQIKTDAKSNEITAIPDLLALLDIKGCLVSIDAMGCQTEIAETILQQSGDYLLAVKGNQ
PKLFEAVRQALAPLAATPLCAATMTLDKAHGRIDGREYHIMAAGELAAQFPHWEQLRSIGVAISYRIEKREKITIEQRYYISSKALTRDEFARSVRGHWA
IENSLHWVLDVTMGEDDCPIYRGDAAEILACIRHMGLNMLRAETSRKASIRRKQKIAGMSSEYLEKVLNAGLNKLGEF

 

Blast result :
Comments
The first ORF is a passenger gene (annotated as p019). The second ORF is the putative transposase, which is 97% aa similar to the transposase of ISAs12.
Tn4401 carrying the blaKPC carbapenemase gene is found inserted in ISKpn31 in several plasmids.
References
1] Partridge, S.R.(2014) J. Clin. Microbiol. 52, 4448-4449