ISKpn23

  • Family IS1380
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
KP689347 ND Klebsiella pneumoniae
Klebsiella pneumoniae strain A60136 plasmid p60136
DNA section
IS Length : 1671 bp

Ends


IR Length : 12/16

IRL : CCCACATTCCCCAAGTAAATCGCTGATTTTGCTGTCAGGATCTCAATATT
IRR : TTAACATACCCCAAGTGGCATGCCGTCTGACGTGAGTTTCGCATGAATCC

Insertion site


Left flankDirect repeatRight flankDR Length
TCTATTTACTACAGACACAG0

DNA sequence

CCCACATTCCCCAAGTAAATCGCTGATTTTGCTGTCAGGATCTCAATATTTCCGATATAAATCATGGTGTTGATGGCCGTGAGGGGCGCGTTTGATGGAT
CACCCAGAGGGTGCGGGTTCGAAGCGGGCAGATCGGGTCGAGTTCGACCGCCGGGTGCATGTGGAGTTCCGAGGTGCGCAGCTCAGTTCCGACGGCGGCC
TGCTGGTGATGCGCGAGCTGGATGACGCGCTCGGGCTGTCCGATCTGGCGGCAAAAGCATTGCGCGACACCCGGCGCGGCAAGAACACGGTCCACCGGCT
TGACGGGTTGTTCCGGCAATCGGTGTTCGGGCGACTGGCCGGATACGAGGATGTGAACGACGCCGACCGCCTGGCCCTCGATCCGGTGATGCGCCAGGTC
GTGGGTGGAAGGGCTGTCGATGCGCAAGCGGCCTCGGCCTCGCAGATGGGACGGTTCGAGACCGAGACACTGGCACAGCCCGAGAACCGTGCCGCGCTGG
CCGACCTGAATGGCCAATGGATCGACCGGTTCCATGACCGTAACGGGCTGAAGTACATCGTTCTGGACATGGACAGTTCGGTCAGCCCCACCCATGGCGA
TCAGGAGGGCGCGGCCTGGAACGGGCATTTCGACTGCACCTGCTATCACCCGAACTTTTTGTTCAACCAGTTCGGCATGCTGGAGCGCTGCGCCTTGCGC
CATGGCAACGTCCACAGCGCCGATGGCTGGCGGGACGTTCTCGACCCCGTCATCGCCCGCTACGCGGAGCGCGACCTTTGCGGCCGGTTCTTCCGGGCTG
ACGCCGCCTACGCGATCCCCGCGATCTATGAGCGGCTGGAAGAAGCCCGATTCTTCTACGCCATCCGGCTGCCTGCGAACAACGTCCTGCGCGAGAAGAT
CGCGCATCGGCTGACGCGCCCTGTCGGGCGACCTTCGCTGACCAAGGTCAAGCGCTTCTACGAAGACTTCGAGTATCAGGCGGCATCCTGGGACAAGGAA
CGCCGGGTGATCGCCAAGATCGAATGGCATCCGGGCGAACTGTTCCCGCGTGTCGGCTTCATCGTCACCAACCTGCCGATGGAGCCCGACTGGGTGGTGC
GGTTCTATAACCAGCGCGGCACCGCCGAGCAGCACATCAAGGAAGGTAAATATGCCTTCCACTGGACGCGGCTGTCATGCCGGAAGTTCCGCGACAATGA
GGTGCGACTGCAACTGCATGCGCTGGCCTACAACCTGGCCATCTTCTTGCGCTGCATCGAGTTGCCCGAGACGATGGCCGACTGGTCGTTGACCAGCCTG
CAACTGAAGCTGATCAAGATCGGGGCACGTGTCGTCCGCCATGCCCGCGCCATCACCTTCCAGCTGGCCGAGGTGGCCGTCACCGGTCCGATGGTCCGCG
CCATCCTCGCCGCCATCCGCCGATTGCGAGCGCCTCCGCTATGCGCATGACCGCCATCCACGCCCAAACTGAACGAAAACGGCAGGATGGGTCTGTCCGC
TGCGCTGAAAAACACCGCCACCAGGGCACAACACGGCGGCTTCGCGGGCCGATCTGCCCTGTCTCAGCGCCTTGCGCGCCCGACGACGCCGCTCGGGACG
GAAAATGATTGTCCTGCGGTCGGATTCATGCGAAACTCACGTCAGACGGCATGCCACTTGGGGTATGTTAA
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
1356 bp451 aa951450+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MDHPEGAGSKRADRVEFDRRVHVEFRGAQLSSDGGLLVMRELDDALGLSDLAAKALRDTRRGKNTVHRLDGLFRQSVFGRLAGYEDVNDADRLALDPVMR
QVVGGRAVDAQAASASQMGRFETETLAQPENRAALADLNGQWIDRFHDRNGLKYIVLDMDSSVSPTHGDQEGAAWNGHFDCTCYHPNFLFNQFGMLERCA
LRHGNVHSADGWRDVLDPVIARYAERDLCGRFFRADAAYAIPAIYERLEEARFFYAIRLPANNVLREKIAHRLTRPVGRPSLTKVKRFYEDFEYQAASWD
KERRVIAKIEWHPGELFPRVGFIVTNLPMEPDWVVRFYNQRGTAEQHIKEGKYAFHWTRLSCRKFRDNEVRLQLHALAYNLAIFLRCIELPETMADWSLT
SLQLKLIKIGARVVRHARAITFQLAEVAVTGPMVRAILAAIRRLRAPPLCA

 

Blast result :
Comments
ISKpn23 is 96% aa similar to ISApr9.
References
1] Nicoletti A.G., Marcondes M.F.M., Martins W.B., Almeida L.G.P., Nicolas M.F., Vasconcelos A.T.R., Oliveira V. and Gales A.C. (2015) Direct submission