ISNaoc5
- Family IS200/IS605
- Group IS1341
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| CP003929 | ND | Natronococcus occultus | Natronococcus occultus SP4 |
DNA section
IS Length : 1487 bp
Ends
Left end : CTGTAATCGAGGGCGCTAAGCCCCCGTTCTCAAGGAGCGAGCAATCCGAGGGCGTAGGGCGGGGATACAGCGCCCGCACGAGTATCAACCACCCTCAACC II struct. : Yes
Right end : CTGTGGATACGGTTTCCGTATCTGCAAGTCGCGTCGTCGAATCAGGAAGCCCCGCCCTCAAGGAGTCGCCGAAGGCGGCGAGTAGGGCGGGGTAGTTCAC II struct. : Yes
Insertion site
| Left flank | LE cleavage site | Right flank | RE cleavage site |
|---|---|---|---|
| TAGGCCCGCCCA | TTAG | GTACGTCGCCGA | tcac |
DNA sequence
CTGTAATCGAGGGCGCTAAGCCCCCGTTCTCAAGGAGCGAGCAATCCGAGGGCGTAGGGCGGGGATACAGCGCCCGCACGAGTATCAACCACCCTCAACC
TACATGTTTAACAAACCACAGAACGAAGATAGCGTACACGCGCATGGAGTACAGCCCACGTTTCAGGCTCTTGCCAACAACGAAACAGCGAGAGCTACTG
GGCTGGCAGCGGAACACCGTGCGCCAAGTCTACAACCACGCACTCTACCGTCTCGACCAGTTACCCAAAGACCCAGACCAAACAGTCCGCCAACGCGTCT
GGAAAGTCCGCGACGAACTCCCCGACTGGAAAATCAAGTGGGATGAGTGGGCCGACGTGTACTCCACCGTACTGCAAGCCGCCGTCGAACGCATCTACCA
CAGTATCACTGGCCTCGCCCAGAAACGCGATAACGGGCACAAAATCGGGAACTTGAACTGGAAGAAACCGAGAGAGTTCCAGAGTTTCACGTATCGCCAA
CGGGGCTTCGAACTCGACAAAAAGAGTGGTCCTGACGGCCGTGGACTCGTAACACTCAAGAAAGTACGTGGCGAGACGCTCGAAATCCCTATTCGACTCC
ACCGTAACATCGACCCGAACGACATCAAACACGTCACCGTCAAGCAAGAGGCAACGGGCGCGTGGTACGCCTCGTTCAGCGTCGAACGCGACGAACCAGA
GAAACCAGATCCCGAAGACATCGACCCAGAAGACACAGTAGGCCTCGACCTCGGCGTCCTCAACTTCATCCACGATTCGGACGGGCGGTCAATCAACCGC
CTCGACCTGTCCGACGACCGCGACCGGCTCGAACGCGAGCAACGCTCGCTCTCCCGGAAAGAGTACGAGTCGAACAACTGGTACAAACAACGCCAGCGAG
TTGCCGAGGTCCACATGGACATGCGGAACAAGAAACGCGATTACAAGCACAAGCTCGCCCATGCATACACGACCGAGTACGACGCCGTGTTCGTCGAGGA
TTTGGACGTGAAGTCGATGCTGGAAGGTGATGGTAACGCCCGAAACAAGCACGAGGTCGGCTGGCGCGAGTTCATTCGCATCCTCAAGCATCACGGGCGG
AAACACGGCTGTCACGTGGTGGAGGTCAAGCCTGAGGGGACGACGAAAGAGTGTGCCTCGTGTGGGGTGGAGACGGCGAAACCGGTGTGGGTACGCGAGC
ATTCGTGTCCAGCGTGTGGGTTCGAGTTGGACAGGGATTTCAATGCGTCGCTGAACGTTCTTTCTCGCGGTCTTGCCGAACTAGGAGTGGGTCACTCCGA
AGGAACGTCCTCAGAATCGCCTTGCGATTCTGCTGTGCGGACGAGAGCCCCGCTCTCGTCAACGCCTGTGGAGACTGCGACCGCTGTGGATACGGTTTCC
GTATCTGCAAGTCGCGTCGTCGAATCAGGAAGCCCCGCCCTCAAGGAGTCGCCGAAGGCGGCGAGTAGGGCGGGGTAGTTCAC
TACATGTTTAACAAACCACAGAACGAAGATAGCGTACACGCGCATGGAGTACAGCCCACGTTTCAGGCTCTTGCCAACAACGAAACAGCGAGAGCTACTG
GGCTGGCAGCGGAACACCGTGCGCCAAGTCTACAACCACGCACTCTACCGTCTCGACCAGTTACCCAAAGACCCAGACCAAACAGTCCGCCAACGCGTCT
GGAAAGTCCGCGACGAACTCCCCGACTGGAAAATCAAGTGGGATGAGTGGGCCGACGTGTACTCCACCGTACTGCAAGCCGCCGTCGAACGCATCTACCA
CAGTATCACTGGCCTCGCCCAGAAACGCGATAACGGGCACAAAATCGGGAACTTGAACTGGAAGAAACCGAGAGAGTTCCAGAGTTTCACGTATCGCCAA
CGGGGCTTCGAACTCGACAAAAAGAGTGGTCCTGACGGCCGTGGACTCGTAACACTCAAGAAAGTACGTGGCGAGACGCTCGAAATCCCTATTCGACTCC
ACCGTAACATCGACCCGAACGACATCAAACACGTCACCGTCAAGCAAGAGGCAACGGGCGCGTGGTACGCCTCGTTCAGCGTCGAACGCGACGAACCAGA
GAAACCAGATCCCGAAGACATCGACCCAGAAGACACAGTAGGCCTCGACCTCGGCGTCCTCAACTTCATCCACGATTCGGACGGGCGGTCAATCAACCGC
CTCGACCTGTCCGACGACCGCGACCGGCTCGAACGCGAGCAACGCTCGCTCTCCCGGAAAGAGTACGAGTCGAACAACTGGTACAAACAACGCCAGCGAG
TTGCCGAGGTCCACATGGACATGCGGAACAAGAAACGCGATTACAAGCACAAGCTCGCCCATGCATACACGACCGAGTACGACGCCGTGTTCGTCGAGGA
TTTGGACGTGAAGTCGATGCTGGAAGGTGATGGTAACGCCCGAAACAAGCACGAGGTCGGCTGGCGCGAGTTCATTCGCATCCTCAAGCATCACGGGCGG
AAACACGGCTGTCACGTGGTGGAGGTCAAGCCTGAGGGGACGACGAAAGAGTGTGCCTCGTGTGGGGTGGAGACGGCGAAACCGGTGTGGGTACGCGAGC
ATTCGTGTCCAGCGTGTGGGTTCGAGTTGGACAGGGATTTCAATGCGTCGCTGAACGTTCTTTCTCGCGGTCTTGCCGAACTAGGAGTGGGTCACTCCGA
AGGAACGTCCTCAGAATCGCCTTGCGATTCTGCTGTGCGGACGAGAGCCCCGCTCTCGTCAACGCCTGTGGAGACTGCGACCGCTGTGGATACGGTTTCC
GTATCTGCAAGTCGCGTCGTCGAATCAGGAAGCCCCGCCCTCAAGGAGTCGCCGAAGGCGGCGAGTAGGGCGGGGTAGTTCAC
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1335 bp | 444 aa | 144 | 1478 | + | No |
AG : TnpB
ORF sequence :
MEYSPRFRLLPTTKQRELLGWQRNTVRQVYNHALYRLDQLPKDPDQTVRQRVWKVRDELPDWKIKWDEWADVYSTVLQAAVERIYHSITGLAQKRDNGHK
IGNLNWKKPREFQSFTYRQRGFELDKKSGPDGRGLVTLKKVRGETLEIPIRLHRNIDPNDIKHVTVKQEATGAWYASFSVERDEPEKPDPEDIDPEDTVG
LDLGVLNFIHDSDGRSINRLDLSDDRDRLEREQRSLSRKEYESNNWYKQRQRVAEVHMDMRNKKRDYKHKLAHAYTTEYDAVFVEDLDVKSMLEGDGNAR
NKHEVGWREFIRILKHHGRKHGCHVVEVKPEGTTKECASCGVETAKPVWVREHSCPACGFELDRDFNASLNVLSRGLAELGVGHSEGTSSESPCDSAVRT
RAPLSSTPVETATAVDTVSVSASRVVESGSPALKESPKAASRAG
IGNLNWKKPREFQSFTYRQRGFELDKKSGPDGRGLVTLKKVRGETLEIPIRLHRNIDPNDIKHVTVKQEATGAWYASFSVERDEPEKPDPEDIDPEDTVG
LDLGVLNFIHDSDGRSINRLDLSDDRDRLEREQRSLSRKEYESNNWYKQRQRVAEVHMDMRNKKRDYKHKLAHAYTTEYDAVFVEDLDVKSMLEGDGNAR
NKHEVGWREFIRILKHHGRKHGCHVVEVKPEGTTKECASCGVETAKPVWVREHSCPACGFELDRDFNASLNVLSRGLAELGVGHSEGTSSESPCDSAVRT
RAPLSSTPVETATAVDTVSVSASRVVESGSPALKESPKAASRAG
Blast result :
Comments
ISNaoc5 is 90% (TnpB) aa similar to ISNph13.
References
1] Pfeiffer, F. (2015) Direct submission
2] Eisen,J., Huntemann,M., Wei,C.-L., Han,J., Detter,J.C., Han,C., Tapia,R., Chen,A., Kyrpides,N., Mavromatis,K., Markowitz,V., Szeto,E., Ivanova,N., Mikhailova,N., Ovchinnikova,G., Pagani,I., Pati,A., Goodwin,L., Nordberg,H.P., Cantor,M.N., Hua,S.X., Woyke,T., Eisen,J., Klenk,H.-P. and Klenk,H.-P. (2012) Direct submission GenBank.
2] Eisen,J., Huntemann,M., Wei,C.-L., Han,J., Detter,J.C., Han,C., Tapia,R., Chen,A., Kyrpides,N., Mavromatis,K., Markowitz,V., Szeto,E., Ivanova,N., Mikhailova,N., Ovchinnikova,G., Pagani,I., Pati,A., Goodwin,L., Nordberg,H.P., Cantor,M.N., Hua,S.X., Woyke,T., Eisen,J., Klenk,H.-P. and Klenk,H.-P. (2012) Direct submission GenBank.