ISNje1

  • Family IS200/IS605
  • Group IS1341
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
AOIA01000046 ND Natronococcus jeotgali
Natronococcus jeotgali DSM 18795
DNA section
IS Length : 1597 bp

Ends


Left end : TGAATTGAGGCGCTAAGGCCCCTTCCTCAACGAGCGACCGAAAGGAGCAAGTAGGGAGGGGATACAGCGCCGCACAGTTTTCATACACGTTCGACGCTCG II struct. : Yes
Right end : CCCTGTGGATACGTCCGTATCTGCAAAGCGCGTCGTTGAAACAGGAAGCCCCACCCTCAAGCGCGAGCCGTCAGGCGAGCGGTAGGGTGGGGTAGTTCAC II struct. : Yes

Insertion site


Left flankLE cleavage siteRight flankRE cleavage site
TGCCCGCTGTTCTTACatttaacaagtcac

DNA sequence

TGAATTGAGGCGCTAAGGCCCCTTCCTCAACGAGCGACCGAAAGGAGCAAGTAGGGAGGGGATACAGCGCCGCACAGTTTTCATACACGTTCGACGCTCG
GTCCACAGGCCCCCCGCCACCTTTATGTTAAAACGAGGTATAAACACAGGTGATGGATAGACATGAAATCGAAGGCCACGAAGTCATCGACGGGACGGCC
AAAGCCACCGGCAACGGCGCTCACGTCCTCGTCCCGAAACGGTGGCGTGGCGCAGACGTGAAAGTCATCCGAACCACCGAACCCGACGAGTAGCACCTCC
GATGAACTACAACTACAGGTATCGACTTCGACCGTCCGACGCTCTCGAACAGCAGTTAGCGTGGACTGTCGATACCTGTAGGCAGGTCTACAACCACTTC
CTCCACCGACTCAACCGCACCGACGATACCTCGGTATACAGTGAGCAGAAACTCCTGCCGAGCCTCAAGAAGAGGTGGAACGACCTGAAAAGCGTTCACT
CGAAAGTCCTACAGAAAGTCGTTCAGCGGTTGTACGACAACCTCTCGACGCTTCGCGGTCGCAAAGAGAATGGCTACCGCGTCGGGACGCTCAAGTGGAA
GGCTCCGGGCGAGTACCGCAGTTTCACCTACAGTCAATCCGGCTTCAAGCTCAAGAACACGGGCGGTCGGACTCGATTGTGGCTCTCGAAAATCGGTGAC
ATACCCCTCACCTTCCACCGCCACCTGCCCGACGACACCGAAATCAAGACCGTCACGGTCAAACACGAACCAACCGGCAAGTGGTACGCCATCCTTGGCG
TCGAAACCCCGGACGACCCGCCGGAGAAGCCGGAGAATCCCGAGAAGTGCGTCGGCATCGACGTGGGGATTCTCAAGTACGCTCACGACACCGACGGAAC
CGCCGTCGAATCGCTCAACTTGTCCGACGAGCGTGACCGCTTGGAACGCGCACAGCGCGACCTCTCGCGGAAACAGCACGGGTCTGCGAATTGGGAGAAA
CAGCGCCGTGTCGTGGCCGAGCGTCACGCCGACCTCAAGCGAAAGCGGCGGGACTTCCTCCACAAGTTGTCGAACCACTACGCGCGGGAGTACGACTTTG
TGGCAGTCGAAGACCTCGACGCGAAGGGGTTGGTCGAACTGCCGGGAAACTCACGCAACCGCGCAGGAGCGGCGTGGGGAACGTTCCTTCGGATGCTCGA
ATACAAGTGCGAACGCGAAGGAACACACTTCGTAGCGGTCAACCCGAGAGGGACGACCAAAGAATGCGCGTCCTGCGGTGTCTCGACGGACAAGCCGCTG
TGGGTCCGTGAACACTCCTGTCCCGCCTGCGGGTTCGAGGCGGACAGGGACGCGAACGCGGCGTGGAACATTCTTTCTCGCGGTATCAAGAAGCGGTTAG
GAGCGGGACGCTCCGAATCAATGTCCTCAGAATCGCCTTGCGATTCTGATGTGCGGACGAGAGCTCCGCTCTCGTCAACGCCTGTGGAGACTGCGCTCCC
TGTGGATACGTCCGTATCTGCAAAGCGCGTCGTTGAAACAGGAAGCCCCACCCTCAAGCGCGAGCCGTCAGGCGAGCGGTAGGGTGGGGTAGTTCAC
Protein section
ORF number : 2

 

ORF 1
LengthBeginEndStrandFusion ORF
141 bp46 aa153293+No
ORF function : Passenger Gene
Annotation : Hypothetical proteinDescription :

ORF sequence :

MDRHEIEGHEVIDGTAKATGNGAHVLVPKRWRGADVKVIRTTEPDE

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
1281 bp426 aa3021582+No
ORF function : Accessory Gene
AG : TnpB

ORF sequence :

MNYNYRYRLRPSDALEQQLAWTVDTCRQVYNHFLHRLNRTDDTSVYSEQKLLPSLKKRWNDLKSVHSKVLQKVVQRLYDNLSTLRGRKENGYRVGTLKWK
APGEYRSFTYSQSGFKLKNTGGRTRLWLSKIGDIPLTFHRHLPDDTEIKTVTVKHEPTGKWYAILGVETPDDPPEKPENPEKCVGIDVGILKYAHDTDGT
AVESLNLSDERDRLERAQRDLSRKQHGSANWEKQRRVVAERHADLKRKRRDFLHKLSNHYAREYDFVAVEDLDAKGLVELPGNSRNRAGAAWGTFLRMLE
YKCEREGTHFVAVNPRGTTKECASCGVSTDKPLWVREHSCPACGFEADRDANAAWNILSRGIKKRLGAGRSES

 

Blast result :
Comments
ISNje1 is 91% (TnpB) aa similar to ISHla22. The first ORF is passenger gene annotated as hypothetical protein.
References
1] Friedhelm Pfeiffer (2015) Direct submission.
2] Becker,E.A., Seitzer,P., Tritt,A., Larsen,D., Yao,A., Wu,D., Darling,A., Eisen,J.A. and Facciotti,M.T. (2012) Direct submission GenBank.