ISKox1

  • Family IS66
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
CP003684 ND Klebsiella oxytoca
Klebsiella pneumoniae
Klebsiella oxytoca E718 plasmid pKOX_R1
Enterobacter hormaechei strain E0083033-1 plasmid pEh1A
Enterobacter hormaechei subsp. oharae strain CCBH 10892 plasmid
DNA section
IS Length : 2526 bp

Ends


IR Length : 17/21

IRL : GTAAGCGTCAAGTCACTGCCGCCTGTGATTCCAGTCCCGGGATCGCTAG
IRR : GTAAGCGTTAAGTGAGCGCCGTATTGACGGTTATTTATCGGTAAGAACC

Insertion site


Left flankDirect repeatRight flankDR Length
TACCATCCCACAAAAAAGTTCGTGGTGG8
CTTAATGTTTATAAGGTCTGCTCTTGGT8

DNA sequence

GTAAGCGTCAAGTCACTGCCGCCTGTGATTCCAGTCCCGGGATCGCTAGCTTAGAGCTCCGTCTAATTTAGAAGGAGCTCTGTTTATGGAAAATGCTGCC
AACTGGCGAACTGAATCGCGTACCGTCTATTCCAGTGACTTCAAACTTCGGATGGTCGAACTGGCTTCACGACCAGATGCCAACGTCGCACAACTGGCGC
GGGAACATGGCGTTGATAATAATCTCATTTTTAAGTGGCTACGCCTCTGGCAGAGAGAGGGGCGAATCTCTCGTCGAATGCCTGCAACTATCGTGGGGCC
GGTGGTACCTCAATCTCTTCCGGCTTCTCCGACTCTGTTGTCCGTCGACGTTATCAACGACCCGTTGCCCGCCGCAGAGAATGACACTCTGTGTTCGTTC
TCCTCCGCTCACGCCAGCGCCACTTCCTGTCATGTTGAGTTCCGCCACGGCAAAATGACGCTGGAGAACCCGTCGTCAGAGTTGCTGACTGTGCTGATCC
GCGAACTGACCGGGAGGACACCATGATATCCCTCCCGTCAGGCACCCGCATCTGGCTGGTCGCCGGGGCCACGGATATGCGTAAATCCTTCAACGGGCTG
GGCGAACAGATACACCACGTTCTGGATGAGGATCCCTTCTCCGGCCATCTGTTTATCTTCCGGGGACGCCGTGGCGATACCGTGAAAATACTCTGGGCTG
ATGCTGATGGTCTGTGCCTGTTTATCAAACGCCTGGAAGAGGGACAGTTCGTCTGGCCTGCTGTACGCGACGGCAAAATCGCGATCACCCGCTCACAACT
TGCCATGCTCCTTGATAAGCTGGACTGGCGCCAGCCAAAAATATCCCGCCTTAACTCACTGACAATGTTGTAAAAAAATCATAACCGCATTATAAAAGCG
GTTATGAATCACGACTATCTCGCCCGTATCGCTGCGCTGGAAGACGCGCTTCGCCAGAAAGACAGTCAGCTCAGTCTCGTTGCAGAGACTGAGTCGTTCC
TGCGTTCTGCACTGGCCCGCGCAGAAGAGAAAATAGAGAACGAAGAGCGCGAGATAGAGCATCTGCGGGCACAGATAGAAAAACTGCGTCGAATGTTGTT
CGGTACCCGCTCCGAAAAACTTCGCCGACAGGTTGAAGAAGCCGAAGCCCTGCTGAAACAGCAGGAGCAGCAAAGCGATCGCTACAACGGACGGGAAGAC
GATCCGCAGGTACCTCGCCAGTTACGTCAGTCCCGCCATCGTCGCCCGTTACCGGCACACCTTCCCCGCGAGATACATCGGCTGGATCCTGCGGAAACCA
GCTGTCCGGAATGCGGCAGCGGTATGGCGTACCTCAGCGAAGTCAGCGTGGAGCAGCTGGAACTGGTCTCCAGCGCCCTGAAAGTGATCCGCACGGTCAG
GGTGAAAAAGGCCTGCACCCGATGTGACTGTATCGTTGAAGCACCTGCGCCATCACGTCCCATCGATCGGGGTATCGCCGGGCCGGGTCTGCTGGCCCGC
GTGTTAACGGCCAAATACTGCGAACACCTGCCGCTGTATCGCCAGTGCGAAATCTTTGCCCGTCAGGGCGTGGATCTGAGCCGGGCTCTGCTCTCCAACT
GGGTGGATGCCTGTTGCCGGTTAATGGCCCCGCTGGATGAGGCCCTTTACCACTACGTGATGGACTGCCACAAACTGCATACGGATGACACTCCGGTTCC
GGTGCTTGCACCGGGCAGAAAGAAGATGAAAACCGGGCGTATCTGGACGTATGTACGTGACGACAGAAGCGCAGGTTCATCAGATCCGCCAGCGGCATGG
TTCGCCTTCTCGCCAGACCGGCAGGGAAAACATCCTCAGCAACACCTTCGCCACTATCATGGTGTGCTGCAGGCGGATGCCTTCGCAGGCTACGATCGGT
TGTTCAGTCCGGAACGTGAAGGAGGGCCGCTGACAGAAGCCGCATGTTGGGCCCATGCCCGTCGAAAAATCCATGATGTCTATATAAGCACTCACACAGC
GACAGCAGAAGAAGCCCTGAAACGTATCGGTGAGCTGTACGCGATAGAAGAAGAAATACGCGGCCTCACGACAGAAGAGCGTCTGGCAGCCAGACAATCG
CAAAGTAAACCACTGCTGGCATCGCTGCATGAATGGCTGGTAGAAAAAAATGAGACGCTGTCGAAAAAGTCCCGTCTGGGCGAAGCGTTCGCTTATGTCC
TGAACCAGTGGGATGCGCTGTGCTACTACTGTGAAGATGGCCTGGCAGAGCCTGATAACAACGCTGCCGAACGAGCCCTTCGTGCCGTCTGTCTTGGGAA
AAAGAATTTTATCTTCTTCGGCAGCGACCACGGTGGTGAGCGCGGAGCCCTGCTGTACGGACTGATCGGGACGTGCAGGCTGAATGGTATCGATCCGGAA
GCCTACCTTCGCCATATCCTGAGCGTACTGCCGGAGTGGCCCAGCAACAAAGTGGCCGAACTGCTGCCATGGAACGTGGTTCTTACCGATAAATAACCGT
CAATACGGCGCTCACTTAACGCTTAC
Protein section
ORF number : 3

 

ORF 1
LengthBeginEndStrandFusion ORF
441 bp146 aa86526+No
ORF function : Accessory Gene
AG : IS66 TnpA

ORF sequence :

MENAANWRTESRTVYSSDFKLRMVELASRPDANVAQLAREHGVDNNLIFKWLRLWQREGRISRRMPATIVGPVVPQSLPASPTLLSVDVINDPLPAAEND
TLCSFSSAHASATSCHVEFRHGKMTLENPSSELLTVLIRELTGRTP

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
351 bp116 aa523873+No
ORF function : Accessory Gene
AG : IS66 TnpB

ORF sequence :

MISLPSGTRIWLVAGATDMRKSFNGLGEQIHHVLDEDPFSGHLFIFRGRRGDTVKILWADADGLCLFIKRLEEGQFVWPAVRDGKIAITRSQLAMLLDKL
DWRQPKISRLNSLTML

 

Blast result :
ORF 3
LengthBeginEndStrandFusion ORF
1593 bp530 aa9042496+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MNHDYLARIAALEDALRQKDSQLSLVAETESFLRSALARAEEKIENEEREIEHLRAQIEKLRRMLFGTRSEKLRRQVEEAEALLKQQEQQSDRYNGREDD
PQVPRQLRQSRHRRPLPAHLPREIHRLDPAETSCPECGSGMAYLSEVSVEQLELVSSALKVIRTVRVKKACTRCDCIVEAPAPSRPIDRGIAGPGLLARV
LTAKYCEHLPLYRQCEIFARQGVDLSRALLSNWVDACCRLMAPLDEALYHYVMDCHKLHTDDTPVPVLAPGRKKMKTGRIWTYVRDDRSAGSSDPPAAWF
AFSPDRQGKHPQQHLRHYHGVLQADAFAGYDRLFSPEREGGPLTEAACWAHARRKIHDVYISTHTATAEEALKRIGELYAIEEEIRGLTTEERLAARQSQ
SKPLLASLHEWLVEKNETLSKKSRLGEAFAYVLNQWDALCYYCEDGLAEPDNNAAERALRAVCLGKKNFIFFGSDHGGERGALLYGLIGTCRLNGIDPEA
YLRHILSVLPEWPSNKVAELLPWNVVLTDK

 

Blast result :
Comments
ISKox1 is 80%(orfA) and 97%(orfB) aa similar to ISEc47, the transposase (orfC) is 90% aa similar to ISEc23.
References
[1] Zhou D. (2016) Direct submission