ISKpn34
- Family IS3
- Group IS407
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| CP008932 | ND | Klebsiella pneumoniae | Klebsiella pneumoniae plasmid pNDM-MAR Citrobacter freundii plasmid pNDM-CIT Klebsiella pneumoniae strain PMK1 plasmid pPMK1-C Klebsiella pneumoniae strain PMK1 plasmid pPMK1-NDM Salmonella enterica strain F8025 plasmid pF8025 Escherichia coli strain Ecol_732 plasmid pEC732_IMP14 Citrobacter amalonaticus Y19 |
DNA section
IS Length : 1221 bp
Ends
IR Length : 33/45
IRL : TGACCTGCTCCCCGTTGATTAATACACCGTGATGTTAGTAATGTCTTCAT
IRR : TGACCTGCCCCCAGAGTTAGATACAACCTTCAGTTAGTAATGTCGGTTGG
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| CTAACAGGAA | CTCGCAATAC | 0 | |
| TTACATAGCT | CATT | ATTTTATAGT | 4 |
| GTTCTTGGTG | GAAG | TTATGCGACT | 4 |
| CCCTACGCGA | CATC(g/c)C | TTAATATAAA | 6 |
| TGTCGTTAAT | GCTC | ATTTATACTC | 4 |
| GCTGCCTGAG | TAGG | AGCTATAACA | 4 |
DNA sequence
TGACCTGCTCCCCGTTGATTAATACACCGTGATGTTAGTAATGTCTTCATAAGCCACATGAGGACATCCCCATGAAGAAGCGTTTTTCCGACGAACAGAT
CATATGTATTCTCCGCGAGGCCGAAGCCGGCGTTTCTGCCCGTGAGCTCTGCCGTAAGCACGCCATTTCCGACGCCACCTTTTACACATGGCGTAAGAAG
TATGGCGGTATGGAGGTGCCTGAGGTTAAGCGCCTGAAGTCGCTTGAGGAAGAGAACGCCAGACTCAAGAAGCTGCTTGCTGAAGCCATGCTGGATAAGG
AGGCGCTTCAGGTGGCTCTTGGGCGAAAGTACTGACGACAGACCAGAAGCGGGAAGCCGTGGAAGTCATGTGCGAGGCTAAGGGTCTGTCGCAACGTCGT
GCCTGCAGGCTGGCAGGTCTGTCCCTGTCAACCTGCCGATATTCGGCTCAGCGTCCGGCTGCTGACGCGCAGCTGTCTCTACGCATCACAGAGCTGGCAC
TTGAACGCCGCCGTTTTGGTTACCGGCGTATCTGGCAGCTTCTGCGACGTGAAGGTCTTTGCGTTAACCACAAGCGGGTTTACCGCATCTATCAACTTAA
TGGCCTGAGTGTAAAACGCAGACGACGTCGTAAAGGGCTGGCAACAGAACGTCTGCCGCTGCTCCGCCCGATGGCGCCCAATCTGACCTGGTCAATGGAT
TTCGTCATGGACGCACTGGCCACAGGTCGCAGGATCAAGTGCCTGACCTGCGTGGATGATTTCACAAAGGAATGCCTGACGGTCACTGTTGCCTTCGGGA
TTTCAGGCGTGCAGGTCACGCGTATTCTGGACAGCATTGCGCTGTTTCGCGGCTATCCGGCTATGATAAGAACCGATCAGGGCCCGGAGTTTACCTGCCG
CGCACTCGATCAGTGGGCTTTTGAGCATGGTGTGGAGCTGCGACTTATCCAGCCCGGCAAGCCAACGCAGAACGGATTTATTGAGAGTTTTAACGGACGC
TTTCGTGATGAATGCCTGAATGAGCACTGGTTCAGCGATATTGTTCACGCCAGGAAGATCATTAATGACTGGCGACTGGATTATAACGAGTGTCGACCAC
ATTCATCACTGAATTACCTGACGCCGGCTGAATTTGCAGCGGGCTGGCGAAACGGGAAATATGAAGAAAAACCAACCGACATTACTAACTGAAGGTTGTA
TCTAACTCTGGGGGCAGGTCA
CATATGTATTCTCCGCGAGGCCGAAGCCGGCGTTTCTGCCCGTGAGCTCTGCCGTAAGCACGCCATTTCCGACGCCACCTTTTACACATGGCGTAAGAAG
TATGGCGGTATGGAGGTGCCTGAGGTTAAGCGCCTGAAGTCGCTTGAGGAAGAGAACGCCAGACTCAAGAAGCTGCTTGCTGAAGCCATGCTGGATAAGG
AGGCGCTTCAGGTGGCTCTTGGGCGAAAGTACTGACGACAGACCAGAAGCGGGAAGCCGTGGAAGTCATGTGCGAGGCTAAGGGTCTGTCGCAACGTCGT
GCCTGCAGGCTGGCAGGTCTGTCCCTGTCAACCTGCCGATATTCGGCTCAGCGTCCGGCTGCTGACGCGCAGCTGTCTCTACGCATCACAGAGCTGGCAC
TTGAACGCCGCCGTTTTGGTTACCGGCGTATCTGGCAGCTTCTGCGACGTGAAGGTCTTTGCGTTAACCACAAGCGGGTTTACCGCATCTATCAACTTAA
TGGCCTGAGTGTAAAACGCAGACGACGTCGTAAAGGGCTGGCAACAGAACGTCTGCCGCTGCTCCGCCCGATGGCGCCCAATCTGACCTGGTCAATGGAT
TTCGTCATGGACGCACTGGCCACAGGTCGCAGGATCAAGTGCCTGACCTGCGTGGATGATTTCACAAAGGAATGCCTGACGGTCACTGTTGCCTTCGGGA
TTTCAGGCGTGCAGGTCACGCGTATTCTGGACAGCATTGCGCTGTTTCGCGGCTATCCGGCTATGATAAGAACCGATCAGGGCCCGGAGTTTACCTGCCG
CGCACTCGATCAGTGGGCTTTTGAGCATGGTGTGGAGCTGCGACTTATCCAGCCCGGCAAGCCAACGCAGAACGGATTTATTGAGAGTTTTAACGGACGC
TTTCGTGATGAATGCCTGAATGAGCACTGGTTCAGCGATATTGTTCACGCCAGGAAGATCATTAATGACTGGCGACTGGATTATAACGAGTGTCGACCAC
ATTCATCACTGAATTACCTGACGCCGGCTGAATTTGCAGCGGGCTGGCGAAACGGGAAATATGAAGAAAAACCAACCGACATTACTAACTGAAGGTTGTA
TCTAACTCTGGGGGCAGGTCA
Recoding section
- Recoding by frameshift
- Frame -1
- Type translational
- Experimentally demonstrated No
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 264 bp | 87 aa | 72 | 335 | + | No |
Description : First part of the transposase
ORF sequence :
MKKRFSDEQIICILREAEAGVSARELCRKHAISDATFYTWRKKYGGMEVPEVKRLKSLEEENARLKKLLAEAMLDKEALQVALGRKY
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 894 bp | 297 aa | 299 | 1192 | + | No |
Description : Second part of the transposase
ORF sequence :
GGASGGSWAKVLTTDQKREAVEVMCEAKGLSQRRACRLAGLSLSTCRYSAQRPAADAQLSLRITELALERRRFGYRRIWQLLRREGLCVNHKRVYRIYQL
NGLSVKRRRRRKGLATERLPLLRPMAPNLTWSMDFVMDALATGRRIKCLTCVDDFTKECLTVTVAFGISGVQVTRILDSIALFRGYPAMIRTDQGPEFTC
RALDQWAFEHGVELRLIQPGKPTQNGFIESFNGRFRDECLNEHWFSDIVHARKIINDWRLDYNECRPHSSLNYLTPAEFAAGWRNGKYEEKPTDITN
NGLSVKRRRRRKGLATERLPLLRPMAPNLTWSMDFVMDALATGRRIKCLTCVDDFTKECLTVTVAFGISGVQVTRILDSIALFRGYPAMIRTDQGPEFTC
RALDQWAFEHGVELRLIQPGKPTQNGFIESFNGRFRDECLNEHWFSDIVHARKIINDWRLDYNECRPHSSLNYLTPAEFAAGWRNGKYEEKPTDITN
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1121 bp | 373 aa | 72 | 1192 | + | Yes |
Chemistry : DDE
ORF sequence :
MKKRFSDEQIICILREAEAGVSARELCRKHAISDATFYTWRKKYGGMEVPEVKRLKSLEEENARLKKLLAEAMLDKEALQVALGRKVLTTDQKREAVEVM
CEAKGLSQRRACRLAGLSLSTCRYSAQRPAADAQLSLRITELALERRRFGYRRIWQLLRREGLCVNHKRVYRIYQLNGLSVKRRRRRKGLATERLPLLRP
MAPNLTWSMDFVMDALATGRRIKCLTCVDDFTKECLTVTVAFGISGVQVTRILDSIALFRGYPAMIRTDQGPEFTCRALDQWAFEHGVELRLIQPGKPTQ
NGFIESFNGRFRDECLNEHWFSDIVHARKIINDWRLDYNECRPHSSLNYLTPAEFAAGWRNGKYEEKPTDITN
CEAKGLSQRRACRLAGLSLSTCRYSAQRPAADAQLSLRITELALERRRFGYRRIWQLLRREGLCVNHKRVYRIYQLNGLSVKRRRRRKGLATERLPLLRP
MAPNLTWSMDFVMDALATGRRIKCLTCVDDFTKECLTVTVAFGISGVQVTRILDSIALFRGYPAMIRTDQGPEFTCRALDQWAFEHGVELRLIQPGKPTQ
NGFIESFNGRFRDECLNEHWFSDIVHARKIINDWRLDYNECRPHSSLNYLTPAEFAAGWRNGKYEEKPTDITN
Blast result :
Comments
ISKpn34 is 95% aa similar to ISSpr2.
The third ORF is a putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
The third ORF is a putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
References
[1] ISfinder (2016) Direct submission