ISAisp5

  • Family IS1595
  • Group ISSod11
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
AGTS01000077 ND Acidovorax sp.
Acidovorax sp. NO-1
DNA section
IS Length : 1088 bp

Ends


IR Length : 28/31

IRL : AAGGGGTATCTATTTAGCTCCAGTGGCGCCAAAATAGCTGGATGGAAACC
IRR : AAGGGGTATCTATTTAGCTCCAGCAGCCCCATTGATACTGTGATTTCATC

Insertion site


Left flankDirect repeatRight flankDR Length
ACGATACTGTACAAAACATCAGTATTTT8

DNA sequence

AAGGGGTATCTATTTAGCTCCAGTGGCGCCAAAATAGCTGGATGGAAACCCAGATGCCAACGCCAACTCCTGGACAGGACTACCCTCGCACCTGGAACGA
ATTTCTTGACTGGTTTGCCACCGAAGAGGCCTGCCAGGCGTTCCTGGAGAAGCTGCGCTGGCCCCAAGGCTTCGTCTGCCCGCGTTGCGGCAATGCAGGT
GATGTGTACCGCGCCAGCCGCACCCGCCTGATGTGCCGCTCATGCCAGTACCAAGGCACAGTGACCTCTGGAACCATCTTTGACAAGACGCGCACACCGC
TGCGTGTCTGGCTGGCGGCAGCTTGGTACCTGACCAATCAGAAGCAGGGCGTGAGCGCCCTGGGGCTGCAGCGCGTATTGGGTTTGGGTAGCTATCAGAC
TGCTTGGACGATGCTGCATCGGTTTCGGCGCGCCATGGTTCGCCCGGGCCGGGACAAGCTCAAGGGGCTTGTCGAGGTGGATGAAACGTACCTGTCCATT
ACGGATCGTAAAAATCCCGCCACTCCAGCAGGGCGCAAGAGCAGCACCACCAAAGTGTTGATGGTCATGGCGGTAGAGATCATGGAACCCAAGGGGTTCG
GGCGCATCCGGTTGCGCCGCATTGACCGAGACTCCGCCACCCACGTCATCCCCTTCGTGCAAGAGGTGGTCGAGCCCGGAGCCCAGGTACGCACGGACGG
TTCGGCGGCATACCGCGCACTGGGAGAACTGGGTTACACCCACCAGCGCACCGTCATGCTCGGTTCAGGTGTACCTGCCCATGTCTCCATGGCGGGAGTG
CACAGGGTCGCCTCACTGGTACAGCGCTGGGTGCTGGGAACGCACCACGGCTCTGTACAGCCCGAGCACCTGGATGCCTACCTCGATGAATTCGTGTTCC
GCTTCAACCGGCGCACATCCAGCTCGCGCAGGATGCTGTTTTACCGCCTACTGCAGCAAGCGGTGGTCACGCCGCCGGTGACATATGCGGATGTCGTGAA
TAAGAACACCGGGGATAGGCAATCGGATACTATTGCTGGATGAAATCACAGTATCAATGGGGCTGCTGGAGCTAAATAGATACCCCTT
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
990 bp329 aa541043+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MPTPTPGQDYPRTWNEFLDWFATEEACQAFLEKLRWPQGFVCPRCGNAGDVYRASRTRLMCRSCQYQGTVTSGTIFDKTRTPLRVWLAAAWYLTNQKQGV
SALGLQRVLGLGSYQTAWTMLHRFRRAMVRPGRDKLKGLVEVDETYLSITDRKNPATPAGRKSSTTKVLMVMAVEIMEPKGFGRIRLRRIDRDSATHVIP
FVQEVVEPGAQVRTDGSAAYRALGELGYTHQRTVMLGSGVPAHVSMAGVHRVASLVQRWVLGTHHGSVQPEHLDAYLDEFVFRFNRRTSSSRRMLFYRLL
QQAVVTPPVTYADVVNKNTGDRQSDTIAG

 

Blast result :
Comments
ISAisp5 is 98% aa similar to ISCsp2.
References
1] Thomoas Jové (2015) Direct submission
2] Huang,Y., Li,H., Rensing,C., Zhao,K., Johnstone,L. and Wang,G. (2012) J. Bacteriol. 194 (6), 1635-1636