ISNtsp2
- Family ISNCY
- Group ISDol1
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| CP002876 | ND | Nitrosomonas sp. | Nitrosomonas sp. Is79A3 |
DNA section
IS Length : 1886 bp
Ends
IR Length : 17/20
IRL : GTAGTGCAAGCCAGAAAACCATCTATCCCAATAAGAAACAACAGGTTGCT
IRR : GTAGTGTCAGCCCGAAAACCCCATTTTTTGACAGCGAGCTACCTAAATTT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| CTTGGTGAGTTGC | TTAA | GCAACTCACCAAG | 4 |
DNA sequence
GTAGTGCAAGCCAGAAAACCATCTATCCCAATAAGAAACAACAGGTTGCTAGTAATTAAGGTGATGAAGAATTCTGAGAAATTGAGCTAAACTGCTGTTA
TTAGGTAGTTCTGGTGCATTTTAAAGCAAAATAACCTCAAACTTCGATTGAAATCGGCGAGAATATCGTTGTCAGAAACAAACTTCGTTCGATTGGAGAT
CAGATGCGCGTAGTCCGAACTGCACAGATGGAATTAGGTGAAATTGACGTATCACAAATCAAATTCGATTTGAGATCACGAGATGACATACCGAAGATAC
TGCGCGGATTACAGCACCTGTACATGAATGAGGAATTGCGCCAGAGTGTTTTTGCATTACTGGAAAGTGAAATTGCTTCCGAGGTTAGTAAGCGCAATGG
CCGCCCTGGCATGACACTCTGGAGCATACTGGTTTGTGGCGTATTACGCCTGGACTTAAATGCTGATTATGACCGTCTTCACGAATTAGTTAACCAGCAT
AAAACTTTGCGCGAGATGCTGGGGCATAGTCTGTATGATGAAGACAAAAGCTATGCCTATCAAACTTTGGTGGATAATGTTGGCCTGCTCGCGCCGGAGC
TTTTGGACAAATTGAACCAAATCATTGTTGAAGGAGGGCATGCCCTTATAAAAAAGGGCGAAGGAGTCCTGCGTGGGCGGTGCGACTCCTTTGTTGTTGA
AACCGATGTGCATTTTCCAACCGACATCAACTTGTTGTGGGATGCCCTGCGCAAAGCGATTACGCTGACGGCATACTGGTGTGAAAGGCAGCAATTAAGC
GACTGGCGGCAATATAGCTACAACCTGCGCCAATTAAAACGACTCATGCGTTGTGCGCAGAACAAAAAGCGCAGCGTCGCTAAAGCACGACAAGACAAAA
TCGACGCACAAGTTACGCAAGCGCACCAGGCCTATATTGACCAAGCCAAAAATCACTTAGACAAAATTCAAGACACCTTAACCAAACTGACCGCGACTGC
GCCTACTGAGCTACTCCAAAAGATTGAAATTGAAGGCTACATCAAACACGCCGAGCGCCAAATCGACCAAATCGAACGTCGTGTCATTAAAGGTGAAATG
ATTCCTCACGCAGAAAAGGTATTCTCCATTTTTGAACCCCACACCGAATGGATCAGCAAAGGGAAGGCAGGCGTACCGGTTGAGTTGGGTGTCAAAGTAT
GCATCCTGGAAGACCAGCATCAATTCATCTTGCATCATCAGGTGATGGAAAAACAAACCGATGATCAGATTGCAGTATCCATGATCGCAGAAGCTAAAAA
ACGCTTTCCCAAACTCAATGCCTGTAGTTTCGACAAAGGCTTTCATTCACCCGCCAACCAAGCTGAATTGACCCAACATCTCGACCAGGTCACGCTCCCT
AGAAAAGGCAAACTAAGCAAAGAGCGTCAAGCGGTAGAACGAACCGAAGAATTTGTTAAAGCCCGTCGGGCACACTCAGCGGTAGAGTCAGCAATCAATG
CATTGGGGATACATGGTTTAGACAAATGTCCAGACCATGGCATCGATGGTTTTAAACGTTATGTTGCACTAGCCATTGTCACCAGGAACATCCGTCGAAT
GGGTGATATCCTGTGGCAGCAAGATGTGGAACGTGAGCGCAAAGCGAAAGAGCGATATTTAAAATATCGATCAGCAGCCTAAACAGCTGTCTATCAAACG
TAAAAACCGCCTGATTGTGAGCAATCAGCGGGTGGTGCTGGGTAAAAATCGAGTTTTTTGTGCTACGTCTCCCAAAAAAATGCAATCGGAAGCTTTTTTG
AATGCGTATCCTCATTGCATTTTTCAGGAGTGATTGAAATTTAGGTAGCTCGCTGTCAAAAAATGGGGTTTTCGGGCTGACACTAC
TTAGGTAGTTCTGGTGCATTTTAAAGCAAAATAACCTCAAACTTCGATTGAAATCGGCGAGAATATCGTTGTCAGAAACAAACTTCGTTCGATTGGAGAT
CAGATGCGCGTAGTCCGAACTGCACAGATGGAATTAGGTGAAATTGACGTATCACAAATCAAATTCGATTTGAGATCACGAGATGACATACCGAAGATAC
TGCGCGGATTACAGCACCTGTACATGAATGAGGAATTGCGCCAGAGTGTTTTTGCATTACTGGAAAGTGAAATTGCTTCCGAGGTTAGTAAGCGCAATGG
CCGCCCTGGCATGACACTCTGGAGCATACTGGTTTGTGGCGTATTACGCCTGGACTTAAATGCTGATTATGACCGTCTTCACGAATTAGTTAACCAGCAT
AAAACTTTGCGCGAGATGCTGGGGCATAGTCTGTATGATGAAGACAAAAGCTATGCCTATCAAACTTTGGTGGATAATGTTGGCCTGCTCGCGCCGGAGC
TTTTGGACAAATTGAACCAAATCATTGTTGAAGGAGGGCATGCCCTTATAAAAAAGGGCGAAGGAGTCCTGCGTGGGCGGTGCGACTCCTTTGTTGTTGA
AACCGATGTGCATTTTCCAACCGACATCAACTTGTTGTGGGATGCCCTGCGCAAAGCGATTACGCTGACGGCATACTGGTGTGAAAGGCAGCAATTAAGC
GACTGGCGGCAATATAGCTACAACCTGCGCCAATTAAAACGACTCATGCGTTGTGCGCAGAACAAAAAGCGCAGCGTCGCTAAAGCACGACAAGACAAAA
TCGACGCACAAGTTACGCAAGCGCACCAGGCCTATATTGACCAAGCCAAAAATCACTTAGACAAAATTCAAGACACCTTAACCAAACTGACCGCGACTGC
GCCTACTGAGCTACTCCAAAAGATTGAAATTGAAGGCTACATCAAACACGCCGAGCGCCAAATCGACCAAATCGAACGTCGTGTCATTAAAGGTGAAATG
ATTCCTCACGCAGAAAAGGTATTCTCCATTTTTGAACCCCACACCGAATGGATCAGCAAAGGGAAGGCAGGCGTACCGGTTGAGTTGGGTGTCAAAGTAT
GCATCCTGGAAGACCAGCATCAATTCATCTTGCATCATCAGGTGATGGAAAAACAAACCGATGATCAGATTGCAGTATCCATGATCGCAGAAGCTAAAAA
ACGCTTTCCCAAACTCAATGCCTGTAGTTTCGACAAAGGCTTTCATTCACCCGCCAACCAAGCTGAATTGACCCAACATCTCGACCAGGTCACGCTCCCT
AGAAAAGGCAAACTAAGCAAAGAGCGTCAAGCGGTAGAACGAACCGAAGAATTTGTTAAAGCCCGTCGGGCACACTCAGCGGTAGAGTCAGCAATCAATG
CATTGGGGATACATGGTTTAGACAAATGTCCAGACCATGGCATCGATGGTTTTAAACGTTATGTTGCACTAGCCATTGTCACCAGGAACATCCGTCGAAT
GGGTGATATCCTGTGGCAGCAAGATGTGGAACGTGAGCGCAAAGCGAAAGAGCGATATTTAAAATATCGATCAGCAGCCTAAACAGCTGTCTATCAAACG
TAAAAACCGCCTGATTGTGAGCAATCAGCGGGTGGTGCTGGGTAAAAATCGAGTTTTTTGTGCTACGTCTCCCAAAAAAATGCAATCGGAAGCTTTTTTG
AATGCGTATCCTCATTGCATTTTTCAGGAGTGATTGAAATTTAGGTAGCTCGCTGTCAAAAAATGGGGTTTTCGGGCTGACACTAC
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1479 bp | 492 aa | 204 | 1682 | + | No |
Chemistry : DDE
ORF sequence :
MRVVRTAQMELGEIDVSQIKFDLRSRDDIPKILRGLQHLYMNEELRQSVFALLESEIASEVSKRNGRPGMTLWSILVCGVLRLDLNADYDRLHELVNQHK
TLREMLGHSLYDEDKSYAYQTLVDNVGLLAPELLDKLNQIIVEGGHALIKKGEGVLRGRCDSFVVETDVHFPTDINLLWDALRKAITLTAYWCERQQLSD
WRQYSYNLRQLKRLMRCAQNKKRSVAKARQDKIDAQVTQAHQAYIDQAKNHLDKIQDTLTKLTATAPTELLQKIEIEGYIKHAERQIDQIERRVIKGEMI
PHAEKVFSIFEPHTEWISKGKAGVPVELGVKVCILEDQHQFILHHQVMEKQTDDQIAVSMIAEAKKRFPKLNACSFDKGFHSPANQAELTQHLDQVTLPR
KGKLSKERQAVERTEEFVKARRAHSAVESAINALGIHGLDKCPDHGIDGFKRYVALAIVTRNIRRMGDILWQQDVERERKAKERYLKYRSAA
TLREMLGHSLYDEDKSYAYQTLVDNVGLLAPELLDKLNQIIVEGGHALIKKGEGVLRGRCDSFVVETDVHFPTDINLLWDALRKAITLTAYWCERQQLSD
WRQYSYNLRQLKRLMRCAQNKKRSVAKARQDKIDAQVTQAHQAYIDQAKNHLDKIQDTLTKLTATAPTELLQKIEIEGYIKHAERQIDQIERRVIKGEMI
PHAEKVFSIFEPHTEWISKGKAGVPVELGVKVCILEDQHQFILHHQVMEKQTDDQIAVSMIAEAKKRFPKLNACSFDKGFHSPANQAELTQHLDQVTLPR
KGKLSKERQAVERTEEFVKARRAHSAVESAINALGIHGLDKCPDHGIDGFKRYVALAIVTRNIRRMGDILWQQDVERERKAKERYLKYRSAA
Blast result :
Comments
ISNtsp2 is 89% aa similar to ISNco1.
References
1] ISfinder annotation (2016)
2] Lucas,S., Han,J., Lapidus,A., Cheng,J.-F., Goodwin,L., Pitluck,S., Peters,L., Ovchinnikova,G., Lu,M., Detter,J.C., Han,C., Tapia,R., Land,M., Hauser,L., Kyrpides,N., Ivanova,N., Pagani,I., Bollmann,A., Norton,J., Suwa,Y., Klotz,M., Stein,L., Laanbroek,H., Arp,D. and Woyke,T. (2011) Direct submission GenBank.
2] Lucas,S., Han,J., Lapidus,A., Cheng,J.-F., Goodwin,L., Pitluck,S., Peters,L., Ovchinnikova,G., Lu,M., Detter,J.C., Han,C., Tapia,R., Land,M., Hauser,L., Kyrpides,N., Ivanova,N., Pagani,I., Bollmann,A., Norton,J., Suwa,Y., Klotz,M., Stein,L., Laanbroek,H., Arp,D. and Woyke,T. (2011) Direct submission GenBank.