IS903B
- Family IS5
- Group IS903
Isoform Synonym(s) IS903.B, ISKpn36
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| X02527 | Y | Escherichia coli | Enterobacterias phage HK022 Escherichia coli plasmid pRts1 |
DNA section
IS Length : 1057 bp
Ends
IR Length : 18
IRL : GGCTTTGTTGAATAAATCGAACTTTTGCTGAGTTGAAGGATCAGATCACG
IRR : GGCTTTGTTGAATAAATCAGATTTCGGGTAAGTCTCCCCCGTAGCGGGTT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| GAGATTTTGA | GACACAACG | TGTTCCCCGC | 9 |
| NNNNNNNGAT | CGTTGGGAA | CCGNNNNNNN | 9 |
DNA sequence
GGCTTTGTTGAATAAATCGAACTTTTGCTGAGTTGAAGGATCAGATCACGCATCTTCCCGACAACGCAGACCGTTCCGTGGCAAAGCAAAAGTTCAAAAT
CACCAACTGGCCCACCTACAATAAAGCCCTCATCAACCGTGGCTCCATAACTTTCTGGCTGGATGATGAAGCTATTCAGGCCTGGTATGAGTCAGCAACA
CCTTCTTCACGAGGCAGACCTCAGCGCTATTCTGACCTTGCCATCACGACTGTGCTGGTCATTAAACGCGTATTCAGGCTGACCCTGCGCGCTGCCCAGG
GCTTTATTGATTCCATTTTTTCTCTGATGAACGTTCCGCTACGCTGCCCGGATTACAGCTGTGTCAGCAGGCGGGCAAAGTCGGTTAATGTCAGTTTCAA
AACGCCCACCCGGGGTGAAATCGCACACCTGGTAATTGATTCCACCGGGCTGAAGGTCTTCGGTGAAGGCGAGTGGAAAGTCAAAAAGCATGGCCAGGAA
CGCCGTCGTATATGGCGAAAGTTGCATCTGGCCGTTGACAGCAACACACATGAAATCATCTGTGCAGACCTGTCGCTGAACAATGTGACGGACTCAGAAG
CCTTCCCGGGTCTTATCCGGCAGACTCACAGAAAAATCAGGGCAGCAGCGGCAGACGGCGCTTACGACACCCGGCTCTGTCACGATGAACTGCGGCGTAA
GAAAATCAGCGCGCTTATCCCTCCCCGAAAAGGTGCAGGTTACTGGCCCGGTGAATATGCAGACCGTAACCGTGCAGTGGCTAATCAGCGAATGACCGGG
AGTAATGCGCGGTGGAAATGGACAACAGATTACAACCGTCGCTCGATAGCGGAAACGGCGATGTACCGGGTAAAACAGCTGTTCGGGGGTTCACTGACGC
TGCGTGACTACGATGGTCAGGTTGCGGAGGCTATGGCCCTGGTACGAGCGCTGAACAAAATGACGAAAGCAGGTATGCCTGAAAGCGTGCGTATTGCCTG
AAAACACAACCCGCTACGGGGGAGACTTACCCGAAATCTGATTTATTCAACAAAGCC
CACCAACTGGCCCACCTACAATAAAGCCCTCATCAACCGTGGCTCCATAACTTTCTGGCTGGATGATGAAGCTATTCAGGCCTGGTATGAGTCAGCAACA
CCTTCTTCACGAGGCAGACCTCAGCGCTATTCTGACCTTGCCATCACGACTGTGCTGGTCATTAAACGCGTATTCAGGCTGACCCTGCGCGCTGCCCAGG
GCTTTATTGATTCCATTTTTTCTCTGATGAACGTTCCGCTACGCTGCCCGGATTACAGCTGTGTCAGCAGGCGGGCAAAGTCGGTTAATGTCAGTTTCAA
AACGCCCACCCGGGGTGAAATCGCACACCTGGTAATTGATTCCACCGGGCTGAAGGTCTTCGGTGAAGGCGAGTGGAAAGTCAAAAAGCATGGCCAGGAA
CGCCGTCGTATATGGCGAAAGTTGCATCTGGCCGTTGACAGCAACACACATGAAATCATCTGTGCAGACCTGTCGCTGAACAATGTGACGGACTCAGAAG
CCTTCCCGGGTCTTATCCGGCAGACTCACAGAAAAATCAGGGCAGCAGCGGCAGACGGCGCTTACGACACCCGGCTCTGTCACGATGAACTGCGGCGTAA
GAAAATCAGCGCGCTTATCCCTCCCCGAAAAGGTGCAGGTTACTGGCCCGGTGAATATGCAGACCGTAACCGTGCAGTGGCTAATCAGCGAATGACCGGG
AGTAATGCGCGGTGGAAATGGACAACAGATTACAACCGTCGCTCGATAGCGGAAACGGCGATGTACCGGGTAAAACAGCTGTTCGGGGGTTCACTGACGC
TGCGTGACTACGATGGTCAGGTTGCGGAGGCTATGGCCCTGGTACGAGCGCTGAACAAAATGACGAAAGCAGGTATGCCTGAAAGCGTGCGTATTGCCTG
AAAACACAACCCGCTACGGGGGAGACTTACCCGAAATCTGATTTATTCAACAAAGCC
Protein section
ORF number : 2
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 924 bp | 307 aa | 78 | 1001 | + | No |
Chemistry : DDE
ORF sequence :
MAKQKFKITNWPTYNKALINRGSITFWLDDEAIQAWYESATPSSRGRPQRYSDLAITTVLVIKRVFRLTLRAAQGFIDSIFSLMNVPLRCPDYSCVSRRA
KSVNVSFKTPTRGEIAHLVIDSTGLKVFGEGEWKVKKHGQERRRIWRKLHLAVDSNTHEIICADLSLNNVTDSEAFPGLIRQTHRKIRAAAADGAYDTRL
CHDELRRKKISALIPPRKGAGYWPGEYADRNRAVANQRMTGSNARWKWTTDYNRRSIAETAMYRVKQLFGGSLTLRDYDGQVAEAMALVRALNKMTKAGM
PESVRIA
KSVNVSFKTPTRGEIAHLVIDSTGLKVFGEGEWKVKKHGQERRRIWRKLHLAVDSNTHEIICADLSLNNVTDSEAFPGLIRQTHRKIRAAAADGAYDTRL
CHDELRRKKISALIPPRKGAGYWPGEYADRNRAVANQRMTGSNARWKWTTDYNRRSIAETAMYRVKQLFGGSLTLRDYDGQVAEAMALVRALNKMTKAGM
PESVRIA
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 213 bp | 70 aa | 672 | 460 | - | No |
Annotation : Hypothetical proteinDescription :
ORF sequence :
MCVAVNGQMQLSPYTTAFLAMLFDFPLAFTEDLQPGGINYQVCDFTPGGRFETDINRLCPPADTAVIRAA
Blast result :
Comments
IS903B differs from IS903 and IS102 by 34 and 61 nt, respectively.
Additionnal Accession number :
NC_002166 : This sequence is 99% aa similar to the sequence of IS903B ORF1.
KT362307 : this sequence is also 99% aa similar to IS903B.
Found in Tn2680.
Additionnal Accession number :
NC_002166 : This sequence is 99% aa similar to the sequence of IS903B ORF1.
KT362307 : this sequence is also 99% aa similar to IS903B.
Found in Tn2680.
References
1] Mollet, B., Iida, S., and Arber, W. (1985) An active variant of the prokaryotic transposable element IS903 carries an amber stop codon in the middle of an open reading frame. Mol. Gen. Genet. 199, 534-536.
2] Mollet, B., Clerget, M., Meyer, J., and Iida, S. (1985). J. Bacteriol. 163, 55-60.
3] Juhala,R., Ford,M.E., Duda,R.L., Youlton,A., Hatfull,G.F. and Hendrix,R.W. (1998) Direct submission GenBank.
4] Li,X., Yu,Y. and Fu,Y. (2015) Direct GenBank submission.
2] Mollet, B., Clerget, M., Meyer, J., and Iida, S. (1985). J. Bacteriol. 163, 55-60.
3] Juhala,R., Ford,M.E., Duda,R.L., Youlton,A., Hatfull,G.F. and Hendrix,R.W. (1998) Direct submission GenBank.
4] Li,X., Yu,Y. and Fu,Y. (2015) Direct GenBank submission.