ISKra7
- Family ISNCY
- Group ISDol1
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| ADVG01000001 | ND | Ktedonobacter racemifer | Ktedonobacter racemifer DSM 44963 |
DNA section
IS Length : 1586 bp
Ends
IR Length : 20
IRL : GTTTGCGGTGCGAAATGGAGCCACTGAAGCGGCTTGAGAGCAGAAAGAGC
IRR : GTTTGCGGTGCGAAATGGAGAGAAGCGCATGAGAAGGGCGTTGAGAGCCA
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| GGGAATTTTCTCC | TAGTACTA | GTGCTGCGTTCCTCA | 8 |
| CGCAAGGGATAC | TAGTACTA | GTCGTATGTGGC | 8 |
| TAAGTGGTGCTG | TAGTACTA | GTACATCGGCAG | 8 |
| GGCGACGACACT | TAGTACTA | CAGTCCCACTTA | 8 |
DNA sequence
GTTTGCGGTGCGAAATGGAGCCACTGAAGCGGCTTGAGAGCAGAAAGAGCGAAGGAAGAGCGTGGTAGAATGAACAAGAGGGGCTTATCAGGCAGAGAAA
GGAAGGGCCATGCTCATCGATTGCTACCAACCAGAAGATGTGTTTGCCCGCGTGCCCGAGGTGGCAGCCCAAACCGATCCTGTCCTCAAGCAGTTGGATG
GGTTACTCGACGATGATGACCTCTATCAGCACGTCCGTGGAGATCTGGGGAAGCGCTATCGCTGGACGCTGGTGCATGGGCGGCATTCGACGCCGGTGGA
AGTGATCTTACGCATGCTCATCTGCAAACACTTGTATCAGTGGAGCTTTCAAGAAACGGAGGAACGCGTCAAGGACAGCCTGGTGCTGCGCTGGTTTTGC
CGCGTCTATTTTGAGCGAGTCCCTGATGATACCACGCTCATTCGCTGGGCCCACACCATCCGCCCAGAGACGGTGCATACCCTCAACGACCGGGTGGTGC
ATCTGGCGAGGCAGGCCAAGGTCACCAGCGGACGCAAACTGCGCCTCGATGCCACCTGTGTGCAGACCAACATCCATCATCCCACTGATAGTGGCTTGCT
GGTGGACGGCGTGAGGGTGCTTTCCCGCTTCGTGCAACGGGCCAAACCGCTGGTGGAGGGCACGCTCACGTCGGTAAAGCAAGCCTGCCGCAGCCGAGTG
CGCTCAGCGCGTCGTGCTGCGCAGTCGTTGCATCGGCTGTTGCGGCGCAAAGGGGAGCAGAAAGAGGAGCAACAACGAGCGCTCTACCACAAGTTGATCA
CGACCACCCAGCAAATGGTCAAGCAGGCCAAGCAGGTTGCCGCGAACCTGAGCTGCCAGGCGGAGGCACCAGCCCAGCGACTGCTTCTTTCAGCTCAGCA
GGTGCTCCCGCTTATCGAACGGGTCATCACGCAAGCCCACGTCCGAGTGCTGGAAGGCAAGAAGGTGGCTGCCAGCGAGAAAGTCCTCAGCCTCTTCGAA
CCGCATACCCGTGCTATCCCTCGCCACAAAGGTGGGGCCCTGGTGGAGTTCGGTCGGCACGTGGTGCTCGACGAGGTAGAGGGGGGCATCGTCACGCGCT
TTGAGATCTGCGAGGCTCCTCATGAGCATGGACAGGCTGATGAAGCACTTGCCCATCATCAGCCCCTCTTTGCGCACCCTCCGCGCTGGTTGGTCGGCGA
TCGCGGGGTTCACGCTGCAGGGACGCGTGAGCGAGCGCAAGCCGCAGGGGTCAAGGTGGTGGCGATCCCTGCGGAGGGCAAGCGCTCGCAGGAGCAACGA
GCCCAAGAACACACACGAGCCTTCAAACGAGCCTACCGCTGGCGTGCGGGGATCGAGGGGCGCATTCACAGCCTGCGCCGCGATTATGGGTTGCGCAGAT
GTGCCTATCACGGGGAAGACGGGATGCAACGTTGGATCGGTCTGGGCATCATCTCCAGTAACCTGCGCCATATTGCCCAGGCCACGGTCGCCTAGGGCTC
ATCCAGAGAGAACCTCATCCATGATACCGAGAGCAATGGCTCTCAACGCCCTTCTCATGCGCTTCTCTCCATTTCGCACCGCAAAC
GGAAGGGCCATGCTCATCGATTGCTACCAACCAGAAGATGTGTTTGCCCGCGTGCCCGAGGTGGCAGCCCAAACCGATCCTGTCCTCAAGCAGTTGGATG
GGTTACTCGACGATGATGACCTCTATCAGCACGTCCGTGGAGATCTGGGGAAGCGCTATCGCTGGACGCTGGTGCATGGGCGGCATTCGACGCCGGTGGA
AGTGATCTTACGCATGCTCATCTGCAAACACTTGTATCAGTGGAGCTTTCAAGAAACGGAGGAACGCGTCAAGGACAGCCTGGTGCTGCGCTGGTTTTGC
CGCGTCTATTTTGAGCGAGTCCCTGATGATACCACGCTCATTCGCTGGGCCCACACCATCCGCCCAGAGACGGTGCATACCCTCAACGACCGGGTGGTGC
ATCTGGCGAGGCAGGCCAAGGTCACCAGCGGACGCAAACTGCGCCTCGATGCCACCTGTGTGCAGACCAACATCCATCATCCCACTGATAGTGGCTTGCT
GGTGGACGGCGTGAGGGTGCTTTCCCGCTTCGTGCAACGGGCCAAACCGCTGGTGGAGGGCACGCTCACGTCGGTAAAGCAAGCCTGCCGCAGCCGAGTG
CGCTCAGCGCGTCGTGCTGCGCAGTCGTTGCATCGGCTGTTGCGGCGCAAAGGGGAGCAGAAAGAGGAGCAACAACGAGCGCTCTACCACAAGTTGATCA
CGACCACCCAGCAAATGGTCAAGCAGGCCAAGCAGGTTGCCGCGAACCTGAGCTGCCAGGCGGAGGCACCAGCCCAGCGACTGCTTCTTTCAGCTCAGCA
GGTGCTCCCGCTTATCGAACGGGTCATCACGCAAGCCCACGTCCGAGTGCTGGAAGGCAAGAAGGTGGCTGCCAGCGAGAAAGTCCTCAGCCTCTTCGAA
CCGCATACCCGTGCTATCCCTCGCCACAAAGGTGGGGCCCTGGTGGAGTTCGGTCGGCACGTGGTGCTCGACGAGGTAGAGGGGGGCATCGTCACGCGCT
TTGAGATCTGCGAGGCTCCTCATGAGCATGGACAGGCTGATGAAGCACTTGCCCATCATCAGCCCCTCTTTGCGCACCCTCCGCGCTGGTTGGTCGGCGA
TCGCGGGGTTCACGCTGCAGGGACGCGTGAGCGAGCGCAAGCCGCAGGGGTCAAGGTGGTGGCGATCCCTGCGGAGGGCAAGCGCTCGCAGGAGCAACGA
GCCCAAGAACACACACGAGCCTTCAAACGAGCCTACCGCTGGCGTGCGGGGATCGAGGGGCGCATTCACAGCCTGCGCCGCGATTATGGGTTGCGCAGAT
GTGCCTATCACGGGGAAGACGGGATGCAACGTTGGATCGGTCTGGGCATCATCTCCAGTAACCTGCGCCATATTGCCCAGGCCACGGTCGCCTAGGGCTC
ATCCAGAGAGAACCTCATCCATGATACCGAGAGCAATGGCTCTCAACGCCCTTCTCATGCGCTTCTCTCCATTTCGCACCGCAAAC
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1386 bp | 461 aa | 110 | 1495 | + | No |
Chemistry : DDE
ORF sequence :
MLIDCYQPEDVFARVPEVAAQTDPVLKQLDGLLDDDDLYQHVRGDLGKRYRWTLVHGRHSTPVEVILRMLICKHLYQWSFQETEERVKDSLVLRWFCRVY
FERVPDDTTLIRWAHTIRPETVHTLNDRVVHLARQAKVTSGRKLRLDATCVQTNIHHPTDSGLLVDGVRVLSRFVQRAKPLVEGTLTSVKQACRSRVRSA
RRAAQSLHRLLRRKGEQKEEQQRALYHKLITTTQQMVKQAKQVAANLSCQAEAPAQRLLLSAQQVLPLIERVITQAHVRVLEGKKVAASEKVLSLFEPHT
RAIPRHKGGALVEFGRHVVLDEVEGGIVTRFEICEAPHEHGQADEALAHHQPLFAHPPRWLVGDRGVHAAGTRERAQAAGVKVVAIPAEGKRSQEQRAQE
HTRAFKRAYRWRAGIEGRIHSLRRDYGLRRCAYHGEDGMQRWIGLGIISSNLRHIAQATVA
FERVPDDTTLIRWAHTIRPETVHTLNDRVVHLARQAKVTSGRKLRLDATCVQTNIHHPTDSGLLVDGVRVLSRFVQRAKPLVEGTLTSVKQACRSRVRSA
RRAAQSLHRLLRRKGEQKEEQQRALYHKLITTTQQMVKQAKQVAANLSCQAEAPAQRLLLSAQQVLPLIERVITQAHVRVLEGKKVAASEKVLSLFEPHT
RAIPRHKGGALVEFGRHVVLDEVEGGIVTRFEICEAPHEHGQADEALAHHQPLFAHPPRWLVGDRGVHAAGTRERAQAAGVKVVAIPAEGKRSQEQRAQE
HTRAFKRAYRWRAGIEGRIHSLRRDYGLRRCAYHGEDGMQRWIGLGIISSNLRHIAQATVA
Blast result :
Comments
ISKra7 is 61% aa similar to ISTca1.
References
1] ISfinder annotation (2016)
2] Chang,Y.J., Land,M., Hauser,L., Chertkov,O., Del Rio,T.G., Nolan,M., Copeland,A., Tice,H., Cheng,J.F., Lucas,S., Han,C., Goodwin,L., Pitluck,S., Ivanova,N., Ovchinikova,G., Pati,A., Chen,A., Palaniappan,K., Mavromatis,K., Liolios,K., Brettin,T., Fiebig,A., Rohde,M., Abt,B., Goker,M., Detter,J.C., Woyke,T., Bristow,J., Eisen,J.A., Markowitz,V., Hugenholtz,P., Kyrpides,N.C., Klenk,H.P. and Lapidus,A. (2011) Stand Genomic Sci 5 (1), 97-111.
2] Chang,Y.J., Land,M., Hauser,L., Chertkov,O., Del Rio,T.G., Nolan,M., Copeland,A., Tice,H., Cheng,J.F., Lucas,S., Han,C., Goodwin,L., Pitluck,S., Ivanova,N., Ovchinikova,G., Pati,A., Chen,A., Palaniappan,K., Mavromatis,K., Liolios,K., Brettin,T., Fiebig,A., Rohde,M., Abt,B., Goker,M., Detter,J.C., Woyke,T., Bristow,J., Eisen,J.A., Markowitz,V., Hugenholtz,P., Kyrpides,N.C., Klenk,H.P. and Lapidus,A. (2011) Stand Genomic Sci 5 (1), 97-111.