ISVa12
- Family IS66
- Group
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| ND | Vibrio anguillarum | Vibrio anguillarum serovar O2 (VIB12) |
DNA section
IS Length : 2378 bp
Ends
IR Length : 14/19
IRL : GTAAGCGCGTCATAAACACCGCATTCTAATCAACCTCAGCACGCTATACG
IRR : GTATGCGCCCCATAATCCCACGGGGCGTTTTAATTTCAGTGTGAGACGTT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| GCAACGACCA | AAATAAAG | CTGTCTTCCT | 8 |
| TTTCAAAACC | AAAGAACG | AGCTTTTTTG | 8 |
DNA sequence
GTAAGCGCGTCATAAACACCGCATTCTAATCAACCTCAGCACGCTATACGATCAAACCTCCAACCTCAAGGAGATTTGAAATGGTAAAACGACGCACCAA
CCAAGAATGGCAAACCTTGTTCGAGCAATACCAATCCAGTCATCTATCACAGCGGGTCTTCTGTGAGCGTAATGGGTTAAGTCTTTCTACTTTTTATGCC
AAACGCCAGCAGTTACAAACTACGGAGTCAGCCAATATCGGTGGCTTTGTTAGAGCAGAAGTTATCGAAAAAACGACTCGCTACCAAGTGGCTCAAGCAC
CCGTCGCCAACATGACGCTATCCATTAATGATGTTGAACTCAGCATTCCCCAGGGAACACCTGTGGCCTACTTAGCCGAGTTAATCGGAGTGCTGTCATG
AAGAGAATGCTTAGTGCACCCAATATTTACTTGTATCGAGAGTTCGTGGACTTTAGAAAATCCATCAATGGATTAGCAGCGATTATCGAGTCCGAAACCG
ATTTGCCGCTAGGTACAGGTGCGCTATTCCTGTTTACCAATAAACAACGCGACAAAGTCAAAGTGTTGTACTGGGATAAAACGGGCTTTGCCCTGTGGTA
CAAACGGTTGGAAAAAGCCAAATTCAAATGGCCCACTCAAGAGAAAAAGCAGGTCTACACCCTGACTCAATTTGACTTAGATAGACTGTTATCTGGCTTC
ACGATAATCGGCCATAAACCGATAAAAATAGACAACTTTACAATGAGTTGAACCCGATAAAAGTGAGTTATACCAAGCCTTGACGTGGTGTTTTTAATAT
AATCAATGCCATGAAAAAGACGCCCGATATTAACCCAGAAAGCCAAGACATCGCCGAGCTTCAAGCTATGGTGAAAGCGTTGATGTCGGAGAAAAAAGAG
TGGCAACAAGAGCGCCAATCCTTAATTGAGCAGTTTAAACTGGCCCTTGACCGTCAATTCGCCAAACGCTCGGAAGCGTTAAAGCCGTATAACGAAGCAC
AGGGTGACCTCTTTAATGAGGCGGAATGTGAAGCGGCGAAATCGGAAGATGAGGTTATCACCACCACAACGACGACAATCAAACGTCGTGGTAAACGTCA
ACCGCTTCCTAAAGACTTGCCACGTGAAACACTCGTACTTGACCTAGATGAGCACGACAAGCAGTGCTCATGCTGCCAACACATTTTGCATAAAATCGGC
GAAGACCGCAGTGAAAAGCTTGAGTTTACGCCCGCCGTCCTAAAAGTGTTGGAATATGTTCGCCCGAAATACGCCTGTCGTCAGTGTGAACAAGAGGCTG
AAAAAAGCCACATTCACCAACAACCCGCACCGATAAGTATCATCCCCAAAAGCTTCGCTACCGAAAGTCTGCTTGCCAACATTATTTTAGGCAAATATCA
GTATGCGCTTCCGCTGTATCGTCAGGAAAGCTTGTTTTCCCAGTCAGGTATCGAGCTATCGAGAACCACCATGGCAAGGTGGGTTATCCAAGTGAGTGAA
AAGTTCAAACCGCTTTATCAGGCACTGAAGACTCACTTACTCGAACAAGTGGTGGTTCAGGCCGATGAAACGCCGCTCAATGTGCTTAAGGAAGATAAGC
AATGTTATATGTGGTTGTACGGCTCGGGAGCCGACTCGCCTGAAGCTAAACTCCCTGACATGAAAAATATCGTCTTGTTCGACTATCAAAACAGTCGCGC
GAGAGTGTGCCCCGTGAACTTCTTGGGTGATTACTCAGGCTATCTGCAAAGTGATGGTTACGCGGCTTATGATGGATTAACCAACGTCACCAATGTCGGT
TGCTTCGCTCATGCACGTCGTAAGTTCATGGAAGCGAAGAAGCTTCAGGGCAAAGGTAAAACAGGTAAAGCGGATGTGGCGTTAGCCAAAATCCAAAAGT
TGTACGCACTTGAATCTCGATTAAAACTGGCTTCGGTCGAAGAGCGTTGGGCAGAAAGACAAGCGCAGGCTAAACCGATGTTGGATGACTTCTATGACTG
GTTAACCACGCAGAAAGTCATCGAATCGAGTCCTCTTGGTAAAGCCATCAAGTACACCTTGGGTCAGTGGCCTAAGCTGATACGCTATGTTGAAGATGGG
CATTTATCTATCGACAACAACCGAGCAGAGCGCGCAATCAAATCGCTGGTCATTGGCAGAAAAAATTGGTTATTCTCGACGAATCCGCTTGGTGCTGATG
CGAGTGCTTTGCTGTATAGCATCATCGAGACGGCGAAAGCGAACGGACTGATACTCTACGATTACATGGTCAAATGTATGAAAGAACTGGCAAAGCCAGA
GCCTGATATTAACTCTCTTCTGCCTTGGAACGTCTCACACTGAAATTAAAACGCCCCGTGGGATTATGGGGCGCATAC
CCAAGAATGGCAAACCTTGTTCGAGCAATACCAATCCAGTCATCTATCACAGCGGGTCTTCTGTGAGCGTAATGGGTTAAGTCTTTCTACTTTTTATGCC
AAACGCCAGCAGTTACAAACTACGGAGTCAGCCAATATCGGTGGCTTTGTTAGAGCAGAAGTTATCGAAAAAACGACTCGCTACCAAGTGGCTCAAGCAC
CCGTCGCCAACATGACGCTATCCATTAATGATGTTGAACTCAGCATTCCCCAGGGAACACCTGTGGCCTACTTAGCCGAGTTAATCGGAGTGCTGTCATG
AAGAGAATGCTTAGTGCACCCAATATTTACTTGTATCGAGAGTTCGTGGACTTTAGAAAATCCATCAATGGATTAGCAGCGATTATCGAGTCCGAAACCG
ATTTGCCGCTAGGTACAGGTGCGCTATTCCTGTTTACCAATAAACAACGCGACAAAGTCAAAGTGTTGTACTGGGATAAAACGGGCTTTGCCCTGTGGTA
CAAACGGTTGGAAAAAGCCAAATTCAAATGGCCCACTCAAGAGAAAAAGCAGGTCTACACCCTGACTCAATTTGACTTAGATAGACTGTTATCTGGCTTC
ACGATAATCGGCCATAAACCGATAAAAATAGACAACTTTACAATGAGTTGAACCCGATAAAAGTGAGTTATACCAAGCCTTGACGTGGTGTTTTTAATAT
AATCAATGCCATGAAAAAGACGCCCGATATTAACCCAGAAAGCCAAGACATCGCCGAGCTTCAAGCTATGGTGAAAGCGTTGATGTCGGAGAAAAAAGAG
TGGCAACAAGAGCGCCAATCCTTAATTGAGCAGTTTAAACTGGCCCTTGACCGTCAATTCGCCAAACGCTCGGAAGCGTTAAAGCCGTATAACGAAGCAC
AGGGTGACCTCTTTAATGAGGCGGAATGTGAAGCGGCGAAATCGGAAGATGAGGTTATCACCACCACAACGACGACAATCAAACGTCGTGGTAAACGTCA
ACCGCTTCCTAAAGACTTGCCACGTGAAACACTCGTACTTGACCTAGATGAGCACGACAAGCAGTGCTCATGCTGCCAACACATTTTGCATAAAATCGGC
GAAGACCGCAGTGAAAAGCTTGAGTTTACGCCCGCCGTCCTAAAAGTGTTGGAATATGTTCGCCCGAAATACGCCTGTCGTCAGTGTGAACAAGAGGCTG
AAAAAAGCCACATTCACCAACAACCCGCACCGATAAGTATCATCCCCAAAAGCTTCGCTACCGAAAGTCTGCTTGCCAACATTATTTTAGGCAAATATCA
GTATGCGCTTCCGCTGTATCGTCAGGAAAGCTTGTTTTCCCAGTCAGGTATCGAGCTATCGAGAACCACCATGGCAAGGTGGGTTATCCAAGTGAGTGAA
AAGTTCAAACCGCTTTATCAGGCACTGAAGACTCACTTACTCGAACAAGTGGTGGTTCAGGCCGATGAAACGCCGCTCAATGTGCTTAAGGAAGATAAGC
AATGTTATATGTGGTTGTACGGCTCGGGAGCCGACTCGCCTGAAGCTAAACTCCCTGACATGAAAAATATCGTCTTGTTCGACTATCAAAACAGTCGCGC
GAGAGTGTGCCCCGTGAACTTCTTGGGTGATTACTCAGGCTATCTGCAAAGTGATGGTTACGCGGCTTATGATGGATTAACCAACGTCACCAATGTCGGT
TGCTTCGCTCATGCACGTCGTAAGTTCATGGAAGCGAAGAAGCTTCAGGGCAAAGGTAAAACAGGTAAAGCGGATGTGGCGTTAGCCAAAATCCAAAAGT
TGTACGCACTTGAATCTCGATTAAAACTGGCTTCGGTCGAAGAGCGTTGGGCAGAAAGACAAGCGCAGGCTAAACCGATGTTGGATGACTTCTATGACTG
GTTAACCACGCAGAAAGTCATCGAATCGAGTCCTCTTGGTAAAGCCATCAAGTACACCTTGGGTCAGTGGCCTAAGCTGATACGCTATGTTGAAGATGGG
CATTTATCTATCGACAACAACCGAGCAGAGCGCGCAATCAAATCGCTGGTCATTGGCAGAAAAAATTGGTTATTCTCGACGAATCCGCTTGGTGCTGATG
CGAGTGCTTTGCTGTATAGCATCATCGAGACGGCGAAAGCGAACGGACTGATACTCTACGATTACATGGTCAAATGTATGAAAGAACTGGCAAAGCCAGA
GCCTGATATTAACTCTCTTCTGCCTTGGAACGTCTCACACTGAAATTAAAACGCCCCGTGGGATTATGGGGCGCATAC
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 321 bp | 106 aa | 81 | 401 | + | No |
AG : IS66 TnpA
ORF sequence :
MVKRRTNQEWQTLFEQYQSSHLSQRVFCERNGLSLSTFYAKRQQLQTTESANIGGFVRAEVIEKTTRYQVAQAPVANMTLSINDVELSIPQGTPVAYLAE
LIGVLS
LIGVLS
Blast result :Comments : STOP codon overlapping/into ORF2-sequenceORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 354 bp | 117 aa | 398 | 751 | + | No |
AG : IS66 TnpB
ORF sequence :
MKRMLSAPNIYLYREFVDFRKSINGLAAIIESETDLPLGTGALFLFTNKQRDKVKVLYWDKTGFALWYKRLEKAKFKWPTQEKKQVYTLTQFDLDRLLSG
FTIIGHKPIKIDNFTMS
FTIIGHKPIKIDNFTMS
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1533 bp | 510 aa | 811 | 2343 | + | No |
Chemistry : DDE
ORF sequence :
MKKTPDINPESQDIAELQAMVKALMSEKKEWQQERQSLIEQFKLALDRQFAKRSEALKPYNEAQGDLFNEAECEAAKSEDEVITTTTTTIKRRGKRQPLP
KDLPRETLVLDLDEHDKQCSCCQHILHKIGEDRSEKLEFTPAVLKVLEYVRPKYACRQCEQEAEKSHIHQQPAPISIIPKSFATESLLANIILGKYQYAL
PLYRQESLFSQSGIELSRTTMARWVIQVSEKFKPLYQALKTHLLEQVVVQADETPLNVLKEDKQCYMWLYGSGADSPEAKLPDMKNIVLFDYQNSRARVC
PVNFLGDYSGYLQSDGYAAYDGLTNVTNVGCFAHARRKFMEAKKLQGKGKTGKADVALAKIQKLYALESRLKLASVEERWAERQAQAKPMLDDFYDWLTT
QKVIESSPLGKAIKYTLGQWPKLIRYVEDGHLSIDNNRAERAIKSLVIGRKNWLFSTNPLGADASALLYSIIETAKANGLILYDYMVKCMKELAKPEPDI
NSLLPWNVSH
KDLPRETLVLDLDEHDKQCSCCQHILHKIGEDRSEKLEFTPAVLKVLEYVRPKYACRQCEQEAEKSHIHQQPAPISIIPKSFATESLLANIILGKYQYAL
PLYRQESLFSQSGIELSRTTMARWVIQVSEKFKPLYQALKTHLLEQVVVQADETPLNVLKEDKQCYMWLYGSGADSPEAKLPDMKNIVLFDYQNSRARVC
PVNFLGDYSGYLQSDGYAAYDGLTNVTNVGCFAHARRKFMEAKKLQGKGKTGKADVALAKIQKLYALESRLKLASVEERWAERQAQAKPMLDDFYDWLTT
QKVIESSPLGKAIKYTLGQWPKLIRYVEDGHLSIDNNRAERAIKSLVIGRKNWLFSTNPLGADASALLYSIIETAKANGLILYDYMVKCMKELAKPEPDI
NSLLPWNVSH
Blast result :
Comments
ISVa12 is 86% (TnpA), 100% (TnpB) and 98% (TnpC : the transposase) aa similar to ISVa9.
References
1] Holm K.O. (2016) Direct submission
2] Austin B. & al (1995) Systematic and Applied Microbiology 18: 285-302
2] Austin B. & al (1995) Systematic and Applied Microbiology 18: 285-302