ISEc70

  • Family IS1595
  • Group IS1595
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
CP015139 ND Escherichia coli
Escherichia coli Ecol_732 pEC732_IMP14
DNA section
IS Length : 1018 bp

Ends


IR Length : 20/24

IRL : cctgattaccaccatgcttcagcccctcaggactatacttaaagtatcct
IRR : cctgaTTACCATCGAGATTCAGCCAGTTTTAGCAATCGCTGTGGCATAGG

Insertion site


Left flankDirect repeatRight flankDR Length
TGATTTAAATCAATCCCTTTAGGTAAATATTG8

DNA sequence

CCTGATTACCACCATGCTTCAGCCCCTCAGGACTATACTTAAAGTATCCTGTTCTGGAGGGTTTAATCATGGCCAAGAACAAGGTTCAGTTTCAAAAAAG
CATGAATATTCATGCCTTTATTCAGAGTTATGGCAGTGAAGAGCGGTGTCAGCAACGGCTGTTTGATATTCGTTGGCCTCAAGGCTTCCTATGTCCAAAC
TGCGGACATACCCACTATTGCAGGCTTCACTCGAGATCGGTTTACCAATGTAATCAGTGTCACCAACAAACCTCGCTGACTGCTGGAACCATTTTTGCCT
ACTCCAAATTGCCTCTCACCATCTGGTTTTTGGCCATCTATCTGATCACTCAAGAGAAAAATGGTGTTTCCGCTCTGGAGTTATCACGTTCGCTTGGCAT
CTCTTACAACGCGACCTGGCGACTTAAGCACAAGTTGATGCAAGCCATGAAAGAGCGTGATGATGAGGTTAAGCTATCAGGCATCATCCAGCTTGATGAT
GCTTATTGGGGCGGAGCTAGAACTGGAAAGGTAGGTCGTGGAGCGGCAGGCAAACGCCCATTTGTTGCAGCCGTATCCCTCACCGAGGAAGGACACCCGA
ACTGCATGCGATTCAGTGCGGTCAATGGCTTCAAGAAAACGGAACTAACTCAATGGGCGAAGAAGCATCTGCAGCCATCATCCTTGGTTGTCTCGGATGG
TTTGGCCTGTTTCCGAGGCATTGAAGAGGCCAATAGTTTTCATCTTGGGATCGTGACAGGAGGAGGGCCTGAAAGTGTCAAACTGCCCTACTTCAAGTGG
GTCAATACCATGATAAGTAACGTAAAGAATTCCATTCATGGAACCTATCACGCGATAAATCAGAAGCATCTGCCACGGTATTTGGCTGAGTTCAGCTTTA
AGTTCAATCACCGATTCAATCTGGAGTGGATGGTAACTGATTTAGCCAGAGCAGGAATTCGAACGGCACCTATGCCACAGCGATTGCTAAAACTGGCTGA
ATCTCGATGGTAATCAGG
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
945 bp314 aa691013+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MAKNKVQFQKSMNIHAFIQSYGSEERCQQRLFDIRWPQGFLCPNCGHTHYCRLHSRSVYQCNQCHQQTSLTAGTIFAYSKLPLTIWFLAIYLITQEKNGV
SALELSRSLGISYNATWRLKHKLMQAMKERDDEVKLSGIIQLDDAYWGGARTGKVGRGAAGKRPFVAAVSLTEEGHPNCMRFSAVNGFKKTELTQWAKKH
LQPSSLVVSDGLACFRGIEEANSFHLGIVTGGGPESVKLPYFKWVNTMISNVKNSIHGTYHAINQKHLPRYLAEFSFKFNHRFNLEWMVTDLARAGIRTA
PMPQRLLKLAESRW

 

Blast result :
Comments
ISEc70 is 75% aa similar to ISPma1.
References
1] Thomas Jové (2016) Direct submission.
2] Stoesser,N., Sheppard,A., Peirano,G., Sebra,R., Lynch,T., Anson,L., Kasarskis,A., Motyl,M., Kazmierczak,K., Crook,D. and Pitout,J. (2016) Direct GenBank submission.