ISAfe18

  • Family IS1595
  • Group ISSod11
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
LVXZ01000081 ND Acidithiobacillus ferrooxidans
Acidithiobacillus ferrooxidans BY0502
DNA section
IS Length : 1111 bp

Ends


IR Length : 22/27

IRL : TATGCTTATCGGGTTGTCTCCCAAGACTACTACCGGTAGGATGTTGGGCA
IRR : CGTGCTTATCGGGTTAGCTCCCAAGCCCGCTAGATGTAGTGGATCGTGAT

Insertion site


Left flankDirect repeatRight flankDR Length
ACGTCTATAACGACGAAGtacatcgcga8

DNA sequence

TATGCTTATCGGGTTGTCTCCCAAGACTACTACCGGTAGGATGTTGGGCATCTGTTCCAATGTGTGTCAACTATGGTTATCGACGTGCTTCAGCGATACG
ACTTGCCGTTTCCGCGCTCGCTTCCAGAGTTCCAGCGACTGTTCTCGAACGAAGCAGCATGCGCGGCCTACCTTGAGCGCGCGAGATGGAGTGATGGATT
CCTCTGTGACCACTGCGGCTCGCCTGATGAGCCGTACCGATTTGCTAATCGCCCCGGCGTTCTCCGGTGTCGCCATTGCAACCGCGATACGAGCCTCACC
GCTGGCACCGTCATGGAGCGCACACACACGCCGCTCTCGGTTTGGTTCTGGGCGGCGTATTTGGTCACCAGTCAGACACCCGGCATATCGGCTGTTCAGT
TCCAGCGGCAACTCGGATTGTCGTGCTACGAAACTGCCTTCCAGATTCTTCACAAGCTGCGCGCCGGCATGGTGCGACCAGATCAGGATTGGATCGGCGG
TAAGCCCGAAGAAGTCGTCGAGGCCGATGAAACCTACGTGGGCGGACGTACGCGCGGCAAGGGGCGCGGCGTCCATGATATGGTCATCGTGGCCGGTGCT
GTCGAAGTTCGTCAGCGCAAGCATACCAGCAGCCTTAACAAGCGGAAGAGTGGGCGGTATGCCGGTCGTGTCAGGCTCGCCCTGGTGCCGGACAGAAGCA
CCAAGTCTTTGGGTGGCTTCATCGAAAGCGCCGTGGCGCCAGGCACCACCATTATCACCGACGACTGGAGCGGCTACGCTGGCCTTGCCAAGCGCAACTA
TCTCCACACAGCTGTCCCGGAACGGGGCGATATGCAAGTCTCCGAATCCTTTCTGCCTATCATCCACCTCGTCTTCTCCAATCTCAAAACCTGGCTGCGC
GGCATCCATCACGGCGTCAGCCAGCAACATCTGCAGGCGTACCTCAATGAGTTCACGTTCCGTTTCAACCGCCGCTTCTACCCCTTCAACGCCTTCCGCT
CCCTGCTTGGCATCGCGGGCGATGTCACCGCGCCAACCTATGCCGAGCTTTATGCCAAAAAATCACGATCCACTACATCTAGCGGGCTTGGGAGCTAACC
CGATAAGCACG
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
1026 bp341 aa731098+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MVIDVLQRYDLPFPRSLPEFQRLFSNEAACAAYLERARWSDGFLCDHCGSPDEPYRFANRPGVLRCRHCNRDTSLTAGTVMERTHTPLSVWFWAAYLVTS
QTPGISAVQFQRQLGLSCYETAFQILHKLRAGMVRPDQDWIGGKPEEVVEADETYVGGRTRGKGRGVHDMVIVAGAVEVRQRKHTSSLNKRKSGRYAGRV
RLALVPDRSTKSLGGFIESAVAPGTTIITDDWSGYAGLAKRNYLHTAVPERGDMQVSESFLPIIHLVFSNLKTWLRGIHHGVSQQHLQAYLNEFTFRFNR
RFYPFNAFRSLLGIAGDVTAPTYAELYAKKSRSTTSSGLGS

 

Blast result :
Comments
ISAfe18 is 91% aa similar to ISCARN100.
References
1] Thomas Jové (2016) Direct submission.
2] Zhou,Z. (2016) Direct GenBank submission.