ISAisp6
- Family IS21
- Group
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| CP000539 | ND | Acidovorax sp. | Pseudomonas pseudoalcaligenes CECT 5344 Acidovorax sp. JS42 |
DNA section
IS Length : 2657 bp
Ends
IR Length : 22/28
IRL : tgttggcgccgaccgaaaactgagccacttttgagggtagtgccgaccca
IRR : tgtccgcgccgatccaaatttgagccaccgtgccgactgaatattgagcc
Comments : no DR
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| GGAAGGCGGG | ttcctcgcgc | 0 | |
| ccgcatgtcc | cccgatatca | 0 | |
| cggacacgat | ccgagaggtc | 0 | |
| gcggcgtggc | ctcgacgaac | 0 | |
| TCCGCCAGCT | CGCGTTGTGC | 0 |
DNA sequence
TGTTGGCGCCGACCGAAAACTGAGCCACTTTTGAGGGTAGTGCCGACCCAAAACTGAGCCAGGTGAACAACCTACTCTGCTGTTTTTTTGTGCAGCAGAG
GACAAGGAGTGATCACCATGGACATGATTGGCAGGATCCGGCATTTGCATGCCCGGAAGAACAAATCGGAGCGCGAGATATCGCGCATGACAGGGCTCTC
GCGCAACACGGTTGCGAAGTGGCTGCACGGGGAAGTGGACGGTCCACCGAAGTACCGGCGCGGCGAGCAGCCCAACAAACTCACGGCGTTCCATGACGTG
CTCAGGCAGGCCTTGAAGGCTGATGCACGTCGGCCCAAACACGAGCGGCGCACAGCCAAGGCGCTGTACGCCGAGATCAGGCGTGCGGGCTATGAGGGTG
GTTACACGCGGGTGACCGACTTCATCCGGGCATGGCGCCAGGGCGAAGGCCAGGGGTCTGCGGGCAACGCCTTCGTGCCGTTGGCCTTCGAGCTGGGCGA
GGCTTTCCAGTTCGACTGGAGCGAGGAAGGCATGGTGATCGGCGGCATCTACTACCGGGTGCAGGTCTCACACATGAAGCTGTGCGCCAGTCGGGCTTTC
TGGCTGGTGGCCTACCCCAGCCAGGGCCACGAGATGCTGTTCGATGCCCACACCCGCAGCTTCGCCGCGCTGGGTGGCGTGGCGCGCCGGGGCATCTATG
ACAACATGAAGACTGCGGTAGACAAGGTCAAGAAGGGCAAGGGGCGCATCGTCAACGAGCGCTTTGCCACCATGTGCGCGCATTACCTGTACGACCCGGA
CTTCTGCAACGTGGCCAGCGGCTGGGAGAAAGGGGTGGTGGAGAAGAACGTGCAAGACAGCCGCCGGCGCATCTGGATCGACGCGGCCAAGATCAAGTTC
GGCAGCTTTACCGAACTCAACGCCTGGCTGGCCCAGCGCTGCCGCGCGTTGTGGGACGAGGTGCGCCACCCCGAGCACACCCAGTTCAGCGTGGCCGAGA
TGCTCGAACACGAGCGCGCGCACCTCATGCCCATGCCCGTGCCGTTCGATGGCTACGTCGAGAAACCCGCTCGGGTCACCAGCACCTGTCTGGTGTCGGT
GGCGCGCAACCGCTATTCGGTGCCGTGCGAACGGGTGGGCCAGATGGTCAGTACGCGGTTGTATCCGGGCAGCGTCGCCATCGTGGCCGACGACACCATC
GTGGCCCGCCACGACCGGCTGAGCAACGCGGGCGAGACCCGGTACGACTGGCAGCACTACATCCCGCTGCTGCAGAGAAAGCCCGGTGCCCTGAGGAACG
GCGCGCCCTTTGCCGACATGCCCGAGCCGCTGCAGCGGCTGCGCCGTGGGCTGCTGCGCAACCCGGGCGGCGACCGGGTGATGGCGCAGGTGTTGGCCAT
TGTGCTCAGCGCCGGTCTGGACGCGGTGCTGGTGGCCATCGAGCTGGCGCTGGAGAGTGGGCCGCCTGGCAAGGTCAGTGTGGAGCACGTGGTCAACGTG
CTGAACCGGCTCAACGCGCAGCCGGTGCCGCCCACGGCGGCCACGCTGCTGAGAGCGACCACGCCGCCGCTGGCGAACACGGCGCGCTACGACAGCCTGC
GCTCGGACACCGTGGGTGCCATCACCGAAGGAGCCGACCATGAAGACTGATGTGCTGGTCGAACTCAAGGCACTGCGCCTGCACGGCATGGCCGGCGCGT
GGGCCGATCTGGTCGAGCAAGGGGGTGGCGAGGCAGGCGGTGTGGGCATCGAGTCCTCGCGCTGGCTGATCGAGCACCTGTTGCAGGCCGAGGGTACGGA
CCGCGCGATGCGCTCGGTCAGCCACCAGATGCACGCGGCGAAGTTCCCGGTGCACCGCGACATGGCGGGCTTTGACTTCGAGGTCTCGCCGGTGGACCGC
AACCTGGTGGAGACTCTGGCCACCACGGCCTTCACCGACGAGGCGCACAACGTGGTGCTGGTGGGCGGACCGGGCACAGGCAAGACCCATCTGGCCACAG
CAATCGGCGTCTCTGGCATCACCCGGCACGGCAAGCGGGTGCGCTTCTATTCCACCGTCGATCTGGTCAACGCACTGGAGCAGGAGAAGGCCCAAGGCAA
GGCCGGTCGCATCGCTACCAGCCTGCTGCGCATGGATCTGGTGATCCTCGATGAACTGGGCTACCTGCCGTTCAGCCAGGCCGGTGGGGCGCTGCTGTTC
CACCTGCTGAGCAAGCTGTACGAGCACACCAGCGTGATGATCACGAGCAACCTGGACTTCGCAGAATGGTCCAGCGTGTTCGGCGACGCGAAGATGACCA
CCGCGTTGCTGGACCGACTCACGCACCACTGCCACATCATGGAGACCGGCAACGAATCGATCCGATTCAGTCGCAGCACGGCCGCAGCCAAGAAGCGCAT
CAAGGCCCGAGAGCAGGCGCGCAAGGGAGCCAAAACCGAAGCTGGAGACGAACCCTTCTGATCGGAAACGGTGATGGGCAAGCCGCTACGGGCTACGCCC
TGCGCGGCTTGCCCATCGCATTTAACAACACGCCAAGGAGACCAGCAAACCAGCACAGCCTCTACACTTATCCACAGCCGACCCTCTCAAAGTCGGCTTC
CACCCCTGGCTCAATATTCAGTCGGCACGGTGGCTCAAATTTGGATCGGCGCGGACA
GACAAGGAGTGATCACCATGGACATGATTGGCAGGATCCGGCATTTGCATGCCCGGAAGAACAAATCGGAGCGCGAGATATCGCGCATGACAGGGCTCTC
GCGCAACACGGTTGCGAAGTGGCTGCACGGGGAAGTGGACGGTCCACCGAAGTACCGGCGCGGCGAGCAGCCCAACAAACTCACGGCGTTCCATGACGTG
CTCAGGCAGGCCTTGAAGGCTGATGCACGTCGGCCCAAACACGAGCGGCGCACAGCCAAGGCGCTGTACGCCGAGATCAGGCGTGCGGGCTATGAGGGTG
GTTACACGCGGGTGACCGACTTCATCCGGGCATGGCGCCAGGGCGAAGGCCAGGGGTCTGCGGGCAACGCCTTCGTGCCGTTGGCCTTCGAGCTGGGCGA
GGCTTTCCAGTTCGACTGGAGCGAGGAAGGCATGGTGATCGGCGGCATCTACTACCGGGTGCAGGTCTCACACATGAAGCTGTGCGCCAGTCGGGCTTTC
TGGCTGGTGGCCTACCCCAGCCAGGGCCACGAGATGCTGTTCGATGCCCACACCCGCAGCTTCGCCGCGCTGGGTGGCGTGGCGCGCCGGGGCATCTATG
ACAACATGAAGACTGCGGTAGACAAGGTCAAGAAGGGCAAGGGGCGCATCGTCAACGAGCGCTTTGCCACCATGTGCGCGCATTACCTGTACGACCCGGA
CTTCTGCAACGTGGCCAGCGGCTGGGAGAAAGGGGTGGTGGAGAAGAACGTGCAAGACAGCCGCCGGCGCATCTGGATCGACGCGGCCAAGATCAAGTTC
GGCAGCTTTACCGAACTCAACGCCTGGCTGGCCCAGCGCTGCCGCGCGTTGTGGGACGAGGTGCGCCACCCCGAGCACACCCAGTTCAGCGTGGCCGAGA
TGCTCGAACACGAGCGCGCGCACCTCATGCCCATGCCCGTGCCGTTCGATGGCTACGTCGAGAAACCCGCTCGGGTCACCAGCACCTGTCTGGTGTCGGT
GGCGCGCAACCGCTATTCGGTGCCGTGCGAACGGGTGGGCCAGATGGTCAGTACGCGGTTGTATCCGGGCAGCGTCGCCATCGTGGCCGACGACACCATC
GTGGCCCGCCACGACCGGCTGAGCAACGCGGGCGAGACCCGGTACGACTGGCAGCACTACATCCCGCTGCTGCAGAGAAAGCCCGGTGCCCTGAGGAACG
GCGCGCCCTTTGCCGACATGCCCGAGCCGCTGCAGCGGCTGCGCCGTGGGCTGCTGCGCAACCCGGGCGGCGACCGGGTGATGGCGCAGGTGTTGGCCAT
TGTGCTCAGCGCCGGTCTGGACGCGGTGCTGGTGGCCATCGAGCTGGCGCTGGAGAGTGGGCCGCCTGGCAAGGTCAGTGTGGAGCACGTGGTCAACGTG
CTGAACCGGCTCAACGCGCAGCCGGTGCCGCCCACGGCGGCCACGCTGCTGAGAGCGACCACGCCGCCGCTGGCGAACACGGCGCGCTACGACAGCCTGC
GCTCGGACACCGTGGGTGCCATCACCGAAGGAGCCGACCATGAAGACTGATGTGCTGGTCGAACTCAAGGCACTGCGCCTGCACGGCATGGCCGGCGCGT
GGGCCGATCTGGTCGAGCAAGGGGGTGGCGAGGCAGGCGGTGTGGGCATCGAGTCCTCGCGCTGGCTGATCGAGCACCTGTTGCAGGCCGAGGGTACGGA
CCGCGCGATGCGCTCGGTCAGCCACCAGATGCACGCGGCGAAGTTCCCGGTGCACCGCGACATGGCGGGCTTTGACTTCGAGGTCTCGCCGGTGGACCGC
AACCTGGTGGAGACTCTGGCCACCACGGCCTTCACCGACGAGGCGCACAACGTGGTGCTGGTGGGCGGACCGGGCACAGGCAAGACCCATCTGGCCACAG
CAATCGGCGTCTCTGGCATCACCCGGCACGGCAAGCGGGTGCGCTTCTATTCCACCGTCGATCTGGTCAACGCACTGGAGCAGGAGAAGGCCCAAGGCAA
GGCCGGTCGCATCGCTACCAGCCTGCTGCGCATGGATCTGGTGATCCTCGATGAACTGGGCTACCTGCCGTTCAGCCAGGCCGGTGGGGCGCTGCTGTTC
CACCTGCTGAGCAAGCTGTACGAGCACACCAGCGTGATGATCACGAGCAACCTGGACTTCGCAGAATGGTCCAGCGTGTTCGGCGACGCGAAGATGACCA
CCGCGTTGCTGGACCGACTCACGCACCACTGCCACATCATGGAGACCGGCAACGAATCGATCCGATTCAGTCGCAGCACGGCCGCAGCCAAGAAGCGCAT
CAAGGCCCGAGAGCAGGCGCGCAAGGGAGCCAAAACCGAAGCTGGAGACGAACCCTTCTGATCGGAAACGGTGATGGGCAAGCCGCTACGGGCTACGCCC
TGCGCGGCTTGCCCATCGCATTTAACAACACGCCAAGGAGACCAGCAAACCAGCACAGCCTCTACACTTATCCACAGCCGACCCTCTCAAAGTCGGCTTC
CACCCCTGGCTCAATATTCAGTCGGCACGGTGGCTCAAATTTGGATCGGCGCGGACA
Protein section
ORF number : 2
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1533 bp | 510 aa | 118 | 1650 | + | No |
Chemistry : DDE
ORF sequence :
MDMIGRIRHLHARKNKSEREISRMTGLSRNTVAKWLHGEVDGPPKYRRGEQPNKLTAFHDVLRQALKADARRPKHERRTAKALYAEIRRAGYEGGYTRVT
DFIRAWRQGEGQGSAGNAFVPLAFELGEAFQFDWSEEGMVIGGIYYRVQVSHMKLCASRAFWLVAYPSQGHEMLFDAHTRSFAALGGVARRGIYDNMKTA
VDKVKKGKGRIVNERFATMCAHYLYDPDFCNVASGWEKGVVEKNVQDSRRRIWIDAAKIKFGSFTELNAWLAQRCRALWDEVRHPEHTQFSVAEMLEHER
AHLMPMPVPFDGYVEKPARVTSTCLVSVARNRYSVPCERVGQMVSTRLYPGSVAIVADDTIVARHDRLSNAGETRYDWQHYIPLLQRKPGALRNGAPFAD
MPEPLQRLRRGLLRNPGGDRVMAQVLAIVLSAGLDAVLVAIELALESGPPGKVSVEHVVNVLNRLNAQPVPPTAATLLRATTPPLANTARYDSLRSDTVG
AITEGADHED
DFIRAWRQGEGQGSAGNAFVPLAFELGEAFQFDWSEEGMVIGGIYYRVQVSHMKLCASRAFWLVAYPSQGHEMLFDAHTRSFAALGGVARRGIYDNMKTA
VDKVKKGKGRIVNERFATMCAHYLYDPDFCNVASGWEKGVVEKNVQDSRRRIWIDAAKIKFGSFTELNAWLAQRCRALWDEVRHPEHTQFSVAEMLEHER
AHLMPMPVPFDGYVEKPARVTSTCLVSVARNRYSVPCERVGQMVSTRLYPGSVAIVADDTIVARHDRLSNAGETRYDWQHYIPLLQRKPGALRNGAPFAD
MPEPLQRLRRGLLRNPGGDRVMAQVLAIVLSAGLDAVLVAIELALESGPPGKVSVEHVVNVLNRLNAQPVPPTAATLLRATTPPLANTARYDSLRSDTVG
AITEGADHED
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 822 bp | 257 aa | 1640 | 2461 | + | No |
AG : IS21 helper
ORF sequence :
MKTDVLVELKALRLHGMAGAWADLVEQGGGEAGGVGIESSRWLIEHLLQAEGTDRAMRSVSHQMHAAKFPVHRDMAGFDFEVSPVDRNLVETLATTAFTD
EAHNVVLVGGPGTGKTHLATAIGVSGITRHGKRVRFYSTVDLVNALEQEKAQGKAGRIATSLLRMDLVILDELGYLPFSQAGGALLFHLLSKLYEHTSVM
ITSNLDFAEWSSVFGDAKMTTALLDRLTHHCHIMETGNESIRFSRSTAAAKKRIKAREQARKGAKTEAGDEPF
EAHNVVLVGGPGTGKTHLATAIGVSGITRHGKRVRFYSTVDLVNALEQEKAQGKAGRIATSLLRMDLVILDELGYLPFSQAGGALLFHLLSKLYEHTSVM
ITSNLDFAEWSSVFGDAKMTTALLDRLTHHCHIMETGNESIRFSRSTAAAKKRIKAREQARKGAKTEAGDEPF
Blast result :
Comments
ISAisp6 is 98% aa (ORF1 : the transposase) and 97% aa (ORF2 : IS21 helper of transposition) similar to ISThsp19.
Additionnal accession number : HG916826.
Additionnal accession number : HG916826.
References
1] Thomas Jové (2016) Direct submission.
2] Copeland,A., Lucas,S., Lapidus,A., Barry,K., Detter,J.C., Glavina del Rio,T., Dalin,E., Tice,H., Pitluck,S., Chertkov,O., Brettin,T., Bruce,D., Han,C., Tapia,R., Gilna,P., Schmutz,J., Larimer,F., Land,M., Hauser,L., Kyrpides,N., Kim,E., Stahl,D. and Richardson,P. (2006) Direct GenBank submission.
3] Wibberg,D., Puehler,A. and Schlueter,A. (2013) Direct GenBank submission.
2] Copeland,A., Lucas,S., Lapidus,A., Barry,K., Detter,J.C., Glavina del Rio,T., Dalin,E., Tice,H., Pitluck,S., Chertkov,O., Brettin,T., Bruce,D., Han,C., Tapia,R., Gilna,P., Schmutz,J., Larimer,F., Land,M., Hauser,L., Kyrpides,N., Kim,E., Stahl,D. and Richardson,P. (2006) Direct GenBank submission.
3] Wibberg,D., Puehler,A. and Schlueter,A. (2013) Direct GenBank submission.