ISAlde1
- Family IS21
- Group
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| CP002657 | ND | Alicycliphilus denitrificans | Alicycliphilus denitrificans K601 |
DNA section
IS Length : 2501 bp
Ends
IR Length : 24/28
IRL : tgttgatttccacgcagaagtgacccactaacccccgggatttccatcca
IRR : tgttgatttccatgcaaagctgacccaccatttccatcgaatcttgaccc
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| AAGTCGTCGCGCGTCGTGCA | CCTTGGGAGCCCACAGGCCA | 0 |
DNA sequence
TGTTGATTTCCACGCAGAAGTGACCCACTAACCCCCGGGATTTCCATCCAAACCTGACCCACGTACTAACCCTAACCTGCTGCTTTGCTGAGCAGCAGGA
GACCAGGAGTGATAGACGTGGCAACACTGAGTGTCATCAGGCGCTGGGCCCTGCGAGAGCAGCTGTCCATCCGGGAGATAGCCCGCCGCACGGGCCTCTC
GCGCAACACCATCCGCAAGTACCTGCGCGTAGGCGAGGCCGAGCCGCACTATGCCAAGCGGGTCAGTCCATCCAAGCTCGATCCCTTCGCCTTGAAGCTC
GCTGGCTGGCTCAAGACGGAAGCTGGCCGATCCCGCAAGCAGCGCCGCACCGTCAAGCAGATGTACGTGGATCTGCAGGCGCTCGGTTATGGCGGCTCCT
ACAACCGCGTAGCGGCCTTTGCCCGCCTCTGGCACGAGCAGCGCCTTGTGGCCCAGCAGACCACTGGCCGCGGCACCTTCGTTCCCCTGGCCTTCGGTCC
GGGTGAGGCCTTCCAGTTCGACTGGAGCGAGGACTGGGCTGTTCTCGCCGGCGTGCGCACCAAGCTGCAGGTAGCCCACTTCAAGCTCAGCCACAGCCGA
GCGTTCTACCTGCGCGCCTATCCGCTGCAAACGCACGAGATGCTGTTCGATGCCCACAACCATGCATTCGCAGTCCTGGGCGGTGTGCCCCGCCGTGGCA
TCTACGACAACATGCGCACCGCCGTAGACCGGGTGCGCCGTGGCAAGGAGCGCGACGTCAATGCGCGCTTTGCGGCCATGGTCAGCCACTTCCTGTTCGA
GGCCGAGTTCTGCAATCCTGCTTCGGGCTGGGAGAAGGGCCAGGTGGAGAAGAACGTGCGCGATGCCCGCCATCGCCTGTGGCAGGTGGTGCCGCCATTC
CTAAGCCTGTCCGATCTCAATGCCTGGCTTGAAGAGCGCTGCGTGGCGCTGTGGCACGAGATCGAGCACGGCAAGCTGCCGGGCACCGTCGCGGATGTCT
GGGCTCAAGAGAAGGCAACCTTGATGCCGATGCCCCGGCCCTTCGATGGCTTTGTGGAGCACACCAAGCGCGTCTCACCCACCTGCTTGGTCCACTTCGA
GCGCAATCGCTACAGCGTGCCGGCGCCGTATGCCAATCGACCGGTGAGCCTGCGGGTCTATGCCGATCGCCTGGTGGTCGCTGCCGAAGGCCAGATCGTG
TGCGAGCATCAGCGCCTGATCGAGCGTAACCACCATGGTGCGGGACAGACCGTGTACGACTGGCGCCACTACCTGGCAGTGCTGCAGCGCAAGCCCGGGG
CCTTGCGCAATGGTGCTCCATTCCTGGAACTGCCAGCAGCCTTCAAGCGCCTGCAGGCCGCCCTCTTGAAGCAGCCAGGCGGCGACCGAGAGATGGTGGA
GGTTCTGGCTCTGGTGCTGCACCACGATGAACAAGCTGTGCTCGCTGCAGTGGAGTTGGCACTGGAGTCAGGGGCTGCCAGCAAGACCCACATCCTGAAC
GTGCTGCACCGCCTGCTGGACGGTAAGCCCGCCCCAGCACCGGTCACCTCGCCCCAGGCCCTCAAGCTGTCTGTGGAACCCCAGGCCAACGTGCTGCGCT
ATGACCAATTGAGGGAGGTGCGCTATGCGTCATGACCCTGCCATCGCTTCCATCGTGATCATGCTGCGCGAGCTCAAGATGCACGGCATGGCCCAGGCGG
TTGCGGAACTGGCTGAGCAAGGTGCGCCGGCCTTCGACGCAGCGCAGCCCATCCTGTCCCAACTGCTCAAGGCCGAGACCGCCGAGCGGGAGGTGCGCTC
TGTGGCCTACCAACTCAAGGTGGCCAGGTTCCCTGCGTACCGGGACCTGGCTGGGTTTGACTTCAGCCACAGCGAGGTGAACGAGGCATTGGTACGCCAG
TTGCACCGCTGCGAATTCTTGGAGAACGCCAACAACGTGGTGCTGGTGGGTGGACCGGGTACGGGCAAGACCCACATCGCCACAGCCCTCGGGGTGCAAG
CCATTGAGCACCATCACCGCAGGGTGCGGTTCTTCTCCACGGTGGAGCTGGTCAATGCACTGGAAGAGGAGAAGGCCCAGGGCAAGCCGGGGCAGATCGC
GCACCGCCTAGCCTATGCCGATCTGGTGATCCTGGATGAACTGGGCTACCTGCCATTCAGCACGTCAGGAGGAGCCTTGCTGTTCCACCTGCTGTCCAAG
CTGTACGAGCGCACGAGCGTCGTGATCACCACCAACCTGAGCTTTAGCGAGTGGGCCAATGTGTTCGGAGATGCCAAGATGACCACGGCGCTGCTGGACC
GGCTCACGCATCACTGCCATATTCTTGAGACCGGCAATGACAGCTACCGGTTCAAGAATAGCTCCGCACAGCAACCACCACAGACCACCAAGAAGGAGAA
GGCGACCAAGAACTTATCCACAACGTGAGCGTGAAGCTCACGAACCAGGGTGGGTCAAGATTCGATGGAAATGGTGGGTCAGCTTTGCATGGAAATCAAC
A
GACCAGGAGTGATAGACGTGGCAACACTGAGTGTCATCAGGCGCTGGGCCCTGCGAGAGCAGCTGTCCATCCGGGAGATAGCCCGCCGCACGGGCCTCTC
GCGCAACACCATCCGCAAGTACCTGCGCGTAGGCGAGGCCGAGCCGCACTATGCCAAGCGGGTCAGTCCATCCAAGCTCGATCCCTTCGCCTTGAAGCTC
GCTGGCTGGCTCAAGACGGAAGCTGGCCGATCCCGCAAGCAGCGCCGCACCGTCAAGCAGATGTACGTGGATCTGCAGGCGCTCGGTTATGGCGGCTCCT
ACAACCGCGTAGCGGCCTTTGCCCGCCTCTGGCACGAGCAGCGCCTTGTGGCCCAGCAGACCACTGGCCGCGGCACCTTCGTTCCCCTGGCCTTCGGTCC
GGGTGAGGCCTTCCAGTTCGACTGGAGCGAGGACTGGGCTGTTCTCGCCGGCGTGCGCACCAAGCTGCAGGTAGCCCACTTCAAGCTCAGCCACAGCCGA
GCGTTCTACCTGCGCGCCTATCCGCTGCAAACGCACGAGATGCTGTTCGATGCCCACAACCATGCATTCGCAGTCCTGGGCGGTGTGCCCCGCCGTGGCA
TCTACGACAACATGCGCACCGCCGTAGACCGGGTGCGCCGTGGCAAGGAGCGCGACGTCAATGCGCGCTTTGCGGCCATGGTCAGCCACTTCCTGTTCGA
GGCCGAGTTCTGCAATCCTGCTTCGGGCTGGGAGAAGGGCCAGGTGGAGAAGAACGTGCGCGATGCCCGCCATCGCCTGTGGCAGGTGGTGCCGCCATTC
CTAAGCCTGTCCGATCTCAATGCCTGGCTTGAAGAGCGCTGCGTGGCGCTGTGGCACGAGATCGAGCACGGCAAGCTGCCGGGCACCGTCGCGGATGTCT
GGGCTCAAGAGAAGGCAACCTTGATGCCGATGCCCCGGCCCTTCGATGGCTTTGTGGAGCACACCAAGCGCGTCTCACCCACCTGCTTGGTCCACTTCGA
GCGCAATCGCTACAGCGTGCCGGCGCCGTATGCCAATCGACCGGTGAGCCTGCGGGTCTATGCCGATCGCCTGGTGGTCGCTGCCGAAGGCCAGATCGTG
TGCGAGCATCAGCGCCTGATCGAGCGTAACCACCATGGTGCGGGACAGACCGTGTACGACTGGCGCCACTACCTGGCAGTGCTGCAGCGCAAGCCCGGGG
CCTTGCGCAATGGTGCTCCATTCCTGGAACTGCCAGCAGCCTTCAAGCGCCTGCAGGCCGCCCTCTTGAAGCAGCCAGGCGGCGACCGAGAGATGGTGGA
GGTTCTGGCTCTGGTGCTGCACCACGATGAACAAGCTGTGCTCGCTGCAGTGGAGTTGGCACTGGAGTCAGGGGCTGCCAGCAAGACCCACATCCTGAAC
GTGCTGCACCGCCTGCTGGACGGTAAGCCCGCCCCAGCACCGGTCACCTCGCCCCAGGCCCTCAAGCTGTCTGTGGAACCCCAGGCCAACGTGCTGCGCT
ATGACCAATTGAGGGAGGTGCGCTATGCGTCATGACCCTGCCATCGCTTCCATCGTGATCATGCTGCGCGAGCTCAAGATGCACGGCATGGCCCAGGCGG
TTGCGGAACTGGCTGAGCAAGGTGCGCCGGCCTTCGACGCAGCGCAGCCCATCCTGTCCCAACTGCTCAAGGCCGAGACCGCCGAGCGGGAGGTGCGCTC
TGTGGCCTACCAACTCAAGGTGGCCAGGTTCCCTGCGTACCGGGACCTGGCTGGGTTTGACTTCAGCCACAGCGAGGTGAACGAGGCATTGGTACGCCAG
TTGCACCGCTGCGAATTCTTGGAGAACGCCAACAACGTGGTGCTGGTGGGTGGACCGGGTACGGGCAAGACCCACATCGCCACAGCCCTCGGGGTGCAAG
CCATTGAGCACCATCACCGCAGGGTGCGGTTCTTCTCCACGGTGGAGCTGGTCAATGCACTGGAAGAGGAGAAGGCCCAGGGCAAGCCGGGGCAGATCGC
GCACCGCCTAGCCTATGCCGATCTGGTGATCCTGGATGAACTGGGCTACCTGCCATTCAGCACGTCAGGAGGAGCCTTGCTGTTCCACCTGCTGTCCAAG
CTGTACGAGCGCACGAGCGTCGTGATCACCACCAACCTGAGCTTTAGCGAGTGGGCCAATGTGTTCGGAGATGCCAAGATGACCACGGCGCTGCTGGACC
GGCTCACGCATCACTGCCATATTCTTGAGACCGGCAATGACAGCTACCGGTTCAAGAATAGCTCCGCACAGCAACCACCACAGACCACCAAGAAGGAGAA
GGCGACCAAGAACTTATCCACAACGTGAGCGTGAAGCTCACGAACCAGGGTGGGTCAAGATTCGATGGAAATGGTGGGTCAGCTTTGCATGGAAATCAAC
A
Protein section
ORF number : 2
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1527 bp | 508 aa | 109 | 1635 | + | No |
Chemistry : DDE
ORF sequence :
MIDVATLSVIRRWALREQLSIREIARRTGLSRNTIRKYLRVGEAEPHYAKRVSPSKLDPFALKLAGWLKTEAGRSRKQRRTVKQMYVDLQALGYGGSYNR
VAAFARLWHEQRLVAQQTTGRGTFVPLAFGPGEAFQFDWSEDWAVLAGVRTKLQVAHFKLSHSRAFYLRAYPLQTHEMLFDAHNHAFAVLGGVPRRGIYD
NMRTAVDRVRRGKERDVNARFAAMVSHFLFEAEFCNPASGWEKGQVEKNVRDARHRLWQVVPPFLSLSDLNAWLEERCVALWHEIEHGKLPGTVADVWAQ
EKATLMPMPRPFDGFVEHTKRVSPTCLVHFERNRYSVPAPYANRPVSLRVYADRLVVAAEGQIVCEHQRLIERNHHGAGQTVYDWRHYLAVLQRKPGALR
NGAPFLELPAAFKRLQAALLKQPGGDREMVEVLALVLHHDEQAVLAAVELALESGAASKTHILNVLHRLLDGKPAPAPVTSPQALKLSVEPQANVLRYDQ
LREVRYAS*
VAAFARLWHEQRLVAQQTTGRGTFVPLAFGPGEAFQFDWSEDWAVLAGVRTKLQVAHFKLSHSRAFYLRAYPLQTHEMLFDAHNHAFAVLGGVPRRGIYD
NMRTAVDRVRRGKERDVNARFAAMVSHFLFEAEFCNPASGWEKGQVEKNVRDARHRLWQVVPPFLSLSDLNAWLEERCVALWHEIEHGKLPGTVADVWAQ
EKATLMPMPRPFDGFVEHTKRVSPTCLVHFERNRYSVPAPYANRPVSLRVYADRLVVAAEGQIVCEHQRLIERNHHGAGQTVYDWRHYLAVLQRKPGALR
NGAPFLELPAAFKRLQAALLKQPGGDREMVEVLALVLHHDEQAVLAAVELALESGAASKTHILNVLHRLLDGKPAPAPVTSPQALKLSVEPQANVLRYDQ
LREVRYAS*
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 804 bp | 267 aa | 1625 | 2428 | + | No |
AG : IS21 helper
ORF sequence :
MRHDPAIASIVIMLRELKMHGMAQAVAELAEQGAPAFDAAQPILSQLLKAETAEREVRSVAYQLKVARFPAYRDLAGFDFSHSEVNEALVRQLHRCEFLE
NANNVVLVGGPGTGKTHIATALGVQAIEHHHRRVRFFSTVELVNALEEEKAQGKPGQIAHRLAYADLVILDELGYLPFSTSGGALLFHLLSKLYERTSVV
ITTNLSFSEWANVFGDAKMTTALLDRLTHHCHILETGNDSYRFKNSSAQQPPQTTKKEKATKNLSTT
NANNVVLVGGPGTGKTHIATALGVQAIEHHHRRVRFFSTVELVNALEEEKAQGKPGQIAHRLAYADLVILDELGYLPFSTSGGALLFHLLSKLYERTSVV
ITTNLSFSEWANVFGDAKMTTALLDRLTHHCHILETGNDSYRFKNSSAQQPPQTTKKEKATKNLSTT
Blast result :
Comments
ISAlde1 is 95% (transposase) and 96% (IS21 helper) aa similar to ISThsp10.
References
1] Thomas Jové (2016) Direct submission.
2] Oosterkamp MJ , Veuskens T, Plugge CM, Langenhoff AA, Gerritse J, van Berkel WJ, Pieper DH, Junca H, Goodwin LA, Daligault HE, Bruce DC, Detter JC, Tapia R, Han CS, Land ML, Hauser LJ, Smidt H, Stams AJ. (2011) J Bacteriol. Sep;193(18):5028-9.
2] Oosterkamp MJ , Veuskens T, Plugge CM, Langenhoff AA, Gerritse J, van Berkel WJ, Pieper DH, Junca H, Goodwin LA, Daligault HE, Bruce DC, Detter JC, Tapia R, Han CS, Land ML, Hauser LJ, Smidt H, Stams AJ. (2011) J Bacteriol. Sep;193(18):5028-9.