ISAlsp1
- Family IS21
- Group
Isoform Synonym(s)
Accession number | Transposition | Origin | Host |
---|---|---|---|
AF109074 | ND | Alcaligenes sp. | Alcaligenes sp. O-1 |
DNA section
IS Length : 2501 bp
Ends
IR Length : 24/28
IRL : tgttgatttccacgcagaagtgacccactgacccccaggatttccatcta
IRR : tgttgatttccatgcaaacctgacccaccatttccatcgaatcttgaccc
Insertion site
Left flank | Direct repeat | Right flank | DR Length |
---|---|---|---|
gggcacggat | ttgagggcca | 0 |
DNA sequence
TGTTGATTTCCACGCAGAAGTGACCCACTGACCCCCAGGATTTCCATCTAAACCTGACCCACGTACTAACCCTAACCTGCTGCTTCTTTGAGCAGCAGGA
GACCAGGAGTGATAGACGTGGCAACACTGAGTGTCATCAGGCGCTGGGCCCTGCGAGAGCAGCTGTCCATCAGGGAGATCGCCCGCCGCACGGGCGTCTC
GCGCAACACCATCCGCAAGTACCTGCGAGCAGGCGAGGCCGAGCCGCGCTACCCCAAGCGGGTCAGCCGTTCCGGGCTCGATCCCTTTGCCTTGAAGCTC
TCGGGCTGGCTCAAGACAGAAGCAGGCCGATCCCGTAAGCAGCGGCGCACCGTCAAGCAGATGCACGCCGATCTGCAGGCCCTGGGCTATACCGGCTCCT
ACAACCGGGTTGCCGCCTTTGCCCGTCTCTGGCAAGAGCAGCGCGTGGTGGCCCAGCAGACCACCGGCCGCGGCACCTTCGTTCCCCTGGCCTTCGGCCC
GGGCGAGGCCTTCCAGTTCGACTGGAGCGAAGACTGGGCAGTGCTGGCCGGAGTGCGCACCAAGCTGCAGGTAGCCCACTTCAAGCTCAGCCACAGCCGA
GCGTTCTACCTGCGCGCCTATCCGCTGCAGACGCACGAGATGCTGTTCGATGCCCACAACCATGCATTCGCAGTCCTGGGCGGTGTGCCCCGCCGTGGCA
TCTACGACAACATGCGCACCGCTGTAGACCGGGTGCGCCGTGGCAAGGAACGCGACGTCAATGCGCGCTTTGCGGCCATGGTCAGTCACTTCCTGTTCGA
GGCCGAGTTCTGCAACCCTGCCTCCGGCTGGGAGAAGGGCCAGGTGGAGAAGAATGTGCGCGATGCCCGTCATCGCCTGTGGCAGGTCGTTCCGCCATTC
CGTGGCCTGACGGAGCTCAATGCCTGGCTTGAGGAGCGCTGCGTGGCCTTATGGAATGAGATCGAGCATGGCAAGCTGCCGGGCACCGTCGCGGACGTCT
GGGCCGCGGAGCGGGCTGCCTTGATGCCGGTGCCCAGGACCTTCGATGGCTTTGTGGAGCACACAAAGCGGGTCTCGCCCACCTGCCTGGTCCACTTCGA
GCGCAATCGCTACAGCGTACCAGCGCCGTATGCCAATCGGCCGGTGAGCCTGCGGGTCTACGCGGATCGCCTAGTGGTCGCGGCCGAAGGCCAGATCGTC
TGCGAGCACCGGCGCCTGATCGAACGCAACCACCATGGTGCGGGACAGACCGTCTACGACTGGCGCCACTACCTGGCGGTGTTGCAGCGCAAGCCCGGGG
CCTTGCGCAATGGCGCGCCGTTCCTGGAGTTGCCGCCCGCCTTCAAGCGCCTGCAGGCCACCTTGCTCAAGCAACCCGGCGGCGACCGGGAGATGGTGGA
GGTTCTGGCACTGGTGCTGCACCACGATGAACAAGCTGTGCTCGCCGCAGTGGAGCTGGCCATAGAGTCGGGAGCTGCCAGCAAGACCCACATCCTGAAC
GTGCTCCACCGCCTGCTGGACGGCAAGCCCGCTCCAGCACCGGTCACCTCGCCCCAGGCTCTCAAGCTGGCGGTGGAACCCCAGGCCAATGTGCTTCGCT
ACGACCAATTGAGGGAGGTGCGCTATGCGTCATGACCCTGCCATCGCTTCCATCGTGATCATGCTGCGCGAGCTCAAGATGCACGGCATGGCCCAGGCGG
TTGCGGAACTGGCCGAGCAAGGTGCGCCGGCCTTCGACGCAGCACAGCCCATCCTGTCCCAACTGCTCAAGGCCGAGACCGCCGAGCGGGAGGTGCGCTC
AGTGGCTTACCAATTGAAGGTGGCCAGGTTCCCTGCGTACCGGGACATGGCGGGCTTTGACTTCAGCCTCAGCGAGGTGAACGAGGCACTGGTGCGCCAG
CTGCACCGCTGCGAATTCTTGGAGAACGCCAACAACGTGGTGCTGGTGGGTGGACCGGGTACGGGCAAGACCCACATTGCCACAGCCCTCGGAGTGCAAG
CCATTGAGCACCATCACCGCAGGGTGCGGTTCTTCTCCACGGTGGAGCTGGTCAATGCGTTGGAGGAGGAGAAGGCCAACGGCAAGCCCGGGCAGTTGGC
CCACCGCCTGGCCTATGCCGATCTGGTGATCCTGGATGAACTGGGCTACCTACCGTTCAGCGCCTCTGGAGGCGCCTTGCTGTTCCATCTGCTGTCAAAG
CTGTACGAGCGCACGAGCGTCGTGATCACCACCAACCTGAGCTTCAGCGAATGGGCCAGTGTGTTCGGGGATGCAAAGATGACCACTGCATTGCTGGACC
GGCTCACGCATCACTGCCATATTCTGGAAACCGGCAACGACAGCTACAGGTTCAAGAACAGCTCCGCACAGCAACCACCACACACCACCAAGAAGGAGAA
GGCGACCAAGAACTTATCCACAACGTGAGCGTGAAGTTCACGAACAAGGGTGGGTCAAGATTCGATGGAAATGGTGGGTCAGGTTTGCATGGAAATCAAC
A
GACCAGGAGTGATAGACGTGGCAACACTGAGTGTCATCAGGCGCTGGGCCCTGCGAGAGCAGCTGTCCATCAGGGAGATCGCCCGCCGCACGGGCGTCTC
GCGCAACACCATCCGCAAGTACCTGCGAGCAGGCGAGGCCGAGCCGCGCTACCCCAAGCGGGTCAGCCGTTCCGGGCTCGATCCCTTTGCCTTGAAGCTC
TCGGGCTGGCTCAAGACAGAAGCAGGCCGATCCCGTAAGCAGCGGCGCACCGTCAAGCAGATGCACGCCGATCTGCAGGCCCTGGGCTATACCGGCTCCT
ACAACCGGGTTGCCGCCTTTGCCCGTCTCTGGCAAGAGCAGCGCGTGGTGGCCCAGCAGACCACCGGCCGCGGCACCTTCGTTCCCCTGGCCTTCGGCCC
GGGCGAGGCCTTCCAGTTCGACTGGAGCGAAGACTGGGCAGTGCTGGCCGGAGTGCGCACCAAGCTGCAGGTAGCCCACTTCAAGCTCAGCCACAGCCGA
GCGTTCTACCTGCGCGCCTATCCGCTGCAGACGCACGAGATGCTGTTCGATGCCCACAACCATGCATTCGCAGTCCTGGGCGGTGTGCCCCGCCGTGGCA
TCTACGACAACATGCGCACCGCTGTAGACCGGGTGCGCCGTGGCAAGGAACGCGACGTCAATGCGCGCTTTGCGGCCATGGTCAGTCACTTCCTGTTCGA
GGCCGAGTTCTGCAACCCTGCCTCCGGCTGGGAGAAGGGCCAGGTGGAGAAGAATGTGCGCGATGCCCGTCATCGCCTGTGGCAGGTCGTTCCGCCATTC
CGTGGCCTGACGGAGCTCAATGCCTGGCTTGAGGAGCGCTGCGTGGCCTTATGGAATGAGATCGAGCATGGCAAGCTGCCGGGCACCGTCGCGGACGTCT
GGGCCGCGGAGCGGGCTGCCTTGATGCCGGTGCCCAGGACCTTCGATGGCTTTGTGGAGCACACAAAGCGGGTCTCGCCCACCTGCCTGGTCCACTTCGA
GCGCAATCGCTACAGCGTACCAGCGCCGTATGCCAATCGGCCGGTGAGCCTGCGGGTCTACGCGGATCGCCTAGTGGTCGCGGCCGAAGGCCAGATCGTC
TGCGAGCACCGGCGCCTGATCGAACGCAACCACCATGGTGCGGGACAGACCGTCTACGACTGGCGCCACTACCTGGCGGTGTTGCAGCGCAAGCCCGGGG
CCTTGCGCAATGGCGCGCCGTTCCTGGAGTTGCCGCCCGCCTTCAAGCGCCTGCAGGCCACCTTGCTCAAGCAACCCGGCGGCGACCGGGAGATGGTGGA
GGTTCTGGCACTGGTGCTGCACCACGATGAACAAGCTGTGCTCGCCGCAGTGGAGCTGGCCATAGAGTCGGGAGCTGCCAGCAAGACCCACATCCTGAAC
GTGCTCCACCGCCTGCTGGACGGCAAGCCCGCTCCAGCACCGGTCACCTCGCCCCAGGCTCTCAAGCTGGCGGTGGAACCCCAGGCCAATGTGCTTCGCT
ACGACCAATTGAGGGAGGTGCGCTATGCGTCATGACCCTGCCATCGCTTCCATCGTGATCATGCTGCGCGAGCTCAAGATGCACGGCATGGCCCAGGCGG
TTGCGGAACTGGCCGAGCAAGGTGCGCCGGCCTTCGACGCAGCACAGCCCATCCTGTCCCAACTGCTCAAGGCCGAGACCGCCGAGCGGGAGGTGCGCTC
AGTGGCTTACCAATTGAAGGTGGCCAGGTTCCCTGCGTACCGGGACATGGCGGGCTTTGACTTCAGCCTCAGCGAGGTGAACGAGGCACTGGTGCGCCAG
CTGCACCGCTGCGAATTCTTGGAGAACGCCAACAACGTGGTGCTGGTGGGTGGACCGGGTACGGGCAAGACCCACATTGCCACAGCCCTCGGAGTGCAAG
CCATTGAGCACCATCACCGCAGGGTGCGGTTCTTCTCCACGGTGGAGCTGGTCAATGCGTTGGAGGAGGAGAAGGCCAACGGCAAGCCCGGGCAGTTGGC
CCACCGCCTGGCCTATGCCGATCTGGTGATCCTGGATGAACTGGGCTACCTACCGTTCAGCGCCTCTGGAGGCGCCTTGCTGTTCCATCTGCTGTCAAAG
CTGTACGAGCGCACGAGCGTCGTGATCACCACCAACCTGAGCTTCAGCGAATGGGCCAGTGTGTTCGGGGATGCAAAGATGACCACTGCATTGCTGGACC
GGCTCACGCATCACTGCCATATTCTGGAAACCGGCAACGACAGCTACAGGTTCAAGAACAGCTCCGCACAGCAACCACCACACACCACCAAGAAGGAGAA
GGCGACCAAGAACTTATCCACAACGTGAGCGTGAAGTTCACGAACAAGGGTGGGTCAAGATTCGATGGAAATGGTGGGTCAGGTTTGCATGGAAATCAAC
A
Protein section
ORF number : 2
ORF 1
Length | Begin | End | Strand | Fusion ORF | |
---|---|---|---|---|---|
1527 bp | 508 aa | 109 | 1635 | + | No |
Chemistry : DDE
ORF sequence :
MIDVATLSVIRRWALREQLSIREIARRTGVSRNTIRKYLRAGEAEPRYPKRVSRSGLDPFALKLSGWLKTEAGRSRKQRRTVKQMHADLQALGYTGSYNR
VAAFARLWQEQRVVAQQTTGRGTFVPLAFGPGEAFQFDWSEDWAVLAGVRTKLQVAHFKLSHSRAFYLRAYPLQTHEMLFDAHNHAFAVLGGVPRRGIYD
NMRTAVDRVRRGKERDVNARFAAMVSHFLFEAEFCNPASGWEKGQVEKNVRDARHRLWQVVPPFRGLTELNAWLEERCVALWNEIEHGKLPGTVADVWAA
ERAALMPVPRTFDGFVEHTKRVSPTCLVHFERNRYSVPAPYANRPVSLRVYADRLVVAAEGQIVCEHRRLIERNHHGAGQTVYDWRHYLAVLQRKPGALR
NGAPFLELPPAFKRLQATLLKQPGGDREMVEVLALVLHHDEQAVLAAVELAIESGAASKTHILNVLHRLLDGKPAPAPVTSPQALKLAVEPQANVLRYDQ
LREVRYAS
VAAFARLWQEQRVVAQQTTGRGTFVPLAFGPGEAFQFDWSEDWAVLAGVRTKLQVAHFKLSHSRAFYLRAYPLQTHEMLFDAHNHAFAVLGGVPRRGIYD
NMRTAVDRVRRGKERDVNARFAAMVSHFLFEAEFCNPASGWEKGQVEKNVRDARHRLWQVVPPFRGLTELNAWLEERCVALWNEIEHGKLPGTVADVWAA
ERAALMPVPRTFDGFVEHTKRVSPTCLVHFERNRYSVPAPYANRPVSLRVYADRLVVAAEGQIVCEHRRLIERNHHGAGQTVYDWRHYLAVLQRKPGALR
NGAPFLELPPAFKRLQATLLKQPGGDREMVEVLALVLHHDEQAVLAAVELAIESGAASKTHILNVLHRLLDGKPAPAPVTSPQALKLAVEPQANVLRYDQ
LREVRYAS
Blast result :ORF 2
Length | Begin | End | Strand | Fusion ORF | |
---|---|---|---|---|---|
804 bp | 267 aa | 1625 | 2428 | + | No |
AG : IS21 helper
ORF sequence :
MRHDPAIASIVIMLRELKMHGMAQAVAELAEQGAPAFDAAQPILSQLLKAETAEREVRSVAYQLKVARFPAYRDMAGFDFSLSEVNEALVRQLHRCEFLE
NANNVVLVGGPGTGKTHIATALGVQAIEHHHRRVRFFSTVELVNALEEEKANGKPGQLAHRLAYADLVILDELGYLPFSASGGALLFHLLSKLYERTSVV
ITTNLSFSEWASVFGDAKMTTALLDRLTHHCHILETGNDSYRFKNSSAQQPPHTTKKEKATKNLSTT
NANNVVLVGGPGTGKTHIATALGVQAIEHHHRRVRFFSTVELVNALEEEKANGKPGQLAHRLAYADLVILDELGYLPFSASGGALLFHLLSKLYERTSVV
ITTNLSFSEWASVFGDAKMTTALLDRLTHHCHILETGNDSYRFKNSSAQQPPHTTKKEKATKNLSTT
Blast result :
Comments
ISAlsp is 94% (transposase) and 96% (IS21 helper) aa similar to ISThsp10.
References
1] Thomas Jové (2016) Direct submission.
2] Mampel J, Ruff J, Junker F, Cook AM. Microbiology. (1999) Nov;145 (Pt 11):3255-64.
2] Mampel J, Ruff J, Junker F, Cook AM. Microbiology. (1999) Nov;145 (Pt 11):3255-64.