ISAlsp1

  • Family IS21
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
AF109074 ND Alcaligenes sp.
Alcaligenes sp. O-1
DNA section
IS Length : 2501 bp

Ends


IR Length : 24/28

IRL : tgttgatttccacgcagaagtgacccactgacccccaggatttccatcta
IRR : tgttgatttccatgcaaacctgacccaccatttccatcgaatcttgaccc

Insertion site


Left flankDirect repeatRight flankDR Length
gggcacggatttgagggcca0

DNA sequence

TGTTGATTTCCACGCAGAAGTGACCCACTGACCCCCAGGATTTCCATCTAAACCTGACCCACGTACTAACCCTAACCTGCTGCTTCTTTGAGCAGCAGGA
GACCAGGAGTGATAGACGTGGCAACACTGAGTGTCATCAGGCGCTGGGCCCTGCGAGAGCAGCTGTCCATCAGGGAGATCGCCCGCCGCACGGGCGTCTC
GCGCAACACCATCCGCAAGTACCTGCGAGCAGGCGAGGCCGAGCCGCGCTACCCCAAGCGGGTCAGCCGTTCCGGGCTCGATCCCTTTGCCTTGAAGCTC
TCGGGCTGGCTCAAGACAGAAGCAGGCCGATCCCGTAAGCAGCGGCGCACCGTCAAGCAGATGCACGCCGATCTGCAGGCCCTGGGCTATACCGGCTCCT
ACAACCGGGTTGCCGCCTTTGCCCGTCTCTGGCAAGAGCAGCGCGTGGTGGCCCAGCAGACCACCGGCCGCGGCACCTTCGTTCCCCTGGCCTTCGGCCC
GGGCGAGGCCTTCCAGTTCGACTGGAGCGAAGACTGGGCAGTGCTGGCCGGAGTGCGCACCAAGCTGCAGGTAGCCCACTTCAAGCTCAGCCACAGCCGA
GCGTTCTACCTGCGCGCCTATCCGCTGCAGACGCACGAGATGCTGTTCGATGCCCACAACCATGCATTCGCAGTCCTGGGCGGTGTGCCCCGCCGTGGCA
TCTACGACAACATGCGCACCGCTGTAGACCGGGTGCGCCGTGGCAAGGAACGCGACGTCAATGCGCGCTTTGCGGCCATGGTCAGTCACTTCCTGTTCGA
GGCCGAGTTCTGCAACCCTGCCTCCGGCTGGGAGAAGGGCCAGGTGGAGAAGAATGTGCGCGATGCCCGTCATCGCCTGTGGCAGGTCGTTCCGCCATTC
CGTGGCCTGACGGAGCTCAATGCCTGGCTTGAGGAGCGCTGCGTGGCCTTATGGAATGAGATCGAGCATGGCAAGCTGCCGGGCACCGTCGCGGACGTCT
GGGCCGCGGAGCGGGCTGCCTTGATGCCGGTGCCCAGGACCTTCGATGGCTTTGTGGAGCACACAAAGCGGGTCTCGCCCACCTGCCTGGTCCACTTCGA
GCGCAATCGCTACAGCGTACCAGCGCCGTATGCCAATCGGCCGGTGAGCCTGCGGGTCTACGCGGATCGCCTAGTGGTCGCGGCCGAAGGCCAGATCGTC
TGCGAGCACCGGCGCCTGATCGAACGCAACCACCATGGTGCGGGACAGACCGTCTACGACTGGCGCCACTACCTGGCGGTGTTGCAGCGCAAGCCCGGGG
CCTTGCGCAATGGCGCGCCGTTCCTGGAGTTGCCGCCCGCCTTCAAGCGCCTGCAGGCCACCTTGCTCAAGCAACCCGGCGGCGACCGGGAGATGGTGGA
GGTTCTGGCACTGGTGCTGCACCACGATGAACAAGCTGTGCTCGCCGCAGTGGAGCTGGCCATAGAGTCGGGAGCTGCCAGCAAGACCCACATCCTGAAC
GTGCTCCACCGCCTGCTGGACGGCAAGCCCGCTCCAGCACCGGTCACCTCGCCCCAGGCTCTCAAGCTGGCGGTGGAACCCCAGGCCAATGTGCTTCGCT
ACGACCAATTGAGGGAGGTGCGCTATGCGTCATGACCCTGCCATCGCTTCCATCGTGATCATGCTGCGCGAGCTCAAGATGCACGGCATGGCCCAGGCGG
TTGCGGAACTGGCCGAGCAAGGTGCGCCGGCCTTCGACGCAGCACAGCCCATCCTGTCCCAACTGCTCAAGGCCGAGACCGCCGAGCGGGAGGTGCGCTC
AGTGGCTTACCAATTGAAGGTGGCCAGGTTCCCTGCGTACCGGGACATGGCGGGCTTTGACTTCAGCCTCAGCGAGGTGAACGAGGCACTGGTGCGCCAG
CTGCACCGCTGCGAATTCTTGGAGAACGCCAACAACGTGGTGCTGGTGGGTGGACCGGGTACGGGCAAGACCCACATTGCCACAGCCCTCGGAGTGCAAG
CCATTGAGCACCATCACCGCAGGGTGCGGTTCTTCTCCACGGTGGAGCTGGTCAATGCGTTGGAGGAGGAGAAGGCCAACGGCAAGCCCGGGCAGTTGGC
CCACCGCCTGGCCTATGCCGATCTGGTGATCCTGGATGAACTGGGCTACCTACCGTTCAGCGCCTCTGGAGGCGCCTTGCTGTTCCATCTGCTGTCAAAG
CTGTACGAGCGCACGAGCGTCGTGATCACCACCAACCTGAGCTTCAGCGAATGGGCCAGTGTGTTCGGGGATGCAAAGATGACCACTGCATTGCTGGACC
GGCTCACGCATCACTGCCATATTCTGGAAACCGGCAACGACAGCTACAGGTTCAAGAACAGCTCCGCACAGCAACCACCACACACCACCAAGAAGGAGAA
GGCGACCAAGAACTTATCCACAACGTGAGCGTGAAGTTCACGAACAAGGGTGGGTCAAGATTCGATGGAAATGGTGGGTCAGGTTTGCATGGAAATCAAC
A
Protein section
ORF number : 2

 

ORF 1
LengthBeginEndStrandFusion ORF
1527 bp508 aa1091635+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MIDVATLSVIRRWALREQLSIREIARRTGVSRNTIRKYLRAGEAEPRYPKRVSRSGLDPFALKLSGWLKTEAGRSRKQRRTVKQMHADLQALGYTGSYNR
VAAFARLWQEQRVVAQQTTGRGTFVPLAFGPGEAFQFDWSEDWAVLAGVRTKLQVAHFKLSHSRAFYLRAYPLQTHEMLFDAHNHAFAVLGGVPRRGIYD
NMRTAVDRVRRGKERDVNARFAAMVSHFLFEAEFCNPASGWEKGQVEKNVRDARHRLWQVVPPFRGLTELNAWLEERCVALWNEIEHGKLPGTVADVWAA
ERAALMPVPRTFDGFVEHTKRVSPTCLVHFERNRYSVPAPYANRPVSLRVYADRLVVAAEGQIVCEHRRLIERNHHGAGQTVYDWRHYLAVLQRKPGALR
NGAPFLELPPAFKRLQATLLKQPGGDREMVEVLALVLHHDEQAVLAAVELAIESGAASKTHILNVLHRLLDGKPAPAPVTSPQALKLAVEPQANVLRYDQ
LREVRYAS

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
804 bp267 aa16252428+No
ORF function : Accessory Gene
AG : IS21 helper

ORF sequence :

MRHDPAIASIVIMLRELKMHGMAQAVAELAEQGAPAFDAAQPILSQLLKAETAEREVRSVAYQLKVARFPAYRDMAGFDFSLSEVNEALVRQLHRCEFLE
NANNVVLVGGPGTGKTHIATALGVQAIEHHHRRVRFFSTVELVNALEEEKANGKPGQLAHRLAYADLVILDELGYLPFSASGGALLFHLLSKLYERTSVV
ITTNLSFSEWASVFGDAKMTTALLDRLTHHCHILETGNDSYRFKNSSAQQPPHTTKKEKATKNLSTT

 

Blast result :
Comments
ISAlsp is 94% (transposase) and 96% (IS21 helper) aa similar to ISThsp10.
References
1] Thomas Jové (2016) Direct submission.
2] Mampel J, Ruff J, Junker F, Cook AM. Microbiology. (1999) Nov;145 (Pt 11):3255-64.