ISAlde2
- Family IS21
- Group
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| CP002449 | ND | Alicycliphilus denitrificans | Alicycliphilus denitrificans BC Alicycliphilus denitrificans BC plasmid pALIDE01 |
DNA section
IS Length : 2501 bp
Ends
IR Length : 24/28
IRL : TGTTGATTtccacgcagaagtgacccactaacccccaggatttccatcta
IRR : TGTTGATTTccatgcaaagctgacccaccatttccatcgaatcttgaccc
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| aacccataga | gcactggccg | 0 | |
| acagccacac | accttgccgt | 0 | |
| gacgcctggg | ccgtgctttc | 0 | |
| TTTTCATGGA | CACCGGCTAA | 0 |
DNA sequence
TGTTGATTTCCACGCAGAAGTGACCCACTAACCCCCAGGATTTCCATCTAAATCTGACCCACGTACTAACCCTAACCTGCTGCTTTGTTGAGCAGCAGGA
GACCAGGAGTGATAGACGTGGCAACACTGAGTGTCATCAGGCGCTGGGCCCTGCGAGAGCAGCTGTCCATCCGAGAGATCGCCCGCCGCACGGGCCTCTC
GCGCAACACCATCCGCAAGTACCTGCGCGCAGGCGAGGCCGAGCCGCACTATGCCAAGCGGGTCAGCCCTTCCAAGCTCGATCCCTTTGCCTTGAAGCTC
TCGGGCTGGCTCAAGACGGAAGCTGGCCGATCCCGTAAGCAGCGGCGTACCGTCAAGCAGATATACGCCGATCTGCAGGCGCTGGGCTATACCGGCTCCT
ACAACCGGGTCGCGGCCTTTGTCCGCCTCTGGCAAGAACAACGCCTGGTGGCCCAGCAGACCACCGGCCGCGGCACCTTTGTGCCTTTGGCCTTCGGCCC
CGGCGAGGCGTTCCAATTCGATTGGAGCGAGGACTGGGCAGTGCTCGCAGGTGTGCGCACCAAGCTGCAGGTGGCTCACTTCAAACTCAGTCACAGCCGG
GCGTTCTACCTGCGTGCCTATCTTCTGCAGACGCACGAGATGCTGTTCGATGCCCACAACCATGCGTTCGTCGTGCTGGGCGGTGTGCCCCGCCGAGGCA
TCTACGACAACATGCGCACCGCCATAGACCGCGTGCGCCGGGGCAAGGAGAGAGATGTCAACGCACGCTTTGCTGCCATGGTCAGCCACTTCCTGTTCGA
GGCAGAGTTCTGCAACCCGGCCTCTGGCTGGGAGAAGGGCCAGGTGGAGAAGAACGTGCGCGATGCCCGCCACCGCCTGTGGCAGGTGGTGCCGCCATTC
CCGAGCCTGCCCGATCTGAATGCCTGGCTTGAGGAGCGCTGTGTGGCCCTGTGGCACGAGATCGAGCATGGCAAGCTGGCTGGCACCGTCGCGGACGTCT
GGGCCGAGGAGCGGGCAGCCTTGATGCCTGTGCCCCGGCCCTTCGATGGCTTTGTGGAGCACACCAAGCGCGTCTCACCCACCTGCTTGGTCCACTTCGA
GCGCAATCGCTACAGCGTGCCAGCGCCGTATGCCAACCGGCCTGTGAGCCTGCGGGTCTACGCGGACCGCCTGGTGGTCGCTGCCGAAGGCCAGATCATC
TGTGAACACCAGCGCCTGATCGAGCGCAACCACCATGGCGCTGGGCAGACCGTCTATGACTGGCGCCACTACCTGGCGGTGCTGCAGCGCAAGCCCGGGG
CATTGCGCAATGGCGCTCCCTTCCAGGAACTGCCAGTGGCCTTCAAACGGCTGCAGACCGCCCTCTTGATGCAGCCAGGCGGCGACAGAGAGATGGTGGA
GGTGCTGGCACTGGTGCTGCATCACGACGAGCAGGCCGTGCTCGCCGCAGTGGAACTGGCCCTGGAGTCTGGGGCTGCCAGCAAGACCCATATCCTGAAC
GTGCTGCACCGGCTGCTGGACGGCAAGCCCGCTCCAGCACCGGTCACCTCCCCCCAGGCCCTCAAGCTGGCGATGGAACCCCAGGCCAACGTGCTGCGCT
ATGACCAATTGAGGGAGGTGCGCTATGCGTCATGACCCCGCCATCGCTTCCATCGTGATCATGCTGCGCGAGCTCAAGATGCACGGCATGGCCCAGGCAG
TTGCGGAACTGGCTGAGCAAGGTGCGCCAGCCTTCGAGGCAGCACAGCCCATCCTGTCCCAACTGCTCAAGGCTGAGACCGCCGAGCGGGAGGTGCGCTC
GGTGGCTTACCAATTGAAGGTGGCCAGGTTCCCCGCGTACCGGGACCTGGCAGGCTTCGACTTCAGCCACAGCGAGGTGAACGAAGCCCTGGTGCGCCAG
TTGCACCGGTGTGAGTTCTTGGAGGATGCCAACAACGTGGTGCTGGTGGGCGGGCCGGGCACAGGCAAGACCCACATCGCCACAGCCCTCGGAGTGCAAG
CCATTGAACACCATCACCGAAGGGTGCGGTTCTTCTCCACGGTAGAGTTGGTCAATGCGCTGGAAGAAGAGAAGGCCCAAGGCAAGACAGGGCAGATGGC
CCACCGGCTTGCATATGTGGATCTGGTGATTCTGGACGAGTTGGGCTACCTGCCGTTCAGTGCATCAGGAGGCGCCTTGCTGTTCCACCTGCTGTCCAAA
CTCTACGAGCGCACCAGCGTCGTGATCACCACCAACCTGAGCTTCAGCGAGTGGGCCAGTGTGTTCGGGGATGCCAAGATGACCACGGCATTGCTCGACC
GGCTCACGCATCACTGCCATATTCTTGAGACCGGCAATGACAGCTACCGGTTCAAGAATAGCTCCGCACAGCAACCACCACAGACCACCAAGAAGGAGAA
GGCGACCAAGAACTTATCCACAACGTGAGCGTGAAGTTCACGAATCAGGGTGGGTCAAGATTCGATGGAAATGGTGGGTCAGCTTTGCATGGAAATCAAC
A
GACCAGGAGTGATAGACGTGGCAACACTGAGTGTCATCAGGCGCTGGGCCCTGCGAGAGCAGCTGTCCATCCGAGAGATCGCCCGCCGCACGGGCCTCTC
GCGCAACACCATCCGCAAGTACCTGCGCGCAGGCGAGGCCGAGCCGCACTATGCCAAGCGGGTCAGCCCTTCCAAGCTCGATCCCTTTGCCTTGAAGCTC
TCGGGCTGGCTCAAGACGGAAGCTGGCCGATCCCGTAAGCAGCGGCGTACCGTCAAGCAGATATACGCCGATCTGCAGGCGCTGGGCTATACCGGCTCCT
ACAACCGGGTCGCGGCCTTTGTCCGCCTCTGGCAAGAACAACGCCTGGTGGCCCAGCAGACCACCGGCCGCGGCACCTTTGTGCCTTTGGCCTTCGGCCC
CGGCGAGGCGTTCCAATTCGATTGGAGCGAGGACTGGGCAGTGCTCGCAGGTGTGCGCACCAAGCTGCAGGTGGCTCACTTCAAACTCAGTCACAGCCGG
GCGTTCTACCTGCGTGCCTATCTTCTGCAGACGCACGAGATGCTGTTCGATGCCCACAACCATGCGTTCGTCGTGCTGGGCGGTGTGCCCCGCCGAGGCA
TCTACGACAACATGCGCACCGCCATAGACCGCGTGCGCCGGGGCAAGGAGAGAGATGTCAACGCACGCTTTGCTGCCATGGTCAGCCACTTCCTGTTCGA
GGCAGAGTTCTGCAACCCGGCCTCTGGCTGGGAGAAGGGCCAGGTGGAGAAGAACGTGCGCGATGCCCGCCACCGCCTGTGGCAGGTGGTGCCGCCATTC
CCGAGCCTGCCCGATCTGAATGCCTGGCTTGAGGAGCGCTGTGTGGCCCTGTGGCACGAGATCGAGCATGGCAAGCTGGCTGGCACCGTCGCGGACGTCT
GGGCCGAGGAGCGGGCAGCCTTGATGCCTGTGCCCCGGCCCTTCGATGGCTTTGTGGAGCACACCAAGCGCGTCTCACCCACCTGCTTGGTCCACTTCGA
GCGCAATCGCTACAGCGTGCCAGCGCCGTATGCCAACCGGCCTGTGAGCCTGCGGGTCTACGCGGACCGCCTGGTGGTCGCTGCCGAAGGCCAGATCATC
TGTGAACACCAGCGCCTGATCGAGCGCAACCACCATGGCGCTGGGCAGACCGTCTATGACTGGCGCCACTACCTGGCGGTGCTGCAGCGCAAGCCCGGGG
CATTGCGCAATGGCGCTCCCTTCCAGGAACTGCCAGTGGCCTTCAAACGGCTGCAGACCGCCCTCTTGATGCAGCCAGGCGGCGACAGAGAGATGGTGGA
GGTGCTGGCACTGGTGCTGCATCACGACGAGCAGGCCGTGCTCGCCGCAGTGGAACTGGCCCTGGAGTCTGGGGCTGCCAGCAAGACCCATATCCTGAAC
GTGCTGCACCGGCTGCTGGACGGCAAGCCCGCTCCAGCACCGGTCACCTCCCCCCAGGCCCTCAAGCTGGCGATGGAACCCCAGGCCAACGTGCTGCGCT
ATGACCAATTGAGGGAGGTGCGCTATGCGTCATGACCCCGCCATCGCTTCCATCGTGATCATGCTGCGCGAGCTCAAGATGCACGGCATGGCCCAGGCAG
TTGCGGAACTGGCTGAGCAAGGTGCGCCAGCCTTCGAGGCAGCACAGCCCATCCTGTCCCAACTGCTCAAGGCTGAGACCGCCGAGCGGGAGGTGCGCTC
GGTGGCTTACCAATTGAAGGTGGCCAGGTTCCCCGCGTACCGGGACCTGGCAGGCTTCGACTTCAGCCACAGCGAGGTGAACGAAGCCCTGGTGCGCCAG
TTGCACCGGTGTGAGTTCTTGGAGGATGCCAACAACGTGGTGCTGGTGGGCGGGCCGGGCACAGGCAAGACCCACATCGCCACAGCCCTCGGAGTGCAAG
CCATTGAACACCATCACCGAAGGGTGCGGTTCTTCTCCACGGTAGAGTTGGTCAATGCGCTGGAAGAAGAGAAGGCCCAAGGCAAGACAGGGCAGATGGC
CCACCGGCTTGCATATGTGGATCTGGTGATTCTGGACGAGTTGGGCTACCTGCCGTTCAGTGCATCAGGAGGCGCCTTGCTGTTCCACCTGCTGTCCAAA
CTCTACGAGCGCACCAGCGTCGTGATCACCACCAACCTGAGCTTCAGCGAGTGGGCCAGTGTGTTCGGGGATGCCAAGATGACCACGGCATTGCTCGACC
GGCTCACGCATCACTGCCATATTCTTGAGACCGGCAATGACAGCTACCGGTTCAAGAATAGCTCCGCACAGCAACCACCACAGACCACCAAGAAGGAGAA
GGCGACCAAGAACTTATCCACAACGTGAGCGTGAAGTTCACGAATCAGGGTGGGTCAAGATTCGATGGAAATGGTGGGTCAGCTTTGCATGGAAATCAAC
A
Protein section
ORF number : 2
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1527 bp | 508 aa | 109 | 1635 | + | No |
Chemistry : DDE
ORF sequence :
MIDVATLSVIRRWALREQLSIREIARRTGLSRNTIRKYLRAGEAEPHYAKRVSPSKLDPFALKLSGWLKTEAGRSRKQRRTVKQIYADLQALGYTGSYNR
VAAFVRLWQEQRLVAQQTTGRGTFVPLAFGPGEAFQFDWSEDWAVLAGVRTKLQVAHFKLSHSRAFYLRAYLLQTHEMLFDAHNHAFVVLGGVPRRGIYD
NMRTAIDRVRRGKERDVNARFAAMVSHFLFEAEFCNPASGWEKGQVEKNVRDARHRLWQVVPPFPSLPDLNAWLEERCVALWHEIEHGKLAGTVADVWAE
ERAALMPVPRPFDGFVEHTKRVSPTCLVHFERNRYSVPAPYANRPVSLRVYADRLVVAAEGQIICEHQRLIERNHHGAGQTVYDWRHYLAVLQRKPGALR
NGAPFQELPVAFKRLQTALLMQPGGDREMVEVLALVLHHDEQAVLAAVELALESGAASKTHILNVLHRLLDGKPAPAPVTSPQALKLAMEPQANVLRYDQ
LREVRYAS*
VAAFVRLWQEQRLVAQQTTGRGTFVPLAFGPGEAFQFDWSEDWAVLAGVRTKLQVAHFKLSHSRAFYLRAYLLQTHEMLFDAHNHAFVVLGGVPRRGIYD
NMRTAIDRVRRGKERDVNARFAAMVSHFLFEAEFCNPASGWEKGQVEKNVRDARHRLWQVVPPFPSLPDLNAWLEERCVALWHEIEHGKLAGTVADVWAE
ERAALMPVPRPFDGFVEHTKRVSPTCLVHFERNRYSVPAPYANRPVSLRVYADRLVVAAEGQIICEHQRLIERNHHGAGQTVYDWRHYLAVLQRKPGALR
NGAPFQELPVAFKRLQTALLMQPGGDREMVEVLALVLHHDEQAVLAAVELALESGAASKTHILNVLHRLLDGKPAPAPVTSPQALKLAMEPQANVLRYDQ
LREVRYAS*
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 804 bp | 255 aa | 1625 | 2428 | + | No |
AG : IS21 helper
ORF sequence :
MRHDPAIASIVIMLRELKMHGMAQAVAELAEQGAPAFEAAQPILSQLLKAETAEREVRSVAYQLKVARFPAYRDLAGFDFSHSEVNEALVRQLHRCEFLE
DANNVVLVGGPGTGKTHIATALGVQAIEHHHRRVRFFSTVELVNALEEEKAQGKTGQMAHRLAYVDLVILDELGYLPFSASGGALLFHLLSKLYERTSVV
ITTNLSFSEWASVFGDAKMTTALLDRLTHHCHILETGNDSYRFKNSSAQQPPQTTKKEKATKNLSTT
DANNVVLVGGPGTGKTHIATALGVQAIEHHHRRVRFFSTVELVNALEEEKAQGKTGQMAHRLAYVDLVILDELGYLPFSASGGALLFHLLSKLYERTSVV
ITTNLSFSEWASVFGDAKMTTALLDRLTHHCHILETGNDSYRFKNSSAQQPPQTTKKEKATKNLSTT
Blast result :
Comments
ISAlde2 is 97% (transposase) and 99% (helper) aa similar to ISAlde1.
References
1] Thomas Jové (2016) Direct submission.
2] Oosterkamp MJ , Veuskens T, Plugge CM, Langenhoff AA, Gerritse J, van Berkel WJ, Pieper DH, Junca H, Goodwin LA, Daligault HE, Bruce DC, Detter JC, Tapia R, Han CS, Land ML, Hauser LJ, Smidt H, Stams AJ. (2011) J Bacteriol. Sep;193(18):5028-9.
2] Oosterkamp MJ , Veuskens T, Plugge CM, Langenhoff AA, Gerritse J, van Berkel WJ, Pieper DH, Junca H, Goodwin LA, Daligault HE, Bruce DC, Detter JC, Tapia R, Han CS, Land ML, Hauser LJ, Smidt H, Stams AJ. (2011) J Bacteriol. Sep;193(18):5028-9.