ISPa65

  • Family IS21
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
LC075717 ND Pseudomonas aeruginosa
Pseudomonas aeruginosa NCGM 2991
DNA section
IS Length : 2520 bp

Ends


IR Length : 27

IRL : tgttgaatcacgagcaaaactgagccactgaagggtctccgtcacgtcca
IRR : tgttgaatcacgagcaaaactgagccaggcgtcacgtccaaaactgagcc

Insertion site


Left flankDirect repeatRight flankDR Length
gcaggaccatcgtagttttc0

DNA sequence

TGTTGAATCACGAGCAAAACTGAGCCACTGAAGGGTCTCCGTCACGTCCAATTTTGAGCCACGTTATCCACCTATCCTGCTGCTTTTTGCAGCAGCGGGG
GACAGGAGTGATCAACGTGAGCACATTGAGCAAATTGCGCCGCATGGTCCACCGTGACCACCTGAGCGTGCGCGAAGCCAGCCGCAGGCTTGGCATCTCC
CGCAACACGGCCACCAAGTGGCTTGAAGCCGATGAGATGGTCGAACCCCGTTATCCCAAGCGGGCGGCCATCCCGAGCGTTCTAGATCCTTACAAGGAAC
AGCTGTCCACCTGGCTGAAGGCCGACAGCCACCGCAACAAGCGCGAGCGCCGTGGCATCAAGGCCATGTTCCGGGCATTGCGGGCCATGGGCTATCCGGG
CAGCCGAGGCCCGGTCTATGAGTTCGCCAAGCGCTGGCAGCAGGCGCAGTCCGATGCGCCCACGCGCATGGCCTTCGTGCCCATGAGTTTCGAGATGGGC
GAGGCCTTCCAGTTCGACTGGAGCTGCGAGTACCTGTTCGTGGGCGGCATGCGCAAACGCCTGGAGGCCGCGCACACCAAGCTGGCGGCCAGCCGCGCCT
TCATGCTCACCGCCTACTTCAGCCAGGCCCACGAGATGCTGTTTGATGCCCACGCGCGGGCCTTTGCTGCCTTCGGTGGCGTGCCCCGCCGGGGCATCTA
CGACAACATGAAGACCGCTGTCGACAAGGTTGGCCAGGGCAAGAGCCGCACGGTCAACGCCCGCTTCGAGGCCATGACGGGCCACTACCTGTTCGAGCCT
GAGTTCTGCAACCGCGCCGCCGGCTGGGAGAAGGGCATTGTCGAGAAGAACGTGCAGGATCGCCGTCGCGGCATCTGGCTGGAGGCGGCCGAACGCCGCT
GGCCTGACCTGGAGAGCCTCAATGCGTGGCTGCACCAGGCCTGCCTGGACGCCTGGCATGAACTGCCGCACCCCGAGTGGCCCGAGCTGACCATTGCCGA
CGTCTGGCAGCAAGAGCAGACGCACCTGATGCCCAACCCTGCGCCATTCGATGGCTATGTCGAGCAGGTGGCACGGGTAACGGCCACCTCGCTGATCCAC
TATCAGCGCAACCGCTACAGCGTGCCTTGCGAGTGGGCGCACGCAACGGTGAGCCTGCGCGCCTATCCTGACCGCTTGGTGGTGGTCGGCCCCAGCACCG
ATGCGCCCGAGCCGCCAGTGAGCCTGCCACGCAGTTTTGAGCGTGGGCAGACCTTGTACGACTGGCGCCATTACATCAGCCTGCTCGAACGCAAGCCCGG
GGCCTTGCGCAACGGCGCGCCCTTCAAGACCATGCCCGAGCCCCTGCAACATCTTCAGGCGCACCTGCTGCGCCACCCTGGCGGCGACCGCGTGATGGCC
CAGGTGCTCATGGCGATTACCTTGCACGGGCTCGATGACGTGCTCGTTGCCGTCGAGTTGGCGCTGCAATCCGGCCGCGTGAGCGCCGACCATGTGCTCA
ACGTACTGGCCAGGCTCAAGGAACCCGAAGCTGTGCACAGCTTGCCCGAAGCCGCCTTGACTTCACTGACGTTGCAGGAGCCGCCTCAGGCCGATGTGTC
GCGCTATGACCGCCTGCGCCAGATCCAGGAGGGTGACCATGTCCAATGACATCGCCGCTGGCCTCAAGGGCTTGAGCCTGCACGGCATGGCCAGTGCCTG
GCCTGAACTGCTGGGTATCGCGCGGCTCAAGTCGCTCGACCATGAAGCCTTGCTGCATCAGCTCATCAAGGCCGAGGGAGCGCACCGGGAAGTGCGCTCC
ATGGCTTACCAGATGCGGGTGGCCCGATTCCCACACCACCGCGACCTGGCAGGCTTTGCATTTGATCAGGCCCAGGTCGACGAGGCACTGGTGCGTCAGT
TGCACGAACTCAGGTTCATCGACTCAGCCCACAACGTGGTCTTCGTGGGCGGCCCTGGCACCGGCAAGACACACCTAGCCACCAGCCTCGGCGTTCATGC
CATCCGTGCACATGGCAAGCGGGTGCGCTTCTTCTCGACCGTAGAACTGGTCAACATGCTGGAAGCAGAGAAGGCGCAGGGCAAGGCCGGGCAACTGGCG
CATCGGCTCATGTACGTCGATCTCGTCATCCTCGACGAGATGGGCTACCTGCCCTTCAGTCAGGCCGGTGGCGCCTTGCTGTTCCACCTGCTGTCCAAGC
TCTATGAGCGCACCAGCGTGGTCGTCACCACCAACCTGTCGTTCTCAGAGTGGGCCAGTGTGTTCGGCGACGCCAAGATGACCACCGCGCTGCTGGACCG
GCTCACCCATCACTGCCACATCGTCGAGACCGGCAATCAGAGCTGGCGATTCAGGCACTCGACGGCACAACCGGCCGCCACCAAGTCCAACCGCATCAAA
ACACCCAAAGGAGCAGACCAGACAGTAGACTTATCCACAACCGAGCAGTCCATTTCTTCGTCAACCTAGTGGCTCAGTTTTGGACGTGACGCCTGGCTCA
GTTTTGCTCGTGATTCAACA
Protein section
ORF number : 2

 

ORF 1
LengthBeginEndStrandFusion ORF
1533 bp510 aa1171649+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MSTLSKLRRMVHRDHLSVREASRRLGISRNTATKWLEADEMVEPRYPKRAAIPSVLDPYKEQLSTWLKADSHRNKRERRGIKAMFRALRAMGYPGSRGPV
YEFAKRWQQAQSDAPTRMAFVPMSFEMGEAFQFDWSCEYLFVGGMRKRLEAAHTKLAASRAFMLTAYFSQAHEMLFDAHARAFAAFGGVPRRGIYDNMKT
AVDKVGQGKSRTVNARFEAMTGHYLFEPEFCNRAAGWEKGIVEKNVQDRRRGIWLEAAERRWPDLESLNAWLHQACLDAWHELPHPEWPELTIADVWQQE
QTHLMPNPAPFDGYVEQVARVTATSLIHYQRNRYSVPCEWAHATVSLRAYPDRLVVVGPSTDAPEPPVSLPRSFERGQTLYDWRHYISLLERKPGALRNG
APFKTMPEPLQHLQAHLLRHPGGDRVMAQVLMAITLHGLDDVLVAVELALQSGRVSADHVLNVLARLKEPEAVHSLPEAALTSLTLQEPPQADVSRYDRL
RQIQEGDHVQ

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
831 bp276 aa16392469+No
ORF function : Accessory Gene
AG : IS21 helper

ORF sequence :

MSNDIAAGLKGLSLHGMASAWPELLGIARLKSLDHEALLHQLIKAEGAHREVRSMAYQMRVARFPHHRDLAGFAFDQAQVDEALVRQLHELRFIDSAHNV
VFVGGPGTGKTHLATSLGVHAIRAHGKRVRFFSTVELVNMLEAEKAQGKAGQLAHRLMYVDLVILDEMGYLPFSQAGGALLFHLLSKLYERTSVVVTTNL
SFSEWASVFGDAKMTTALLDRLTHHCHIVETGNQSWRFRHSTAQPAATKSNRIKTPKGADQTVDLSTTEQSISSST

 

Blast result :
Comments
ISPa65 is 99% (transposase) and 96% (IS21 helper) aa similar to ISAav1.
References
1] Thomas Jové (2016) Direct submission.
2] Tada,T. and Kirikae (2015) Direct GenBank submission.