ISAisp7
- Family IS21
- Group
Isoform Synonym(s)
Accession number | Transposition | Origin | Host |
---|---|---|---|
CP003872 | ND | Acidovorax sp. | Acidovorax sp. KKS102 |
DNA section
IS Length : 2501 bp
Ends
IR Length : 21/28
IRL : tgttgatttccgcgcagaagtgacccactagcccccaggatttccatcca
IRR : tgttgattcccatgcaaacctgccccaccattttcatctcgatctaaccc
Insertion site
Left flank | Direct repeat | Right flank | DR Length |
---|---|---|---|
cgcacacagc | 0 | gagagcaagc | 0 |
DNA sequence
TGTTGATTTCCGCGCAGAAGTGACCCACTAGCCCCCAGGATTTCCATCCAAACCTGACCCACGTACTAACCCTAACCTGCTGCTTTGTTGAGCAGCAGGA
GACCAGGAGTGATAGACGTGGCAACACTGAGTGTCATCAGGCGCTGGGCCCTGCGAGAGCAGTTGTCCATCCGGGAGATCGCCCGCCGCACGGGCCTCTC
GCGCAACACCATCCGCAAGTACCTGCGCGCTGGCGAGGCCGAGCCGCACTACGCCAAGCGGGTCAGTCCATCCAAGCTCGATCCCTTCGCCTTGAAGCTC
GCCGGCTGGCTCAAGACGGAAGCTGGCCGATCCCGCAAGCAGCGGCGCACCATCAAGCAGATGTACTTGGATCTGCAGGCGCTCGGTTATGGCGGCTCCT
ACAACCGTGTGGCGGCCTTTGCGCGCCTCTGGCACGAGCAGCGCCTTGTGGCACAGCAGACCACTGGCCGTGGCACCTTCGTTCCCCTGACCTTCGGTCC
GGGTGAGGCCTTCCAGTTCGACTGGAGCGAGGACTGGGCTGTTCTCGCTGGCGTGCGCACCAAGCTGCAGGTAGCCCACTTCAAGCTCAGTCACAGCCGA
GCGTTCTACCTGCGCGCCTATCCGCTGCAGACGCACGAGATGCTGTTCGATGCCCACAACCATGCGTTCGTGGTCCTGGGTGGCGTGCCCCGCCGTGGCA
TCTACGACAACATGCGCACCGCTGTAGACCGGGTGCGCCGTGGCAAGGAGCGCGACGTCAATGCGCGCTTTGCGGCCATGGTCAGCCACTTCCTGTTCGA
GGCCGAGTTCTGCAACCCGGCCTCGGGCTGGGAGAAAGGCCAGGTGGAGAAGAACGTGCGCGATGCCCGCCATCGCCTGTGGCAGGTCGTGCCGCCATTC
CCAAGCCTTCCTGAGCTCAATGCATGGCTGCAGGAGCGCTGCGTGGCTCTGTGGCACGAGATCGCGCACGGCAAGCTGCCTGGCACCGTCGCGGATGTCT
GGGCCCAAGAGAAGGCAGCCTTGATGCCGATGTTCCGGCCCTTCGATGGCTTTGTGGAGCACACCAAGCGCGTCTCACCCACCTGCCTGGTCCACTTCGA
GCGCAATCGCTACAGCGTGCCAGCCCCGTATGCCAATCGGCCTGTGAGCTTGCGGGTCTACGCAGATCGTCTTGTGGTTGCGGCCGAAGGCCAGATCGTT
TGCGAGCACCAGCGCCTGATCGAGCGTAACCACCATGGTGCCGGGCAAACCGTGTACGACTGGCGCCACTACCTGGCGGTGCTGCAGCGCAAACCAGGAG
CTTTGCGCAACGGCGCCCCATTCCTGGAGTTGCCGCCGGCCTTCAAGCGACTGCAGGCCACCTTGCTCAAGCAAACCGGTGGCGACCGAGAGATGGTGGA
GGTTCTGGCTCTGGTGCTGCACCACGATGAACAGGCTGTGCTCGCTGCAGTGGAGTTGGCACTGGAGTCAGGGGCTGCCAGCAAGACGCACATCCTGAAC
GTGCTGCACCGCCTGCTGGACGGTAAGCCCGCTCCAGCACCGGTCACATCGCCGCAGGCCCTCAAGCTGGAGGTGGAACCCCAAGCCAATGTGCTTCGCT
ATGACCAATTGAGGGGTGTGCGCTATGCGTCATGACCCTGCCATCGCTTCCATCGTGATCATGCTACGCGAGCTCAAGATGCACGGCATGGCCCAGGCGG
TTGCGGAACTGGCTGAGCAAGGTGCGCCGGCCTTCGACGCAGCGCAGCCCATCCTGTCCCAACTGCTCAAGGCCGAGACCGCCGAGCGGGAGGTGCGCTC
GGTGGCCTACCAATTGAAGGTGGCCAGGTTCCCGGCGTACCGGGACCTGGCTGGGTTTGACTTCGGCCACAGTGAGGTGAACGAGGCACTGGTACGCCAG
CTGCACCGCTGCGAATTCTTGGAGAACGCCAACAACGTGGTGTTGGTGGGTGGCCCGGGTACGGGCAAGACCCACATCGCCACAGCCCTCGGGGTGCAAG
CCATTGAGCACCATCACCGCAGGGTGCGGTTCTTCTCCACGGTGGAGCTGGTCAATGCACTGGAAGAGGAGAAGGCCAACGGCAAGCCCGGACAGTTGGC
CCACCGGTTGGCCTATGCCGATCTGGTGATCCTGGATGAACTGGGCTACCTGCCATTCAGCACGTCAGGAGGAGCCTTGCTGTTCCACCTGCTGTCCAAG
CTGTACGAGCGCACCAGCGTTGTGATCACCACCAACCTGAGCTTCAGCGAATGGGCCAGTGTGTTCGGGGATGCCAAGATGACCACGGCACTGCTGGACC
GACTCACGCATCACTGCCATATTCTGGAAACGGGTAACGACAGCTACAGGTTCAAGAACAGCTCCGCACAGCAACCACCACAGACCACCAAGAAGGAGAA
GGCGACCAAGAACTTATCCACAACGTGAGCGTGAAGCTCACGAACCAGGGTGGGTTAGATCGAGATGAAAATGGTGGGGCAGGTTTGCATGGGAATCAAC
A
GACCAGGAGTGATAGACGTGGCAACACTGAGTGTCATCAGGCGCTGGGCCCTGCGAGAGCAGTTGTCCATCCGGGAGATCGCCCGCCGCACGGGCCTCTC
GCGCAACACCATCCGCAAGTACCTGCGCGCTGGCGAGGCCGAGCCGCACTACGCCAAGCGGGTCAGTCCATCCAAGCTCGATCCCTTCGCCTTGAAGCTC
GCCGGCTGGCTCAAGACGGAAGCTGGCCGATCCCGCAAGCAGCGGCGCACCATCAAGCAGATGTACTTGGATCTGCAGGCGCTCGGTTATGGCGGCTCCT
ACAACCGTGTGGCGGCCTTTGCGCGCCTCTGGCACGAGCAGCGCCTTGTGGCACAGCAGACCACTGGCCGTGGCACCTTCGTTCCCCTGACCTTCGGTCC
GGGTGAGGCCTTCCAGTTCGACTGGAGCGAGGACTGGGCTGTTCTCGCTGGCGTGCGCACCAAGCTGCAGGTAGCCCACTTCAAGCTCAGTCACAGCCGA
GCGTTCTACCTGCGCGCCTATCCGCTGCAGACGCACGAGATGCTGTTCGATGCCCACAACCATGCGTTCGTGGTCCTGGGTGGCGTGCCCCGCCGTGGCA
TCTACGACAACATGCGCACCGCTGTAGACCGGGTGCGCCGTGGCAAGGAGCGCGACGTCAATGCGCGCTTTGCGGCCATGGTCAGCCACTTCCTGTTCGA
GGCCGAGTTCTGCAACCCGGCCTCGGGCTGGGAGAAAGGCCAGGTGGAGAAGAACGTGCGCGATGCCCGCCATCGCCTGTGGCAGGTCGTGCCGCCATTC
CCAAGCCTTCCTGAGCTCAATGCATGGCTGCAGGAGCGCTGCGTGGCTCTGTGGCACGAGATCGCGCACGGCAAGCTGCCTGGCACCGTCGCGGATGTCT
GGGCCCAAGAGAAGGCAGCCTTGATGCCGATGTTCCGGCCCTTCGATGGCTTTGTGGAGCACACCAAGCGCGTCTCACCCACCTGCCTGGTCCACTTCGA
GCGCAATCGCTACAGCGTGCCAGCCCCGTATGCCAATCGGCCTGTGAGCTTGCGGGTCTACGCAGATCGTCTTGTGGTTGCGGCCGAAGGCCAGATCGTT
TGCGAGCACCAGCGCCTGATCGAGCGTAACCACCATGGTGCCGGGCAAACCGTGTACGACTGGCGCCACTACCTGGCGGTGCTGCAGCGCAAACCAGGAG
CTTTGCGCAACGGCGCCCCATTCCTGGAGTTGCCGCCGGCCTTCAAGCGACTGCAGGCCACCTTGCTCAAGCAAACCGGTGGCGACCGAGAGATGGTGGA
GGTTCTGGCTCTGGTGCTGCACCACGATGAACAGGCTGTGCTCGCTGCAGTGGAGTTGGCACTGGAGTCAGGGGCTGCCAGCAAGACGCACATCCTGAAC
GTGCTGCACCGCCTGCTGGACGGTAAGCCCGCTCCAGCACCGGTCACATCGCCGCAGGCCCTCAAGCTGGAGGTGGAACCCCAAGCCAATGTGCTTCGCT
ATGACCAATTGAGGGGTGTGCGCTATGCGTCATGACCCTGCCATCGCTTCCATCGTGATCATGCTACGCGAGCTCAAGATGCACGGCATGGCCCAGGCGG
TTGCGGAACTGGCTGAGCAAGGTGCGCCGGCCTTCGACGCAGCGCAGCCCATCCTGTCCCAACTGCTCAAGGCCGAGACCGCCGAGCGGGAGGTGCGCTC
GGTGGCCTACCAATTGAAGGTGGCCAGGTTCCCGGCGTACCGGGACCTGGCTGGGTTTGACTTCGGCCACAGTGAGGTGAACGAGGCACTGGTACGCCAG
CTGCACCGCTGCGAATTCTTGGAGAACGCCAACAACGTGGTGTTGGTGGGTGGCCCGGGTACGGGCAAGACCCACATCGCCACAGCCCTCGGGGTGCAAG
CCATTGAGCACCATCACCGCAGGGTGCGGTTCTTCTCCACGGTGGAGCTGGTCAATGCACTGGAAGAGGAGAAGGCCAACGGCAAGCCCGGACAGTTGGC
CCACCGGTTGGCCTATGCCGATCTGGTGATCCTGGATGAACTGGGCTACCTGCCATTCAGCACGTCAGGAGGAGCCTTGCTGTTCCACCTGCTGTCCAAG
CTGTACGAGCGCACCAGCGTTGTGATCACCACCAACCTGAGCTTCAGCGAATGGGCCAGTGTGTTCGGGGATGCCAAGATGACCACGGCACTGCTGGACC
GACTCACGCATCACTGCCATATTCTGGAAACGGGTAACGACAGCTACAGGTTCAAGAACAGCTCCGCACAGCAACCACCACAGACCACCAAGAAGGAGAA
GGCGACCAAGAACTTATCCACAACGTGAGCGTGAAGCTCACGAACCAGGGTGGGTTAGATCGAGATGAAAATGGTGGGGCAGGTTTGCATGGGAATCAAC
A
Protein section
ORF number : 2
ORF 1
Length | Begin | End | Strand | Fusion ORF | |
---|---|---|---|---|---|
1527 bp | 508 aa | 109 | 1635 | + | No |
Chemistry : DDE
ORF sequence :
MIDVATLSVIRRWALREQLSIREIARRTGLSRNTIRKYLRAGEAEPHYAKRVSPSKLDPFALKLAGWLKTEAGRSRKQRRTIKQMYLDLQALGYGGSYNR
VAAFARLWHEQRLVAQQTTGRGTFVPLTFGPGEAFQFDWSEDWAVLAGVRTKLQVAHFKLSHSRAFYLRAYPLQTHEMLFDAHNHAFVVLGGVPRRGIYD
NMRTAVDRVRRGKERDVNARFAAMVSHFLFEAEFCNPASGWEKGQVEKNVRDARHRLWQVVPPFPSLPELNAWLQERCVALWHEIAHGKLPGTVADVWAQ
EKAALMPMFRPFDGFVEHTKRVSPTCLVHFERNRYSVPAPYANRPVSLRVYADRLVVAAEGQIVCEHQRLIERNHHGAGQTVYDWRHYLAVLQRKPGALR
NGAPFLELPPAFKRLQATLLKQTGGDREMVEVLALVLHHDEQAVLAAVELALESGAASKTHILNVLHRLLDGKPAPAPVTSPQALKLEVEPQANVLRYDQ
LRGVRYAS*
VAAFARLWHEQRLVAQQTTGRGTFVPLTFGPGEAFQFDWSEDWAVLAGVRTKLQVAHFKLSHSRAFYLRAYPLQTHEMLFDAHNHAFVVLGGVPRRGIYD
NMRTAVDRVRRGKERDVNARFAAMVSHFLFEAEFCNPASGWEKGQVEKNVRDARHRLWQVVPPFPSLPELNAWLQERCVALWHEIAHGKLPGTVADVWAQ
EKAALMPMFRPFDGFVEHTKRVSPTCLVHFERNRYSVPAPYANRPVSLRVYADRLVVAAEGQIVCEHQRLIERNHHGAGQTVYDWRHYLAVLQRKPGALR
NGAPFLELPPAFKRLQATLLKQTGGDREMVEVLALVLHHDEQAVLAAVELALESGAASKTHILNVLHRLLDGKPAPAPVTSPQALKLEVEPQANVLRYDQ
LRGVRYAS*
Blast result :ORF 2
Length | Begin | End | Strand | Fusion ORF | |
---|---|---|---|---|---|
804 bp | 267 aa | 1625 | 2428 | + | No |
AG : IS21 helper
ORF sequence :
MRHDPAIASIVIMLRELKMHGMAQAVAELAEQGAPAFDAAQPILSQLLKAETAEREVRSVAYQLKVARFPAYRDLAGFDFGHSEVNEALVRQLHRCEFLE
NANNVVLVGGPGTGKTHIATALGVQAIEHHHRRVRFFSTVELVNALEEEKANGKPGQLAHRLAYADLVILDELGYLPFSTSGGALLFHLLSKLYERTSVV
ITTNLSFSEWASVFGDAKMTTALLDRLTHHCHILETGNDSYRFKNSSAQQPPQTTKKEKATKNLSTT
NANNVVLVGGPGTGKTHIATALGVQAIEHHHRRVRFFSTVELVNALEEEKANGKPGQLAHRLAYADLVILDELGYLPFSTSGGALLFHLLSKLYERTSVV
ITTNLSFSEWASVFGDAKMTTALLDRLTHHCHILETGNDSYRFKNSSAQQPPQTTKKEKATKNLSTT
Blast result :
Comments
ISAisp7 is 97% (transposase) and 99% (IS21 helper) aa similar to ISAlde1.
References
1] Thomas Jové (2016) Direct submission.
2] Ohtsubo Y, Maruyama F, Mitsui H, Nagata Y, Tsuda M. J Bacteriol. (2012) Dec;194(24):6970-1.
2] Ohtsubo Y, Maruyama F, Mitsui H, Nagata Y, Tsuda M. J Bacteriol. (2012) Dec;194(24):6970-1.