ISAisp7

  • Family IS21
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
CP003872 ND Acidovorax sp.
Acidovorax sp. KKS102
DNA section
IS Length : 2501 bp

Ends


IR Length : 21/28

IRL : tgttgatttccgcgcagaagtgacccactagcccccaggatttccatcca
IRR : tgttgattcccatgcaaacctgccccaccattttcatctcgatctaaccc

Insertion site


Left flankDirect repeatRight flankDR Length
cgcacacagc0gagagcaagc0

DNA sequence

TGTTGATTTCCGCGCAGAAGTGACCCACTAGCCCCCAGGATTTCCATCCAAACCTGACCCACGTACTAACCCTAACCTGCTGCTTTGTTGAGCAGCAGGA
GACCAGGAGTGATAGACGTGGCAACACTGAGTGTCATCAGGCGCTGGGCCCTGCGAGAGCAGTTGTCCATCCGGGAGATCGCCCGCCGCACGGGCCTCTC
GCGCAACACCATCCGCAAGTACCTGCGCGCTGGCGAGGCCGAGCCGCACTACGCCAAGCGGGTCAGTCCATCCAAGCTCGATCCCTTCGCCTTGAAGCTC
GCCGGCTGGCTCAAGACGGAAGCTGGCCGATCCCGCAAGCAGCGGCGCACCATCAAGCAGATGTACTTGGATCTGCAGGCGCTCGGTTATGGCGGCTCCT
ACAACCGTGTGGCGGCCTTTGCGCGCCTCTGGCACGAGCAGCGCCTTGTGGCACAGCAGACCACTGGCCGTGGCACCTTCGTTCCCCTGACCTTCGGTCC
GGGTGAGGCCTTCCAGTTCGACTGGAGCGAGGACTGGGCTGTTCTCGCTGGCGTGCGCACCAAGCTGCAGGTAGCCCACTTCAAGCTCAGTCACAGCCGA
GCGTTCTACCTGCGCGCCTATCCGCTGCAGACGCACGAGATGCTGTTCGATGCCCACAACCATGCGTTCGTGGTCCTGGGTGGCGTGCCCCGCCGTGGCA
TCTACGACAACATGCGCACCGCTGTAGACCGGGTGCGCCGTGGCAAGGAGCGCGACGTCAATGCGCGCTTTGCGGCCATGGTCAGCCACTTCCTGTTCGA
GGCCGAGTTCTGCAACCCGGCCTCGGGCTGGGAGAAAGGCCAGGTGGAGAAGAACGTGCGCGATGCCCGCCATCGCCTGTGGCAGGTCGTGCCGCCATTC
CCAAGCCTTCCTGAGCTCAATGCATGGCTGCAGGAGCGCTGCGTGGCTCTGTGGCACGAGATCGCGCACGGCAAGCTGCCTGGCACCGTCGCGGATGTCT
GGGCCCAAGAGAAGGCAGCCTTGATGCCGATGTTCCGGCCCTTCGATGGCTTTGTGGAGCACACCAAGCGCGTCTCACCCACCTGCCTGGTCCACTTCGA
GCGCAATCGCTACAGCGTGCCAGCCCCGTATGCCAATCGGCCTGTGAGCTTGCGGGTCTACGCAGATCGTCTTGTGGTTGCGGCCGAAGGCCAGATCGTT
TGCGAGCACCAGCGCCTGATCGAGCGTAACCACCATGGTGCCGGGCAAACCGTGTACGACTGGCGCCACTACCTGGCGGTGCTGCAGCGCAAACCAGGAG
CTTTGCGCAACGGCGCCCCATTCCTGGAGTTGCCGCCGGCCTTCAAGCGACTGCAGGCCACCTTGCTCAAGCAAACCGGTGGCGACCGAGAGATGGTGGA
GGTTCTGGCTCTGGTGCTGCACCACGATGAACAGGCTGTGCTCGCTGCAGTGGAGTTGGCACTGGAGTCAGGGGCTGCCAGCAAGACGCACATCCTGAAC
GTGCTGCACCGCCTGCTGGACGGTAAGCCCGCTCCAGCACCGGTCACATCGCCGCAGGCCCTCAAGCTGGAGGTGGAACCCCAAGCCAATGTGCTTCGCT
ATGACCAATTGAGGGGTGTGCGCTATGCGTCATGACCCTGCCATCGCTTCCATCGTGATCATGCTACGCGAGCTCAAGATGCACGGCATGGCCCAGGCGG
TTGCGGAACTGGCTGAGCAAGGTGCGCCGGCCTTCGACGCAGCGCAGCCCATCCTGTCCCAACTGCTCAAGGCCGAGACCGCCGAGCGGGAGGTGCGCTC
GGTGGCCTACCAATTGAAGGTGGCCAGGTTCCCGGCGTACCGGGACCTGGCTGGGTTTGACTTCGGCCACAGTGAGGTGAACGAGGCACTGGTACGCCAG
CTGCACCGCTGCGAATTCTTGGAGAACGCCAACAACGTGGTGTTGGTGGGTGGCCCGGGTACGGGCAAGACCCACATCGCCACAGCCCTCGGGGTGCAAG
CCATTGAGCACCATCACCGCAGGGTGCGGTTCTTCTCCACGGTGGAGCTGGTCAATGCACTGGAAGAGGAGAAGGCCAACGGCAAGCCCGGACAGTTGGC
CCACCGGTTGGCCTATGCCGATCTGGTGATCCTGGATGAACTGGGCTACCTGCCATTCAGCACGTCAGGAGGAGCCTTGCTGTTCCACCTGCTGTCCAAG
CTGTACGAGCGCACCAGCGTTGTGATCACCACCAACCTGAGCTTCAGCGAATGGGCCAGTGTGTTCGGGGATGCCAAGATGACCACGGCACTGCTGGACC
GACTCACGCATCACTGCCATATTCTGGAAACGGGTAACGACAGCTACAGGTTCAAGAACAGCTCCGCACAGCAACCACCACAGACCACCAAGAAGGAGAA
GGCGACCAAGAACTTATCCACAACGTGAGCGTGAAGCTCACGAACCAGGGTGGGTTAGATCGAGATGAAAATGGTGGGGCAGGTTTGCATGGGAATCAAC
A
Protein section
ORF number : 2

 

ORF 1
LengthBeginEndStrandFusion ORF
1527 bp508 aa1091635+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MIDVATLSVIRRWALREQLSIREIARRTGLSRNTIRKYLRAGEAEPHYAKRVSPSKLDPFALKLAGWLKTEAGRSRKQRRTIKQMYLDLQALGYGGSYNR
VAAFARLWHEQRLVAQQTTGRGTFVPLTFGPGEAFQFDWSEDWAVLAGVRTKLQVAHFKLSHSRAFYLRAYPLQTHEMLFDAHNHAFVVLGGVPRRGIYD
NMRTAVDRVRRGKERDVNARFAAMVSHFLFEAEFCNPASGWEKGQVEKNVRDARHRLWQVVPPFPSLPELNAWLQERCVALWHEIAHGKLPGTVADVWAQ
EKAALMPMFRPFDGFVEHTKRVSPTCLVHFERNRYSVPAPYANRPVSLRVYADRLVVAAEGQIVCEHQRLIERNHHGAGQTVYDWRHYLAVLQRKPGALR
NGAPFLELPPAFKRLQATLLKQTGGDREMVEVLALVLHHDEQAVLAAVELALESGAASKTHILNVLHRLLDGKPAPAPVTSPQALKLEVEPQANVLRYDQ
LRGVRYAS*

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
804 bp267 aa16252428+No
ORF function : Accessory Gene
AG : IS21 helper

ORF sequence :

MRHDPAIASIVIMLRELKMHGMAQAVAELAEQGAPAFDAAQPILSQLLKAETAEREVRSVAYQLKVARFPAYRDLAGFDFGHSEVNEALVRQLHRCEFLE
NANNVVLVGGPGTGKTHIATALGVQAIEHHHRRVRFFSTVELVNALEEEKANGKPGQLAHRLAYADLVILDELGYLPFSTSGGALLFHLLSKLYERTSVV
ITTNLSFSEWASVFGDAKMTTALLDRLTHHCHILETGNDSYRFKNSSAQQPPQTTKKEKATKNLSTT

 

Blast result :
Comments
ISAisp7 is 97% (transposase) and 99% (IS21 helper) aa similar to ISAlde1.
References
1] Thomas Jové (2016) Direct submission.
2] Ohtsubo Y, Maruyama F, Mitsui H, Nagata Y, Tsuda M. J Bacteriol. (2012) Dec;194(24):6970-1.