ISVapa4
- Family IS21
- Group
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| CP001635 | ND | Variovorax paradoxus | Variovorax paradoxus S110 |
DNA section
IS Length : 2624 bp
Ends
IR Length : 20/27
IRL : TGTTGGCGCCGACCGGAAACTGAGCCAGTTTTGTAGGTAGTGCCGAAGTA
IRR : TGTCCGCGCCGAAGTAGAACTGAGCCACCGCGCCGCTTGAATCGTGAGCC
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| tgcagtcgac | gccccggtgt | 0 |
DNA sequence
TGTTGGCGCCGACCGGAAACTGAGCCAGTTTTGTAGGTAGTGCCGAAGTAAAAGTGAGCCAGGTGTTCAACCTACCCTGCTGTTTATTTAAGCAGCGGGG
TTCGAGGAGTGATCACCATGGACATGATTGGCAGGATCCGACGGCTGCACGCGAGGGACAAGCTGTCGGAGCGCGAGATAGCGCGCATGACAGGGCTGTC
GCGCAACACGGTGTCAAAGTGGCTGCGCGCACCGGCAGCCGAGGCGCCGAAGTACCGGCGCGAAGCAAGGCCGAACAAGCTGAGTGCATTCGAGACAGCG
CTGAAGCAGGCATTGACGGCCGATGCGCGACGGCCCAAGCACGAGCGGCGCACGGCACGCGCGCTGCACGCTGAGATCAAGGCGGCGGGCTACGACGGCG
GGTACTCGGCCATCACTGACTTCGTTCGAGCGTGGCGCCAAGGCGAGGGACAGGCAGTCAACGTCACCGCGTTCGTTCCACTGACCTTCGAGCTCGGCGA
GGCCTTCCAGTTCGATTGGAGCGAAGAAGGCCTGGTCGTCGGCGGCATCTATTACCGCATGCAGGTGTCGCACCTGAAGCTGTGCGCGAGCCGTGCGTTC
TGGCTGGTGGCGTACCCGAGCCAAGGTCACGAGATGCTGTTCGACGCCCACACGAGGTCGTTTGCAGCGCTGGGTGGTGTGGCGCGCCGCGGGATCTACG
ACAACATGCGCACCGCCGTCGATAAGGTTCACAAGGGTAAGGGTCGCACCGTCAATGCCCGCTTCGCCGTGATGTGCGCGCACTACCTGTACGACCCCGA
CTTCTGCAACGTCGCCTCGGGCTGGGAGAAGGGGCGCGTGGAGAAGAACGTACAGGACAGCCGTCGGCGCATCTGGATCGAGGCGGGCCGGCGCCGCTTC
GGCTCCTTCGTCGAACTCAATGCCTGGTTGGCGGAGCGATGCCGAGCGCTGTGGAACGAGGTGCGCCACCCCGAGCACAGCCAGTTCAGCGTGGCCGAGA
TGCTCGAGCACGAGCGTCCCCACCTGATGCCCATGCCTGAGCCCTTCGATGGCTACGTAGAGAAGCCTGCCCGGGTGTCGAGCACGTGCCTGGTGTCGGT
GGCACGTAACCGCTACTCGGTGCCCTGCGAGCTGGCCGGGCAGATGGTCAGCACGCGGCTGTATCCGGGCGGCATCGTGGTTGTTGCCGATGATGCGGTG
GTTGCGCGACACGAGCGCCTGAGCGCCTCTGGCGGCACCCGCTACGACTGGCAGCACTACATTCCGCTCATCCAGCGCAAGCCTGGTGCATTGAGGAACG
GTGCGCCCTTCGCAGACATGCCCGAGGCACTGCAGCAACTGCGCCGCGGCCTGCTGCGCCAGGCCGGTGGAGATCGCGTGATGGCGCAGGTGTTGGCCAT
CGTGCCGACGGCGGGGCTGGACGCGGTGATCGTGGCGGTCGAGTTGGCGCTGCAGAGTGGTCCGCCATCGGGTCGGGTCAGCGTGGAGCACGTCGTCAAC
GTCCTCGGGCGGCTCACCGCACCGGCAGCACCGCAGAGTGCGCAGACGGCGCTGCAGATCGTCACGCCGCCGCTGGCCAACACGGCCCGATACGACGGCT
TGCGTGGACAGGAGGTGGACCATGCGTGACGTGACAGTCGAACTCAAGGCGTTGCGCCTGCACGGCATGGCCAGTGCCTGGTGCGATCTGGCGGAGCAAG
GTGCCGCGTCCGGCTTGGATGCCTCTCGCTGGCTGGTCGAGCACCTGCTGCAGGCGGAGACCACGGACCGTTCGATGCGTTCGGTCCAGCACCAGATGAA
CTCGGCGCGCTTCCCCGTGCACCGGGATCTGGCCGGCTTCGACTTCGATGCGTCGGTGGTCGACCAGAAGCTGGTCCACCAGCTCGCGACGCTGGAGTTC
ACGGAGGCCGCGCACAACGTGGTCTTGGTCGGTGGTCCGGGCACAGGCAAGACCCACCTGGCAACGGCTCTGGGCGTGTCGGGCATCGTGCGCCACAGCA
AGCGCGTGCGGTTCTACTCGACGGTCGATCTGGTCAATGCACTGGAGCAGGAGAAGGCCCAGGGCAAGGCCGGGCGTATTGCGCTGAGCCTGCTGCGAAT
GGATTTGGTGATCCTGGACGAACTGGGCTACTTGCCGTTCAGCCAGGCCGGCGGCGCGCTGCTGTTCCACCTGCTGTCCAAGCTGTACGAGCACACCAGC
GTGGTGATCACCACCAACCTGGACTTCGCCGAATGGTCGACGGTGTTCGGCGACGCGAAGATGACGACCGCCTTGCTCGACCGCCTCACGCACCACTGTC
ACATCGTGGAGACGGGCAACGAGAGCCATCGGTTCCTGCACAGCAGCTCCACCGCGAAGAAGCGCATCAAGGCCAGGGAGCAGACCCGCAAGACAGGCGC
CAAGGCCGATCCAACAGACGCCGTTGATTGACGGCCGTCGCGCGTGACAAGGCGCTACGGGCTTCGCCCTTCGCGCCTTATGAGTTCGATCACAAGAAGC
GAAAGAAGGTCACAGAACAAGGCAAGACATACACTTATCCACAGTCGACCATTGCTGCGTCGGCTTCCACCCCTGGCTCACGATTCAAGCGGCGCGGTGG
CTCAGTTCTACTTCGGCGCGGACA
TTCGAGGAGTGATCACCATGGACATGATTGGCAGGATCCGACGGCTGCACGCGAGGGACAAGCTGTCGGAGCGCGAGATAGCGCGCATGACAGGGCTGTC
GCGCAACACGGTGTCAAAGTGGCTGCGCGCACCGGCAGCCGAGGCGCCGAAGTACCGGCGCGAAGCAAGGCCGAACAAGCTGAGTGCATTCGAGACAGCG
CTGAAGCAGGCATTGACGGCCGATGCGCGACGGCCCAAGCACGAGCGGCGCACGGCACGCGCGCTGCACGCTGAGATCAAGGCGGCGGGCTACGACGGCG
GGTACTCGGCCATCACTGACTTCGTTCGAGCGTGGCGCCAAGGCGAGGGACAGGCAGTCAACGTCACCGCGTTCGTTCCACTGACCTTCGAGCTCGGCGA
GGCCTTCCAGTTCGATTGGAGCGAAGAAGGCCTGGTCGTCGGCGGCATCTATTACCGCATGCAGGTGTCGCACCTGAAGCTGTGCGCGAGCCGTGCGTTC
TGGCTGGTGGCGTACCCGAGCCAAGGTCACGAGATGCTGTTCGACGCCCACACGAGGTCGTTTGCAGCGCTGGGTGGTGTGGCGCGCCGCGGGATCTACG
ACAACATGCGCACCGCCGTCGATAAGGTTCACAAGGGTAAGGGTCGCACCGTCAATGCCCGCTTCGCCGTGATGTGCGCGCACTACCTGTACGACCCCGA
CTTCTGCAACGTCGCCTCGGGCTGGGAGAAGGGGCGCGTGGAGAAGAACGTACAGGACAGCCGTCGGCGCATCTGGATCGAGGCGGGCCGGCGCCGCTTC
GGCTCCTTCGTCGAACTCAATGCCTGGTTGGCGGAGCGATGCCGAGCGCTGTGGAACGAGGTGCGCCACCCCGAGCACAGCCAGTTCAGCGTGGCCGAGA
TGCTCGAGCACGAGCGTCCCCACCTGATGCCCATGCCTGAGCCCTTCGATGGCTACGTAGAGAAGCCTGCCCGGGTGTCGAGCACGTGCCTGGTGTCGGT
GGCACGTAACCGCTACTCGGTGCCCTGCGAGCTGGCCGGGCAGATGGTCAGCACGCGGCTGTATCCGGGCGGCATCGTGGTTGTTGCCGATGATGCGGTG
GTTGCGCGACACGAGCGCCTGAGCGCCTCTGGCGGCACCCGCTACGACTGGCAGCACTACATTCCGCTCATCCAGCGCAAGCCTGGTGCATTGAGGAACG
GTGCGCCCTTCGCAGACATGCCCGAGGCACTGCAGCAACTGCGCCGCGGCCTGCTGCGCCAGGCCGGTGGAGATCGCGTGATGGCGCAGGTGTTGGCCAT
CGTGCCGACGGCGGGGCTGGACGCGGTGATCGTGGCGGTCGAGTTGGCGCTGCAGAGTGGTCCGCCATCGGGTCGGGTCAGCGTGGAGCACGTCGTCAAC
GTCCTCGGGCGGCTCACCGCACCGGCAGCACCGCAGAGTGCGCAGACGGCGCTGCAGATCGTCACGCCGCCGCTGGCCAACACGGCCCGATACGACGGCT
TGCGTGGACAGGAGGTGGACCATGCGTGACGTGACAGTCGAACTCAAGGCGTTGCGCCTGCACGGCATGGCCAGTGCCTGGTGCGATCTGGCGGAGCAAG
GTGCCGCGTCCGGCTTGGATGCCTCTCGCTGGCTGGTCGAGCACCTGCTGCAGGCGGAGACCACGGACCGTTCGATGCGTTCGGTCCAGCACCAGATGAA
CTCGGCGCGCTTCCCCGTGCACCGGGATCTGGCCGGCTTCGACTTCGATGCGTCGGTGGTCGACCAGAAGCTGGTCCACCAGCTCGCGACGCTGGAGTTC
ACGGAGGCCGCGCACAACGTGGTCTTGGTCGGTGGTCCGGGCACAGGCAAGACCCACCTGGCAACGGCTCTGGGCGTGTCGGGCATCGTGCGCCACAGCA
AGCGCGTGCGGTTCTACTCGACGGTCGATCTGGTCAATGCACTGGAGCAGGAGAAGGCCCAGGGCAAGGCCGGGCGTATTGCGCTGAGCCTGCTGCGAAT
GGATTTGGTGATCCTGGACGAACTGGGCTACTTGCCGTTCAGCCAGGCCGGCGGCGCGCTGCTGTTCCACCTGCTGTCCAAGCTGTACGAGCACACCAGC
GTGGTGATCACCACCAACCTGGACTTCGCCGAATGGTCGACGGTGTTCGGCGACGCGAAGATGACGACCGCCTTGCTCGACCGCCTCACGCACCACTGTC
ACATCGTGGAGACGGGCAACGAGAGCCATCGGTTCCTGCACAGCAGCTCCACCGCGAAGAAGCGCATCAAGGCCAGGGAGCAGACCCGCAAGACAGGCGC
CAAGGCCGATCCAACAGACGCCGTTGATTGACGGCCGTCGCGCGTGACAAGGCGCTACGGGCTTCGCCCTTCGCGCCTTATGAGTTCGATCACAAGAAGC
GAAAGAAGGTCACAGAACAAGGCAAGACATACACTTATCCACAGTCGACCATTGCTGCGTCGGCTTCCACCCCTGGCTCACGATTCAAGCGGCGCGGTGG
CTCAGTTCTACTTCGGCGCGGACA
Protein section
ORF number : 2
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1512 bp | 503 aa | 118 | 1629 | + | No |
Chemistry : DDE
ORF sequence :
MDMIGRIRRLHARDKLSEREIARMTGLSRNTVSKWLRAPAAEAPKYRREARPNKLSAFETALKQALTADARRPKHERRTARALHAEIKAAGYDGGYSAIT
DFVRAWRQGEGQAVNVTAFVPLTFELGEAFQFDWSEEGLVVGGIYYRMQVSHLKLCASRAFWLVAYPSQGHEMLFDAHTRSFAALGGVARRGIYDNMRTA
VDKVHKGKGRTVNARFAVMCAHYLYDPDFCNVASGWEKGRVEKNVQDSRRRIWIEAGRRRFGSFVELNAWLAERCRALWNEVRHPEHSQFSVAEMLEHER
PHLMPMPEPFDGYVEKPARVSSTCLVSVARNRYSVPCELAGQMVSTRLYPGGIVVVADDAVVARHERLSASGGTRYDWQHYIPLIQRKPGALRNGAPFAD
MPEALQQLRRGLLRQAGGDRVMAQVLAIVPTAGLDAVIVAVELALQSGPPSGRVSVEHVVNVLGRLTAPAAPQSAQTALQIVTPPLANTARYDGLRGQEV
DHA
DFVRAWRQGEGQAVNVTAFVPLTFELGEAFQFDWSEEGLVVGGIYYRMQVSHLKLCASRAFWLVAYPSQGHEMLFDAHTRSFAALGGVARRGIYDNMRTA
VDKVHKGKGRTVNARFAVMCAHYLYDPDFCNVASGWEKGRVEKNVQDSRRRIWIEAGRRRFGSFVELNAWLAERCRALWNEVRHPEHSQFSVAEMLEHER
PHLMPMPEPFDGYVEKPARVSSTCLVSVARNRYSVPCELAGQMVSTRLYPGGIVVVADDAVVARHERLSASGGTRYDWQHYIPLIQRKPGALRNGAPFAD
MPEALQQLRRGLLRQAGGDRVMAQVLAIVPTAGLDAVIVAVELALQSGPPSGRVSVEHVVNVLGRLTAPAAPQSAQTALQIVTPPLANTARYDGLRGQEV
DHA
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 810 bp | 254 aa | 1622 | 2431 | + | No |
AG : IS21 helper
ORF sequence :
MRDVTVELKALRLHGMASAWCDLAEQGAASGLDASRWLVEHLLQAETTDRSMRSVQHQMNSARFPVHRDLAGFDFDASVVDQKLVHQLATLEFTEAAHNV
VLVGGPGTGKTHLATALGVSGIVRHSKRVRFYSTVDLVNALEQEKAQGKAGRIALSLLRMDLVILDELGYLPFSQAGGALLFHLLSKLYEHTSVVITTNL
DFAEWSTVFGDAKMTTALLDRLTHHCHIVETGNESHRFLHSSSTAKKRIKAREQTRKTGAKADPTDAVD
VLVGGPGTGKTHLATALGVSGIVRHSKRVRFYSTVDLVNALEQEKAQGKAGRIALSLLRMDLVILDELGYLPFSQAGGALLFHLLSKLYEHTSVVITTNL
DFAEWSTVFGDAKMTTALLDRLTHHCHIVETGNESHRFLHSSSTAKKRIKAREQTRKTGAKADPTDAVD
Blast result :
Comments
ISVapa4 is 96% (transposase) and 94% (IS21 helper) aa similar to ISVapa2.
References
1] Thomas Jové (2016) Direct submission.
2] Lucas,S., Copeland,A., Lapidus,A., Glavina del Rio,T., Tice,H., Bruce,D., Goodwin,L., Pitluck,S., Chertkov,O., Brettin,T., Detter,J.C., Han,C., Larimer,F., Land,M., Hauser,L., Kyrpides,N., Ovchinnikova,G., Orwin,P., Leadbetter,J.R., Spain,J.C. and Han,J.I. (2014) Direct GenBank submission.
2] Lucas,S., Copeland,A., Lapidus,A., Glavina del Rio,T., Tice,H., Bruce,D., Goodwin,L., Pitluck,S., Chertkov,O., Brettin,T., Detter,J.C., Han,C., Larimer,F., Land,M., Hauser,L., Kyrpides,N., Ovchinnikova,G., Orwin,P., Leadbetter,J.R., Spain,J.C. and Han,J.I. (2014) Direct GenBank submission.