ISfinder

ISKpn37

Family IS3
Group IS3

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
KU318421	ND	Klebsiella pneumoniae	Klebsiella pneumoniae Klebsiella oxytoca strain CAV1015 plasmid pKPC_UVA02 Enterobacter lignolyticus SCF1 Escherichia coli strain D8

DNA section

IS Length : 1253 bp

Ends

IR Length : 23/27

IRL : CTGTAGTGGATCGGTAATTCAGGACACCTCGAAATCCTGTTAATGTTAAA
IRR : CTGTAGTGGATCACAAAATCAGGACACCGGGATAATCTGTTTCCACTTTT

Insertion site

Left flank	Direct repeat	Right flank	DR Length

DNA sequence

TGTAGTGGATCGGTAATTCAGGACACCTCGAAATCCTGTTAATGTTAAAACAGTGAAAATGAGGTGATGCATGATCAAAACTCGTCGGACTAAACGTACC
TTTTCCCCGGAGTTCAAGCTTGAAGCTATCGAACAGGTGGTTAAATACCAGCGTGATGTCAGAGAAGTCGCGCTGGCACTCGAGCTCAACCCTGACCATT
TGCGTAAATGGATACGGTTGTATAAGCAGGAACTTCAGGGTATTGAGCCCGCTGGTAATGCTATTACCCCTGAACAACGCGAAATTCAGCAGCTTAAAGC
GCGGATAAAGCGCCTTGAGATGGAAAAAGAAATACTAAAGCAGGCTGCCGTGCTGATGAGCGAAATCCCCGGGAAGCCGTCGCGCTAATCACACGGCTGA
AAGCAAAGTGGCCAGTGCGGGTTCTTTGTCATTTATTCGGTATTCACCGTAGCGTTTATTACGCGCAGGTGAAGCGTCCTGTTAATGTGCAAAGAATTGA
ATTACGAAGTCGGGTGAGGGCTTTCCATGCTCTCAGTCGTGGCGCAGCCGGTAGCCGGGCAATCAGTCAGATGTTGCGCCAGAGTGGCGTTGATGCAGGC
CGGTGGCTGGCACGACGACTGATGCGGGAATGCGGGCTGACAAGTCGACAGCCGGTTAAACATCACTACCGGGTAAACGAAGACAAAAGTCCGCCATTGC
CAAATTTACTGAACCGGCAATTTAACCCCGCCGCACCAAACCGCGTCTGGTGCGGCGACATCAGTTTTATTCGCCTGCAGGACAAATGGTGTTATCTCGC
GCTCGTTGTTGATTTATATTCACGGCGGATTATCGGTTCAGCCCTCTCATTAACCGCTGATGCTGAGTTGGTGTGTAGGGCTTTGCGTAATGCACTGGAA
ACACGACCGCGTGATGGCCGCCTGCTGTTTCATTCAGATCAAGGTGTTCAGTATAAAAGCAATAAATACAGAAAGTTACTCTGGCGTTATGGGGTAATGC
AAAGTATGAGTCGCAGAGGGAATTGCCTGGATAATTCGCCGATGGAAAGAGTCTTTCGCAGCCTTAAAAGTGAATGGCTTCCGAAAGGTGGTTATGGTGA
TTTTAGCCATTCGGTACGCGATATAAACCAGTGGATTAATGGTTATTACAACGTTTATCGACCTCACACGAACAACGATGGTTTACCGCCTTGCTTACAT
GAAGAAAAGTGGAAACAGATTATCCCGGTGTCCTGATTTTGTGATCCACTACA

Recoding section

Recoding by frameshift
Frame -1
Type translational
Experimentally demonstrated No

Stimulators :

Shine-Dalgarno sequence :
Secondary structure :

Recoding motif :

Protein section

ORF number : 3

ORF 1

Length		Begin	End	Strand	Fusion ORF
318 bp	105 aa	71	388	+	No

ORF function : Transposase
Description : First part of the transposase

ORF sequence :

MIKTRRTKRTFSPEFKLEAIEQVVKYQRDVREVALALELNPDHLRKWIRLYKQELQGIEPAGNAITPEQREIQQLKARIKRLEMEKEILKQAAVLMSEIP
GKPSR

Blast result :

ORF 2

Length		Begin	End	Strand	Fusion ORF
918 bp	305 aa	319	1236	+	No

ORF function : Transposase
Description : Second part of the transposase

ORF sequence :

DGKRNTKAGCRADERNPREAVALITRLKAKWPVRVLCHLFGIHRSVYYAQVKRPVNVQRIELRSRVRAFHALSRGAAGSRAISQMLRQSGVDAGRWLARR
LMRECGLTSRQPVKHHYRVNEDKSPPLPNLLNRQFNPAAPNRVWCGDISFIRLQDKWCYLALVVDLYSRRIIGSALSLTADAELVCRALRNALETRPRDG
RLLFHSDQGVQYKSNKYRKLLWRYGVMQSMSRRGNCLDNSPMERVFRSLKSEWLPKGGYGDFSHSVRDINQWINGYYNVYRPHTNNDGLPPCLHEEKWKQ
IIPVS

Blast result :

ORF 3

Length		Begin	End	Strand	Fusion ORF
1166 bp	388 aa	71	1236	+	Yes

ORF function : Transposase
Chemistry : DDE

ORF sequence :

MIKTRRTKRTFSPEFKLEAIEQVVKYQRDVREVALALELNPDHLRKWIRLYKQELQGIEPAGNAITPEQREIQQLKARIKRLEMEKEILKAGCRADERNP
REAVALITRLKAKWPVRVLCHLFGIHRSVYYAQVKRPVNVQRIELRSRVRAFHALSRGAAGSRAISQMLRQSGVDAGRWLARRLMRECGLTSRQPVKHHY
RVNEDKSPPLPNLLNRQFNPAAPNRVWCGDISFIRLQDKWCYLALVVDLYSRRIIGSALSLTADAELVCRALRNALETRPRDGRLLFHSDQGVQYKSNKY
RKLLWRYGVMQSMSRRGNCLDNSPMERVFRSLKSEWLPKGGYGDFSHSVRDINQWINGYYNVYRPHTNNDGLPPCLHEEKWKQIIPVS

Blast result :

Comments

ISKpn37 is 64% aa similar to ISBrsa1.

References

1] Zhou D. (2016) Direct submission to ISfinder
2] Li R., Lin D. and Chen C. (2015) Direct submission to GenBank