ISKpn38
- Family IS3
- Group IS150
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| LFAO01000049 | ND | Klebsiella pneumoniae | Klebsiella pneumoniae MGH113 Klebsiella pneumoniae plasmid pKPN_CZ |
DNA section
IS Length : 1598 bp
Ends
IR Length : 17/24
IRL : GAAGTGCGGACAAATTCCTGGACCTTCAGGAGTTAATTTATGCATTCATA
IRR : GGGGCTGCGGATGTTTTCCTGGACTGTTATGTTGTGATCCCAAGATGCTG
Comments : Non canonical ends for an IS3 family member.
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| AATTGAGCCA | CAGG | CGGACATTAC | 4 |
DNA sequence
GAAGTGCGGACAAATTCCTGGACCTTCAGGAGTTAATTTATGCATTCATACGAGGACCGGATTCGGGCCGTCGAACTCTATTACCGGTACGGAAAGAAAG
CCTCCGTTGTCGTCATGGAGCTGGGATATCCTTCCACAAAACAGCTGGGCCGTTGGGTTCGGATTTATGAAGAGAAGGGCGATTTACCCAGGGAATTAAA
ACCAAGAGAACGCTATTCACGTACACAGAAAATTGCTGCTGTTGAGCATTATCTTACTCACGGTGGTTGCCTGTCGTACACTCGCCGAGCCATCGGCTAC
CCCAGTAACGAAATTCTAAAACGCTGGATTGAAGAGTTTTACCCAAATGCGCGTCCCCTGGTCATTCGCTCAGGCACAAGCAAATGCTTCAGCCCGGAAG
AGAGGTCTCAGGCCGTCCGGGAGCTCTGTAACCGACGTGGAACCGCGCGTAAAGTTGCACAAAGCATAGGCGTCAGCGTCCCGGTCCTGTACAAATGGAA
GAAAGACCTTATCAGTGACGAGGCTTATCAATCCATGCGCAAACGAAAGGCAGCCCCTCAGGATAAAAATCAGGATGCTTTACTCGGTGAAATCCAGCGT
CTCAGGAAGCAGGTTCATCAGCTGCAGCTCGAACGTGACATACTGACAAAGGCGAATGAACTGATAAAAAAAGATCTGGGCGTCAGCTTTCTGAAACTGA
AAAACAGGGAGAAAACCCTGATAGTTGATGCCCTTAAGAAAAAGTACCCCGTTGCTGAGTTGCTAAGCGTTCTGCAACTTGCCCGCAGCTGTTATTTTTA
CCACAAAGCCAGCAAACGTCTGTACGATAAGTATGCGGAAATACGCGTGATCATGGCCGATATCTTTGAGGAGAATTACCGCTGTTACGGCTACCGGCGC
CTTCACGCGATGCTTCGCAGTAACAACAGAGTTATTTCTGAAAAGGTTGTCCGCAGACTGATGGCAGAAGAGCAGCTCGTTGTTAAGCGCACCAGGCGAC
GACGATATAACTCTTACTGTGGCGAAATCGGTCCGGCACCGGAAAATTTACTCGCCCGGGATTTTAGTTCCTGCAGGCCAAATGAGAAGTGGCTGACCGA
TATTACGGAGTTCCAGCTTCCGGCTGGAAAAGTCTATCTGTCGCCGGTTATCGACTGCTTTGATGGCCAGGTTGTAAGCTGGTCGATAGGAACACGCCCG
GACGCGACGCTGGTGAATACGATGCTCGATGACGCACTCGATACGCTCAGCGAACATGATAAACCGGTGATACACAGCGATCGTGGTGGCCATTACCGGT
GGCCGGGCTGGCTTGAGCGTATCAACACCTCCGGACTTATAAGGTCCATGTCGCGCAAAGGATGCTCGTCGGATAATGCTGCGTGTGAAGGCTTCTTCGG
GCGTATCAAAAACGAAATGTTCTATGGCAGAGACTGGGCGGGTATCACGCTGGAAAAATTTATCTGCTTCCTGGACAGGTACATACGCTGGTATAATGAG
AAACGTATCAAGCTATCATTAGGTGCAATGAGTCCTGTGAAGTACCGGCAGCATCTTGGGATCACAACATAACAGTCCAGGAAAACATCCGCAGCCCC
CCTCCGTTGTCGTCATGGAGCTGGGATATCCTTCCACAAAACAGCTGGGCCGTTGGGTTCGGATTTATGAAGAGAAGGGCGATTTACCCAGGGAATTAAA
ACCAAGAGAACGCTATTCACGTACACAGAAAATTGCTGCTGTTGAGCATTATCTTACTCACGGTGGTTGCCTGTCGTACACTCGCCGAGCCATCGGCTAC
CCCAGTAACGAAATTCTAAAACGCTGGATTGAAGAGTTTTACCCAAATGCGCGTCCCCTGGTCATTCGCTCAGGCACAAGCAAATGCTTCAGCCCGGAAG
AGAGGTCTCAGGCCGTCCGGGAGCTCTGTAACCGACGTGGAACCGCGCGTAAAGTTGCACAAAGCATAGGCGTCAGCGTCCCGGTCCTGTACAAATGGAA
GAAAGACCTTATCAGTGACGAGGCTTATCAATCCATGCGCAAACGAAAGGCAGCCCCTCAGGATAAAAATCAGGATGCTTTACTCGGTGAAATCCAGCGT
CTCAGGAAGCAGGTTCATCAGCTGCAGCTCGAACGTGACATACTGACAAAGGCGAATGAACTGATAAAAAAAGATCTGGGCGTCAGCTTTCTGAAACTGA
AAAACAGGGAGAAAACCCTGATAGTTGATGCCCTTAAGAAAAAGTACCCCGTTGCTGAGTTGCTAAGCGTTCTGCAACTTGCCCGCAGCTGTTATTTTTA
CCACAAAGCCAGCAAACGTCTGTACGATAAGTATGCGGAAATACGCGTGATCATGGCCGATATCTTTGAGGAGAATTACCGCTGTTACGGCTACCGGCGC
CTTCACGCGATGCTTCGCAGTAACAACAGAGTTATTTCTGAAAAGGTTGTCCGCAGACTGATGGCAGAAGAGCAGCTCGTTGTTAAGCGCACCAGGCGAC
GACGATATAACTCTTACTGTGGCGAAATCGGTCCGGCACCGGAAAATTTACTCGCCCGGGATTTTAGTTCCTGCAGGCCAAATGAGAAGTGGCTGACCGA
TATTACGGAGTTCCAGCTTCCGGCTGGAAAAGTCTATCTGTCGCCGGTTATCGACTGCTTTGATGGCCAGGTTGTAAGCTGGTCGATAGGAACACGCCCG
GACGCGACGCTGGTGAATACGATGCTCGATGACGCACTCGATACGCTCAGCGAACATGATAAACCGGTGATACACAGCGATCGTGGTGGCCATTACCGGT
GGCCGGGCTGGCTTGAGCGTATCAACACCTCCGGACTTATAAGGTCCATGTCGCGCAAAGGATGCTCGTCGGATAATGCTGCGTGTGAAGGCTTCTTCGG
GCGTATCAAAAACGAAATGTTCTATGGCAGAGACTGGGCGGGTATCACGCTGGAAAAATTTATCTGCTTCCTGGACAGGTACATACGCTGGTATAATGAG
AAACGTATCAAGCTATCATTAGGTGCAATGAGTCCTGTGAAGTACCGGCAGCATCTTGGGATCACAACATAACAGTCCAGGAAAACATCCGCAGCCCC
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1533 bp | 510 aa | 40 | 1572 | + | No |
Chemistry : DDE
ORF sequence :
MHSYEDRIRAVELYYRYGKKASVVVMELGYPSTKQLGRWVRIYEEKGDLPRELKPRERYSRTQKIAAVEHYLTHGGCLSYTRRAIGYPSNEILKRWIEEF
YPNARPLVIRSGTSKCFSPEERSQAVRELCNRRGTARKVAQSIGVSVPVLYKWKKDLISDEAYQSMRKRKAAPQDKNQDALLGEIQRLRKQVHQLQLERD
ILTKANELIKKDLGVSFLKLKNREKTLIVDALKKKYPVAELLSVLQLARSCYFYHKASKRLYDKYAEIRVIMADIFEENYRCYGYRRLHAMLRSNNRVIS
EKVVRRLMAEEQLVVKRTRRRRYNSYCGEIGPAPENLLARDFSSCRPNEKWLTDITEFQLPAGKVYLSPVIDCFDGQVVSWSIGTRPDATLVNTMLDDAL
DTLSEHDKPVIHSDRGGHYRWPGWLERINTSGLIRSMSRKGCSSDNAACEGFFGRIKNEMFYGRDWAGITLEKFICFLDRYIRWYNEKRIKLSLGAMSPV
KYRQHLGITT
YPNARPLVIRSGTSKCFSPEERSQAVRELCNRRGTARKVAQSIGVSVPVLYKWKKDLISDEAYQSMRKRKAAPQDKNQDALLGEIQRLRKQVHQLQLERD
ILTKANELIKKDLGVSFLKLKNREKTLIVDALKKKYPVAELLSVLQLARSCYFYHKASKRLYDKYAEIRVIMADIFEENYRCYGYRRLHAMLRSNNRVIS
EKVVRRLMAEEQLVVKRTRRRRYNSYCGEIGPAPENLLARDFSSCRPNEKWLTDITEFQLPAGKVYLSPVIDCFDGQVVSWSIGTRPDATLVNTMLDDAL
DTLSEHDKPVIHSDRGGHYRWPGWLERINTSGLIRSMSRKGCSSDNAACEGFFGRIKNEMFYGRDWAGITLEKFICFLDRYIRWYNEKRIKLSLGAMSPV
KYRQHLGITT
Blast result :
Comments
ISKpn38 is 72% aa similar to ISAtu3.
There is no translational frameshift.
This IS disrupt the ISKpn21 sequence.
There is no translational frameshift.
This IS disrupt the ISKpn21 sequence.
References
1] Dongsheng Zhou (2016) Direct submission.
2] Earl,A.M., Onderdonk,A.B., Kirby,J., Ferraro,M.J., Huang,S., Spencer,M., Fodor,A., Hooper,D., Dekker,J., O'Brien,T., Quan,V., Gombosev,A., Delaney,M., DuBois,A., Ernst,C., Kim,D.S., Rossman,W., Gohs,F., Petruso,H., Nozar,T., Mougeot,F., Manson-McGuire,A., Young,S., Abouelleil,A., Cao,P., Chapman,S.B., Griggs,A., Priest,M., Shea,T., Wortman,I., Wortman,J.R., Nusbaum,C. and Birren,B. (2015) Direct GenBank submission.
2] Earl,A.M., Onderdonk,A.B., Kirby,J., Ferraro,M.J., Huang,S., Spencer,M., Fodor,A., Hooper,D., Dekker,J., O'Brien,T., Quan,V., Gombosev,A., Delaney,M., DuBois,A., Ernst,C., Kim,D.S., Rossman,W., Gohs,F., Petruso,H., Nozar,T., Mougeot,F., Manson-McGuire,A., Young,S., Abouelleil,A., Cao,P., Chapman,S.B., Griggs,A., Priest,M., Shea,T., Wortman,I., Wortman,J.R., Nusbaum,C. and Birren,B. (2015) Direct GenBank submission.