ISAr1

  • Family IS630
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s) Riat1
Accession numberTranspositionOriginHost
K03313 ND Agrobacterium rhizogenes
Agrobacterium rhizogenes HR1
DNA section
IS Length : 1146 bp

Ends


IR Length : 11

IRL : GTGGATTGATTGAAACAAAGGAGTCCGAGTTGGGATTCCCTTTCGGTCTT
IRR : GTGGATTGATTCCAACGCTTGGTGCCCACGTTTGACGGCTGCAATGATCT

Insertion site


Left flankDirect repeatRight flankDR Length
TTGTCTTAACTAGTCCCAATTT2

DNA sequence

GTGGATTGATTGAAACAAAGGAGTCCGAGTTGGGATTCCCTTTCGGTCTTCGTCGTGCAACGATATCGTATGCGTACAGGTATCACATTTAACGTTGCTG
CGGCGGACCGAGCCCGCTTGGAAGCGATTGTTGCAGCTCCAACTTCTGCTCAGAAGCACGTGTGGCGAGCGAAGATCATCTTGATGAGCAGTGATGGCTC
GGGAACGGTCGCGATCATGGAGGCAACCGGTAAATCCAAAACCTGTGTCTGGCGCTGGCAGGAGCGCTTCATGACTGAGGGCGTCGATGGCCTTTTGCAC
GACAAGAGCAGACCGCCCGGCATTGCGCCGCTTGATGGCGAACTCGTTGAGCGTGTCGTCGCACTGACGCTTGAGACGCCTCAACAGGAAGCAACGCACT
GGACTGTTCGTGCGATGGCCAAGGCCGTTGGGATTGCAGCCTCTTCGGTTGTGAAGATCTGGCACGAGCATGGTCTTGCGCCGCATCGCTGGCGCTCTTT
CAAACTGTCGAACGACAAGGCCTTTGCCGAGAAGCTTCACGACGTCGTTGGCCTCTACGTCTCGCCACCGGCCCATGCCATTGTCCTGTCCGTCGATGAG
AAGAGCCAGATCCAGGCACTCGATCGGACGCAACCGGGACTCCCCTTGAAGAAAGGGCGCGCCGGCACAATGACCCACGATTACAAGCGCCACGGCACCA
CCACCCTATTTGCCGCCCTCAACATCCTCGACGGCTCGGTGATCGGCCGAAACATGCAGCGTCACCGGCATCAGGAGTTCATCCGTTTTCTCAACGCCAT
CGAGGCGGAACTGCCAAAGGACAAGGCCGTCCACGTCATTCTCGACAATTACGCGACCCATAAGCAGCCGAAGGTCCGCGCCTGGCTGGCAAGGCATCCG
CGCTGGACCTTCCACTTCGTCCCAACATCATGTTCATGGCTGAACGCCGTCGAGGGATTCTTCGCTAAATTGACACGTCGACGTCTGAAGCACGGTGTCT
TTCATTCCGTCGTTGACCTCCAGGCCACCATCAACCGCTTCGTCAGAGAGCATAATCAGGAACCAAAGCCGTTCATCTGGAGAGCAGATCCAGACGAGAT
CATTGCAGCCGTCAAACGTGGGCACCAAGCGTTGGAATCAATCCAC
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
1080 bp359 aa691148+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MRTGITFNVAAADRARLEAIVAAPTSAQKHVWRAKIILMSSDGSGTVAIMEATGKSKTCVWRWQERFMTEGVDGLLHDKSRPPGIAPLDGELVERVVALT
LETPQQEATHWTVRAMAKAVGIAASSVVKIWHEHGLAPHRWRSFKLSNDKAFAEKLHDVVGLYVSPPAHAIVLSVDEKSQIQALDRTQPGLPLKKGRAGT
MTHDYKRHGTTTLFAALNILDGSVIGRNMQRHRHQEFIRFLNAIEAELPKDKAVHVILDNYATHKQPKVRAWLARHPRWTFHFVPTSCSWLNAVEGFFAK
LTRRRLKHGVFHSVVDLQATINRFVREHNQEPKPFIWRADPDEIIAAVKRGHQALESI

 

Blast result :
Comments
The ORF stop codon is the TAG of the CTAG target site. ISAr1 transposase is 89 % identical to that of IS870. A duplication of two bp have been considered here, although this statement needs confirmation. Coordinates on the plasmid Ri : 5071-6217.
References
1] Slightom, J.L., Durand-Tardif, M., Jouanin, L., and Tepfer, D. (1986) . Nucleotide sequence analysis of TL-DNA of Agrobacterium rhizogenes agropine type plasmid. Identification of open reading frames. J. Biol. Chem. 261(1), 108-121.
2] Ramseier, T.M., and Gättfert, M. (1991) Arch. Microbiol. 156, 270-276.
3] van der Meer, J.R., Zehnder, A.J.B., and de Vos, W.M. (1991) J. Bacteriol. 173, 7077-7083.
4] Fournier, P., Paulus, F., and Otten, L. (1993) J. Bacteriol. 175, 3151-3160.