ISAs1

  • Family ISAs1
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s) ISAS1
Accession numberTranspositionOriginHost
L27156 Y Aeromonas salmonicida
Aeromonas salmonicida A450-ceg6
DNA section
IS Length : 1212 bp

Ends


IR Length : 22/25

IRL : CAGGGCGGGATCGCACTTTGTTAATGAAATTTGAACAGGCAGATCGTCTG
IRR : CAGGGCGGGAGCACACTTTGTTGATATTTTAACCAAAGAATAACTGAGCC

Insertion site


Left flankDirect repeatRight flankDR Length
NNNNNNNNNNGCCTACTGNNNNNNNNNN8
TCAATCCATCCAATGAAGCACGGATGAGGGGCTGGCCGAG GGGGCTGGATGTGGTTGACC0
GATGCTATACAAACAATACT GCTGGCCGAGGGTCATCACCGGAGCTGTACGATCCGACGT0
GTTGGCAGCACCCGGACTACGGCCTCATCT10
TCAGCAACCCCTTGTATCTGGCTTTCACGA10
ACGTTATCCGGGTTGCTGGCCGCTCTTGGC10
TCAGGTCTCTGCTATTCCGGTGCGGCCGGG10
ATGCTGATTAACCACACTATCAATCCGATC10
GTACAGGTTCGTCCCATCCGAGTGCGCCAG10
GTGGTCGAGGGCTTGTTTGT0

DNA sequence

CAGGGCGGGATCGCACTTTGTTAATGAAATTTGAACAGGCAGATCGTCTGTGATCTAATCGCGTCTCCATGCAGGAGGGACGCGCTATGACGTTGATTGT
ACAACTTACCCTTGTCGAAGAAACTCGCTCTGAGATCAATCGTAAACATAACCTGGTTGATGTCATGTTCCTTGTCATCAGTGCCATCATGAGTGGTGCC
GAGGGCTGGAATGACATCGAAACCTATGGCGATTCAAAGATCGATTGGCTCCGTCAGCATCGCCCCTTTGCCAACGGCATCCCGCGGCGTCACACGGTTG
CCCGCATTCTGCGCTGCATTGTCATCGATACCTTGCTTGAAGCGTTGCTGTGCTGGGTCAATGAACAGCGCACTCATCAGGGCAAACCCATCATTGCCTT
TGATGGCAAGGTGTTACGCGGCTCCTTTCGGGGAAATGCCAAGGATGCGCTTCAGTTGGTCACGGCTTACGATACCGAAAATGGGCTGGTGTTAAGCCAG
AAAGCAACCCCTAACAAGAAGGGGGAAATCGAAACCGTCAAAGATATGCTGGACATTCTTGAGCTAAAAGGAGCCGTTGTCACACTCGATGCGTTGCATT
GTCAGCGTGAGACACTGGAGAAAATCAGCGAGAAGAAGGCTCATGTAGTGGTACAGGTCAAGAATAATCAACCGAAGCTGTATCGAGCGGTTCAATCGCA
ATTCCAGTCACTGTTTGACGCCCAGAAAGAGAAGATTGTCGTCGAGCACAAAGAGAGTGGACATGGCCGCCAGGAAGAACGTTATGTATTTCAATTAAAA
GCCAAATTACCGCCAGAGTCAACGGAGAAATGGCCGACAATCCGCAGCATCATTGCCGTTGAGCGTCACCGCAGCGCCAACGGAAAAGGAACGGTAGATA
CCTCTTACTATGTCAGCTCGTTATCCCCCAAGCACAAGCTGCTAGGGCACTATATACGGCAGCACTGGCGGATAGAGAACAGCCAGCATTACATTCTTGA
TGTGGTGTTCAACGAGGATGCTTCCAGGATAGCGATGGAGGATGCGGTCGAGAATATGGCGCTATTCCGGCGGTTCGTATTGAATATCGTAAAACAGAGC
AATTGTGGTGCTCGAAGTCAGAGAAACAAGCTAAAAAGAGCCGGCTGGAATGACGACTATCGGGCTCAGTTATTCTTTGGTTAAAATATCAACAAAGTGT
GCTCCCGCCCTG
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
1098 bp365 aa871184+No
ORF function : Transposase
Chemistry : Unknow

ORF sequence :

MTLIVQLTLVEETRSEINRKHNLVDVMFLVISAIMSGAEGWNDIETYGDSKIDWLRQHRPFANGIPRRHTVARILRCIVIDTLLEALLCWVNEQRTHQGK
PIIAFDGKVLRGSFRGNAKDALQLVTAYDTENGLVLSQKATPNKKGEIETVKDMLDILELKGAVVTLDALHCQRETLEKISEKKAHVVVQVKNNQPKLYR
AVQSQFQSLFDAQKEKIVVEHKESGHGRQEERYVFQLKAKLPPESTEKWPTIRSIIAVERHRSANGKGTVDTSYYVSSLSPKHKLLGHYIRQHWRIENSQ
HYILDVVFNEDASRIAMEDAVENMALFRRFVLNIVKQSNCGARSQRNKLKRAGWNDDYRAQLFFG

 

Blast result :
Comments
Aeromonas salmonicida is a fish pathogen whose major virulence factor is a tetragonal paracrystalline surface protein array (A-layer), encoded by the vapA gene. ISAs1 has been found as an insertion in the vapA gene after growing the strain at 30¡ C (Is transposition triggered by high temperature ?). This is the first example of IS-dependent mutagenesis of an S-layer. A 42 kDa protein corresponding to the putative ISAs1 transposase has been observed in Escherichia coli minicells.
This file was updated with the complete genome of Aeromonas salmonicida subsp. salmonicida A449 plasmid 4 (Accession number : CP000645).

File updated the March 03 2015 with the information provided by F. Pfeiffer.
References
1] Gustafson, C.E., Chu, S. and Trust, T.J. (1994) J. Mol. Biol. 237, 452-463.